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Distinguishing between wild-caught and captive-bred Common Pheasantusing methylation rate of skeletal muscle DNA
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作者 Wenhui Wang Lijun Lin +15 位作者 Yue Ma Yan Cui Qi Zhang Jincheng Yang Yongheng Zhou Liangyu Cui Boyang Liu Chang Su Mengjia Yu Yuwei Gao Peng Gao Yujia Du Yu Zhou Elizabeth Kamili Shuhui Yang Yanchun Xu 《Avian Research》 2025年第2期264-274,共11页
Illegal hunting and trafficking of wildlife and their derivatives extort unprecedented population decline of relatively many species pushing them towards extinction.Notwithstanding contemporary counteracting intervent... Illegal hunting and trafficking of wildlife and their derivatives extort unprecedented population decline of relatively many species pushing them towards extinction.Notwithstanding contemporary counteracting interventions at international,regional,national and local levels,wildlife farming is advocated as an alternative approach to minimize pressure on wild populations.For wildlife farming to be an effective conservation tool,the integration of wildlife forensics is inevitable to allow distinction between captive-bred and wild-caught species.To this end,we analyzed methylation rates of skeletal muscle samples(pectoralis major,triceps brachii,gastrocnemius,biceps femoris,and neck muscles)from 60 captive-bred and 30 wild-caught Common Pheasant.A total of 13,507 differentially methylated regions were identified between five wild-caught and five captive-bred individuals through whole-genome methylation sequencing(WGBS).Based on the selected five methylation sites,LOC116231076,LOC116242223,ATAD2B,EGFL6,and HS2ST,quantitative detection technique was developed using methylation-sensitive high-resolution melting curve(MS-HRM)to measure methylation rates.The results showed significant differences in methylation rates at all differential sites between wild-caught and captive-bred individuals(|t|=0.67–33.10,P=0.000–0.042).The discrimination accuracy rate of each locus was highest in the gastrocnemius muscle and lowest in the neck muscle.The discrimination accuracy rate on LOC116231076,LOC116242223,ATAD2B,EGFL6,and HS2ST methylation sites for gastrocnemius muscle was 64.98%,100.00%,68.54%,63.79%,and 63.70%,respectively;and for neck muscle it was 67.42%,68.06%,83.61%,65.04%,and68.85%,respectively.The united discrimination accuracy rate of the five loci were 100.00%for gastrocnemius muscle,99.78%for biceps femoris muscle,97.52%for pectoralis major muscle,93.96%for triceps brachii muscle,and 91.63%for neck muscle,respectively.The panel also revealed excellent repeatability,reproducibility,sensitivity and universality to mammals and avian species.This study establishes an effective,accurate and low-cost identification technology for the identification of wild and farmed Common Pheasant,and also provides a reference for the development of identification methods for other species. 展开更多
关键词 capTIVE common Pheasant DNA methylation Methylation-sensitive high-resolution melting curve Source identification WILD
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猪圆环病毒4型Cap蛋白的原核表达及多克隆抗体制备
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作者 文英会 崔鸿博 +5 位作者 陈曦艋 陈红英 李金磊 赵丽 刘占通 闫志浩 《中国畜牧兽医》 北大核心 2025年第10期4904-4911,共8页
