[Objective] The aim was to study the expression of cold resistant gene CAS19 of Gongnong No.2 Medicago sativa L. in tobacco. [Method] A pair of primers was designed according to nucleotide sequences of cold resistant ...[Objective] The aim was to study the expression of cold resistant gene CAS19 of Gongnong No.2 Medicago sativa L. in tobacco. [Method] A pair of primers was designed according to nucleotide sequences of cold resistant gene CAS19 of M. sativa,and then RT-PCR was used to amplify the protein gene of CAS19,which was then cloned into pMD18-T vector and subcloned into expression vector PBI121. The recombination expression plasmid PBCAS was constructed. And then it was transferred into tobacco genome via Agrobacterium,and Southern-blotting analysis was used for detecting transgenic plants. [Result] CAS19 gene was integrated into the tobacco genome and highly expressed. [Conclusion] This study had provided theoretical basis for further exploring the expression mechanism of cold resistant gene CAS19 in tobacco.展开更多
目的:探索术前血清CA19-9与腹膜假黏液瘤(pseudomyxoma peritonei,PMP)病理级别的关系,分析CA19-9对预后的影响。方法:回顾性分析2004年10月至2024年10月于本团队初次行肿瘤细胞减灭术联合腹腔热灌注化疗(cytoreductive surgery plus hy...目的:探索术前血清CA19-9与腹膜假黏液瘤(pseudomyxoma peritonei,PMP)病理级别的关系,分析CA19-9对预后的影响。方法:回顾性分析2004年10月至2024年10月于本团队初次行肿瘤细胞减灭术联合腹腔热灌注化疗(cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy,CRS+HIPEC)的321例PMP患者的临床资料,受试者工作曲线用于分析CA19-9对病理级别的鉴别价值,K-M法评估不同水平CA19-9对总体生存期的影响。结果:46.1%(148/321)的患者术前CA19-9水平升高,且与病理级别相关;术前CA19-9>693.3 U/mL时,病理级别更倾向于腹膜高级别黏液癌伴印戒细胞(曲线下面积:0.67,95%CI:0.54~0.80,P=0.021);术前CA19-9是影响PMP患者总生存期的独立危险因素(升高组vs.正常组,中位生存期53.62个月vs.未达到,P<0.001)。结论:初次行CRS+HIPEC的PMP患者,其术前血清CA19-9水平与病理级别相关,是影响总生存期的不良因素。展开更多
基金Supported by National High Technology Research and Development Program of China(2008AA10Z224)National Natural Science Foundation of China (30471229)~~
文摘[Objective] The aim was to study the expression of cold resistant gene CAS19 of Gongnong No.2 Medicago sativa L. in tobacco. [Method] A pair of primers was designed according to nucleotide sequences of cold resistant gene CAS19 of M. sativa,and then RT-PCR was used to amplify the protein gene of CAS19,which was then cloned into pMD18-T vector and subcloned into expression vector PBI121. The recombination expression plasmid PBCAS was constructed. And then it was transferred into tobacco genome via Agrobacterium,and Southern-blotting analysis was used for detecting transgenic plants. [Result] CAS19 gene was integrated into the tobacco genome and highly expressed. [Conclusion] This study had provided theoretical basis for further exploring the expression mechanism of cold resistant gene CAS19 in tobacco.
文摘目的 探讨微卫星不稳定性(microsatellite instability,MSI)、术前D-二聚体(D-dimer,DD)和CA19-9水平对结直肠癌(colorectal cancer,CRC)根治术患者的预测价值。方法 回顾性分析2020年1月至2021年12月于广西医科大学第一附属医院及桂林市人民医院行根治性手术的81例CRC患者的一般临床资料、肿瘤组织有无MSI、术前DD和CA19-9水平,筛选影响预后的因素并进行评价。结果 复发组与无复发组患者的MSI发生率、术前DD和CA19-9水平差异有统计学意义(P<0.05);死亡组与生存组患者的MSI发生率、术前DD和CA19-9水平差异有统计学意义(P<0.05)。单因素及多因素分析结果显示MSI是CRC术后复发的保护因素(P=0.018);术前DD和CA19-9高表达是CRC术后复发的独立危险因素(P=0.020,P=0.042);MSI是CRC术后死亡的保护因素(P=0.036);术前DD和CA19-9高表达是CRC术后死亡的独立危险因素(P=0.010,P=0.017)。受试者操作特征曲线显示MSI、DD、CA19-9预测复发的曲线下面积(area under the curve,AUC)分别为0.643、0.599、0.644,MSI、DD、CA19-9单独用于预测死亡的AUC分别为0.646、0.642、0.693;三者联合预测复发及死亡的AUC分别为0.776、0.825。结论 CRC根治术患者的MSI是预后的保护因素、术前DD和CA19-9高表达是预后的危险因素,三者联合检测可优化CRC根治术预后评估的准确性。
文摘目的:探索术前血清CA19-9与腹膜假黏液瘤(pseudomyxoma peritonei,PMP)病理级别的关系,分析CA19-9对预后的影响。方法:回顾性分析2004年10月至2024年10月于本团队初次行肿瘤细胞减灭术联合腹腔热灌注化疗(cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy,CRS+HIPEC)的321例PMP患者的临床资料,受试者工作曲线用于分析CA19-9对病理级别的鉴别价值,K-M法评估不同水平CA19-9对总体生存期的影响。结果:46.1%(148/321)的患者术前CA19-9水平升高,且与病理级别相关;术前CA19-9>693.3 U/mL时,病理级别更倾向于腹膜高级别黏液癌伴印戒细胞(曲线下面积:0.67,95%CI:0.54~0.80,P=0.021);术前CA19-9是影响PMP患者总生存期的独立危险因素(升高组vs.正常组,中位生存期53.62个月vs.未达到,P<0.001)。结论:初次行CRS+HIPEC的PMP患者,其术前血清CA19-9水平与病理级别相关,是影响总生存期的不良因素。
