A gradient reversed-phase high-performance liquid chromatography(HPLC)method using a C30 col-umn was developed for the simultaneous determination of astaxanthin,astaxanthin monoesters and astaxanthin diesters in the g...A gradient reversed-phase high-performance liquid chromatography(HPLC)method using a C30 col-umn was developed for the simultaneous determination of astaxanthin,astaxanthin monoesters and astaxanthin diesters in the green algae Chlorococcum sp.,Chlorella zofingiensis,Haematococcus plu-vialis and the mutant E1,which was obtained from the mutagenesis of H.pluvialis by exposure to UV-irradiation and ethyl methanesulphonate(EMS)with subsequent screening using nicotine.The re-sults showed that the contents of total astaxanthins including free astaxanthin and astaxanthin esters ranged from 1.4 to 30.9 mg/g dry biomass in these green algae.The lower total astaxanthin levels(<2 mg/g dry biomass)were detected in the green algae Chlorococcum sp.and C.zofingiensis.The higher total astaxanthin levels(>16 mg/g dry biomass)were found in the green alga H.pluvialis and its mutant E1.It is notable that the mutant E1 is found to have considerably higher amounts of total astaxanthin(30.9 mg/g)as compared to the wild strain of H.pluvialis(16.1 mg/g).This indicates that UV-irradiation and EMS compound mutagenesis with subsequent screening using nicotine is an effective method for breeding of a high-producing astaxanthin strain of H.pluvialis.In addition,the green alga C.zofingien-sis had a remarkably higher percentage of astaxanthin diesters(76.3%of total astaxanthins)and a re-markably lower percentage of astaxanthin monoesters(18.0%of total astaxanthins)in comparison with H.pluvialis(35.5%for diesters and 60.9%for monoesters),the mutant E1(49.1%and 48.1%)and Chlorococcum sp.(18.0%and 58.6%).展开更多
基金Supported by the Frontier Research Grant of the SCSIOthe Hundred Talents program of Chinese Academy of SciencesNational Natural Sciences of China projects(Grant No.40776087)
文摘A gradient reversed-phase high-performance liquid chromatography(HPLC)method using a C30 col-umn was developed for the simultaneous determination of astaxanthin,astaxanthin monoesters and astaxanthin diesters in the green algae Chlorococcum sp.,Chlorella zofingiensis,Haematococcus plu-vialis and the mutant E1,which was obtained from the mutagenesis of H.pluvialis by exposure to UV-irradiation and ethyl methanesulphonate(EMS)with subsequent screening using nicotine.The re-sults showed that the contents of total astaxanthins including free astaxanthin and astaxanthin esters ranged from 1.4 to 30.9 mg/g dry biomass in these green algae.The lower total astaxanthin levels(<2 mg/g dry biomass)were detected in the green algae Chlorococcum sp.and C.zofingiensis.The higher total astaxanthin levels(>16 mg/g dry biomass)were found in the green alga H.pluvialis and its mutant E1.It is notable that the mutant E1 is found to have considerably higher amounts of total astaxanthin(30.9 mg/g)as compared to the wild strain of H.pluvialis(16.1 mg/g).This indicates that UV-irradiation and EMS compound mutagenesis with subsequent screening using nicotine is an effective method for breeding of a high-producing astaxanthin strain of H.pluvialis.In addition,the green alga C.zofingien-sis had a remarkably higher percentage of astaxanthin diesters(76.3%of total astaxanthins)and a re-markably lower percentage of astaxanthin monoesters(18.0%of total astaxanthins)in comparison with H.pluvialis(35.5%for diesters and 60.9%for monoesters),the mutant E1(49.1%and 48.1%)and Chlorococcum sp.(18.0%and 58.6%).
