BACKGROUND Overnutrition and unhealthy dietary habits are established risk factors for cardiovascular and renal diseases,which may lead to the development of cardiorenal syndrome(CRS).AIM To evaluate the cardiorenal p...BACKGROUND Overnutrition and unhealthy dietary habits are established risk factors for cardiovascular and renal diseases,which may lead to the development of cardiorenal syndrome(CRS).AIM To evaluate the cardiorenal protective potential of crude ethanol extract(CEE)of green cardamom(Elettaria cardamomum L.,Family Zingiberaceae).METHODS Rats were fed a high-fat diet to induce dyslipidemia and subsequently administered cisplatin(7.5 mg/kg)to induce CRS.CEE was administered orally to CRS rats at low(100 mg/kg)and high(200 mg/kg)doses for one month.Oxidative stress,inflammatory markers,cardiovascular disease markers,cardiac indices,and renal function(in plasma and urine)were assessed.The antioxidant activity and phenolic compound profile of CEE were evaluated.Additionally,the potential interactions of CEE phenolics with components of the Hippo signaling pathway(mammalian sterile 20-like kinase 1,large tumor suppressor kinase 1,Yes-associated protein,and transcriptional coactivator with PDZ-binding motif)were investigated using molecular docking.RESULTS Cisplatin administration combined with a high-fat diet effectively induced CRS,as evidenced by elevated oxidative stress,inflammation,and impaired cardiorenal parameters.Treatment with CEE at both doses improved these parameters,with the high dose demonstrating greater efficacy.CEE exhibited significant DPPH radical scavenging activity.Rosmarinic acid and gallic acid were identified as the major phenolic constituents.Molecular docking revealed strong binding affinities of rosmarinic acid and rutin with targets in the Hippo signaling pathway.CONCLUSION These findings demonstrate the cardioprotective and renoprotective potential of CEE as a phenolic-rich dietary supplement.CEE mitigated inflammation and oxidative stress,key contributors to CRS pathogenesis.Furthermore,molecular docking suggests that the phenolic compounds in CEE may exert protective effects by modulating the Hippo signaling pathway.Overall,CEE shows promise as a natural therapeutic agent for the prevention and/or management of cardiorenal syndrome.展开更多
Objective:To study the potential ameliorating properties of cardamom Elettaria cardamomum (E.cardamomum) L.Maton against pan masala induced damage in lung of male Swiss mice. Methods:The experimental animals were divi...Objective:To study the potential ameliorating properties of cardamom Elettaria cardamomum (E.cardamomum) L.Maton against pan masala induced damage in lung of male Swiss mice. Methods:The experimental animals were divided into 3 groups(control,pan masala treated group and pan masala with cardamom treated group) to evaluate pan masala toxicity.The observations were substantiated with profound changes in the lung tissue as revealed in the histologic and transmission electron microscopic examinations.Results:Lung of pan masala treated group showed adenocarcinoma,edema,and inflammation with increased activity of acid phosphatase,alkaline phosphatase,and lactate dehydrogenase.The deleterious effects were seen to be less in cardamom treated group and the enzymatic activity also decreased significantly (P【0.05)\ in the ameliorating group.Conclusions:Thus,the present experiment exciting results are observed when cardamom is supplemented with pan masala,or when given alone.展开更多
Cinnamon, cardamom and cloves have been widely used for medicinal purposes as well as essential cooking ingredients for flavor. The objective of the research was to investigate the antioxidant content, antioxidant cap...Cinnamon, cardamom and cloves have been widely used for medicinal purposes as well as essential cooking ingredients for flavor. The objective of the research was to investigate the antioxidant content, antioxidant capacity, and inhibition of lipid and carbohydrate metabolizing enzyme activities of selected spices (cinnamon, cardamom & cloves) methanol (ME) and water extracts (WE). The phytochemical content was determined by total phenolic and total flavanoid content. The antioxidant potential was determined by measuring 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and Ferric Reducing Antioxidant Power (FRAP) in spice’s (ME) and (WE) extracts. Total phenolic (GAE mg/100g dry weight) and flavonoid (mg CE/100g dry weight) content were the highest in Cloves (ME) 174.4 and 101.06. The lowest values for phenolic content were seen in ME and WE of Cardamom at 31.24 and 7.55. The DPPH IC50 values ranged from 0.22mg/mL (Cloves ME) to 0.60mg/mL (Cardamom ME). FRAP (μmol Fe2+/100g dry weight) for Cinnamon, Cardamom (ME) was 2438.5 and 325. Clove (ME) had a significantly higher reducing potential of 6888.5 which might have been attributed by the high amounts of phenolics and flavonoids in the spice. FRAP in spice extracts (WE) was lower with values of 2296.5 and 218.5 and 2310.5, respectively. The highest inhibition of the α-glucosidase was observed by Clove (ME) 86.5%, which also had the highest amylase enzyme inhibition at 71%. However, inhibition of the lipase enzyme was highest by the Cinnamon (WE) extracts 44.3%. The potential of phytochemicals in spices was studied and if consumed in high amounts could offer antioxidative properties and regulate key digestive enzymes which may lead to prevention or decreased progression of diseases such as Cancer, Diabetes and Cardiovascular diseases.展开更多
