不同于国内市场上的其它竞品,Calvin Klein Men's underwear凭借其主次分明的产品结构、亲民的价格设置、打破常规的产品设计、讲究的店面布局,大胆的位置选择以及线上线下同价的销售策略戎功赢得中国中高端男士,对于国内高端男士...不同于国内市场上的其它竞品,Calvin Klein Men's underwear凭借其主次分明的产品结构、亲民的价格设置、打破常规的产品设计、讲究的店面布局,大胆的位置选择以及线上线下同价的销售策略戎功赢得中国中高端男士,对于国内高端男士内衣品牌的发展有很高的借鉴价值。展开更多
Preached on July 8,1741,“Sinners in the Hands of an Angry God”remains Jonathan Edwards’s most famous written work and a classic of the Great Awakening of New England.The long-famed power of this sermon rests not on...Preached on July 8,1741,“Sinners in the Hands of an Angry God”remains Jonathan Edwards’s most famous written work and a classic of the Great Awakening of New England.The long-famed power of this sermon rests not only in his searing images of hellfire and the insecurity they elicit,but also in the Calvinistic thoughts Edwards imparts to his Enfield listeners.This paper mainly examines some basic doctrines of Calvinism Edward expresses in this sermon,such as God’s absolute sovereignty,Keywords:original sin,human depravity,and divine election,etc.展开更多
Carbon fixation by soil autotrophic microbes is an overlooked process in organic carbon anabolism,which is potentially affected by biochar.In this study,we quantified the abundance of functional genes cbbL and cbbM,ke...Carbon fixation by soil autotrophic microbes is an overlooked process in organic carbon anabolism,which is potentially affected by biochar.In this study,we quantified the abundance of functional genes cbbL and cbbM,key components of the widely distributed Calvin cycle,and combined this with Ribulose-1,5-bisphosphate carboxylase/oxygenase(RubisCO)enzyme activity assays and high-throughput sequencing of cbbL-and cbbM-harboring microbial communities to investigate the carbon fixation potential,activity,and community structure under biochar application in paddy and upland soils.Results showed that cbbL consistently dominated over cbbM in both paddy and upland soils,with higher abundances in paddy soils,driven by biochar amendment,rice growth stage,and rhizosphere effects.The rhizosphere acted as a hotspot for cbbL and cbbM genes and RubisCO activity in paddy soil.In upland soils,nitrogen availability(NH_(₄)^(+),dissolved organic nitrogen-DON),microbial biomass carbon,and labile carbon and nitrogen pools(dissolved organic carbon,N-acetyl-β-D-glucosaminidase)were consistently associated with cbbL abundance,underscoring their ecological role in soil CO_(2)fixation.In paddy soils,inorganic nitrogen(NH_(₄)^(+),NO_(3)^(⁻),NO_(2)^(⁻)),redox potential(Eh),and urease activity were the main predictors of cbbL abundance and the cbbL/16S ratio,while pH and nitrogen availability(NO_(2)^(⁻),DON)was mostly associated with cbbM/16S ratio.Biochar was the primary driver reshaping the structure of autotrophic microbial communities harboring cbbL and cbbM genes across different soil compartments,including surface soil,rhizosphere,and bulk soil.Pseudomonadota,Cyanobacteriota,Actinomycetota and Chloroflexota were dominant cbbL carriers,while Pseudomonadota,Actinomycetota and Myxococcota predominated in cbbM assemblages across soils.Biochar induced functional differentiation of facultative autotrophic taxa under different RubisCO forms by enhancing the abundance of Rhodopseudomonas in cbbM-bearing communities while decreasing it in cbbL-bearing ones.Furthermore,Calvin cycle-mediated CO_(2)fixation was found to couple with pathways including methylotrophy,methanotrophy,iron oxidation and respiration,nitrogen fixation and reduction,and arsenate reduction and detoxification.Collectively,the results of this study emphasize the importance of soil type,micro-environmental conditions,nitrogen status and the impact of biochar in shaping microbial carbon assimilation via the Calvin cycle pathway and the cbbL and cbbM-harboring microbial community.展开更多
