目的:研究银杏酚C15∶1与DNA的相互作用情况。方法:吖啶橙(AO)为荧光探针,在293 K和310 K p H7.4的Tris-HCl缓冲液中,采用荧光光谱法、粘度法和热溶解实验研究银杏酚C15∶1和鲱鱼精DNA的相互作用方式。结果:银杏酚C15∶1与AO-DNA之间的...目的:研究银杏酚C15∶1与DNA的相互作用情况。方法:吖啶橙(AO)为荧光探针,在293 K和310 K p H7.4的Tris-HCl缓冲液中,采用荧光光谱法、粘度法和热溶解实验研究银杏酚C15∶1和鲱鱼精DNA的相互作用方式。结果:银杏酚C15∶1与AO-DNA之间的猝灭方式为静态猝灭,根据热力学参数确定作用力类型是以氢键作用为主,判断银杏酚C15∶1与AO-DNA之间的作用方式主要是嵌插作用。结论:粘度法及热变性实验结果进一步证明银杏酚C15∶1与AO-DNA之间的主要作用方式是嵌插模式。展开更多
Molecular dynamics simulations are used to investigate the stabilities of C15 Laves phase structures subjected to temperature and point defects. The simulations based on different empirical potentials show that the bu...Molecular dynamics simulations are used to investigate the stabilities of C15 Laves phase structures subjected to temperature and point defects. The simulations based on different empirical potentials show that the bulk perfect C15 Laves phase appears to be stable under a critical temperature in a range from 350 K to 450 K, beyond which it becomes disordered and experiences an abrupt decrement of elastic modulus. In the presence of both vacancy and self-interstitial, the bulk C15 Laves phase becomes unstable at room temperature and prefers to transform into an imperfect body centered cubic（BCC）structure containing free vacancies or vacancy clusters. When a C15 cluster is embedded in BCC iron, the annihilation of interstitials occurs due to the presence of the vacancy, while it exhibits a phase transformation into a（1/2） 111 dislocation loop due to the presence of the self-interstitial.展开更多
文摘目的:研究银杏酚C15∶1与DNA的相互作用情况。方法:吖啶橙(AO)为荧光探针,在293 K和310 K p H7.4的Tris-HCl缓冲液中,采用荧光光谱法、粘度法和热溶解实验研究银杏酚C15∶1和鲱鱼精DNA的相互作用方式。结果:银杏酚C15∶1与AO-DNA之间的猝灭方式为静态猝灭,根据热力学参数确定作用力类型是以氢键作用为主,判断银杏酚C15∶1与AO-DNA之间的作用方式主要是嵌插作用。结论:粘度法及热变性实验结果进一步证明银杏酚C15∶1与AO-DNA之间的主要作用方式是嵌插模式。
基金supported by the National Natural Science Foundation of China(Grant Nos.11675230 and 11375242)Canada’s Natural Sciences and Engineering Research Council NSERC Discovery GrantCanada Foundation for Innovation CFI
文摘Molecular dynamics simulations are used to investigate the stabilities of C15 Laves phase structures subjected to temperature and point defects. The simulations based on different empirical potentials show that the bulk perfect C15 Laves phase appears to be stable under a critical temperature in a range from 350 K to 450 K, beyond which it becomes disordered and experiences an abrupt decrement of elastic modulus. In the presence of both vacancy and self-interstitial, the bulk C15 Laves phase becomes unstable at room temperature and prefers to transform into an imperfect body centered cubic（BCC）structure containing free vacancies or vacancy clusters. When a C15 cluster is embedded in BCC iron, the annihilation of interstitials occurs due to the presence of the vacancy, while it exhibits a phase transformation into a（1/2） 111 dislocation loop due to the presence of the self-interstitial.
