A deeper understanding of the biological events occurring when bioprocess parameters changed will be of great value in improving the monoclonal antibodies (mAbs) production. Design of experiment (DoE) was applied to i...A deeper understanding of the biological events occurring when bioprocess parameters changed will be of great value in improving the monoclonal antibodies (mAbs) production. Design of experiment (DoE) was applied to investigate the effect of process parameters (pH, temperature shift and dissolve oxygen (DO)) on protein titer. The key metabolites connecting the critical process parameters (CPPs) with monoclonal antibody production were identified by different chemometrics tools. Finally, the biological events of marker metabolites relating with titer improvement were concluded. pH and temperature shift were identified as CPPs that affect the target protein titer. A series of metabolites influenced by the altered CPPs and correlated with protein titer were screened by principal component analysis (PCA) and Pearson' correlation test. The marker metabolites and their pathways linking CPPs to target protein titer in different culture phases were summarized. Metabolomics and chemometrics are promising data-driven tools to shine light into the biological black box between the bioprocess parameters and process performance.展开更多
Using approximation technique, we introduce the concepts of canonical extension and symmetrio integral for jump process and obtain some results in the chaotic form.
全程自养脱氮(CANON)工艺在110 d时,总氮去除负荷(NRR)达到了1.8 kg N/(m3·d)。在高溶解氧(DO)的条件下,CANON工艺脱稳失活,脱氮性能急剧下降,ANAMMOX菌受到抑制。采取减少曝气量,降低反应器中的DO,可以有效恢复CANON工艺的脱氮性...全程自养脱氮(CANON)工艺在110 d时,总氮去除负荷(NRR)达到了1.8 kg N/(m3·d)。在高溶解氧(DO)的条件下,CANON工艺脱稳失活,脱氮性能急剧下降,ANAMMOX菌受到抑制。采取减少曝气量,降低反应器中的DO,可以有效恢复CANON工艺的脱氮性能。在第327天时,进水氨氮浓度高达1 245.5 mg/L,氨氮和总氮去除效率分别达到98.4%和80.3%,而NRR高达了1 kg N/(m3·d)。此后,CANON工艺脱氮效果较为稳定,表明CANON工艺恢复效果明显。SEM分析表明,颗粒污泥表面附着大量的短杆状细菌,细菌之间联系紧密,部分颗粒污泥表面呈现网状结构,细菌之间通过丝状物联系形成颗粒污泥。能谱分析表明,颗粒污泥表面含有O、Na、P、Fe、Ca、C和Si等元素。展开更多
基金Supported by the Science and Technology Development Program of Zhejiang Province(2017C03003)
文摘A deeper understanding of the biological events occurring when bioprocess parameters changed will be of great value in improving the monoclonal antibodies (mAbs) production. Design of experiment (DoE) was applied to investigate the effect of process parameters (pH, temperature shift and dissolve oxygen (DO)) on protein titer. The key metabolites connecting the critical process parameters (CPPs) with monoclonal antibody production were identified by different chemometrics tools. Finally, the biological events of marker metabolites relating with titer improvement were concluded. pH and temperature shift were identified as CPPs that affect the target protein titer. A series of metabolites influenced by the altered CPPs and correlated with protein titer were screened by principal component analysis (PCA) and Pearson' correlation test. The marker metabolites and their pathways linking CPPs to target protein titer in different culture phases were summarized. Metabolomics and chemometrics are promising data-driven tools to shine light into the biological black box between the bioprocess parameters and process performance.
基金Supported in part by National Natural Science Foundation of China.
文摘Using approximation technique, we introduce the concepts of canonical extension and symmetrio integral for jump process and obtain some results in the chaotic form.
文摘全程自养脱氮(CANON)工艺在110 d时,总氮去除负荷(NRR)达到了1.8 kg N/(m3·d)。在高溶解氧(DO)的条件下,CANON工艺脱稳失活,脱氮性能急剧下降,ANAMMOX菌受到抑制。采取减少曝气量,降低反应器中的DO,可以有效恢复CANON工艺的脱氮性能。在第327天时,进水氨氮浓度高达1 245.5 mg/L,氨氮和总氮去除效率分别达到98.4%和80.3%,而NRR高达了1 kg N/(m3·d)。此后,CANON工艺脱氮效果较为稳定,表明CANON工艺恢复效果明显。SEM分析表明,颗粒污泥表面附着大量的短杆状细菌,细菌之间联系紧密,部分颗粒污泥表面呈现网状结构,细菌之间通过丝状物联系形成颗粒污泥。能谱分析表明,颗粒污泥表面含有O、Na、P、Fe、Ca、C和Si等元素。