Cultivation of cash crops, such as cardamom (Elettaria cardamomum) in the forest understorey is a common practice in many tropical forests. Over time, cultivation may change forest structure and species composition, l...Cultivation of cash crops, such as cardamom (Elettaria cardamomum) in the forest understorey is a common practice in many tropical forests. Over time, cultivation may change forest structure and species composition, leading to gradual degradation of biodiversity and ecosystem services. Effective conservation of these forests requires an enhanced understanding of the demographic processes such as soil seed bank that may greatly influence future forest composition. We examined how the soil seed bank structure and composition responds to cardamom cultivation in a high conservation value Sri Lankan montane rain forest. Soil samples from natural forest with abandoned cardamom plantations (CP) and adjacent natural forest (NF) patches without cardamom were collected in dry and wet seasons. Soil samples were spread out in trays in a shade house and germination was recorded weekly for 19 weeks. The density of seeds in the soil seed bank was much higher in CP than NF. While grasses and forbs contributed the highest number and percentage of seeds in soils of both forest types, their densities in the soil seed bank were 9 and 2 times greater in the CP than the NF, respectively. Seeds of the non-native herbs Ageratina riparia and E. cardamomum were 4 and 20 times greater in the soil of CP, respectively. Seeds of light demanding tree species such as Macaranga indica were restricted to soils of CPs. Overstorey tree community of each forest type was poorly represented in their respective soil seed banks. The high density of seeds of pioneer trees and non-native herbs in the soil of CPs, combined with higher light transmission to the ground floor may exacerbate competition for resources with the seedlings of late successional trees of high conservation value. To overcome this barrier and enhance conservation value of the forest, restoration strategies may need to focus on transplanting seedlings of these species into forest with abandoned cardamom plantations.展开更多
Cardamom oil is one of the active herbs used as a treatment in different diseases by the function of the blood serum components.Among the methods used for identification of function group which acted in the human body...Cardamom oil is one of the active herbs used as a treatment in different diseases by the function of the blood serum components.Among the methods used for identification of function group which acted in the human body,the method of electrochemical analysis using cyclic voltammetry was used to determine the effectiveness of cardamom by the oxidation-reduction reaction in the blood medium.Results showed that the cardamom compound presented peaks of oxidation-reduction in the blood serum medium of 0.8 and-0.65 V,respectively.The oxidation peak disappeared in alkaline pH;hence,the cardamom acted as anti-oxidative compound in alkaline medium and with ascorbic acid.But the oxidation peak was enhanced in acidic pH.Other electrochemical parameters of cardamom in blood serum medium were determined,including studies on different scan rates,reliability and stability.展开更多
The study offers a conceptual framework illuminating sustainable rural development by a continuous cycling interplay among five interdependent systems,physical,social,economic,knowledge,and creative.The framework is a...The study offers a conceptual framework illuminating sustainable rural development by a continuous cycling interplay among five interdependent systems,physical,social,economic,knowledge,and creative.The framework is applied to analyze the livelihood transition in the economic system and conversion of traditional knowledge in the creative system for farm households engaged in large cardamom cultivation in Northern mountainous region in Vietnam.This study used a probit model to examine a data set comprising 300 households and confirmed that factors within the social and physical systems(such as labor,access to information,social networks,land use,and transportation infrastructure)determined livelihood transition to tourism employment within the economic system.In addition,the conversion of traditional knowledge in the creative system was found to be contingent upon non-farm employment and credit capital in the economic system,as well as traditional knowledge and knowledge exchange in the knowledge system.This study demonstrates how the sustainable development of rural agriculture can be achieved by converting traditional knowledge and transferring livelihood,contributing to attain Sustainable Development Goals 2(Zero Hunger)and SDG 12(Responsible Consumption and Production).展开更多