【目的】通过原核表达系统表达猪圆环病毒4型(Porcine circovirus type 4,PCV4)衣壳蛋白(Cap),并制备其多克隆抗体,为PCV4的防控提供技术支撑。【方法】基于PCV4开放阅读框2(ORF2)基因的免疫原区域设计1对特异性引物,通过PCR扩增获得PCV... 【目的】通过原核表达系统表达猪圆环病毒4型(Porcine circovirus type 4,PCV4)衣壳蛋白(Cap),并制备其多克隆抗体,为PCV4的防控提供技术支撑。【方法】基于PCV4开放阅读框2(ORF2)基因的免疫原区域设计1对特异性引物,通过PCR扩增获得PCV4 ORF2基因序列,并将其连接至pET-28a(+)载体,构建重组表达质粒pET-28a-ORF2;将经PCR和测序鉴定正确的重组质粒pET-28a-ORF2转化大肠杆菌BL21(DE3)感受态细胞,经1 mmol/L IPTG诱导表达重组Cap蛋白,利用SDS-PAGE分析诱导的重组蛋白产物。利用尿素纯化重组Cap蛋白并通过Western blotting鉴定;将纯化的重组Cap蛋白免疫新西兰大白兔制备多克隆抗体并进行Western blotting鉴定,应用间接ELISA法检测兔血清抗体效价。【结果】PCR扩增出大小为414 bp的PCV4 ORF 2基因片段,成功构建重组表达质粒pET-28a-ORF2。SDS-PAGE分析结果显示,诱导表达的重组Cap蛋白大小为16 ku;Western blotting结果表明制备的兔源多克隆抗体具有良好的免疫原性。间接ELISA检测显示,制备的兔血清抗体效价达1∶12800。【结论】本研究利用原核表达系统成功表达了PCV4 Cap蛋白并制备了多克隆抗体,试验结果为PCV4 Cap蛋白的深入研究以及后续开发PCV4基因工程亚单位疫苗、血清学诊断试剂等提供了物质基础。 展开更多
关键词 猪圆环病毒4型(PCV4) cap蛋白 原核表达 多克隆抗体
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基于北斗与CAP协议的高精度滑坡预警信息发布技术研究
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作者 黄建华 田爱国 +2 位作者 张姚 樊晶晶 官明霖 《信息产业报道》 2025年第4期0059-0061,共3页
为了将高精度滑坡预警信息实时发往灾区,降低滑坡对人员所造成的生命和财产损失,设计了一种基于北斗短报文通信方式发布高精度滑坡预警信息的方法。优化了滑坡预警信息的发布方式,将标准的北斗短报文协议和 CAP(Common Alerting Protoc... 为了将高精度滑坡预警信息实时发往灾区,降低滑坡对人员所造成的生命和财产损失,设计了一种基于北斗短报文通信方式发布高精度滑坡预警信息的方法。优化了滑坡预警信息的发布方式,将标准的北斗短报文协议和 CAP(Common Alerting Protocol)通用警报协议相结合,丰富和完善了预警信息的内容,对高精度滑坡预警信息进行了封装传输,并通过北斗终端自动回执北斗短报文预警消息,实现了基于互联网和北斗短报文通信的信息互联互通,为预警发布方式提供了应急备用手段,提高了滑坡预警信息发布的时效性和可靠性。 展开更多
关键词 滑坡 高精度 短报文协议 cap协议 预警信息发布
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表达GoAstV-2 Cap蛋白的重组FAdV-4的构建与鉴定 被引量:1
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作者 李星雨 李岩 +14 位作者 杨盼盼 刘俊杰 相梦佳 朱玉涛 邱路遥 乔麒龙 张伯顺 卜德新 韩城昊 于春梅 丛雁方 王增 李建丽 王白玉 赵军 《中国兽医学报》 北大核心 2025年第3期443-448,513,共7页
为构建表达鹅星状病毒基因2型(GoAstV-2)衣壳蛋白(Cap)的重组禽腺病毒4型(FAdV-4),本研究将GoAstV-2的Cap基因表达盒插入到含有FAdV-4感染性克隆p15A-cm-FAdV4-HNJZ中FAdV4基因组的1966 bp自然缺失区,将获得的重组感染性克隆p15A-cm-FAd... 为构建表达鹅星状病毒基因2型(GoAstV-2)衣壳蛋白(Cap)的重组禽腺病毒4型(FAdV-4),本研究将GoAstV-2的Cap基因表达盒插入到含有FAdV-4感染性克隆p15A-cm-FAdV4-HNJZ中FAdV4基因组的1966 bp自然缺失区,将获得的重组感染性克隆p15A-cm-FAdV4-HNJZ-Cap/GoAstV-2利用限制性内切酶线性化后转染鸡肝癌细胞系(chicken hepatoma cell line,LMH),拯救出表达Cap蛋白的重组病毒rFAdV4-Cap/GoAstV-2。将重组病毒在LMH细胞中连续传至第15代,提取重组病毒基因组DNA,用插入位点两侧的鉴定引物进行PCR和利用抗Cap蛋白多克隆抗体进行间接免疫荧光试验和Western blot对重组病毒进行鉴定,同时对重组病毒在LMH细胞中的复制动态进行了研究。结果显示,GoAstV-2的Cap基因能够稳定存在于重组病毒rFAdV4-Cap/GoAstV-2基因组中,而且获得稳定表达;重组病毒具有良好的体外复制能力。本研究所制备的重组病毒rFAdV4-Cap/GoAstV-2为研制防控鹅FAdV-4和GoAstV-2混合感染的新型高效二联灭活疫苗奠定了基础。 展开更多
关键词 鹅星状病毒基因2型 禽病毒4型 cap蛋白 二联灭活疫苗
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吉林省PCV3野毒株遗传进化分析及其Cap蛋白对巨噬细胞自噬的影响
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作者 闫佳慧 韩建男 +3 位作者 孙浩 李文龙 苗丽娟 刘东旭 《中国动物检疫》 2025年第2期114-119,共6页
为了探究吉林省PCV3野毒株的起源及其衣壳蛋白(Cap蛋白)是否可以引起巨噬细胞自噬,对10株吉林省PCV3野毒株(JL-1~10)全基因组序列进行遗传进化分析,并通过Western blot和qPCR检测Cap蛋白转染巨噬细胞后其自噬相关基因和蛋白表达量的变... 为了探究吉林省PCV3野毒株的起源及其衣壳蛋白(Cap蛋白)是否可以引起巨噬细胞自噬,对10株吉林省PCV3野毒株(JL-1~10)全基因组序列进行遗传进化分析,并通过Western blot和qPCR检测Cap蛋白转染巨噬细胞后其自噬相关基因和蛋白表达量的变化。结果显示:所有PCV3参考毒株与2018年在日本发现的蝙蝠圆环病毒株LC456718均在524~598 nt位置发生重组,但在JL-1~10中并未观察到该重组现象;JL-1~10与其他宿主来源圆环病毒在不同的分支中聚集;ORF1与其他圆环病毒基因组表现出明显的保守性,但ORF2没有表现出明显的保守性;JL-10和JL-5最近的共同祖先株起源时间为1978年,其他8株的共同祖先株起源时间为1979年;Cap蛋白转染巨噬细胞后,LC3-Ⅱ表达量增加,p62表达量减少;Beclin-1和LC3蛋白基因转录水平极显著升高(P<0.01),Atg7的转录水平显著升高(P<0.05)。结果表明:10株吉林省PCV3野毒株大约起源于40年前(依据参考毒株年份推断),与其他宿主来源圆环病毒的起源不同;Cap蛋白可以促进巨噬细胞自噬,引起自噬相关基因转录水平的升高。 展开更多
关键词 PCV3 进化分析 自噬 cap蛋白
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Clinical Effect of Yinhuang Qingfei Capsules in Treatment of Asymptomatic and Mild/Common Severe Acute Respiratory Syndrome Coronavirus 2 Infection:An Analysis of 242 Cases
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作者 Feibao CHEN Changming ZHANG +6 位作者 Chen CHEN Ping JI Chanjuan ZHANG Yanbo LI Hao WANG Baobao GU Yanting YANG 《Medicinal Plant》 2024年第2期61-64,共4页
