This study explores CAMTA genes in the rare and endangered Chinese plant species,Liriodendron chinense.Despite the completion of whole-genome sequencing,the roles of CAMTA genes in calcium regulation and stress respon...This study explores CAMTA genes in the rare and endangered Chinese plant species,Liriodendron chinense.Despite the completion of whole-genome sequencing,the roles of CAMTA genes in calcium regulation and stress responses in this species remain largely unexplored.Within the L.chinense genome,we identified two CAMTA genes,Lchi09764 and Lchi222536,characterized by four functional domains:CG-1,TIG,ANK repeats,and IQ motifs.Our analyses,including phylogenetic investigations,cis-regulatory element analyses,and chromosomal location studies,aim to elucidate the defining features of CAMTA genes in L.chinense.Applying Weighted Gene Co-Expression Network Analysis(WGCNA),we explored the impact of CAMTA genes on different organs and their regulation under abiotic stress conditions.The identification of significant gene modules and the prediction of promoter binding sites revealed co-expressed genes associated with CAMTA transcription factors.In summary,this study provides initial insights into CAMTA genes in L.chinense,laying the groundwork for future research on their evolution and biological roles.This knowledge enhancement contributes to a better understanding of plant responses to environmental stress—an essential aspect of plant biology.展开更多
采用生物信息学方法鉴定了巨菌草(Cenchrus fungigraminus Z. X. Lin&D.M. Lin&S. R. Lan sp. nov.)中CAMTA(calmodulin-binding transcription activator)转录因子的家族成员,并对其蛋白序列的理化性质、基因结构、保守基序、...采用生物信息学方法鉴定了巨菌草(Cenchrus fungigraminus Z. X. Lin&D.M. Lin&S. R. Lan sp. nov.)中CAMTA(calmodulin-binding transcription activator)转录因子的家族成员,并对其蛋白序列的理化性质、基因结构、保守基序、保守功能结构域、系统进化发育关系、启动子区域顺式作用元件进行了分析;探讨了巨菌草CAMTA((CfuCAMTA))家族成员在干旱胁迫下转录水平的变化,以及CfuCAMTA转录因子对响应干旱胁迫的影响.结果表明:巨菌草CAMTA家族共有15个成员,其中仅CfuCAMTA10和CfuCAMTA11较为稳定,不稳定系数小于40;多数成员为不稳定蛋白质;巨菌草和紫象草CAMTA基因的亲缘关系最近;4个CAMTA转录因子的表达水平随干旱时间的延长,在第14天表达量达到最大,说明这些CAMTA参与了干旱胁迫的响应.展开更多
The Arabidopsis thaliana Calmodulin-binding Transcription Activator(CAMTA)transcription factors CAMTA1,CAMTA2,and CAMTA3(CAMTA123)serve as master regulators of salicylic acid(SA)-mediated immunity,repressing the biosy...The Arabidopsis thaliana Calmodulin-binding Transcription Activator(CAMTA)transcription factors CAMTA1,CAMTA2,and CAMTA3(CAMTA123)serve as master regulators of salicylic acid(SA)-mediated immunity,repressing the biosynthesis of SA in healthy plants.Here,we show that CAMTA123 also repress the biosynthesis of pipecolic acid(Pip)in healthy plants.Loss of CAMTA123 function resulted in the induction of AGD2-like defense response protein 1(ALD1),which encodes an enzyme involved in Pip biosynthesis.Induction of ALD1 resulted in the accumulation of high levels of Pip,which brought about increased levels of the SA receptor protein NPR1 without induction of NPR1 expression or requirement for an increase in SA levels.Pip-mediated induction of ALD1 and genes regulating the biosynthesis of SA—CBP60g,SARD1,PAD4,and EDS1—was largely dependent on NPR1.Furthermore,Pip-mediated increase in NPR1 protein levels was associated with priming of Pip and SA biosynthesis genes to induction by low levels of SA.Taken together,our findings expand the role for CAMTA123 in regulating key immunity genes and suggest a working model whereby loss of CAMTA123 repression leads to the induction of plant defense genes and initiation of SAR.展开更多
Two signal molecules, salicylic acid (SA) and N-hydroxypipecolic acid (NHP), play critical roles in plant immunity. The biosynthetic genes of both compounds are positively regulated by master immune-regulating transcr...Two signal molecules, salicylic acid (SA) and N-hydroxypipecolic acid (NHP), play critical roles in plant immunity. The biosynthetic genes of both compounds are positively regulated by master immune-regulating transcription factors SARD1 and CBP60g. However, the relationship between the SA and NHP pathways is unclear. CALMODULIN-BINDING TRANSCRIPTION FACTOR 1 (CAMTA1), CAMTA2, and CAMTA3 are known redundant negative regulators of plant immunity, but the underlying mechanism also remains largely unknown. In this study, through chromatin immunoprecipitation and electrophoretic mobility shift assays, we uncovered that CBP60g is a direct target of CAMTA3, which also negatively regulates the expression of SARD1, presumably via an indirect effect. The autoimmunity of camta3-1 is suppressed by sard1 cbp60g double mutant as well as ald1 and fmo1, two single mutants defective in NHP biosynthesis. Interestingly, a suppressor screen conducted in the camta1/ 2/ 3 triple mutant background yielded various mutants blocking biosynthesis or signaling of either SA or NHP, leading to nearly complete suppression of the extreme autoimmunity of camta1/ 2/ 3, suggesting that the SA and NHP pathways can mutually amplify each other. Together, these results reveal that CAMTAs repress the biosynthesis of SA and NHP by modulating the expression of SARD1 and CBP60g, and that the SA and NHP pathways are coordinated to optimize plant immune response.展开更多
基金This research was funded by the National Natural Science Foundation of China(No.31971682)the Research Startup Fund for High-Level and High-Educated Talents of Nanjing Forestry University.