Phosphoribulokinase （PRK）, a nuclear-encoded plastid-localized enzyme unique to the photosynthetic carbon reduction （Calvin） cycle, was cloned and characterized from the stramenopile alga Vaucheria litorea. This a...Phosphoribulokinase （PRK）, a nuclear-encoded plastid-localized enzyme unique to the photosynthetic carbon reduction （Calvin） cycle, was cloned and characterized from the stramenopile alga Vaucheria litorea. This alga is the source of plastids for the mollusc （sea slug） Elysia chlorotica which enable the animal to survive for months solely by photoautotrophic CO2 fixation. The 1633-bp V. litorea prk gene was cloned and the coding region, found to be interrupted by four introns, encodes a 405-amino acid protein. This protein contains the typical bipartite target sequence expected of nuclearencoded proteins that are directed to complex （i.e. four membrane-bound） algal plastids. De novo synthesis of PRK and enzyme activity were detected in E. chlorotica in spite of having been starved of V. litorea for several months. Unlike the algal enzyme, PRK in the sea slug did not exhibit redox regulation. Two copies of partial PRK-encoding genes were isolated from both sea slug and aposymbiotic sea slug egg DNA using PCR. Each copy contains the nucleotide region spanning exon 1 and part of exon 2 of V litorea prk, including the bipartite targeting peptide. However, the larger prk fragment also includes intron 1. The exon and intron sequences of prk in E. chlorotica and V/itorea are nearly identical. These data suggest that PRK is differentially regulated in V. litorea and E. chlorotica and at least a portion of the V. litorea nuclear PRK gene is present in sea slugs that have been starved for several months.展开更多
英语有句俗语＂My cup of tea＂,用更接地气的普通话该说成是＂我的菜＂。要时尚低调又要显出品位,你的这些挑剔口味Calvin Klein不仅全都满足,还力邀金宇彬来呈现这道新品大餐。新系列主题＂Life in the now＂讲述＂尽在此刻＂的故事,...英语有句俗语＂My cup of tea＂,用更接地气的普通话该说成是＂我的菜＂。要时尚低调又要显出品位,你的这些挑剔口味Calvin Klein不仅全都满足,还力邀金宇彬来呈现这道新品大餐。新系列主题＂Life in the now＂讲述＂尽在此刻＂的故事,而BAZAAR Jewelry也不忘给你送上新品腕表,看看他们到底是你的茶还是你的菜？展开更多
2006年11月,在时尚界独立风行了40年的卡尔文·克莱恩公司(Calvin Klein,Inc．)选址充斥着现代工业和神秘感的上海灵石路货仓,举行了一场名为“卡尔文·克莱恩的世界”(The World of Calvin Klein)的派对,这也是迄今为止CK在亚...2006年11月,在时尚界独立风行了40年的卡尔文·克莱恩公司(Calvin Klein,Inc．)选址充斥着现代工业和神秘感的上海灵石路货仓,举行了一场名为“卡尔文·克莱恩的世界”(The World of Calvin Klein)的派对,这也是迄今为止CK在亚洲举办的最大的时尚盛事。展开更多
Aerated drip irrigation(ADI)is an important practice for promoting soil fertility and crop productivity in greenhouse vegetable production,yet little research has comprehensively investigated its effects on the functi...Aerated drip irrigation(ADI)is an important practice for promoting soil fertility and crop productivity in greenhouse vegetable production,yet little research has comprehensively investigated its effects on the functional traits of carbon(C)-cycling microorganisms.In this study,we sought to assess the potential efficacy of ADI in increasing soil organic C(SOC)by changing soil microbial communities and the expressions of genes associated with C cycling.To this end,we adopted a metagenomic approach to compare the effects of ADI with three dissolved oxygen concentrations(10,15,and 20 mg L^(-1))during a three-season tomato cultivation experiment in northern China.The results revealed that the 10 mg L^(-1)treatment led to a significant increase in the abundance of korA/B genes(associated with the reductive tricarboxylic acid cycle)in the C fixation pathway,whereas the 15 mg L^(-1)treatment increased the abundances of cbbL/R and coxL/S genes associated with the Calvin cycle and carbon monoxide oxidation,respectively.In addition,based on a co-occurrence network analysis,we observed a positive correlation between cbbL and coxS.Interestingly,r-selected microorganisms,such as Proteobacteria and Actinobacteria,characterized by rapid cell multiplication and high biomass production,were identified as the primary contributors to C fixation and were the main predictors of SOC pools.In contrast,the 20 mg L^(-1)treatment was found to adversely influence C fixation,although the enhanced C degradation could be attributed to the extracellular enzymes secreted by K-selected microorganisms.Collectively,our findings indicate that ADI with dissolved oxygen concentrations 15 mg L^(-1)can promote SOC content by altering the life history strategies of r-selected microorganisms and genes associated with C fixation.These findings will provide valuable references for agroecosystem irrigation management,help improve soil fertility,and promote sustainable production.展开更多
基金Beijing Information Science and Technology University Scientific Research Project(1835010).
