The current investigation focuses on intertwined relationships of ecology and aquaculture for the benefit of farmers involved in fish farming practices.The study evaluated glucosinolate reduction in black,brown,and wh...The current investigation focuses on intertwined relationships of ecology and aquaculture for the benefit of farmers involved in fish farming practices.The study evaluated glucosinolate reduction in black,brown,and white mustard meals as fish feed ingredients for Indian Major Carps.Fish were fed with 10%mustard meal-supplemented diets in three forms:Raw(R),Anti-nutritional Rich(AR),and Anti-nutritional Lowered(AL),alongside a control group using floating feed.The three-month indoor experiment(September-November 2023)was conducted in FRP tanks with triplicate treatments.Blood analysis revealed compromised health in AR-fed carps,with reduced hemoglobin levels in rohu,catla and mrigal and elevated total leukocyte counts indicating inflammation in all the three carps studied here.Liver function was impaired in AR-fed fish,shown by increased alanine transaminase levels,highest in rohu followed by mrigal and catla.Histopathological examination of AR-fed carps liver tissue revealed necrotic spots,deformed hepatocytes,and significant vacuolation.In contrast,AL-fed fish demonstrated improved health parameters through Complete Blood Count analysis,liver function tests,and histo-pathological observations,suggesting successful reduction of anti-nutritional factors in the processed mustard meals.In near future,replacement of unprocessed seed meal with processed seed meal will lead to economic gains in fish farming.展开更多
Silver carp mediated biological control techniques are often advocated for controlling cyanobacteria blooms in eutrophic water,which are often enriched with arsenic(As).How-ever,the transfer and fate of As during the ...Silver carp mediated biological control techniques are often advocated for controlling cyanobacteria blooms in eutrophic water,which are often enriched with arsenic(As).How-ever,the transfer and fate of As during the biological control of cyanobacteria blooms by silver carp in As-rich eutrophic water remain unclear.Based on the simulated ecosystem experiment,the accumulation of As in silver carp and the transfer and fate of As in the water-algae-silver carp system during Microcystis aeruginosa blooms controlled by silver carp were investigated.Microcystis aeruginosa showed high tolerance to As(V).The accumulation of As in different tissues of silver carp was different,as follows:intestine>liver>gill>skin>muscle.After silver carp ingested As-rich Microcystis aeruginosa,As accumulation in the intestine,liver,gill,and skin of silver carp was enhanced under the action of digestion and skin contact.Compared with the system without algal,As accumulation in the intestine,liver,gill,and skin of silver carp increased by 1.1,3.3,3.3,and 9.6 times,respectively,after incubation for 30 days in the system with Microcystis aeruginosa,while the accumulation of As in the muscle was only slightly increased by 0.56 mg/kg.This work revealed the transfer and fate of As during algal control by silver carp,elucidated the accumulation mechanism of As in water-algae-silver carp system,enriched our understanding of As bioaccumulation and transformation in As-rich eutrophication water,and provided a scientific basis for as-sessing and predicting As migration and enrichment in water-algae-silver carp system.展开更多
Brain-controlled technology is the key technology in biological control,and the corresponding relationship between animal brain nuclei and motor behavior is the core.The purpose of this study was to explore the motor ...Brain-controlled technology is the key technology in biological control,and the corresponding relationship between animal brain nuclei and motor behavior is the core.The purpose of this study was to explore the motor nuclei of the medulla oblongata in carp.The carps were subjected to electrical stimulation and chemical stimulation experiments,respectively,in the water-free state,and the effective chemical stimulation sites were injected with the pontamine sky blue solution.The brain tissue sections were obtained by paraffin tissue section technology and the neutral red staining method.By comparing the positions of the brain nuclei shown in earlier studies,the motor nerve nuclei in the medulla oblongata were identified.The brain electrode was implanted into the motor nucleus of the medulla oblongata,and the underwater control experiment and behavioral tests were carried out with different electrical stimulation parameters.The results showed that the abducens nucleus(NVI)was the motor nucleus that controls the ipsilateral steering,and the facial nucleus(NVII)was the motor nucleus that controls the forward movement.By adjusting the stimulation voltage and the stimulation pulse number,the carp can be stably controlled to achieve a left–right steering motion of 30°–180°and a forward motion of more than 80 cm/s.This study indicated that the quantitative control of the steering and forward behavior of the carp can be achieved by electrical stimulation of the NVI and NVII,which provided a certain experimental basis for the accurate control of the carp robot.展开更多
Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated ...Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella)IFN-υ(CiIFN-υ)both in vivo and in vitro.The CiIFN-υgene was first identified and characterized in grass carp.Subsequently,the immune expression of CiIFN-υsignificantly increased following bacterial challenge,indicating its response to bacterial infections.The eukaryotic recombinant expression plasmid of CiIFN-υwas then constructed and transfected into fathead minnow(FHM)cells.Supernatants were collected and incubated with four bacterial strains,followed by plate spreading and colony counting.Results indicated that CiIFN-υexhibited more potent antibacterial activity against gram-negative bacteria compared to gram-positive bacteria and aggregated gram-negative bacteria but not gram-positive bacteria.In vivo experiments further confirmed the antibacterial function,showing high survival rates,low tissue edema and damage,reduced tissue bacterial load,and elevated proinflammatory response at the early stages of bacterial infection.In addition,the antiviral function of CiIFN-υwas confirmed through in vitro and in vivo experiments,including crystal violet staining,survival rates,tissue viral burden,and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR).This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υpossesses dual functions against bacterial and viral infections.展开更多
Carp is a temperate freshwater fish native to Asia,distributed in all regions of the world except Australia and South America.With the improvement of comprehensive and healthy breeding technology of carp,the unit yiel...Carp is a temperate freshwater fish native to Asia,distributed in all regions of the world except Australia and South America.With the improvement of comprehensive and healthy breeding technology of carp,the unit yield has been greatly increased mainly due to the exten-sive use of compound feed.In this study,the nutritional requirements of carp were summarized from the aspects of protein,amino acids,fat,carbohydrate,calcium and phosphorus,vitamins and taurine.This study provides a certain theoretical reference for scientific formula of carp feed.展开更多
Multivariate analysis was adopted to analyze 30 morphometrical characteristics of 121 one-year-old juvenile silver carp （Hypophthalmichthys molitrix） and bighead carp （Aristichthys nobilis） bred during the 1950s ...Multivariate analysis was adopted to analyze 30 morphometrical characteristics of 121 one-year-old juvenile silver carp （Hypophthalmichthys molitrix） and bighead carp （Aristichthys nobilis） bred during the 1950s （＂the former population＂） and 2008 （＂the current population＂） and collected from the middle reach of the Yangtze River. The average discriminant accuracies of the former and current silver and bighead carp population were 94.2% and 98.0%, respectively. Discriminant analysis also revealed that significant differences in morphology occurred between the former and current populations of both carp in overall characteristics. One-way analysis of variance indicated that between former and current populations, silver carp showed highly significant differences （P0.01） in twelve of their characteristics and significant differences （P0.05） in eight of their characteristics, while bighead carp showed highly significant differences （P0.01） in eight of their characteristics and significant differences （P0.05） in eight of their characteristics. Six head morphology variables of the current silver and bighead carp were significantly or highly significantly larger than the former populations; fourteen characteristics of silver carp and ten characteristics of bighead carp of the current populations, mainly reflecting truck and tail morphology, were significantly or very significantly smaller than the former populations. Our results indicate that silver and bighead carp have developed a larger head and smaller truck and tail during the last 50 years. Due to such morphological changes, it seems apparent that the heads of these fish species need to be considered in regards to human diets, particularly in relation to economic and nutritious value.展开更多
The genomes of three groups of grass carp, namely the Xiangjiang River grass carp group （Xiangjiang group）, a one-generation artificially induced meio-gynogenetic grass carp group （meio-gynogenetic-1 group）, and a...The genomes of three groups of grass carp, namely the Xiangjiang River grass carp group （Xiangjiang group）, a one-generation artificially induced meio-gynogenetic grass carp group （meio-gynogenetic-1 group）, and a two-generation artificially induced meio-gynogenetic grass carp group （meio-gynogenetic-2 group）, were comparatively analyzed with microsatellite markers. Genetic polymorphism had been observed in the Xiangjiang group and most of the examined loci had more than two alleles. But the degree of genetic diversity was not very high. Although all the examined genetic loci in the analyzed individuals were in homozygous state, the genotypes of different individuals of the group were not identical in the meio-gynogenetic-1 group. In the meio-gynogenetic-2 group, not only the examined genetic loci of each individual were homozygous but also the genotypes of all the analyzed individuals of the group were the same. These results suggested that the examined meio-gynogenetic-2 group is a homozygous group and homozygous clone could be produced by continuous artificial induction of gynogenesis for two generations. It was found that the polymorphism existed not only at the allele level but also at the locus level; many alleles of the microsatellite loci and some of the microsatellite loci had been lost during the process of artificial gynogenesis. Therefore, both protection of the diversity of natural grass carp resource and selection of homozygous traits with desired economic genotypes are very important aspects for grass carp breeding.展开更多
