针对背景复杂的变电站电力设备过热故障红外图像难以检测的问题,提出了改进你只看一次11纳米版(you only look once version 11 nano, YOLOv11n)算法。首先,采用轻量级跨尺度特征融合模块(cross-scale feature fusion module, CCFM)改...针对背景复杂的变电站电力设备过热故障红外图像难以检测的问题,提出了改进你只看一次11纳米版(you only look once version 11 nano, YOLOv11n)算法。首先,采用轻量级跨尺度特征融合模块(cross-scale feature fusion module, CCFM)改进原有颈部网络,以实现对特征通道信息的高效整合并降低参数量;其次,引入具有可切换空洞卷积的2次跨阶段3卷积可变核(cross stage partial with three-convolution blocks of variable kernel size two-switchable atrous convolution, C3k2-SAConv)模块替换整个网络的C3k2模块,提升了算法的特征提取能力;最后,使用具有双层路由注意力视觉转换器的跨阶段双卷积(cross stage partial with two convolutions and vision transformer of bi-level routing attention, C2BF)模块替换跨阶段双卷积逐点空间注意力(cross stage partial with two convolutions and pointwise spatial attention, C2PSA)模块,提升了算法在复杂环境下对红外图像的检测准确度。结果表明,相较于原始YOLOv11n算法,改进YOLOv11n算法的参数量减少了22.1%;精确率、召回率、平均精确率均值分别达到91.1%、85.5%、90.9%,各自提升了3.0、2.6、2.8个百分点;检测速度达到128.2帧/s。改进YOLOv11n算法能实现对电力设备过热故障红外图像的有效检测,可满足算法轻量化与实时性检测的要求。展开更多
针对目前人工巡视导致的变电站设备及生产行为异常检测效率低、人工风险高等问题,提出改进你只看一次11纳米版(you only look once version 11 nano, YOLOv11n)模型。首先,通过设计基于自注意力机制的3尺度卷积双路径可变核(convolution...针对目前人工巡视导致的变电站设备及生产行为异常检测效率低、人工风险高等问题,提出改进你只看一次11纳米版(you only look once version 11 nano, YOLOv11n)模型。首先,通过设计基于自注意力机制的3尺度卷积双路径可变核(convolutional three-scale kernel-adaptive dual-path self-attention mechanism, C3k2-SA)模块在较小特征图衔接特征融合部分,优化了网络结构,增强了全局特征提取能力。然后,在主干网络末层引入了基于注意力机制的特征增强(feature enhancement, FEN)模块,动态调整不同区域的特征权重,实现自适应的特征增强,缓解深层网络中的梯度消失问题。最后,对拼接(concatenate, Concat)模块进行优化,通过卷积层调整通道数,采用池化和sigmoid激活函数进行特征的精细处理,提高了模型对不同类型特征的自适应,增强了特征融合效果,同时抑制了无关或冗余特征,防止过拟合。结果表明,与原始YOLOv11n模型相比,改进YOLOv11n模型的精确率、召回率、平均精确率均值分别上升了1.7、6.6、3.6个百分点。改进YOLOv11n模型能够提高变电站异常状态检测的准确性,为智能变电站的异常检测工作提供一定参考。展开更多
[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌...[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌细胞增殖能力,Transwell实验和划痕实验分析宫颈癌细胞侵袭和迁移能力,流式细胞术检测宫颈癌细胞的凋亡率,蛋白免疫印迹分析OR13C2对PI3K/AKT通路蛋白表达的影响。[结果]OR13C2在宫颈癌组织中低表达,而在宫颈癌旁组织中高表达(0.23±0.02 vs 0.97±0.07;2.01±0.05 vs 4.81±0.13;P<0.05)。OR13C2高表达后能够抑制宫颈癌细胞的增殖、侵袭和迁移能力,促进宫颈癌细胞凋亡。此外,OR13C2高表达能够抑制PI3K/AKT的激活(0.73±0.03 vs 0.19±0.05 vs 0.26±0.08;0.72±0.08 vs 0.23±0.06 vs 0.31±0.03;P<0.05)。[结论]OR13C2在宫颈癌组织中表达减少,上调OR13C2的表达可以抑制宫颈癌HeLa细胞的增殖、侵袭和迁移能力,促进宫颈癌HeLa细胞凋亡,此作用与OR13C2抑制PI3K/AKT通路的激活相关。展开更多
Spermatogenesis is a fundamental process that requires a tightly controlled epigenetic event in spermatogonial stem cells(SSCs).The mechanisms underlying the transition from SSCs to sperm are largely unknown.Most stud...Spermatogenesis is a fundamental process that requires a tightly controlled epigenetic event in spermatogonial stem cells(SSCs).The mechanisms underlying the transition from SSCs to sperm are largely unknown.Most studies utilize gene knockout mice to explain the mechanisms.However,the production of genetically engineered mice is costly and time-consuming.In this study,we presented a convenient research strategy using an RNA interference(RNAi)and testicular transplantation approach.Histone H3 lysine 9(H3K9)methylation was dynamically regulated during spermatogenesis.As Jumonji domain-containing protein 