文摘Preached on July 8,1741,“Sinners in the Hands of an Angry God”remains Jonathan Edwards’s most famous written work and a classic of the Great Awakening of New England.The long-famed power of this sermon rests not only in his searing images of hellfire and the insecurity they elicit,but also in the Calvinistic thoughts Edwards imparts to his Enfield listeners.This paper mainly examines some basic doctrines of Calvinism Edward expresses in this sermon,such as God’s absolute sovereignty,Keywords:original sin,human depravity,and divine election,etc.
基金the National Natural Science Foundation of China(Grant No.42177107,42307043,21607125)Natural Science Foundation of Anhui Province,China(Grant No.2108085MC85).
文摘Carbon fixation by soil autotrophic microbes is an overlooked process in organic carbon anabolism,which is potentially affected by biochar.In this study,we quantified the abundance of functional genes cbbL and cbbM,key components of the widely distributed Calvin cycle,and combined this with Ribulose-1,5-bisphosphate carboxylase/oxygenase(RubisCO)enzyme activity assays and high-throughput sequencing of cbbL-and cbbM-harboring microbial communities to investigate the carbon fixation potential,activity,and community structure under biochar application in paddy and upland soils.Results showed that cbbL consistently dominated over cbbM in both paddy and upland soils,with higher abundances in paddy soils,driven by biochar amendment,rice growth stage,and rhizosphere effects.The rhizosphere acted as a hotspot for cbbL and cbbM genes and RubisCO activity in paddy soil.In upland soils,nitrogen availability(NH_(₄)^(+),dissolved organic nitrogen-DON),microbial biomass carbon,and labile carbon and nitrogen pools(dissolved organic carbon,N-acetyl-β-D-glucosaminidase)were consistently associated with cbbL abundance,underscoring their ecological role in soil CO_(2)fixation.In paddy soils,inorganic nitrogen(NH_(₄)^(+),NO_(3)^(⁻),NO_(2)^(⁻)),redox potential(Eh),and urease activity were the main predictors of cbbL abundance and the cbbL/16S ratio,while pH and nitrogen availability(NO_(2)^(⁻),DON)was mostly associated with cbbM/16S ratio.Biochar was the primary driver reshaping the structure of autotrophic microbial communities harboring cbbL and cbbM genes across different soil compartments,including surface soil,rhizosphere,and bulk soil.Pseudomonadota,Cyanobacteriota,Actinomycetota and Chloroflexota were dominant cbbL carriers,while Pseudomonadota,Actinomycetota and Myxococcota predominated in cbbM assemblages across soils.Biochar induced functional differentiation of facultative autotrophic taxa under different RubisCO forms by enhancing the abundance of Rhodopseudomonas in cbbM-bearing communities while decreasing it in cbbL-bearing ones.Furthermore,Calvin cycle-mediated CO_(2)fixation was found to couple with pathways including methylotrophy,methanotrophy,iron oxidation and respiration,nitrogen fixation and reduction,and arsenate reduction and detoxification.Collectively,the results of this study emphasize the importance of soil type,micro-environmental conditions,nitrogen status and the impact of biochar in shaping microbial carbon assimilation via the Calvin cycle pathway and the cbbL and cbbM-harboring microbial community.
