Background:Corchorus olitorius L.(Malvaceae)is a green leafy vegetable widely consumed in the Middle East and valued for its rich nutritional content.Its essential oils,an important class of secondary metabolites,are ...Background:Corchorus olitorius L.(Malvaceae)is a green leafy vegetable widely consumed in the Middle East and valued for its rich nutritional content.Its essential oils,an important class of secondary metabolites,are of growing interest for potential use in cosmetics and fragrance industries due to their bioactive properties.Methods:Leaves of C.olitorius cultivated in Egypt were extracted using microwave-assisted hydrodistillation(MAHD)and conventional hydrodistillation(HD).The oils were analyzed by gas chromatography-mass spectrometry(GC-MS)to characterize their chemical profiles.In vitro assays were conducted to evaluate their anti-elastase and anti-collagenase activities,and in silico studies were performed to predict the pharmacokinetic and pharmacodynamic properties of major constituents.Results:GC-MS analysis showed that aldehydes,alkanes,fatty alcohols,fatty acids,and their derivatives were the predominant compound classes in both oils.Notably,oxygenated diterpenes(11.22%)were present exclusively in the MAHD oil,while triterpenoids(16.37%)were found only in the HD oil.The MAHD oil demonstrated stronger In vitro anti-elastase(IC_(50)=42.5μg/mL)and anti-collagenase(IC_(50)=131.5μg/mL)activities compared to the HD oil(IC_(50)=66.7 and 206.8μg/mL,respectively),reflecting an approximate 36.3%improvement in elastase inhibition and 36.4%improvement in collagenase inhibition.In silico docking indicated that the triterpenoidβ-amyrin acetate showed the highest predicted binding affinity for porcine pancreatic elastase(PDB ID:6QEO,ΔG=−8.1 kcal/mol)and collagenase(PDB ID:456C,ΔG=−9.1 kcal/mol)among the major compounds analyzed.Conclusion:These findings demonstrate that MAHD is a greener and more efficient extraction method,yielding oil with enhanced enzyme inhibitory activity compared to conventional HD.The promising anti-elastase and anti-collagenase properties suggest that C.olitorius MAHD oil could serve as a potential candidate for anti-aging cosmetic formulations,following further validation.展开更多
目的探究卵巢癌组织中热休克蛋白70(heat shock protein 70,HSP70)、核分布蛋白C(nuclear distribution protein C,NUDC)与疾病进展的关系。方法选取150例卵巢癌患者(观察组)和134例非肿瘤妇科疾病患者(对照组),检测2组卵巢组织中HSP70...目的探究卵巢癌组织中热休克蛋白70(heat shock protein 70,HSP70)、核分布蛋白C(nuclear distribution protein C,NUDC)与疾病进展的关系。方法选取150例卵巢癌患者(观察组)和134例非肿瘤妇科疾病患者(对照组),检测2组卵巢组织中HSP70与NUDC的表达情况;通过单因素及多因素Logistic回归分析卵巢癌疾病进展的影响因素。结果观察组卵巢组织中HSP70与NUDC的表达水平显著高于对照组(P<0.05);Logistic回归分析显示,卵巢癌疾病进展的影响因素有临床分期、有其他器官转移、HSP70高表达、NUDC高表达(P<0.05)。结论卵巢癌组织中HSP70与NUDC的表达水平显著高于非肿瘤卵巢组织,HSP70、NUDC高表达均为卵巢癌疾病进展的影响因素。展开更多
基金Egyptian Science and Technology and Innovation Fund(STIFA)for the support of the study through grant No.46667 entitled“Sustainability of Lab Capacities of the Center of Drug Discovery Research and Development”.
文摘Background:Corchorus olitorius L.(Malvaceae)is a green leafy vegetable widely consumed in the Middle East and valued for its rich nutritional content.Its essential oils,an important class of secondary metabolites,are of growing interest for potential use in cosmetics and fragrance industries due to their bioactive properties.Methods:Leaves of C.olitorius cultivated in Egypt were extracted using microwave-assisted hydrodistillation(MAHD)and conventional hydrodistillation(HD).The oils were analyzed by gas chromatography-mass spectrometry(GC-MS)to characterize their chemical profiles.In vitro assays were conducted to evaluate their anti-elastase and anti-collagenase activities,and in silico studies were performed to predict the pharmacokinetic and pharmacodynamic properties of major constituents.Results:GC-MS analysis showed that aldehydes,alkanes,fatty alcohols,fatty acids,and their derivatives were the predominant compound classes in both oils.Notably,oxygenated diterpenes(11.22%)were present exclusively in the MAHD oil,while triterpenoids(16.37%)were found only in the HD oil.The MAHD oil demonstrated stronger In vitro anti-elastase(IC_(50)=42.5μg/mL)and anti-collagenase(IC_(50)=131.5μg/mL)activities compared to the HD oil(IC_(50)=66.7 and 206.8μg/mL,respectively),reflecting an approximate 36.3%improvement in elastase inhibition and 36.4%improvement in collagenase inhibition.In silico docking indicated that the triterpenoidβ-amyrin acetate showed the highest predicted binding affinity for porcine pancreatic elastase(PDB ID:6QEO,ΔG=−8.1 kcal/mol)and collagenase(PDB ID:456C,ΔG=−9.1 kcal/mol)among the major compounds analyzed.Conclusion:These findings demonstrate that MAHD is a greener and more efficient extraction method,yielding oil with enhanced enzyme inhibitory activity compared to conventional HD.The promising anti-elastase and anti-collagenase properties suggest that C.olitorius MAHD oil could serve as a potential candidate for anti-aging cosmetic formulations,following further validation.
文摘目的探究卵巢癌组织中热休克蛋白70(heat shock protein 70,HSP70)、核分布蛋白C(nuclear distribution protein C,NUDC)与疾病进展的关系。方法选取150例卵巢癌患者(观察组)和134例非肿瘤妇科疾病患者(对照组),检测2组卵巢组织中HSP70与NUDC的表达情况;通过单因素及多因素Logistic回归分析卵巢癌疾病进展的影响因素。结果观察组卵巢组织中HSP70与NUDC的表达水平显著高于对照组(P<0.05);Logistic回归分析显示,卵巢癌疾病进展的影响因素有临床分期、有其他器官转移、HSP70高表达、NUDC高表达(P<0.05)。结论卵巢癌组织中HSP70与NUDC的表达水平显著高于非肿瘤卵巢组织,HSP70、NUDC高表达均为卵巢癌疾病进展的影响因素。
