Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(...Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(CRC).Methods We constructed a protein-protein interaction network of C8orf76-related genes and analyzed the prognostic signatures of C8orf76 and CDK4.Clinicopathological features of C8orf76 and CDK4 were visualized using a nomogram.Results C8orf76 and CDK4 levels were positively correlated in two independent human CRC cohorts(n=83 and n=597).A consistent positive correlation was observed between C8orf76 and CDK4 expression in the CRC cell lines.The nomogram included prognostic genes(C8orf76 and CDK4)and pathological N and M stages.The concordance index(C-index)in our cohort was 0.776,which suggests that the ability of the indicators to predict the overall survival of patients with CRC in our cohort was strong.Conclusion We found that C8orf76 was positively correlated with CDK4 in both the cohorts as well as in CRC cell lines.Therefore,C8orf76 and CDK4 can be used as potential biomarkers to predict the prognosis of CRC.展开更多
基金supported by a grant from National Natural Science Foundation of China(No.82203623)2022&2023 Hebei introduction of foreign expert intelligence projects(Nos.YZ202201&YZ202305)+1 种基金Hebei Natural Science Foundation(Nos.H2020206374 and H2021206306)Hebei Clinical Medicine Excellent Talents Project of Province(No.LS202001).
文摘Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(CRC).Methods We constructed a protein-protein interaction network of C8orf76-related genes and analyzed the prognostic signatures of C8orf76 and CDK4.Clinicopathological features of C8orf76 and CDK4 were visualized using a nomogram.Results C8orf76 and CDK4 levels were positively correlated in two independent human CRC cohorts(n=83 and n=597).A consistent positive correlation was observed between C8orf76 and CDK4 expression in the CRC cell lines.The nomogram included prognostic genes(C8orf76 and CDK4)and pathological N and M stages.The concordance index(C-index)in our cohort was 0.776,which suggests that the ability of the indicators to predict the overall survival of patients with CRC in our cohort was strong.Conclusion We found that C8orf76 was positively correlated with CDK4 in both the cohorts as well as in CRC cell lines.Therefore,C8orf76 and CDK4 can be used as potential biomarkers to predict the prognosis of CRC.