[Objectives]To investigate the clinical effect of Yinhuang Qingfei capsules in the treatment of asymptomatic and mild/common severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.[Methods]A total of 362... [Objectives]To investigate the clinical effect of Yinhuang Qingfei capsules in the treatment of asymptomatic and mild/common severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.[Methods]A total of 362 patients with SARS-CoV-2 infection were divided into the treatment group with 242 patients and control group with 120 patients according to their treatment regimen.The patients in the control group were given standard treatment regimen and those in the treatment group were given Yinhuang Qingfei capsules in addition to the treatment in the control group.The two groups were observed in terms of average length of hospital stay,mean time for nucleic acid clearance,TCM syndrome score,and progression to severe/critical illness,and clinical outcome was compared between the two groups.[Results]There was a significant difference in the overall response rate between the treatment group and the control group[97.52%(236/242)vs 95.00%(114/120),P<0.05].Compared with the control group,the treatment group had significantly shorter length of hospital stay and time for nucleic acid clearance(P<0.05).After 7 days of treatment,both groups had a significant change in TCM syndrome score,and there was a significant difference in TCM syndrome score between the two groups(P<0.05);after 15 days of treatment,both groups had a TCM syndrome score of 0.Progression to severe/critical illness was not observed in either group.[Conclusions]Compared with the standard treatment regimen alone,standard treatment regimen combined with Yinhuang Qingfei capsules can effectively shorten the length of hospital stay and time for nucleic acid clearance and improve TCM symptoms in patients with asymptomatic and mild/common SARS-CoV-2 infection. 展开更多
关键词 Severe acute respiratory syndrome coronavirus 2 infection ASYMPTOMATIC Mild/common Yinhuang Qingfei capsules
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CAP1400主管道研制中大型不锈钢锻件制造技术的提升
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作者 郑建能 陈红宇 +3 位作者 刘鑫刚 周夏 李连龙 王凯 《大型铸锻件》 2025年第4期7-10,21,共5页
国内企业依托CAP1400主管道制造技术研制,包括120 t级超大型超低碳控氮奥氏体不锈钢电渣重熔技术、大型异形奥氏体不锈钢坯料锻造技术、大型厚壁承压管道弯曲成形技术、大型异形奥氏体不锈钢管道热处理变形控制及性能控制技术,形成系统... 国内企业依托CAP1400主管道制造技术研制,包括120 t级超大型超低碳控氮奥氏体不锈钢电渣重熔技术、大型异形奥氏体不锈钢坯料锻造技术、大型厚壁承压管道弯曲成形技术、大型异形奥氏体不锈钢管道热处理变形控制及性能控制技术,形成系统、稳定的大型核电锻造主管道制造技术。研究成果推广应用于多种高端不锈钢锻件制造,使我国大型不锈钢锻件制造技术得到显著提升。 展开更多
关键词 cap1400 主管道 不锈钢锻件 制造技术
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公路预防性养护中CAP抗滑封层技术的应用效果分析 被引量:1
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作者 何星星 《交通世界》 2025年第4期114-116,共3页
为有效改善旧沥青路面路用性能,提高沥青废料循环使用率,降低公路建设成本,以某高速公路项目为背景,选取局部病害路段作为试验段,对沥青还原剂(CAP)抗滑封层技术的具体应用进行综合探究,介绍了原材料及施工工艺要求,并对沥青路面CAP再... 为有效改善旧沥青路面路用性能,提高沥青废料循环使用率,降低公路建设成本,以某高速公路项目为背景,选取局部病害路段作为试验段,对沥青还原剂(CAP)抗滑封层技术的具体应用进行综合探究,介绍了原材料及施工工艺要求,并对沥青路面CAP再生沥青封层处理前后的抗滑及抗车辙性能进行分阶段检测评定。检测结果显示:①采取CAP抗滑封层处理后,沥青路面车辙指数(RDI)及抗滑指数(SRI)均有明显增大,路面抗变形及抗滑能力得到提升;②相较于CAP再生沥青封层交工阶段,道路运营2年后,沥青路面车辙指数(RDI)及抗滑指数(SRI)下降幅度较小,充分表明CAP抗滑封层处理后的沥青路面具有良好的抗滑、抗变形及耐久性能。 展开更多
关键词 预防性养护 cap 抗滑性能 抗车辙性能
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Common bile duct stump stones and recurrent acute pancreatitis after Roux-en-Y choledochojejunostomy in a child:A case report 被引量:1
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作者 Jian-Feng Li Min-Jian Xie +6 位作者 Jin-Xiu Wei Cheng-Ning Yang Guang-Wen Chen Li-Qun Li Yi-Na Zhao Li-Jian Liu Sheng Xie 《World Journal of Gastrointestinal Surgery》 2025年第2期291-297,共7页