Extraction of quality RNA for molecular biology applications from perennial woody plants like mulberry is complicated due to the presence of high polysaccharides, polyphenols and other secondary metabolites. Since the...Extraction of quality RNA for molecular biology applications from perennial woody plants like mulberry is complicated due to the presence of high polysaccharides, polyphenols and other secondary metabolites. Since the existing methods failed to yield quality RNA in sufficient quantity from leaf and root tissues of mulberry, in this study, we modified the CTAB-based protocol. The standardised protocol yielded high quantity (520.00 μg/g fresh weight of leaf tissue) of quality RNA and the RNA extracted was suitable for all downstream applications such as cDNA synthesis, PCR and whole transcriptome analysis. The method developed was also found to be useful for isolating good quality and quantity total RNA from desiccated and salinity stressed leaf tissues of mulberry. The protocol was also applied successfully to isolate total RNA from leaf tissues of other species such as cardamom, papaya and rice.展开更多
The value-chain approach(VCA)was used for exploring how a niche crop,namely large cardamom(Amomum subulatum Roxb.),can be developed sustainably to increase incomes and enhance rural livelihoods in the mountains.Large ...The value-chain approach(VCA)was used for exploring how a niche crop,namely large cardamom(Amomum subulatum Roxb.),can be developed sustainably to increase incomes and enhance rural livelihoods in the mountains.Large cardamom is a high-value,agro-climatically suitable,and non-perishable spice crop grown in the Himalayan region.Originating in Sikkim,the crop is important to the local economy and is sold in both domestic and international markets;however,its production in India has been declining significantly in recent years.Using VCA and participatory methods of data collection the study helped elicit value chain actors’perspectives on various reasons for the decline as well as potential strategies to improve the cardamom value chain.The results present the multiple environmental and climatic(e.g.water stress),social(e.g.women participation)and institutional(e.g.lack of trust between actors)challenges and corresponding upgrading strategies.Through participatory multi-criteria analysis(MCA),the strategies were prioritized based on the overall preferences of multiple stakeholders.The analysis considered eight criteria and 25 options,or strategies,and found that that marketability and profitability were the most important criteria,and increasing cardamom production,the most important optiontrait.Stakeholder discussions along with sensitivity analysis also showed how these options could be implemented and revealed how criteria preferences can change with expert inputs.This combination of VCA and MCA,because it supports participatory decision-making and balances multiple dimensions of sustainable development,can be effectively deployed in planning for agricultural development in the mountains.展开更多
基金Supported by the National Research Centre,Egypt,No.13050203.
文摘BACKGROUND Overnutrition and unhealthy dietary habits are established risk factors for cardiovascular and renal diseases,which may lead to the development of cardiorenal syndrome(CRS).AIM To evaluate the cardiorenal protective potential of crude ethanol extract(CEE)of green cardamom(Elettaria cardamomum L.,Family Zingiberaceae).METHODS Rats were fed a high-fat diet to induce dyslipidemia and subsequently administered cisplatin(7.5 mg/kg)to induce CRS.CEE was administered orally to CRS rats at low(100 mg/kg)and high(200 mg/kg)doses for one month.Oxidative stress,inflammatory markers,cardiovascular disease markers,cardiac indices,and renal function(in plasma and urine)were assessed.The antioxidant activity and phenolic compound profile of CEE were evaluated.Additionally,the potential interactions of CEE phenolics with components of the Hippo signaling pathway(mammalian sterile 20-like kinase 1,large tumor suppressor kinase 1,Yes-associated protein,and transcriptional coactivator with PDZ-binding motif)were investigated using molecular docking.RESULTS Cisplatin administration combined with a high-fat diet effectively induced CRS,as evidenced by elevated oxidative stress,inflammation,and impaired cardiorenal parameters.Treatment with CEE at both doses improved these parameters,with the high dose demonstrating greater efficacy.CEE exhibited significant DPPH radical scavenging activity.Rosmarinic acid and gallic acid were identified as the major phenolic constituents.Molecular docking revealed strong binding affinities of rosmarinic acid and rutin with targets in the Hippo signaling pathway.CONCLUSION These findings demonstrate the cardioprotective and renoprotective potential of CEE as a phenolic-rich dietary supplement.CEE mitigated inflammation and oxidative stress,key contributors to CRS pathogenesis.Furthermore,molecular docking suggests that the phenolic compounds in CEE may exert protective effects by modulating the Hippo signaling pathway.Overall,CEE shows promise as a natural therapeutic agent for the prevention and/or management of cardiorenal syndrome.
