BACKGROUND Bladder cancer(BLCA)is a common urological tumor.Homeobox C6(HOXC6)is an HOX family gene that has an oncogenic effect in various malignancies.AIM To investigate the expression and function of HOXC6 in BLCA....BACKGROUND Bladder cancer(BLCA)is a common urological tumor.Homeobox C6(HOXC6)is an HOX family gene that has an oncogenic effect in various malignancies.AIM To investigate the expression and function of HOXC6 in BLCA.METHODS This study employed immunohistochemistry,along with global chip and sequencing data for BLCA,to comprehensively evaluate the protein and mRNA expression of HOXC6 in BLCA.RNA interference technology was employed to knock down the mRNA expression of HOXC6 in BLCA cells,and the impact of reduced HOXC6 expression on cellular function was assessed.Additionally,we explored the potential mechanisms of HOXC6 in BLCA by aggregating HOXC6 chromatin immunoprecipitation sequencing data.RESULTS The immunohistochemistry results,sequencing data,and microarray data revealed that both the mRNA and protein expressions of HOXC6 in BLCA were notably greater than the expressions in non-cancerous tissues.Knocking down the expression of HOXC6 considerably limited the function of cell proliferation,migration,and invasion abilities of BLCA cells,elevated cell apoptosis,and triggered the G0/G1 phase blockade.The potential target genes of HOXC6 were enriched in pathways such as chemical carcinogenesis and reactive oxygen species.A notable positive correlation between HOXC6 mRNA expression and its target gene timeless circadian regulator(TIMELESS)was revealed.Notable binding peak signals for HOXC6 were identified in the promoter region of TIMELESS.CONCLUSION HOXC6 is upregulated in BLCA and may influence the cellular functions of BLCA by regulating the expression of the target gene TIMELESS.展开更多
M23C6 chromium-rich carbides are common grain-boundary precipitations in Cr-containing steel.The presence of grain-boundary carbides often leads to intergranular brittleness and decreases mechanical properties.This st...M23C6 chromium-rich carbides are common grain-boundary precipitations in Cr-containing steel.The presence of grain-boundary carbides often leads to intergranular brittleness and decreases mechanical properties.This study proposes a deformation and aging technique to obtain a high-volume-fraction dispersion distribution of the hard nano-M23C6 phase by changing the nucleation sites from grain boundaries to deformation coherent twin boundaries produced during cold deformation.The M23C6 precipitation-strengthened austenitic stainless steel has a strength of up to 1.4 GPa but maintains favorable plasticity(>11%).This study provides a novel approach for the control of intergranular brittleness in metallic materials.展开更多
目的探究老年慢性心力衰竭(CHF)患者G蛋白偶联受体C家族6组A亚型(GPRC6A)基因多态性及其表达与肺部感染的相关性。方法选取2021年1月~2024年1月咸阳市第一人民医院收治的138例老年CHF患者作为研究对象,根据肺部感染情况分为感染组(n=42...目的探究老年慢性心力衰竭(CHF)患者G蛋白偶联受体C家族6组A亚型(GPRC6A)基因多态性及其表达与肺部感染的相关性。方法选取2021年1月~2024年1月咸阳市第一人民医院收治的138例老年CHF患者作为研究对象,根据肺部感染情况分为感染组(n=42)和未感染组(n=96)。应用聚合酶链反应(PCR)法扩增目标区域后再通过凝胶成像系统分析GPRC6A基因rs6901250和rs1606365位点的多态性;比较感染组和未感染组等位基因和基因型频率分布差异;Logistic回归模型分析rs6901250和rs1606365位点在三种遗传模型下(共显性、显性和隐性)与老年CHF患者肺部感染的关联性;实时荧光定量PCR(RT-qPCR)法检测GPRC6A基因的表达水平;应用受试者工作特征(ROC)曲线分析GPRC6A基因的mRNA表达水平对老年CHF患者发生肺部感染的预测价值。结果GPRC6A基因rs6901250和rs1606365位点的基因型在老年CHF患者肺部感染组和未感染组的分布均符合Hardy-Weinberg平衡定律(χ^(2)=0.199~0.376,均P>0.05),具有群体代表性。与未感染组相比,感染组rs6901250位点等位基因A频率(57.14%vs 41.67%)明显升高,rs1606365位点等位基因G(54.76%vs 37.50%)和基因型GG(29.99%vs 14.06%)频率明显升高,差异具有统计学意义(χ^(2)=5.628,7.114,6.849,均P<0.05);rs6901250位点在共显性模型(GG vs AA)、显性模型(GA+AA vs GG)下,AA基因型老年CHF患者肺部感染风险均高于GG基因型(OR=1.753,1.546,P<0.05)。rs1606365位点在共显性模型(CC vs GG)、显性模型(CG+GG vs CC)、隐性模型(CG+CC vs GG)三种遗传模型下,GG基因型老年CHF患者肺部感染风险均高于CC基因型(OR=1.833,1.741,0.695,均P<0.05)。老年CHF患者肺部感染组GPRC6A基因的mRNA表达水平(1.43±0.35)明显高于未感染组(1.02±0.21),差异具有统计学意义(t=8.515,P<0.001)。ROC曲线分析结果显示,GPRC6A基因表达水平预测老年CHF患者肺部感染的AUC值为0.895,截断值为1.37,敏感度和特异度分别为85.7%,66.7%。结论GPRC6A基因rs6901250位点AA基因型,rs1606365位点GG基因型会增加老年CHF患者发生肺部感染的风险。GPRC6A基因的mRNA表达水平在感染组升高,且其表达水平可作为老年CHF患者发生肺部感染的预测指标。展开更多