BACKGROUND Pancreaticobiliary maljunction(PBM)is a rare congenital abnormality in pancreaticobiliary duct development.PBM is commonly found in children,and it often leads to acute pancreatitis and other diseases as a ... BACKGROUND Pancreaticobiliary maljunction(PBM)is a rare congenital abnormality in pancreaticobiliary duct development.PBM is commonly found in children,and it often leads to acute pancreatitis and other diseases as a result of pancreaticobiliary reflux.Roux-en-Y choledochojejunostomy is a common surgical method for the treatment of PBM,but there are several associated complications that may occur after this operation.CASE SUMMARY The patient,a 12-year-old female,was hospitalized nearly 20 times in 2021 for recurrent acute pancreatitis.In 2022,she was diagnosed with PBM and underwent laparoscopic common bile duct resection and Roux-en-Y choledochojejunostomy in a tertiary hospital.In the first year after surgery,the patient had more than 10 recurrent acute pancreatitis episodes.After undergoing abdominal computed tomography and other examinations,she was diagnosed with“residual bile duct stones and recurrent acute pancreatitis”.On January 30,2024,the patient was admitted to our hospital due to recurrent upper abdominal pain and was cured through endoscopic retrograde cholangiopancreatography.CONCLUSION This article reports a case of a child with distal residual common bile duct stones and recurrent acute pancreatitis after Roux-en-Y choledochojejunostomy for PBM.The patient was cured through endoscopic retrograde cholangiopancreatography. 展开更多
关键词 Pancreaticobiliary maljunction Roux-en-Y choledochojejunostomy common bile duct stones Recurrent acute pancreatitis Endoscopic retrograde cholangiopancreatography Case report
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压电-光催化作用对CaP在TC4表面ZnO纳米棒阵列上的沉积机制
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作者 陈天濠 周清 许晓键 《南京航空航天大学学报(自然科学版)》 北大核心 2025年第1期139-146,共8页
由于钛合金(TC4)的表面惰性,其作为骨植入材料在生物相容性方面存在局限性。本文采用水热生长法在TC4表面成功构建了具有压电和光催化特性的ZnO纳米棒阵列涂层。在模拟体液中进行的仿生矿化实验中,通过对样品表面施加振动和紫外光照射,... 由于钛合金(TC4)的表面惰性,其作为骨植入材料在生物相容性方面存在局限性。本文采用水热生长法在TC4表面成功构建了具有压电和光催化特性的ZnO纳米棒阵列涂层。在模拟体液中进行的仿生矿化实验中,通过对样品表面施加振动和紫外光照射,探究压电-光催化共同作用下对磷酸钙(CaP)的沉积的影响,提出了一种新的仿生矿化沉积CaP的方法。结果表明:氧化锌的压电-光催化共同作用显著增强了CaP的沉积量,优于单独压电或光催化作用下的沉积量。此外,单独的振动或紫外光照也表现出较未处理样品更高的CaP沉积量。本文在钛合金片表面制备了ZnO纳米棒阵列,并提出了一种利用压电-光共同作用增强CaP沉积的方法,为涂层技术及生物医用材料的进一步开发应用提供了一种新思路。 展开更多
关键词 TC4 氧化锌 压电-光催化 磷酸钙 仿生矿化
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Rhizobium-Based Biofertilizer for Rational Use of Mineral Nitrogen Fertilizers: Case Study of Common Bean Cultivation in Senegal
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作者 Adama Diouf 《American Journal of Plant Sciences》 2025年第1期133-145,共13页
Synthetic fertilizers are widely used to address the urgent challenge of ensuring food supplies for a growing world population in the context of climate change. However, their industrial production and use in agricult... Synthetic fertilizers are widely used to address the urgent challenge of ensuring food supplies for a growing world population in the context of climate change. However, their industrial production and use in agriculture have a negative impact on the environment and consequently on human health. While chemical fertilizers may not have to be abandoned in agricultural production systems, limiting their use could help to make agriculture sustainable and resilient to climate change. In Senegal, the level of mineral fertilizers used in market gardening has become alarming in the Niayes area. As a result, microbial biotechnologies have been promoted for biofertilizer production of common bean (Phaseolus vulgaris L.) cultivation. Rhizobial inoculums have thus been used to reduce the rate of chemical nitrogen fertilizers being applied in cropping systems. Several investigations in the laboratory, on experimental stations and in the field have shown a possibility of a significant reduction in the use of nitrogen fertilizers in common bean production. Conventional mineral fertilization use can be reduced from over 120 kg N/ha to 20 kg N/ha. This contributes both to a very significant reduction in the application rate with the same level of yield and to an improvement in the standard of living. In addition, the environmental impact of using chemical fertilizers can be mitigated. This study is a contribution to the promotion of biofertilizers adoption in agricultural systems. 展开更多
