Chroogomphis rutilus is a kind of delicious edible fungus rich in bioactive components such as polysaccharides,and has high nutritional values.However,the researches on the structure-activity relationship of polysacch...Chroogomphis rutilus is a kind of delicious edible fungus rich in bioactive components such as polysaccharides,and has high nutritional values.However,the researches on the structure-activity relationship of polysaccharide components are still ambiguous.In this study,the C.rutilus water-extracted polysaccharide(CRWP)and alkali-extracted polysaccharide(CRAP)were obtained using deionized water and weak alkali solutions,respectively,and purified by dialysis,and the gut microbiota mediated immune regulatory activities were further studied.Structural analysis results showed that both CRWP and CRAP exhibited typical polysaccharide characteristics,showing the main chains ofβ-(1→6)-Manp andβ-(1→6)-Glcp linkedβ-(1→6)-Manp,respectively,with the molecular weights of 1.91×10^(7) Da and 2.19×10^(7) Da.In cyclophosphamide induced immune-injured mice,CRWP and CRAP effectively regulated the Lactobacillaceae/Lactobacillus mediated plant hormone signal trans-duction pathway and Akkermansiacae/Akkermansia mediated lysine degradation pathway,respectively.Besides,CRWP exhibited superior immunomodulatory effects on the proportions of CD19+B cells and CD3+T cells in peripheral blood compared with CRAP.These findings provide experimental data and theoretical basis for the development and application of C.rutilus polysaccharides in functional food fields.展开更多
基金supported by the Natural Science Foundation of Tianjin(No.24JCYBJC00440).
文摘Chroogomphis rutilus is a kind of delicious edible fungus rich in bioactive components such as polysaccharides,and has high nutritional values.However,the researches on the structure-activity relationship of polysaccharide components are still ambiguous.In this study,the C.rutilus water-extracted polysaccharide(CRWP)and alkali-extracted polysaccharide(CRAP)were obtained using deionized water and weak alkali solutions,respectively,and purified by dialysis,and the gut microbiota mediated immune regulatory activities were further studied.Structural analysis results showed that both CRWP and CRAP exhibited typical polysaccharide characteristics,showing the main chains ofβ-(1→6)-Manp andβ-(1→6)-Glcp linkedβ-(1→6)-Manp,respectively,with the molecular weights of 1.91×10^(7) Da and 2.19×10^(7) Da.In cyclophosphamide induced immune-injured mice,CRWP and CRAP effectively regulated the Lactobacillaceae/Lactobacillus mediated plant hormone signal trans-duction pathway and Akkermansiacae/Akkermansia mediated lysine degradation pathway,respectively.Besides,CRWP exhibited superior immunomodulatory effects on the proportions of CD19+B cells and CD3+T cells in peripheral blood compared with CRAP.These findings provide experimental data and theoretical basis for the development and application of C.rutilus polysaccharides in functional food fields.