Thirty microsatellite loci were used for analyzing six wild populations of common carp （Cyprinus carpio L.）. Observed （Ho） and expected （He） heterozygosity values, polymorphic information content （PIC）, and nu...Thirty microsatellite loci were used for analyzing six wild populations of common carp （Cyprinus carpio L.）. Observed （Ho） and expected （He） heterozygosity values, polymorphic information content （PIC）, and number of effective alleles （Ae） were all detected. Genetic similarity index and genetic distance were computed based on the allele frequency. The Hardy-Weinberg Equilibrium was checked according to the test of χ^2. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm. A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package （version 3.63）. There were totally 8,136 fragments ranging from 125 bp to 414 bp in length. Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations. The average number of alleles in each locus was seven. The result showed that 1） the level of genetic variability was moderate in the six populations. Polymorphic information contents of the six wild common carp populations were 0.44, 0.52, 0.53, 0.57, 0.63, and 0.64 respectively. Effective alleles were from 1.04 to 4.72, the average numbers in each population were 2.19, 2.60, 2.42, 2.43, 2.45, and 2.33. The average expected heterozygosity values were 0.50, 0.59, 0.56, 0.56, 0.57, and 0.54 respectively; 2） the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688, and it came from the populations of BR and HN. There was a correlation between the clustering result and the geographical distribution.展开更多
[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR （Yangtze River stock ♀×Yangtze River...[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR （Yangtze River stock ♀×Yangtze River stock ♂ ）, ZR （Zhujiang River stock ♀ ×Zhujiang River stock♂ ） and hybrids F1 （ Yangtze River stock ♀ × Zhujiang River stock ♂ ） were established, and their growth or morphological data were also measured. [ Result] The orders of body weight, standard length and absolute growth rate were F1 〉 ZR 〉 YR. No significant difference was found in body weight or standard length of 50 day-old generations （ P〉0. 05）, while there was an extremely significantly difference in those of 170 day-old generations （ P 〈0.01 ）. However, the absolute growth rate of F1 was 20.00% and 50.00% higher than that of ZR and YR respectively, and no significant difference was found between F1 and ZR （ P 〉 0.05）, but significant difference between F1 and YR （ P 〈 0.05）. F1 showed a significant hybrid vigor with rate of 20.09%. There was no significant difference among three inbred generations in standard length/total length （ P 〉 0.05）, while significant difference in head length/total length, body height/standard length and body width/standard length （ P 〈 0.05）, which indicated that F1 had the characteristics of shorter head, higher and wider body. [ Conclusion]F1 has advantages in growth performance and morphological characteristics.展开更多
In order to determine the applicability of microsatellite primers developed from common carp （ Cyprinuscario ） for genomic analysis in mud carp （ Cirrhina molitorella ）, 24 primer pairs from common carp were desig...In order to determine the applicability of microsatellite primers developed from common carp （ Cyprinuscario ） for genomic analysis in mud carp （ Cirrhina molitorella ）, 24 primer pairs from common carp were designed to amplify microsatellite loci in the mud carp containing CA, GA, AT and GGGA sequences. Thirteen primers （54%） successfully amplified specific products in the mud carp and 11 primers （48%） showed high polymorphism in the mud carp population. The results indicated that the average number of alleles per locus in the mud carp stocks was 5.2. Average heterozygosity （Ho）, unbiased expected heterozygosity （He） and polymorphism information content （PIC） in the wild population were 0.61 ± 0.2, 0.8 ± 0.09 and 0.72 ± 0.1 respectively. Several Hardy-Weinberg departure value were significandy departed from Hardy-Weinberg equilibrium. The study showed that microsatellite primers from a species of Cyprinidae can be used for mud carp genetic analysis without much cost or time input.展开更多
The aim was to protect the Henan Yellow River carp germplasm resources and provide a scientific basis for the meat quality improvement of Yellow River carps. With artificially farmed and wild Henan Yellow River carps ...The aim was to protect the Henan Yellow River carp germplasm resources and provide a scientific basis for the meat quality improvement of Yellow River carps. With artificially farmed and wild Henan Yellow River carps as the research objects, comparative study on muscle fiber diameter and density, routine nutritional composition, calcium and phosphorus contents and amino acids composition was conducted between the two groups of carps. The results showed that the moisture content was significantly higher （P 〈0.05）, the crude fat and crude protein contents were significantly lower （P〈0.05）, the essential amino acids and total amino acids contents were lower （P〉0.05）, the phosphorus content was higher （P 〉0.05）, and the calcium content was lower （P〉0.05） in the wild group compared with those in the farming group. The analysis of muscle fiber characteristics showed that there were significant differences in the average muscle fiber diameter （P〈0.05） and muscle fiber density （P〈0.01） between the two groups of Henan Yellow River carps.展开更多