文摘Objective:To study the potential ameliorating properties of cardamom Elettaria cardamomum (E.cardamomum) L.Maton against pan masala induced damage in lung of male Swiss mice. Methods:The experimental animals were divided into 3 groups(control,pan masala treated group and pan masala with cardamom treated group) to evaluate pan masala toxicity.The observations were substantiated with profound changes in the lung tissue as revealed in the histologic and transmission electron microscopic examinations.Results:Lung of pan masala treated group showed adenocarcinoma,edema,and inflammation with increased activity of acid phosphatase,alkaline phosphatase,and lactate dehydrogenase.The deleterious effects were seen to be less in cardamom treated group and the enzymatic activity also decreased significantly (P【0.05)\ in the ameliorating group.Conclusions:Thus,the present experiment exciting results are observed when cardamom is supplemented with pan masala,or when given alone.
文摘Cinnamon, cardamom and cloves have been widely used for medicinal purposes as well as essential cooking ingredients for flavor. The objective of the research was to investigate the antioxidant content, antioxidant capacity, and inhibition of lipid and carbohydrate metabolizing enzyme activities of selected spices (cinnamon, cardamom & cloves) methanol (ME) and water extracts (WE). The phytochemical content was determined by total phenolic and total flavanoid content. The antioxidant potential was determined by measuring 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and Ferric Reducing Antioxidant Power (FRAP) in spice’s (ME) and (WE) extracts. Total phenolic (GAE mg/100g dry weight) and flavonoid (mg CE/100g dry weight) content were the highest in Cloves (ME) 174.4 and 101.06. The lowest values for phenolic content were seen in ME and WE of Cardamom at 31.24 and 7.55. The DPPH IC50 values ranged from 0.22mg/mL (Cloves ME) to 0.60mg/mL (Cardamom ME). FRAP (μmol Fe2+/100g dry weight) for Cinnamon, Cardamom (ME) was 2438.5 and 325. Clove (ME) had a significantly higher reducing potential of 6888.5 which might have been attributed by the high amounts of phenolics and flavonoids in the spice. FRAP in spice extracts (WE) was lower with values of 2296.5 and 218.5 and 2310.5, respectively. The highest inhibition of the α-glucosidase was observed by Clove (ME) 86.5%, which also had the highest amylase enzyme inhibition at 71%. However, inhibition of the lipase enzyme was highest by the Cinnamon (WE) extracts 44.3%. The potential of phytochemicals in spices was studied and if consumed in high amounts could offer antioxidative properties and regulate key digestive enzymes which may lead to prevention or decreased progression of diseases such as Cancer, Diabetes and Cardiovascular diseases.
文摘Cultivation of cash crops, such as cardamom (Elettaria cardamomum) in the forest understorey is a common practice in many tropical forests. Over time, cultivation may change forest structure and species composition, leading to gradual degradation of biodiversity and ecosystem services. Effective conservation of these forests requires an enhanced understanding of the demographic processes such as soil seed bank that may greatly influence future forest composition. We examined how the soil seed bank structure and composition responds to cardamom cultivation in a high conservation value Sri Lankan montane rain forest. Soil samples from natural forest with abandoned cardamom plantations (CP) and adjacent natural forest (NF) patches without cardamom were collected in dry and wet seasons. Soil samples were spread out in trays in a shade house and germination was recorded weekly for 19 weeks. The density of seeds in the soil seed bank was much higher in CP than NF. While grasses and forbs contributed the highest number and percentage of seeds in soils of both forest types, their densities in the soil seed bank were 9 and 2 times greater in the CP than the NF, respectively. Seeds of the non-native herbs Ageratina riparia and E. cardamomum were 4 and 20 times greater in the soil of CP, respectively. Seeds of light demanding tree species such as Macaranga indica were restricted to soils of CPs. Overstorey tree community of each forest type was poorly represented in their respective soil seed banks. The high density of seeds of pioneer trees and non-native herbs in the soil of CPs, combined with higher light transmission to the ground floor may exacerbate competition for resources with the seedlings of late successional trees of high conservation value. To overcome this barrier and enhance conservation value of the forest, restoration strategies may need to focus on transplanting seedlings of these species into forest with abandoned cardamom plantations.