关键词 Sustainable Agriculture Mineral Fertilizer Inoculation RHIZOBIUM common Bean
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Coincidence Point and Common Fixed Point Theorems on b-Metric-like Spaces
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作者 LUO Ting 《应用数学》 北大核心 2025年第3期831-840,共10页
In this paper,we establish common fixed point theorems for expansive map?pings on b-metric-like space and coincidence point for f-weakly isotone increasing mappings in partially ordered b-metric-like space.The main re... In this paper,we establish common fixed point theorems for expansive map?pings on b-metric-like space and coincidence point for f-weakly isotone increasing mappings in partially ordered b-metric-like space.The main results generalize and extend several well-known comparable results from the existing literature.Moreover,some examples are provided to illustrate the main results. 展开更多
关键词 b-metric-like space Partially ordered b-metric-like space Expansive mapping f-weakly isotone mapping Coincidence point common fixed point
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基于广义Capped l_(2,q)范数的多项式正则化线性判别
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作者 杨源 周跃进 《哈尔滨商业大学学报(自然科学版)》 2025年第1期96-103,共8页
线性判别分析(LDA)经常存在类内散射矩阵不可逆且对异常值和噪声十分敏感等问题.为了解决这些问题,提出了一种基于广义Capped范数的多项式正则化线性判别分析(PGCLDA)方法.通过在目标函数中引入l_(2,q)范数并设置阈值,可以减少噪声的影... 线性判别分析(LDA)经常存在类内散射矩阵不可逆且对异常值和噪声十分敏感等问题.为了解决这些问题,提出了一种基于广义Capped范数的多项式正则化线性判别分析(PGCLDA)方法.通过在目标函数中引入l_(2,q)范数并设置阈值,可以减少噪声的影响、识别出数据的异常点、降低特大范数对投影方向的影响.把类间散射矩阵映射到多项式函数中增加矩阵的迹,使不同类别之间的样本距离更远,提高判别能力.对类内散射矩阵增加正则化项,避免不可逆的情形发生.在模拟和真实数据应用结果表明,本方法比其他类似的方法在减少异常值和噪声的影响与提高类别判别能力等方面更具有优越性且有更高的预测准确性. 展开更多
关键词 线性判别分析 capped l_(2 q)范数 多项式函数 散射矩阵 噪声处理 正则化
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Risk factors for bile leakage after laparoscopic common bile duct exploration in older patients with choledocholithiasis
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作者 Ruo-Fei Xiong Shan-Shan Lu +2 位作者 Zhi-Ming Wu Hong-Jun Huang Tao Xiao 《World Journal of Gastrointestinal Surgery》 2025年第4期150-160,共11页
BACKGROUND At present,there are few studies on the risk factors for bile leakage after laparoscopic common bile duct exploration(LCBDE)for older patients with choledocholithiasis.AIM To identify the potential risk fac... BACKGROUND At present,there are few studies on the risk factors for bile leakage after laparoscopic common bile duct exploration(LCBDE)for older patients with choledocholithiasis.AIM To identify the potential risk factors for bile leakage after LCBDE in older patients.METHODS A retrospective,single-center observational analysis was performed on patients aged≥70 years with choledocholithiasis treated by LCBDE who were admitted to our center between January 2011 and August 2022.The included patients were divided into non-bile leakage and bile leakage groups.Risk factors were determined by analyzing the observation indicators.RESULTS Seventy older patients with choledocholithiasis who underwent LCBDE were included.Univariate analysis showed that positive culture of bile bacteria was a risk factor for bile leakage after LCBDE(P<0.05).We further analyzed the bile bacteria,and univariate analysis showed that Enterococcus faecalis(E.faecalis)(P<0.05)and Pseudomonas aeruginosa(P<0.05)were associated with an increased risk of postoperative bile leakage in older patients(P<0.05).Multivariate analysis showed that E. faecalis was an independent risk factor for postoperative bile leakage in older patients (P < 0.05). Theresults of antibiotic sensitivity analysis showed that E. faecalis had 100% susceptibility to penicillin, ampicillin,linezolid, vancomycin, and furantoin.CONCLUSIONE. faecalis-associated biliary tract infection is an independent risk factor for bile leakage after LCBDE in olderpatients with choledocholithiasis. We suggest coverage with antibiotics to which E. faecalis is sensitive. 展开更多