[ Objective] The study aimed to discuss the toxicity effect of abamectin and ehlorpyrifos on grass carp （ Ctenopharyngdon idllus ） and their interrela- tions. [ Method] Taking healthy and active grass carp as the ob...[ Objective] The study aimed to discuss the toxicity effect of abamectin and ehlorpyrifos on grass carp （ Ctenopharyngdon idllus ） and their interrela- tions. [ Method] Taking healthy and active grass carp as the object, the acute toxicity test of single pesticide and the combined toxicity test of two pesticides on grass carp were carried out. [ Result] The LC50 of abamectin and chlorpyrifes and the mixture of two pesticides against grass carp at 24, 48, 72 and 96 h were as follows： abamectin： 0.54, 0.49, 0. 17 and 0.10 rag/L; chlorpyrifes ： 0.29, 0.21, 0.12 and 0.05 rag/L; the mixture of two pesticides ： 1.22, 1.08, 0.99 and 0. 86 mg/L. The safe concentration （SC） of ahamectin, chlorpyrifos and the mixture of two pesticides were 0.010, 0.005 and 0.086 rag/L, respectively. The tox- icity of the pesticides in sequence was ablorpyrifos 〉 ahamectin 〉 the mixture of two pesticides. [ Conclusion ] Different concentrations of abamectin, chlorpyrifos and the mixture of two pesticides had remarkable effect on the growth of grass carp, the higher the concentration was, the greater the toxicity effect was. Ahamectin at low toxicity intensity had certain relief function on the toxicity of ehlorpyrifos.展开更多
During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per gene...During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp.展开更多
[Objective] The curative effect of onion on bacterial rotted gill disease in grass carp was researched [Method] The combination method of taking medicine through oral and spraying was used to cure sick grass carp for ...[Objective] The curative effect of onion on bacterial rotted gill disease in grass carp was researched [Method] The combination method of taking medicine through oral and spraying was used to cure sick grass carp for 1 period of treatment in room under artificial conditions. [Result] Different concentrations of onion generated different cure rates. When the combination was adding 1.0% - 2.0% medicine into feed and spraying 2.0 g,/m3 - 5.0 g,/m3, the curative result was the best with cure rate was 70% -90% [ Conclusion] The onion was effective on curing bacterial rotted gill disease in grass carp and could be taken as curative medicine.展开更多
With fresh-cut grass carp belly as the test material, the antibacterial con- ditions of a disinfection agent sodium hypochlorite were optimized. In addition, the shelf lives of various grass carp products were compare...With fresh-cut grass carp belly as the test material, the antibacterial con- ditions of a disinfection agent sodium hypochlorite were optimized. In addition, the shelf lives of various grass carp products were compared after disinfection, so as to provide certain basis for the processing of fresh-cut grass carp products. The re- sults showed that the optimal disinfection conditions of sodium hypochlorite were as follows： concentration 300 mg/L, soak time 5 min and solid-liquid ratio 1 g ： 5 ml. Under the optimal disinfection conditions, the inhibition rates of total bacteria and Pseudomonas reached 83% and 81%, respectively. The shelf life of refrigerated fresh-cut grass carp belly disinfected by sodium hypochlorite and packed in bag filled with gases could be extended as long as 11 d, which was increased by 5 d （83%） compared with that in the control group. The disinfection by sodium hypochlorite could significantly reduce the initial bacterial colony number and improve the sensory quality of fresh-cut grass carp belly, as well as extend the shelf life of refrigerated and modified gas-packed grass carp belly.展开更多
[Objective] The aim was to discuss the morphological differences of four carp populations.[Method] Based on traditional morphological data（including countable and measurable characters） and framework data,the morpho...[Objective] The aim was to discuss the morphological differences of four carp populations.[Method] Based on traditional morphological data（including countable and measurable characters） and framework data,the morphological differences and phylogenesis of Yellow River carp population（YR）,wild four-naris carp population（WF）,farmed four-naris carp population（FF） and carp population from Suzhou section of Wusong River（WR） were studied by means of multivariate analysis.[Result] There was no significant difference（P〉0.05） in countable characters among four carp populations.Discriminant analysis showed the discriminating accuracy of YR,WF,FF and WR population were 87.5%,78.1%,73.5% and 96.8% respectively,and the synthetic discriminating accuracy of four populations was up to 83.7%.In addition,cluster analysis revealed that YR population and WF population clustered together,while FF population and WR population clustered together.Principal component analysis indicated that the morphological differences among four populations mainly came from the head,tail and vertical axis of fish.[Conclusion] The study could provide theoretical foundations for the purification and rejuvenation of rare four-naris carp.展开更多