文摘Cardamom oil is one of the active herbs used as a treatment in different diseases by the function of the blood serum components.Among the methods used for identification of function group which acted in the human body,the method of electrochemical analysis using cyclic voltammetry was used to determine the effectiveness of cardamom by the oxidation-reduction reaction in the blood medium.Results showed that the cardamom compound presented peaks of oxidation-reduction in the blood serum medium of 0.8 and-0.65 V,respectively.The oxidation peak disappeared in alkaline pH;hence,the cardamom acted as anti-oxidative compound in alkaline medium and with ascorbic acid.But the oxidation peak was enhanced in acidic pH.Other electrochemical parameters of cardamom in blood serum medium were determined,including studies on different scan rates,reliability and stability.
文摘The study offers a conceptual framework illuminating sustainable rural development by a continuous cycling interplay among five interdependent systems,physical,social,economic,knowledge,and creative.The framework is applied to analyze the livelihood transition in the economic system and conversion of traditional knowledge in the creative system for farm households engaged in large cardamom cultivation in Northern mountainous region in Vietnam.This study used a probit model to examine a data set comprising 300 households and confirmed that factors within the social and physical systems(such as labor,access to information,social networks,land use,and transportation infrastructure)determined livelihood transition to tourism employment within the economic system.In addition,the conversion of traditional knowledge in the creative system was found to be contingent upon non-farm employment and credit capital in the economic system,as well as traditional knowledge and knowledge exchange in the knowledge system.This study demonstrates how the sustainable development of rural agriculture can be achieved by converting traditional knowledge and transferring livelihood,contributing to attain Sustainable Development Goals 2(Zero Hunger)and SDG 12(Responsible Consumption and Production).
文摘Extraction of quality RNA for molecular biology applications from perennial woody plants like mulberry is complicated due to the presence of high polysaccharides, polyphenols and other secondary metabolites. Since the existing methods failed to yield quality RNA in sufficient quantity from leaf and root tissues of mulberry, in this study, we modified the CTAB-based protocol. The standardised protocol yielded high quantity (520.00 μg/g fresh weight of leaf tissue) of quality RNA and the RNA extracted was suitable for all downstream applications such as cDNA synthesis, PCR and whole transcriptome analysis. The method developed was also found to be useful for isolating good quality and quantity total RNA from desiccated and salinity stressed leaf tissues of mulberry. The protocol was also applied successfully to isolate total RNA from leaf tissues of other species such as cardamom, papaya and rice.
基金funding support from Himalayan Adaptation, Water and Resilience (HI-AWARE) Research Consortium
文摘The value-chain approach(VCA)was used for exploring how a niche crop,namely large cardamom(Amomum subulatum Roxb.),can be developed sustainably to increase incomes and enhance rural livelihoods in the mountains.Large cardamom is a high-value,agro-climatically suitable,and non-perishable spice crop grown in the Himalayan region.Originating in Sikkim,the crop is important to the local economy and is sold in both domestic and international markets;however,its production in India has been declining significantly in recent years.Using VCA and participatory methods of data collection the study helped elicit value chain actors’perspectives on various reasons for the decline as well as potential strategies to improve the cardamom value chain.The results present the multiple environmental and climatic(e.g.water stress),social(e.g.women participation)and institutional(e.g.lack of trust between actors)challenges and corresponding upgrading strategies.Through participatory multi-criteria analysis(MCA),the strategies were prioritized based on the overall preferences of multiple stakeholders.The analysis considered eight criteria and 25 options,or strategies,and found that that marketability and profitability were the most important criteria,and increasing cardamom production,the most important optiontrait.Stakeholder discussions along with sensitivity analysis also showed how these options could be implemented and revealed how criteria preferences can change with expert inputs.This combination of VCA and MCA,because it supports participatory decision-making and balances multiple dimensions of sustainable development,can be effectively deployed in planning for agricultural development in the mountains.