Objective The effect of the functionally unknown gene C6orf120 on autoimmune hepatitis was investigated on C6orf120 knockout rats(C6orf120^(-/-))and THP-1 cells.Method Six–eight-week-old C6orf120^(-/-)and wild-type(W...Objective The effect of the functionally unknown gene C6orf120 on autoimmune hepatitis was investigated on C6orf120 knockout rats(C6orf120^(-/-))and THP-1 cells.Method Six–eight-week-old C6orf120^(-/-)and wild-type(WT)SD rats were injected with Con A(16 mg/kg),and euthanized after 24 h.The sera,livers,and spleens were collected.THP-1 cells and the recombinant protein(rC6ORF120)were used to explore the mechanism in vitro.The frequency of M1 and M2 macrophages was analyzed using flow cytometry.Western blotting and PCR were used to detect macrophage polarization-associated factors.Results C6orf120 knockout attenuated Con A-induced autoimmune hepatitis.Flow cytometry indicated that the proportion of CD68^(+)CD86^(+)M1 macrophages from the liver and spleen in the C6orf120^(-/-)rats decreased.C6orf120 knockout induced downregulation of CD86 protein and the mRNA levels of related inflammatory factors TNF-α,IL-1β,and IL-6 in the liver.C6orf120 knockout did not affect the polarization of THP-1 cells.However,rC6ORF120 promoted the THP-1 cells toward CD68^(+)CD80^(+)M1 macrophages and inhibited the CD68^(+)CD206^(+)M2 phenotype.Conclusion C6orf120 knockout alleviates Con A-induced autoimmune hepatitis by inhibiting macrophage polarization toward M1 macrophages and reducing the expression of related inflammatory factors in C6orf120^(-/-)rats.展开更多
基金Supported by the Science and Technology Major Project of Guangxi,No.AA22096030 and No.AA22096032the Yongjiang Program of Nanning+3 种基金the Creative Research Development from The First Affiliated Hospital of Guangxi Medical University,No.Medical Excellence Award 202303Guangxi Medical University Undergraduate Education and Teaching Reform Project,No.2024XJGYC20Guangxi Medical University Student Innovation and Entrepreneurship Training Program Project,No.202310598002X,No.202310598041,No.202410598039X,and No.202410598044the“Future Academic Star”of the Guangxi Medical University,No.WLXSZX24096.
文摘BACKGROUND Bladder cancer(BLCA)is a common urological tumor.Homeobox C6(HOXC6)is an HOX family gene that has an oncogenic effect in various malignancies.AIM To investigate the expression and function of HOXC6 in BLCA.METHODS This study employed immunohistochemistry,along with global chip and sequencing data for BLCA,to comprehensively evaluate the protein and mRNA expression of HOXC6 in BLCA.RNA interference technology was employed to knock down the mRNA expression of HOXC6 in BLCA cells,and the impact of reduced HOXC6 expression on cellular function was assessed.Additionally,we explored the potential mechanisms of HOXC6 in BLCA by aggregating HOXC6 chromatin immunoprecipitation sequencing data.RESULTS The immunohistochemistry results,sequencing data,and microarray data revealed that both the mRNA and protein expressions of HOXC6 in BLCA were notably greater than the expressions in non-cancerous tissues.Knocking down the expression of HOXC6 considerably limited the function of cell proliferation,migration,and invasion abilities of BLCA cells,elevated cell apoptosis,and triggered the G0/G1 phase blockade.The potential target genes of HOXC6 were enriched in pathways such as chemical carcinogenesis and reactive oxygen species.A notable positive correlation between HOXC6 mRNA expression and its target gene timeless circadian regulator(TIMELESS)was revealed.Notable binding peak signals for HOXC6 were identified in the promoter region of TIMELESS.CONCLUSION HOXC6 is upregulated in BLCA and may influence the cellular functions of BLCA by regulating the expression of the target gene TIMELESS.
基金supported by the National Natural Science Foundation of China(No.52250130).