文摘This study explores CAMTA genes in the rare and endangered Chinese plant species,Liriodendron chinense.Despite the completion of whole-genome sequencing,the roles of CAMTA genes in calcium regulation and stress responses in this species remain largely unexplored.Within the L.chinense genome,we identified two CAMTA genes,Lchi09764 and Lchi222536,characterized by four functional domains:CG-1,TIG,ANK repeats,and IQ motifs.Our analyses,including phylogenetic investigations,cis-regulatory element analyses,and chromosomal location studies,aim to elucidate the defining features of CAMTA genes in L.chinense.Applying Weighted Gene Co-Expression Network Analysis(WGCNA),we explored the impact of CAMTA genes on different organs and their regulation under abiotic stress conditions.The identification of significant gene modules and the prediction of promoter binding sites revealed co-expressed genes associated with CAMTA transcription factors.In summary,this study provides initial insights into CAMTA genes in L.chinense,laying the groundwork for future research on their evolution and biological roles.This knowledge enhancement contributes to a better understanding of plant responses to environmental stress—an essential aspect of plant biology.
文摘采用生物信息学方法鉴定了巨菌草(Cenchrus fungigraminus Z. X. Lin&D.M. Lin&S. R. Lan sp. nov.)中CAMTA(calmodulin-binding transcription activator)转录因子的家族成员,并对其蛋白序列的理化性质、基因结构、保守基序、保守功能结构域、系统进化发育关系、启动子区域顺式作用元件进行了分析;探讨了巨菌草CAMTA((CfuCAMTA))家族成员在干旱胁迫下转录水平的变化,以及CfuCAMTA转录因子对响应干旱胁迫的影响.结果表明:巨菌草CAMTA家族共有15个成员,其中仅CfuCAMTA10和CfuCAMTA11较为稳定,不稳定系数小于40;多数成员为不稳定蛋白质;巨菌草和紫象草CAMTA基因的亲缘关系最近;4个CAMTA转录因子的表达水平随干旱时间的延长,在第14天表达量达到最大,说明这些CAMTA参与了干旱胁迫的响应.
基金This work was supported by grants to M.F.T.from Michigan AgBioRe-search(M1CL02415)the MSU Foundation and infrastructure support from the Chemical Sciences,Geosciences,and Biosciences Division,Of fice of Basic Energy Sciences,US Department of Energy(DE-FG02-91ER20021).
文摘The Arabidopsis thaliana Calmodulin-binding Transcription Activator(CAMTA)transcription factors CAMTA1,CAMTA2,and CAMTA3(CAMTA123)serve as master regulators of salicylic acid(SA)-mediated immunity,repressing the biosynthesis of SA in healthy plants.Here,we show that CAMTA123 also repress the biosynthesis of pipecolic acid(Pip)in healthy plants.Loss of CAMTA123 function resulted in the induction of AGD2-like defense response protein 1(ALD1),which encodes an enzyme involved in Pip biosynthesis.Induction of ALD1 resulted in the accumulation of high levels of Pip,which brought about increased levels of the SA receptor protein NPR1 without induction of NPR1 expression or requirement for an increase in SA levels.Pip-mediated induction of ALD1 and genes regulating the biosynthesis of SA—CBP60g,SARD1,PAD4,and EDS1—was largely dependent on NPR1.Furthermore,Pip-mediated increase in NPR1 protein levels was associated with priming of Pip and SA biosynthesis genes to induction by low levels of SA.Taken together,our findings expand the role for CAMTA123 in regulating key immunity genes and suggest a working model whereby loss of CAMTA123 repression leads to the induction of plant defense genes and initiation of SAR.
文摘Two signal molecules, salicylic acid (SA) and N-hydroxypipecolic acid (NHP), play critical roles in plant immunity. The biosynthetic genes of both compounds are positively regulated by master immune-regulating transcription factors SARD1 and CBP60g. However, the relationship between the SA and NHP pathways is unclear. CALMODULIN-BINDING TRANSCRIPTION FACTOR 1 (CAMTA1), CAMTA2, and CAMTA3 are known redundant negative regulators of plant immunity, but the underlying mechanism also remains largely unknown. In this study, through chromatin immunoprecipitation and electrophoretic mobility shift assays, we uncovered that CBP60g is a direct target of CAMTA3, which also negatively regulates the expression of SARD1, presumably via an indirect effect. The autoimmunity of camta3-1 is suppressed by sard1 cbp60g double mutant as well as ald1 and fmo1, two single mutants defective in NHP biosynthesis. Interestingly, a suppressor screen conducted in the camta1/ 2/ 3 triple mutant background yielded various mutants blocking biosynthesis or signaling of either SA or NHP, leading to nearly complete suppression of the extreme autoimmunity of camta1/ 2/ 3, suggesting that the SA and NHP pathways can mutually amplify each other. Together, these results reveal that CAMTAs repress the biosynthesis of SA and NHP by modulating the expression of SARD1 and CBP60g, and that the SA and NHP pathways are coordinated to optimize plant immune response.