1A(JMJD1A)and Jumonji domain-containing protein 2C(JMJD2C)demethylases catalyze histone H3 lysine 9 dimethylation(H3K9me2),we firstly analyzed the expression profile of the two demethylases and then investigated their function.Using the convenient research strategy,we showed that normal spermatogenesis is disrupted due to the downregulated expression of both demethylases.These results suggest that this strategy might be a simple and alternative approach for analyzing spermatogenesis relative to the gene knockout mice strategy.展开更多
文摘针对背景复杂的变电站电力设备过热故障红外图像难以检测的问题,提出了改进你只看一次11纳米版(you only look once version 11 nano, YOLOv11n)算法。首先,采用轻量级跨尺度特征融合模块(cross-scale feature fusion module, CCFM)改进原有颈部网络,以实现对特征通道信息的高效整合并降低参数量;其次,引入具有可切换空洞卷积的2次跨阶段3卷积可变核(cross stage partial with three-convolution blocks of variable kernel size two-switchable atrous convolution, C3k2-SAConv)模块替换整个网络的C3k2模块,提升了算法的特征提取能力;最后,使用具有双层路由注意力视觉转换器的跨阶段双卷积(cross stage partial with two convolutions and vision transformer of bi-level routing attention, C2BF)模块替换跨阶段双卷积逐点空间注意力(cross stage partial with two convolutions and pointwise spatial attention, C2PSA)模块,提升了算法在复杂环境下对红外图像的检测准确度。结果表明,相较于原始YOLOv11n算法,改进YOLOv11n算法的参数量减少了22.1%;精确率、召回率、平均精确率均值分别达到91.1%、85.5%、90.9%,各自提升了3.0、2.6、2.8个百分点;检测速度达到128.2帧/s。改进YOLOv11n算法能实现对电力设备过热故障红外图像的有效检测,可满足算法轻量化与实时性检测的要求。
文摘[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌细胞增殖能力,Transwell实验和划痕实验分析宫颈癌细胞侵袭和迁移能力,流式细胞术检测宫颈癌细胞的凋亡率,蛋白免疫印迹分析OR13C2对PI3K/AKT通路蛋白表达的影响。[结果]OR13C2在宫颈癌组织中低表达,而在宫颈癌旁组织中高表达(0.23±0.02 vs 0.97±0.07;2.01±0.05 vs 4.81±0.13;P<0.05)。OR13C2高表达后能够抑制宫颈癌细胞的增殖、侵袭和迁移能力,促进宫颈癌细胞凋亡。此外,OR13C2高表达能够抑制PI3K/AKT的激活(0.73±0.03 vs 0.19±0.05 vs 0.26±0.08;0.72±0.08 vs 0.23±0.06 vs 0.31±0.03;P<0.05)。[结论]OR13C2在宫颈癌组织中表达减少,上调OR13C2的表达可以抑制宫颈癌HeLa细胞的增殖、侵袭和迁移能力,促进宫颈癌HeLa细胞凋亡,此作用与OR13C2抑制PI3K/AKT通路的激活相关。
基金financially supported by the Shandong Provincial Natural Science Foundation(No.ZR2021QC182).
文摘Spermatogenesis is a fundamental process that requires a tightly controlled epigenetic event in spermatogonial stem cells(SSCs).The mechanisms underlying the transition from SSCs to sperm are largely unknown.Most studies utilize gene knockout mice to explain the mechanisms.However,the production of genetically engineered mice is costly and time-consuming.In this study,we presented a convenient research strategy using an RNA interference(RNAi)and testicular transplantation approach.Histone H3 lysine 9(H3K9)methylation was dynamically regulated during spermatogenesis.As Jumonji domain-containing protein 1A(JMJD1A)and Jumonji domain-containing protein 2C(JMJD2C)demethylases catalyze histone H3 lysine 9 dimethylation(H3K9me2),we firstly analyzed the expression profile of the two demethylases and then investigated their function.Using the convenient research strategy,we showed that normal spermatogenesis is disrupted due to the downregulated expression of both demethylases.These results suggest that this strategy might be a simple and alternative approach for analyzing spermatogenesis relative to the gene knockout mice strategy.