文摘M23C6 chromium-rich carbides are common grain-boundary precipitations in Cr-containing steel.The presence of grain-boundary carbides often leads to intergranular brittleness and decreases mechanical properties.This study proposes a deformation and aging technique to obtain a high-volume-fraction dispersion distribution of the hard nano-M23C6 phase by changing the nucleation sites from grain boundaries to deformation coherent twin boundaries produced during cold deformation.The M23C6 precipitation-strengthened austenitic stainless steel has a strength of up to 1.4 GPa but maintains favorable plasticity(>11%).This study provides a novel approach for the control of intergranular brittleness in metallic materials.
文摘目的探究老年慢性心力衰竭(CHF)患者G蛋白偶联受体C家族6组A亚型(GPRC6A)基因多态性及其表达与肺部感染的相关性。方法选取2021年1月~2024年1月咸阳市第一人民医院收治的138例老年CHF患者作为研究对象,根据肺部感染情况分为感染组(n=42)和未感染组(n=96)。应用聚合酶链反应(PCR)法扩增目标区域后再通过凝胶成像系统分析GPRC6A基因rs6901250和rs1606365位点的多态性;比较感染组和未感染组等位基因和基因型频率分布差异;Logistic回归模型分析rs6901250和rs1606365位点在三种遗传模型下(共显性、显性和隐性)与老年CHF患者肺部感染的关联性;实时荧光定量PCR(RT-qPCR)法检测GPRC6A基因的表达水平;应用受试者工作特征(ROC)曲线分析GPRC6A基因的mRNA表达水平对老年CHF患者发生肺部感染的预测价值。结果GPRC6A基因rs6901250和rs1606365位点的基因型在老年CHF患者肺部感染组和未感染组的分布均符合Hardy-Weinberg平衡定律(χ^(2)=0.199~0.376,均P>0.05),具有群体代表性。与未感染组相比,感染组rs6901250位点等位基因A频率(57.14%vs 41.67%)明显升高,rs1606365位点等位基因G(54.76%vs 37.50%)和基因型GG(29.99%vs 14.06%)频率明显升高,差异具有统计学意义(χ^(2)=5.628,7.114,6.849,均P<0.05);rs6901250位点在共显性模型(GG vs AA)、显性模型(GA+AA vs GG)下,AA基因型老年CHF患者肺部感染风险均高于GG基因型(OR=1.753,1.546,P<0.05)。rs1606365位点在共显性模型(CC vs GG)、显性模型(CG+GG vs CC)、隐性模型(CG+CC vs GG)三种遗传模型下,GG基因型老年CHF患者肺部感染风险均高于CC基因型(OR=1.833,1.741,0.695,均P<0.05)。老年CHF患者肺部感染组GPRC6A基因的mRNA表达水平(1.43±0.35)明显高于未感染组(1.02±0.21),差异具有统计学意义(t=8.515,P<0.001)。ROC曲线分析结果显示,GPRC6A基因表达水平预测老年CHF患者肺部感染的AUC值为0.895,截断值为1.37,敏感度和特异度分别为85.7%,66.7%。结论GPRC6A基因rs6901250位点AA基因型,rs1606365位点GG基因型会增加老年CHF患者发生肺部感染的风险。GPRC6A基因的mRNA表达水平在感染组升高,且其表达水平可作为老年CHF患者发生肺部感染的预测指标。
基金supported by the Dengfeng Talent Support Program of Beijing Municipal Administration of Hospitals[Grant No.DFL20221601]the Natural Science Foundation of Beijing[Grant No.7212053]Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine[Grant No.ZYYCXTD-C-202006].
文摘Objective The effect of the functionally unknown gene C6orf120 on autoimmune hepatitis was investigated on C6orf120 knockout rats(C6orf120^(-/-))and THP-1 cells.Method Six–eight-week-old C6orf120^(-/-)and wild-type(WT)SD rats were injected with Con A(16 mg/kg),and euthanized after 24 h.The sera,livers,and spleens were collected.THP-1 cells and the recombinant protein(rC6ORF120)were used to explore the mechanism in vitro.The frequency of M1 and M2 macrophages was analyzed using flow cytometry.Western blotting and PCR were used to detect macrophage polarization-associated factors.Results C6orf120 knockout attenuated Con A-induced autoimmune hepatitis.Flow cytometry indicated that the proportion of CD68^(+)CD86^(+)M1 macrophages from the liver and spleen in the C6orf120^(-/-)rats decreased.C6orf120 knockout induced downregulation of CD86 protein and the mRNA levels of related inflammatory factors TNF-α,IL-1β,and IL-6 in the liver.C6orf120 knockout did not affect the polarization of THP-1 cells.However,rC6ORF120 promoted the THP-1 cells toward CD68^(+)CD80^(+)M1 macrophages and inhibited the CD68^(+)CD206^(+)M2 phenotype.Conclusion C6orf120 knockout alleviates Con A-induced autoimmune hepatitis by inhibiting macrophage polarization toward M1 macrophages and reducing the expression of related inflammatory factors in C6orf120^(-/-)rats.