基金This research was supported by National Science Foundation grants IBN-9808904 （M,R. and J.M.） and IOS-0726178 （M.R. and M.T.） the American Society of Plant Biologists＇ Education Foundation （M.R. and M.T.）+1 种基金 Ministry for Food, Agriculture, Forestry, and Fisheries, Korean Government, Korea Research Foundation （J.L.） the National Institutes of Health （grant R01ES013679 to D.B.）, and the University of Maine （M.R.）. This is Maine Agricultural and Forest Experiment Station Publication Number 3079, Hatch Project no. ME08361-08MRF （NC 1168）.ACKNOWLEDGMENTS The authors thank Dr Michael Salvucci for providing antibodies to PRK and Dr Jorn Petersen for analyzing the genomic PRK sequence for introns. No conflict of interest declared.
文摘Phosphoribulokinase （PRK）, a nuclear-encoded plastid-localized enzyme unique to the photosynthetic carbon reduction （Calvin） cycle, was cloned and characterized from the stramenopile alga Vaucheria litorea. This alga is the source of plastids for the mollusc （sea slug） Elysia chlorotica which enable the animal to survive for months solely by photoautotrophic CO2 fixation. The 1633-bp V. litorea prk gene was cloned and the coding region, found to be interrupted by four introns, encodes a 405-amino acid protein. This protein contains the typical bipartite target sequence expected of nuclearencoded proteins that are directed to complex （i.e. four membrane-bound） algal plastids. De novo synthesis of PRK and enzyme activity were detected in E. chlorotica in spite of having been starved of V. litorea for several months. Unlike the algal enzyme, PRK in the sea slug did not exhibit redox regulation. Two copies of partial PRK-encoding genes were isolated from both sea slug and aposymbiotic sea slug egg DNA using PCR. Each copy contains the nucleotide region spanning exon 1 and part of exon 2 of V litorea prk, including the bipartite targeting peptide. However, the larger prk fragment also includes intron 1. The exon and intron sequences of prk in E. chlorotica and V/itorea are nearly identical. These data suggest that PRK is differentially regulated in V. litorea and E. chlorotica and at least a portion of the V. litorea nuclear PRK gene is present in sea slugs that have been starved for several months.
文摘英语有句俗语＂My cup of tea＂,用更接地气的普通话该说成是＂我的菜＂。要时尚低调又要显出品位,你的这些挑剔口味Calvin Klein不仅全都满足,还力邀金宇彬来呈现这道新品大餐。新系列主题＂Life in the now＂讲述＂尽在此刻＂的故事,而BAZAAR Jewelry也不忘给你送上新品腕表,看看他们到底是你的茶还是你的菜？
基金financially supported by the National Natural Science Foundation of China(Nos.52379048 and 52079112)the Key Research and Development Program of Shaanxi Province,China(No.2022ZDLNY03-03)the Major Science and Technology Engineering Innovation Project of Shandong Province,China(No.2020CXGC 010808)。
文摘Aerated drip irrigation(ADI)is an important practice for promoting soil fertility and crop productivity in greenhouse vegetable production,yet little research has comprehensively investigated its effects on the functional traits of carbon(C)-cycling microorganisms.In this study,we sought to assess the potential efficacy of ADI in increasing soil organic C(SOC)by changing soil microbial communities and the expressions of genes associated with C cycling.To this end,we adopted a metagenomic approach to compare the effects of ADI with three dissolved oxygen concentrations(10,15,and 20 mg L^(-1))during a three-season tomato cultivation experiment in northern China.The results revealed that the 10 mg L^(-1)treatment led to a significant increase in the abundance of korA/B genes(associated with the reductive tricarboxylic acid cycle)in the C fixation pathway,whereas the 15 mg L^(-1)treatment increased the abundances of cbbL/R and coxL/S genes associated with the Calvin cycle and carbon monoxide oxidation,respectively.In addition,based on a co-occurrence network analysis,we observed a positive correlation between cbbL and coxS.Interestingly,r-selected microorganisms,such as Proteobacteria and Actinobacteria,characterized by rapid cell multiplication and high biomass production,were identified as the primary contributors to C fixation and were the main predictors of SOC pools.In contrast,the 20 mg L^(-1)treatment was found to adversely influence C fixation,although the enhanced C degradation could be attributed to the extracellular enzymes secreted by K-selected microorganisms.Collectively,our findings indicate that ADI with dissolved oxygen concentrations 15 mg L^(-1)can promote SOC content by altering the life history strategies of r-selected microorganisms and genes associated with C fixation.These findings will provide valuable references for agroecosystem irrigation management,help improve soil fertility,and promote sustainable production.