关键词 Bile leakage common bile duct stones Older patients Laparoscopic common bile duct exploration Enterococcus faecalis Antibiotic sensitivity
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Antibacterial Mechanism of Common Cnidium Fruit Based on Network Pharmacology
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作者 Yingbing HE Zengli WANG Quan SHI 《Medicinal Plant》 2025年第5期1-5,10,共6页
[Objectives]To investigate the antibacterial mechanism of Common Cnidium Fruit using network pharmacology.[Methods]Active components and targets of Common Cnidium Fruit were screened and obtained using the TCMSP datab... [Objectives]To investigate the antibacterial mechanism of Common Cnidium Fruit using network pharmacology.[Methods]Active components and targets of Common Cnidium Fruit were screened and obtained using the TCMSP database and HIT2.0 database.The collected targets were intersected with antibacterial/bacteriostatic targets obtained from the GeneCards database and OMIM database to identify the antibacterial/bacteriostatic targets of Common Cnidium Fruit.The active component-target network diagram of Common Cnidium Fruit was constructed using Cytoscape software and topological analysis was performed.GO enrichment analysis was performed on the target genes using the DAVID database.[Results]Screening yielded 25 active components of Common Cnidium Fruit,corresponding to 77 targets.Analysis identified 25 core antibacterial/bacteriostatic targets for Common Cnidium Fruit.Network analysis indicated that Common Cnidium Fruit may exert antibacterial/bacteriostatic effects through active components such asβ-sitosterol,stigmasterol,and xanthoxylin,while activating the body's immune regulatory functions by acting on targets including CASP3,PTGS2,BCL2,JUN,and ESR1.[Conclusions]Common Cnidium Fruit may exert antibacterial/bacteriostatic effects through multiple pathways via a mechanism involving multiple components,multiple targets,and multiple pathways. 展开更多
关键词 common Cnidium FRUIT BACTERIOSTATIC ACTIVITY MECHANISM of action Network PHARMACOLOGY
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Gap-free genome assembly and metabolomics analysis of common bean provide insights into genomic characteristics and metabolic determinants of seed coat pigmentation
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作者 Bo Zhao Hui Zhang +13 位作者 Qin Zhao Rina Wu Qian You Bihui Wang Yiming Wang Zhilan Yan Peng Wang Chunguo Huang Wei Zhang Youmei Wang Xiaopeng Hao Jing Wu Lixiang Wang Zhaosheng Kong 《Journal of Genetics and Genomics》 2025年第6期852-855,共4页
Common bean(Phaseolus vulgaris L.)is a vital source of protein and essential nutrients for human consumption and plays a key role in sustainable agriculture due to its nitrogen-fixing ability(Nadeem et al.,2021).Kidne... Common bean(Phaseolus vulgaris L.)is a vital source of protein and essential nutrients for human consumption and plays a key role in sustainable agriculture due to its nitrogen-fixing ability(Nadeem et al.,2021).Kidney beans,a subcategory of dry common beans,are highly valued for their rich protein,dietary fiber,low fat content,and various trace elements(Garcia-Cordero et al.,2021).Despite the release of several de novo genome assemblies(Goodstein et al.,2012;Schmutz et al.,2014;Vlasova et al.,2016;Cortinovis et al.,2024),existing common bean genomes remain incomplete,particularly in complex regions such as centromeres and telomeres,limiting a comprehensive understanding of the genomic landscape. 展开更多
