目的评估碳青霉烯类抑制法(carbapenem inactivation method,CIM)和Carba NP对铜绿假单胞菌碳青霉烯酶表型的筛选能力。方法选取河北省医学微生物菌种保藏库(Hebei Provincial Bank for Medical Culture Collections,HBMCC)保存的碳青...目的评估碳青霉烯类抑制法(carbapenem inactivation method,CIM)和Carba NP对铜绿假单胞菌碳青霉烯酶表型的筛选能力。方法选取河北省医学微生物菌种保藏库(Hebei Provincial Bank for Medical Culture Collections,HBMCC)保存的碳青霉烯类耐药铜绿假单胞菌146株,分别用CIM和Carba NP法检测碳青霉烯酶活性,采用PCR方法检测VIM-1、KPC-2、IMP-4、NDM-1基因。结果 146株铜绿假单胞菌中有11株Carba NP阳性,阳性率为7.5%(11/146);13株CIM阳性,阳性率为8.9%(13/146)。9株碳青霉烯酶编码基因阳性,其中6株VIM-1阳性,1株VIM-1和IMP-4同时阳性,2株KPC-2阳性;碳青霉烯酶编码基因阳性的菌株Carba NP和CIM均阳性;5株基因检测阴性菌株中1株仅Carba NP阳性,3株仅CIM阳性,1株Carba NP和CIM均阳性。结论 CIM能够准确快速筛选铜绿假单胞菌碳青霉烯酶活性,是一种经济高效的表型筛选方法。展开更多
After the persistent practice in the 1990's,using "Open System" idea to integrate large scale computer systems has been the consenting fact. The new mechanism based on mobile agent is discussed and the c...After the persistent practice in the 1990's,using "Open System" idea to integrate large scale computer systems has been the consenting fact. The new mechanism based on mobile agent is discussed and the core architecture called common agent request broker architecture (CARBA)is established to embody intelligent collaboration in the next generation open system. In order to evaluate whether CARBA is open system,ODP specification is used to validate the openness of CARBA.展开更多
文摘After the persistent practice in the 1990's,using "Open System" idea to integrate large scale computer systems has been the consenting fact. The new mechanism based on mobile agent is discussed and the core architecture called common agent request broker architecture (CARBA)is established to embody intelligent collaboration in the next generation open system. In order to evaluate whether CARBA is open system,ODP specification is used to validate the openness of CARBA.
