Jarilla caudata Standl.(Caricaceae)is a wild herbaceous plant native to Mexico recognized for its edible fruits.It is considered to be the closest taxonomically species to Carica papaya L.(Caricaceae),whose seeds have...Jarilla caudata Standl.(Caricaceae)is a wild herbaceous plant native to Mexico recognized for its edible fruits.It is considered to be the closest taxonomically species to Carica papaya L.(Caricaceae),whose seeds have good nutritional and functional properties.This study analyzes and compares the seed chemical compositions of J.caudata and C.papaya to study the nutritional and functional potential of J.caudata seeds.The analysis of the proximate composition was based on standard methods.High-performance liquid chromatography was used to determine the free amino acid profile,gas chromatography to quantify the fatty acid content,and inductively coupled plasma–optical emission spectrometry to measure minerals.Both J.caudata and C.papaya seeds have high protein(24.03%to 26.94%)and lipid(21.32%to 25.07%)content.The mineral study indicated high potassium,calcium,iron,and zinc content.Minor functional compounds present,with similar contents in J.caudata and C.papaya seeds,were soluble sugars,phytic acid,polyphenols,and pectin.The main fatty acid in seed oil was oleic acid(C18:1),with 61.4%in J.caudata seeds and 72.6%in C.papaya seeds.Among free amino acids,leucine with 6.9/100 g free amino acids and phenylalanine with 13.6/100 g free amino acids were the most abundant in the seeds of J.caudata and C.papaya,respectively.Polyphenols of J.caudata and C.papaya seeds showed similar antioxidant activity.J.caudata seeds may represent a useful source of nutritional compounds for food and feeding,and functional compounds with antioxidant activity.展开更多
Objective: To characterize the types, contents, and peroxynitrite-scavenging activities of flavonoids in the leaf of Carica papaya(C. papaya).Methods: Chromatographic and spectroscopic techniques along with high perfo...Objective: To characterize the types, contents, and peroxynitrite-scavenging activities of flavonoids in the leaf of Carica papaya(C. papaya).Methods: Chromatographic and spectroscopic techniques along with high performance liquid chromatography quantitative analysis and peroxynitrite-scavenging assay were performed to isolate and quantify flavonoid compounds in the flavonoid-rich fraction(Bu OH fraction) derived from Me OH extract of C. papaya leaves and evaluate their peroxynitrite-scavenging activities.Results: Seven flavonoids were isolated from the leaves of C. papaya, including quercetin 3-(2~G-rhamnosylrutinoside), kaempferol 3-(2~G-rhamnosylrutinoside), quercetin 3-rutinoside, myricetin 3-rhamnoside, kaempferol 3-rutinoside, quercetin, and kaempferol. All of the substances exhibited potent activities on peroxynitrite scavenging(IC50 4.15 mmol/L), which were stronger than the positive control, L-penicillamine(6.90 mmol/L). The content of kaempferol 3-(2~G-rhamnosylrutinoside) was significantly higher than other identified compounds(123.18 mg/g Bu OH fraction and 7.23 mg/g Me OH extract).Conclusions: The results of the present study demonstrate the potent antioxidant flavonoids of C. papaya leaf, with kaempferol 3-(2~G-rhamnosylrutinoside) as the major one.展开更多
基金financed with an I-COOP project(ref.COOPA 20469)funded by C.S.I.C.To National Council Humanities for Science and Technology(CONAHCYT,Mexico)by the postgraduate scholarship granted to Mario Felipe Gonzalez Gonzalez(No.001073088)。
文摘Jarilla caudata Standl.(Caricaceae)is a wild herbaceous plant native to Mexico recognized for its edible fruits.It is considered to be the closest taxonomically species to Carica papaya L.(Caricaceae),whose seeds have good nutritional and functional properties.This study analyzes and compares the seed chemical compositions of J.caudata and C.papaya to study the nutritional and functional potential of J.caudata seeds.The analysis of the proximate composition was based on standard methods.High-performance liquid chromatography was used to determine the free amino acid profile,gas chromatography to quantify the fatty acid content,and inductively coupled plasma–optical emission spectrometry to measure minerals.Both J.caudata and C.papaya seeds have high protein(24.03%to 26.94%)and lipid(21.32%to 25.07%)content.The mineral study indicated high potassium,calcium,iron,and zinc content.Minor functional compounds present,with similar contents in J.caudata and C.papaya seeds,were soluble sugars,phytic acid,polyphenols,and pectin.The main fatty acid in seed oil was oleic acid(C18:1),with 61.4%in J.caudata seeds and 72.6%in C.papaya seeds.Among free amino acids,leucine with 6.9/100 g free amino acids and phenylalanine with 13.6/100 g free amino acids were the most abundant in the seeds of J.caudata and C.papaya,respectively.Polyphenols of J.caudata and C.papaya seeds showed similar antioxidant activity.J.caudata seeds may represent a useful source of nutritional compounds for food and feeding,and functional compounds with antioxidant activity.
基金Supported by the National Competitive Research Grant funded by the Ministry of Research,Technology and Higher Education,Republic of Indonesia(Grant No.056/UN8.2/PL/2015)
文摘Objective: To characterize the types, contents, and peroxynitrite-scavenging activities of flavonoids in the leaf of Carica papaya(C. papaya).Methods: Chromatographic and spectroscopic techniques along with high performance liquid chromatography quantitative analysis and peroxynitrite-scavenging assay were performed to isolate and quantify flavonoid compounds in the flavonoid-rich fraction(Bu OH fraction) derived from Me OH extract of C. papaya leaves and evaluate their peroxynitrite-scavenging activities.Results: Seven flavonoids were isolated from the leaves of C. papaya, including quercetin 3-(2~G-rhamnosylrutinoside), kaempferol 3-(2~G-rhamnosylrutinoside), quercetin 3-rutinoside, myricetin 3-rhamnoside, kaempferol 3-rutinoside, quercetin, and kaempferol. All of the substances exhibited potent activities on peroxynitrite scavenging(IC50 4.15 mmol/L), which were stronger than the positive control, L-penicillamine(6.90 mmol/L). The content of kaempferol 3-(2~G-rhamnosylrutinoside) was significantly higher than other identified compounds(123.18 mg/g Bu OH fraction and 7.23 mg/g Me OH extract).Conclusions: The results of the present study demonstrate the potent antioxidant flavonoids of C. papaya leaf, with kaempferol 3-(2~G-rhamnosylrutinoside) as the major one.