关键词 trace elements garcia cordero genomic characteristics gap free genome assembly metabolomics analysis common beansare de novo genome assemblies goodstein sustainable agriculture common bean
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The Role of the Digital Economy in Promoting Common Prosperity:Mechanisms,Challenges,and Strategies
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作者 Yang Ying Yuan Zheng 《Contemporary Social Sciences》 2025年第4期1-15,共15页
Based on Marxist theory of social reproduction and the dual dimensions of“wealth”and“common prosperity,”this study examines the impact of the digital economy in promoting common prosperity across the four key stag... Based on Marxist theory of social reproduction and the dual dimensions of“wealth”and“common prosperity,”this study examines the impact of the digital economy in promoting common prosperity across the four key stages:production,distribution,exchange,and consumption.The findings indicate that the digital economy fosters productivity growth and plays significant regulatory roles in primary distribution,redistribution,and third distribution.Moreover,the digital economy overcomes the spatiotemporal constraints of exchange and enhances consumption equality,thereby generating both“wealth”and“common prosperity”effects.However,there are still persistent challenges,including the displacement of traditional outputs,monopolization by digital platforms,the impact on employment,and income disparities arising from the digital divide.To address these challenges,we recommend optimizing the development policies of the digital economy,accelerating the growth of digital productivity,strengthening regulation of the digital platforms,building new talent supply systems,actively addressing the digital divide,and advancing market-oriented reform of data elements to better promote common prosperity. 展开更多
关键词 digital economy common prosperity WEALTH shared prosperity
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上皮性卵巢癌CapG表达与Treg细胞浸润及患者预后的相关性研究
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作者 陆灿灿 张晶晶 +2 位作者 金银瑛 杨欣 乔涵 《现代妇产科进展》 2025年第6期437-440,共4页
目的:探究上皮性卵巢癌(EOC)中巨噬细胞加帽蛋白(CapG)表达与调节性T细胞(Treg)浸润及患者预后的相关性。方法:免疫组化法检测EOC、卵巢良性肿瘤及正常卵巢组织中CapG表达及Treg细胞浸润,探讨CapG表达和Treg细胞浸润与EOC临床病理特征... 目的:探究上皮性卵巢癌(EOC)中巨噬细胞加帽蛋白(CapG)表达与调节性T细胞(Treg)浸润及患者预后的相关性。方法:免疫组化法检测EOC、卵巢良性肿瘤及正常卵巢组织中CapG表达及Treg细胞浸润,探讨CapG表达和Treg细胞浸润与EOC临床病理特征及预后的关系,分析CapG表达和Treg细胞浸润之间的相关性。结果:CapG在EOC组织中的表达明显高于卵巢良性肿瘤组织及正常卵巢组织,且CapG表达与肿瘤分期、分级和有无淋巴结转移有关。EOC组织中Treg细胞浸润较卵巢良性肿瘤组织及正常卵巢组织均明显上调,与EOC分期、分级、有无淋巴结转移和病理类型有关。EOC中CapG表达与Treg细胞浸润呈显著正相关。CapG表达及Treg细胞浸润与EOC不良预后有关。结论:CapG与Treg细胞可能参与了EOC发生、发展,有望成为预测EOC预后的指标。 展开更多
关键词 上皮性卵巢癌 巨噬细胞加帽蛋白 调节性T细胞 预后
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High-acclimation capacity for growth and role of soil fertility after long-range transfer of Betula pendula and B.pubescens between Finland and Italy
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作者 Oksanen Elina Silfver Tarja +6 位作者 Ghelardini Luisa Pecori Francesco Nieminen Kaisa Ruhanen Helena Rousi Matti Santini Alberto Mikola Juha 《Journal of Forestry Research》 2025年第3期152-170,共19页