文摘The current investigation focuses on intertwined relationships of ecology and aquaculture for the benefit of farmers involved in fish farming practices.The study evaluated glucosinolate reduction in black,brown,and white mustard meals as fish feed ingredients for Indian Major Carps.Fish were fed with 10%mustard meal-supplemented diets in three forms:Raw(R),Anti-nutritional Rich(AR),and Anti-nutritional Lowered(AL),alongside a control group using floating feed.The three-month indoor experiment(September-November 2023)was conducted in FRP tanks with triplicate treatments.Blood analysis revealed compromised health in AR-fed carps,with reduced hemoglobin levels in rohu,catla and mrigal and elevated total leukocyte counts indicating inflammation in all the three carps studied here.Liver function was impaired in AR-fed fish,shown by increased alanine transaminase levels,highest in rohu followed by mrigal and catla.Histopathological examination of AR-fed carps liver tissue revealed necrotic spots,deformed hepatocytes,and significant vacuolation.In contrast,AL-fed fish demonstrated improved health parameters through Complete Blood Count analysis,liver function tests,and histo-pathological observations,suggesting successful reduction of anti-nutritional factors in the processed mustard meals.In near future,replacement of unprocessed seed meal with processed seed meal will lead to economic gains in fish farming.
基金supported by the National Natural Science Foundation of China(Nos.41572230 and 41172219)the Grant for Innovative Research Groups of the National Natural Sci-ence Foundation of China(No.41521001)+1 种基金the Special Scien-tific Research Project of Hanzhong City-Shaanxi University of Technology Co-construction State Key Laboratory(No.SXJ-2106)the Scientific Research Foundation of State Key Laboratory of Qinba Bio-Resource and Ecological Environment(No.SXC-2105).
文摘Silver carp mediated biological control techniques are often advocated for controlling cyanobacteria blooms in eutrophic water,which are often enriched with arsenic(As).How-ever,the transfer and fate of As during the biological control of cyanobacteria blooms by silver carp in As-rich eutrophic water remain unclear.Based on the simulated ecosystem experiment,the accumulation of As in silver carp and the transfer and fate of As in the water-algae-silver carp system during Microcystis aeruginosa blooms controlled by silver carp were investigated.Microcystis aeruginosa showed high tolerance to As(V).The accumulation of As in different tissues of silver carp was different,as follows:intestine>liver>gill>skin>muscle.After silver carp ingested As-rich Microcystis aeruginosa,As accumulation in the intestine,liver,gill,and skin of silver carp was enhanced under the action of digestion and skin contact.Compared with the system without algal,As accumulation in the intestine,liver,gill,and skin of silver carp increased by 1.1,3.3,3.3,and 9.6 times,respectively,after incubation for 30 days in the system with Microcystis aeruginosa,while the accumulation of As in the muscle was only slightly increased by 0.56 mg/kg.This work revealed the transfer and fate of As during algal control by silver carp,elucidated the accumulation mechanism of As in water-algae-silver carp system,enriched our understanding of As bioaccumulation and transformation in As-rich eutrophication water,and provided a scientific basis for as-sessing and predicting As migration and enrichment in water-algae-silver carp system.
基金supported by the Project of National Natural Science Foundation of China(project number:61573305)Project of Natural Science Foundation of Hebei Province of China(project number:F2022203038,F2019203511)National High-Tech Research and Development Plan of China(863 Plan)Project(2013AA****)Fund.
文摘Brain-controlled technology is the key technology in biological control,and the corresponding relationship between animal brain nuclei and motor behavior is the core.The purpose of this study was to explore the motor nuclei of the medulla oblongata in carp.The carps were subjected to electrical stimulation and chemical stimulation experiments,respectively,in the water-free state,and the effective chemical stimulation sites were injected with the pontamine sky blue solution.The brain tissue sections were obtained by paraffin tissue section technology and the neutral red staining method.By comparing the positions of the brain nuclei shown in earlier studies,the motor nerve nuclei in the medulla oblongata were identified.The brain electrode was implanted into the motor nucleus of the medulla oblongata,and the underwater control experiment and behavioral tests were carried out with different electrical stimulation parameters.The results showed that the abducens nucleus(NVI)was the motor nucleus that controls the ipsilateral steering,and the facial nucleus(NVII)was the motor nucleus that controls the forward movement.By adjusting the stimulation voltage and the stimulation pulse number,the carp can be stably controlled to achieve a left–right steering motion of 30°–180°and a forward motion of more than 80 cm/s.This study indicated that the quantitative control of the steering and forward behavior of the carp can be achieved by electrical stimulation of the NVI and NVII,which provided a certain experimental basis for the accurate control of the carp robot.
