C-repeat binding proteins （CBFs） are a group of transcription factors that have been proven to be important for stress tolerance in plants. Many of these transcription factors transactivate the promoters of cold-reg...C-repeat binding proteins （CBFs） are a group of transcription factors that have been proven to be important for stress tolerance in plants. Many of these transcription factors transactivate the promoters of cold-regulated genes via binding to low temperature-or dehydration-responsive c/s-elements, thus conferring plants cold acclimation. In the present study, we isolated a C-repeat binding transcription factor from maize using the yeast one-hybrid system with the C-repeat motif from the promoter of the Arabidopsis COR15a gene as bait. The isolated transcription factor is highly similar to the Arabidopsis CBF3 in their predicted amino acid sequences, and is therefore designated ZmCBF3. Point mutation analyses of the ZmCBF3-binding c/s-element revealed （A/G）（C/T）CGAC as the core binding sequence. Expression analyses showed that ZmCBF3 was upregulated by both abscisic acid and low temperature, and was actively expressed during embryogenesis, suggesting that ZmCBF3 plays a role in stress response in maize.展开更多
Cold temperatures, a major abiotic stress, threaten the growth and development of plants, worldwide. To cope with this adverse environmental cue, plants from temperate climates have evolved an array of sophisticated m...Cold temperatures, a major abiotic stress, threaten the growth and development of plants, worldwide. To cope with this adverse environmental cue, plants from temperate climates have evolved an array of sophisticated mechanisms to acclimate to cold periods, increasing their ability to tolerate freezing stress. Over the last decade, significant progress has been made in determining the molecular mechanisms underpinning cold acclimation, including following the identification of several pivotal components, including candidates for cold sensors, protein kinases, and transcription factors. With these developments, we have a better understanding of the CBF-dependent cold-signaling pathway. In this review, we summarize recent progress made in elucidating the cold-signaling pathways, especially the C-repeat binding factor-dependent pathway, and describe the regulatory function of the crucial components of plant cold signaling. We also discuss the unsolved questions that should be the focus of future work.展开更多
Low temperature stress is one of the most important factors limiting plant growth and geographical distribution.In order to adapt to low temperature,plants have evolved strategies to acquire cold tolerance,known as,co...Low temperature stress is one of the most important factors limiting plant growth and geographical distribution.In order to adapt to low temperature,plants have evolved strategies to acquire cold tolerance,known as,cold acclimation.Current molecular and genomic studies have reported that annual herbaceous and perennial woody plants share similar cold acclimation mechanisms.However,woody perennials also require extra resilience to survive cold winters.Thus,trees have acquired complex dynamic processes to control the development of dormancy and cold resistance,ensuring successful tolerance during the coldest winter season.In this review,we systemically described how woody plants perceive and transduce cold stress signals through a series of physiological changes such as calcium signaling,membrane lipid,and antioxidant changes altering downstream gene expression and epigenetic modification,ultimately bud dormancy.We extended the discussion and reviewed the processes endogenous phytohormones play in regulating the cold stress.We believe that this review will aid in the comprehension of underlying mechanisms in plant acclimation to cold stress.展开更多
基金the National Natural Science Foundation of China (30671195and 30300028)
文摘C-repeat binding proteins （CBFs） are a group of transcription factors that have been proven to be important for stress tolerance in plants. Many of these transcription factors transactivate the promoters of cold-regulated genes via binding to low temperature-or dehydration-responsive c/s-elements, thus conferring plants cold acclimation. In the present study, we isolated a C-repeat binding transcription factor from maize using the yeast one-hybrid system with the C-repeat motif from the promoter of the Arabidopsis COR15a gene as bait. The isolated transcription factor is highly similar to the Arabidopsis CBF3 in their predicted amino acid sequences, and is therefore designated ZmCBF3. Point mutation analyses of the ZmCBF3-binding c/s-element revealed （A/G）（C/T）CGAC as the core binding sequence. Expression analyses showed that ZmCBF3 was upregulated by both abscisic acid and low temperature, and was actively expressed during embryogenesis, suggesting that ZmCBF3 plays a role in stress response in maize.
基金supported by grants from the National Natural Science Foundation of China(31730011 and 31700214)
文摘Cold temperatures, a major abiotic stress, threaten the growth and development of plants, worldwide. To cope with this adverse environmental cue, plants from temperate climates have evolved an array of sophisticated mechanisms to acclimate to cold periods, increasing their ability to tolerate freezing stress. Over the last decade, significant progress has been made in determining the molecular mechanisms underpinning cold acclimation, including following the identification of several pivotal components, including candidates for cold sensors, protein kinases, and transcription factors. With these developments, we have a better understanding of the CBF-dependent cold-signaling pathway. In this review, we summarize recent progress made in elucidating the cold-signaling pathways, especially the C-repeat binding factor-dependent pathway, and describe the regulatory function of the crucial components of plant cold signaling. We also discuss the unsolved questions that should be the focus of future work.
文摘目的:探讨细胞分泌蛋白1(C-type lectin repeat protein-1,CTRP1)、细胞分泌蛋白3(C-type lectin repeat protein-3,CTRP3)水平变化在评估阿替普酶+替罗非班治疗超急性期缺血性脑卒中的临床价值。方法:研究本院2021年06月至2024年01月纳入的92例超急性期缺血性脑卒中患者随机分为观察组、对照组各46例。对照组实施阿替普酶静脉溶栓治疗,观察组给予阿替普酶静脉溶栓后序贯替罗非班治疗。比较两组治疗效果,Barthel评分和美国卫生部卒中量表(National Institute of Health Stroke Scale Score,NIHSS)评分,CTRP1和CTRP3水平,血脂水平,不良事件发生率情况。结果:治疗总有效率观察组高于对照组(P<0.05)。治疗后两组Barthel评分和NIHSS评分均改善,与对照组相比观察组更优(P<0.05)。治疗后12 h、24 h两组CTRP1和CTRP3水平均依次降低,与对照组相比,观察组CTRP1和CTRP3水平更低,(P<0.05)。治疗后两组血脂水平均降低,与对照组相比,观察组各血脂水平更低(P<0.05)。观察组总不良事件发生率低于对照组(P<0.05)。结论:阿替普酶+替罗非班在超急性期缺血性脑卒中患者治疗中可有效改善患者的日常生活能力及神经功能缺损,降低患者的血脂水平,CTRP1、CTRP3水平逐渐降低,不良事件发生率较低,临床疗效显著。
基金funded by the National Natural Science Foundation of China(No.31971682)the Research Startup Fund for High-Level and High-Educated Talents of Nanjing Forestry University.
文摘Low temperature stress is one of the most important factors limiting plant growth and geographical distribution.In order to adapt to low temperature,plants have evolved strategies to acquire cold tolerance,known as,cold acclimation.Current molecular and genomic studies have reported that annual herbaceous and perennial woody plants share similar cold acclimation mechanisms.However,woody perennials also require extra resilience to survive cold winters.Thus,trees have acquired complex dynamic processes to control the development of dormancy and cold resistance,ensuring successful tolerance during the coldest winter season.In this review,we systemically described how woody plants perceive and transduce cold stress signals through a series of physiological changes such as calcium signaling,membrane lipid,and antioxidant changes altering downstream gene expression and epigenetic modification,ultimately bud dormancy.We extended the discussion and reviewed the processes endogenous phytohormones play in regulating the cold stress.We believe that this review will aid in the comprehension of underlying mechanisms in plant acclimation to cold stress.