The acclimation capacity of Betula pendula and Betula pubescens was studied over 4 years in common gardens in central Italy(43°N)and southern(61°N)and northern Finland(67°N),representing drastically dif... The acclimation capacity of Betula pendula and Betula pubescens was studied over 4 years in common gardens in central Italy(43°N)and southern(61°N)and northern Finland(67°N),representing drastically different photoperiod and climate in temperate,boreal and subarctic vegetation zones.Two study sites that differed in soil fertility were established at each location,giving a total of six common gardens.The birch material was micropropagated from naturally regenerated stands of B.pendula and B.pubescens from Susa Valley and Rochemolle Valley in northern Italy,Punkaharju in southern Finland and Kittilä in northern Finland.The plants were measured for height growth,stem diameter,leaf chlorophyll content,leaf herbivory and pathogen damage.The effects of soil fertility on the common garden results were also analyzed.The results showed high acclimation capacity of B.pendula and B.pubescens after a long-range transfer from southern to northern Europe,despite the major shift in climate and photoperiod.First-year growth on average was best in boreal southern Finland for all origins.Betula pendula grew more than B.pubescens in Italy and southern Finland,while B.pubescens grew more in northern Finland and better tolerated the northward transfer.The height growth of origins showed a clear latitude gradient from slowly growing northern to fast growing southern origins in the nursery and laboratory,but not in the field.Soil fertility explained a significant part of variation among locations not only for growth variables,but also for leaf chlorophyll content and leaf herbivory and pathogen damage.Leaf herbivore and pathogen damage was greatest in southern Finland.Our results demonstrate good survival of birch from northern Italy in Finnish conditions and support the possibility of long-range south-to-north transfer of Betula species to provide resistant planting material in boreal forests for the rapidly changing climate. 展开更多
关键词 BIRCH Betula sp. common garden Climate warming HERBIVORY
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The Role of Rural Revitalization Investment Funds in Advancing Common Prosperity: Mechanisms, Challenges, and Strategies
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作者 Ruihuan Wang 《Proceedings of Business and Economic Studies》 2025年第1期50-54,共5页
The Rural Revitalization Investment Fund serves as a crucial financial instrument for advancing rural revitalization and promoting common prosperity.It aims to address financing and land-use challenges faced by rural ... The Rural Revitalization Investment Fund serves as a crucial financial instrument for advancing rural revitalization and promoting common prosperity.It aims to address financing and land-use challenges faced by rural industries through financial support while fostering coordinated industrial development.The fund primarily operates through equity direct investment and the“mother fund+direct investment”model,with an increasing emphasis on combining government guidance with social capital participation.However,challenges persist,including a shortage of professional talent,insufficient institutional innovation,and weak industrial foundations in rural areas.To enhance the fund’s effectiveness,it is recommended to strengthen professional team management,clearly define its strategic positioning,implement strict supervision,innovate operational mechanisms,and prioritize environmental and social responsibility.These measures will ensure that the fund contributes to rural revitalization while promoting sustainable development and common prosperity. 展开更多
关键词 Rural revitalization investment fund Financial support common prosperity
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