基金supported by the Biological Breeding-Major Projects(2023ZD04065)。
文摘Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella)IFN-υ(CiIFN-υ)both in vivo and in vitro.The CiIFN-υgene was first identified and characterized in grass carp.Subsequently,the immune expression of CiIFN-υsignificantly increased following bacterial challenge,indicating its response to bacterial infections.The eukaryotic recombinant expression plasmid of CiIFN-υwas then constructed and transfected into fathead minnow(FHM)cells.Supernatants were collected and incubated with four bacterial strains,followed by plate spreading and colony counting.Results indicated that CiIFN-υexhibited more potent antibacterial activity against gram-negative bacteria compared to gram-positive bacteria and aggregated gram-negative bacteria but not gram-positive bacteria.In vivo experiments further confirmed the antibacterial function,showing high survival rates,low tissue edema and damage,reduced tissue bacterial load,and elevated proinflammatory response at the early stages of bacterial infection.In addition,the antiviral function of CiIFN-υwas confirmed through in vitro and in vivo experiments,including crystal violet staining,survival rates,tissue viral burden,and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR).This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υpossesses dual functions against bacterial and viral infections.
文摘Carp is a temperate freshwater fish native to Asia,distributed in all regions of the world except Australia and South America.With the improvement of comprehensive and healthy breeding technology of carp,the unit yield has been greatly increased mainly due to the exten-sive use of compound feed.In this study,the nutritional requirements of carp were summarized from the aspects of protein,amino acids,fat,carbohydrate,calcium and phosphorus,vitamins and taurine.This study provides a certain theoretical reference for scientific formula of carp feed.
基金National Natural Science Foundation of China (30630051)Aquaculture E-Institute of Shanghai Universities(03E009)Shanghai Leading Academic Discipline Project(S30701)
文摘Multivariate analysis was adopted to analyze 30 morphometrical characteristics of 121 one-year-old juvenile silver carp （Hypophthalmichthys molitrix） and bighead carp （Aristichthys nobilis） bred during the 1950s （＂the former population＂） and 2008 （＂the current population＂） and collected from the middle reach of the Yangtze River. The average discriminant accuracies of the former and current silver and bighead carp population were 94.2% and 98.0%, respectively. Discriminant analysis also revealed that significant differences in morphology occurred between the former and current populations of both carp in overall characteristics. One-way analysis of variance indicated that between former and current populations, silver carp showed highly significant differences （P0.01） in twelve of their characteristics and significant differences （P0.05） in eight of their characteristics, while bighead carp showed highly significant differences （P0.01） in eight of their characteristics and significant differences （P0.05） in eight of their characteristics. Six head morphology variables of the current silver and bighead carp were significantly or highly significantly larger than the former populations; fourteen characteristics of silver carp and ten characteristics of bighead carp of the current populations, mainly reflecting truck and tail morphology, were significantly or very significantly smaller than the former populations. Our results indicate that silver and bighead carp have developed a larger head and smaller truck and tail during the last 50 years. Due to such morphological changes, it seems apparent that the heads of these fish species need to be considered in regards to human diets, particularly in relation to economic and nutritious value.
基金This work was supported by National Natural Sciences Foundation of China (No. 39830300 )the Ministry of Education (No. 20065-14)
文摘The genomes of three groups of grass carp, namely the Xiangjiang River grass carp group （Xiangjiang group）, a one-generation artificially induced meio-gynogenetic grass carp group （meio-gynogenetic-1 group）, and a two-generation artificially induced meio-gynogenetic grass carp group （meio-gynogenetic-2 group）, were comparatively analyzed with microsatellite markers. Genetic polymorphism had been observed in the Xiangjiang group and most of the examined loci had more than two alleles. But the degree of genetic diversity was not very high. Although all the examined genetic loci in the analyzed individuals were in homozygous state, the genotypes of different individuals of the group were not identical in the meio-gynogenetic-1 group. In the meio-gynogenetic-2 group, not only the examined genetic loci of each individual were homozygous but also the genotypes of all the analyzed individuals of the group were the same. These results suggested that the examined meio-gynogenetic-2 group is a homozygous group and homozygous clone could be produced by continuous artificial induction of gynogenesis for two generations. It was found that the polymorphism existed not only at the allele level but also at the locus level; many alleles of the microsatellite loci and some of the microsatellite loci had been lost during the process of artificial gynogenesis. Therefore, both protection of the diversity of natural grass carp resource and selection of homozygous traits with desired economic genotypes are very important aspects for grass carp breeding.
基金This work is supported by the Key Project of Chinese National Program for Fundamental Research and Development (No. 2004CB- 117405)Chinese commonweal Program (No. 2005DIB4J024).
文摘Thirty microsatellite loci were used for analyzing six wild populations of common carp （Cyprinus carpio L.）. Observed （Ho） and expected （He） heterozygosity values, polymorphic information content （PIC）, and number of effective alleles （Ae） were all detected. Genetic similarity index and genetic distance were computed based on the allele frequency. The Hardy-Weinberg Equilibrium was checked according to the test of χ^2. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm. A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package （version 3.63）. There were totally 8,136 fragments ranging from 125 bp to 414 bp in length. Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations. The average number of alleles in each locus was seven. The result showed that 1） the level of genetic variability was moderate in the six populations. Polymorphic information contents of the six wild common carp populations were 0.44, 0.52, 0.53, 0.57, 0.63, and 0.64 respectively. Effective alleles were from 1.04 to 4.72, the average numbers in each population were 2.19, 2.60, 2.42, 2.43, 2.45, and 2.33. The average expected heterozygosity values were 0.50, 0.59, 0.56, 0.56, 0.57, and 0.54 respectively; 2） the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688, and it came from the populations of BR and HN. There was a correlation between the clustering result and the geographical distribution.
基金Supported by National Freshwater Fish Industrial Technology System ProjectKey Discipline Construction Project of Shanghai(Y1101)Key Project of Science Committee in Shanghai(06DJ14003)~~
文摘[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR （Yangtze River stock ♀×Yangtze River stock ♂ ）, ZR （Zhujiang River stock ♀ ×Zhujiang River stock♂ ） and hybrids F1 （ Yangtze River stock ♀ × Zhujiang River stock ♂ ） were established, and their growth or morphological data were also measured. [ Result] The orders of body weight, standard length and absolute growth rate were F1 〉 ZR 〉 YR. No significant difference was found in body weight or standard length of 50 day-old generations （ P〉0. 05）, while there was an extremely significantly difference in those of 170 day-old generations （ P 〈0.01 ）. However, the absolute growth rate of F1 was 20.00% and 50.00% higher than that of ZR and YR respectively, and no significant difference was found between F1 and ZR （ P 〉 0.05）, but significant difference between F1 and YR （ P 〈 0.05）. F1 showed a significant hybrid vigor with rate of 20.09%. There was no significant difference among three inbred generations in standard length/total length （ P 〉 0.05）, while significant difference in head length/total length, body height/standard length and body width/standard length （ P 〈 0.05）, which indicated that F1 had the characteristics of shorter head, higher and wider body. [ Conclusion]F1 has advantages in growth performance and morphological characteristics.
文摘In order to determine the applicability of microsatellite primers developed from common carp （ Cyprinuscario ） for genomic analysis in mud carp （ Cirrhina molitorella ）, 24 primer pairs from common carp were designed to amplify microsatellite loci in the mud carp containing CA, GA, AT and GGGA sequences. Thirteen primers （54%） successfully amplified specific products in the mud carp and 11 primers （48%） showed high polymorphism in the mud carp population. The results indicated that the average number of alleles per locus in the mud carp stocks was 5.2. Average heterozygosity （Ho）, unbiased expected heterozygosity （He） and polymorphism information content （PIC） in the wild population were 0.61 ± 0.2, 0.8 ± 0.09 and 0.72 ± 0.1 respectively. Several Hardy-Weinberg departure value were significandy departed from Hardy-Weinberg equilibrium. The study showed that microsatellite primers from a species of Cyprinidae can be used for mud carp genetic analysis without much cost or time input.
基金Supported by National Natural Science Foundation of China(U1304324)~~
文摘The aim was to protect the Henan Yellow River carp germplasm resources and provide a scientific basis for the meat quality improvement of Yellow River carps. With artificially farmed and wild Henan Yellow River carps as the research objects, comparative study on muscle fiber diameter and density, routine nutritional composition, calcium and phosphorus contents and amino acids composition was conducted between the two groups of carps. The results showed that the moisture content was significantly higher （P 〈0.05）, the crude fat and crude protein contents were significantly lower （P〈0.05）, the essential amino acids and total amino acids contents were lower （P〉0.05）, the phosphorus content was higher （P 〉0.05）, and the calcium content was lower （P〉0.05） in the wild group compared with those in the farming group. The analysis of muscle fiber characteristics showed that there were significant differences in the average muscle fiber diameter （P〈0.05） and muscle fiber density （P〈0.01） between the two groups of Henan Yellow River carps.
基金Supported by Natural Science Foundation in Hunan Province(05JJ40035)~~
文摘[ Objective] The study aimed to discuss the toxicity effect of abamectin and ehlorpyrifos on grass carp （ Ctenopharyngdon idllus ） and their interrela- tions. [ Method] Taking healthy and active grass carp as the object, the acute toxicity test of single pesticide and the combined toxicity test of two pesticides on grass carp were carried out. [ Result] The LC50 of abamectin and chlorpyrifes and the mixture of two pesticides against grass carp at 24, 48, 72 and 96 h were as follows： abamectin： 0.54, 0.49, 0. 17 and 0.10 rag/L; chlorpyrifes ： 0.29, 0.21, 0.12 and 0.05 rag/L; the mixture of two pesticides ： 1.22, 1.08, 0.99 and 0. 86 mg/L. The safe concentration （SC） of ahamectin, chlorpyrifos and the mixture of two pesticides were 0.010, 0.005 and 0.086 rag/L, respectively. The tox- icity of the pesticides in sequence was ablorpyrifos 〉 ahamectin 〉 the mixture of two pesticides. [ Conclusion ] Different concentrations of abamectin, chlorpyrifos and the mixture of two pesticides had remarkable effect on the growth of grass carp, the higher the concentration was, the greater the toxicity effect was. Ahamectin at low toxicity intensity had certain relief function on the toxicity of ehlorpyrifos.
文摘During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp.
基金The Natural Science Project of Xichang College(xA0509)~~
文摘[Objective] The curative effect of onion on bacterial rotted gill disease in grass carp was researched [Method] The combination method of taking medicine through oral and spraying was used to cure sick grass carp for 1 period of treatment in room under artificial conditions. [Result] Different concentrations of onion generated different cure rates. When the combination was adding 1.0% - 2.0% medicine into feed and spraying 2.0 g,/m3 - 5.0 g,/m3, the curative result was the best with cure rate was 70% -90% [ Conclusion] The onion was effective on curing bacterial rotted gill disease in grass carp and could be taken as curative medicine.
基金Supported by Scientific Research Foundation of the Education Department of Hubei Province,China(Q20141701)~~
文摘With fresh-cut grass carp belly as the test material, the antibacterial con- ditions of a disinfection agent sodium hypochlorite were optimized. In addition, the shelf lives of various grass carp products were compared after disinfection, so as to provide certain basis for the processing of fresh-cut grass carp products. The re- sults showed that the optimal disinfection conditions of sodium hypochlorite were as follows： concentration 300 mg/L, soak time 5 min and solid-liquid ratio 1 g ： 5 ml. Under the optimal disinfection conditions, the inhibition rates of total bacteria and Pseudomonas reached 83% and 81%, respectively. The shelf life of refrigerated fresh-cut grass carp belly disinfected by sodium hypochlorite and packed in bag filled with gases could be extended as long as 11 d, which was increased by 5 d （83%） compared with that in the control group. The disinfection by sodium hypochlorite could significantly reduce the initial bacterial colony number and improve the sensory quality of fresh-cut grass carp belly, as well as extend the shelf life of refrigerated and modified gas-packed grass carp belly.
基金Supported by Open Fund of Key Laboratory of Ecological Environment and Resources of Inland Fisheries,Chinese Academy of Fishery SciencesThree Projects for Fishery of Jiangsu Province(K2008-3)~~
文摘[Objective] The aim was to discuss the morphological differences of four carp populations.[Method] Based on traditional morphological data（including countable and measurable characters） and framework data,the morphological differences and phylogenesis of Yellow River carp population（YR）,wild four-naris carp population（WF）,farmed four-naris carp population（FF） and carp population from Suzhou section of Wusong River（WR） were studied by means of multivariate analysis.[Result] There was no significant difference（P〉0.05） in countable characters among four carp populations.Discriminant analysis showed the discriminating accuracy of YR,WF,FF and WR population were 87.5%,78.1%,73.5% and 96.8% respectively,and the synthetic discriminating accuracy of four populations was up to 83.7%.In addition,cluster analysis revealed that YR population and WF population clustered together,while FF population and WR population clustered together.Principal component analysis indicated that the morphological differences among four populations mainly came from the head,tail and vertical axis of fish.[Conclusion] The study could provide theoretical foundations for the purification and rejuvenation of rare four-naris carp.
