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A C-Band Monolithic GaAs PIN Diode SPST Switch
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作者 吴茹菲 张健 +1 位作者 尹军舰 张海英 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2008年第5期879-882,共4页
A monolithic single pole single throw (SPST) switch is developed with GaAs PIN diode technology from IMECAS. A novel small signal model of a GaAs PIN diode is developed for circuit simulation. The switch features an... A monolithic single pole single throw (SPST) switch is developed with GaAs PIN diode technology from IMECAS. A novel small signal model of a GaAs PIN diode is developed for circuit simulation. The switch features an on-state insertion loss of less than 1.6dB and a return loss of greater than 10dB while maintaining an off-state isolation of greater than 23dB from 5.5 to 7. 5GHz. The measured 1dB power gain compression point is about 20dBm. 展开更多
关键词 c-band SPST switches OaAs PIN diodes
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药用植物丹参的核型分析和C-banding研究 被引量:4
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作者 唐晓清 吴健 +2 位作者 余伯阳 王康才 别同德 《江西农业学报》 CAS 2006年第3期75-77,85,共4页
采用传统的核型分析技术,分析了栽培丹参有丝分裂中期染色体的核型和C—banding。结果表明:丹参中期染色体长度为1.30-2.39μm,核型公式是2n=16=4m+2sm+2st,其核型为2A型;单套染色体组显示出19条清晰稳定的C—bands,其中包括1... 采用传统的核型分析技术,分析了栽培丹参有丝分裂中期染色体的核型和C—banding。结果表明:丹参中期染色体长度为1.30-2.39μm,核型公式是2n=16=4m+2sm+2st,其核型为2A型;单套染色体组显示出19条清晰稳定的C—bands,其中包括13条末端带,1条着丝粒带和5条中间带。 展开更多
关键词 丹参 染色体 核型 C—banding
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A Quality Assurance Procedure and Evaluation of Rainfall Estimates for C-Band Polarimetric Radar 被引量:9
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作者 HU Zhiqun LIU Liping WANG Lirong 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2012年第1期144-156,共13页
A mobile C-band dual polarimetric weather radar J type (PCDJ), which adopts simultaneous transmission and simultaneous reception (STSR) of horizontally and vertically polarized signals, was first developed in Chin... A mobile C-band dual polarimetric weather radar J type (PCDJ), which adopts simultaneous transmission and simultaneous reception (STSR) of horizontally and vertically polarized signals, was first developed in China in 2008. It was deployed in the radar observation plan in the South China Heavy Rainfall Experiment (SCHeREX) in the summer of 2008 and 2009, as well as in Tropical Western Pacific Ocean Observation Experiments and Research on the Predictability of High Impact Weather Events from 2008 to 2010 in China (TWPOR). Using the observation data collected in these experiments, the radar systematic error and its sources were analyzed in depth. Meanwhile an algorithm that can smooth differential propagation phase (~Dp) for estimating the high-resolution specific differential phase (KDP) was developed. After attenuation correction of reflectivity in horizontal polarization (ZH) and differential reflectivity (ZDR) of PCDJ radar by means of KDP, the data quality was improved significantly. Using quality-controlled radar data, quantitative rainfall estimation was performed, and the resutls were compared with rain-gauge measurements. A synthetic ZH /KDp-based method was analyzed. The results the traditional ZH-based method when the rain suggest that the synthetic method has the advantage over rate is 〉5 mm h^-1. The more intensive the rain rates, the higher accuracy of the estimation. 展开更多
关键词 quality assurance rainfall estimates c-band polarimetric radar
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Physical design and cooling test of C-band standing wave accelerating tube 被引量:4
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作者 柏伟 许州 +1 位作者 金晓 黎明 《Chinese Physics B》 SCIE EI CAS CSCD 2006年第2期319-323,共5页
The physical design and cooling test of a C-band 2MeV standing wave (SW) accelerating tube are described in this paper. The designed accelerating structure consists of 3-cell buncher and 4-cell accelerating section ... The physical design and cooling test of a C-band 2MeV standing wave (SW) accelerating tube are described in this paper. The designed accelerating structure consists of 3-cell buncher and 4-cell accelerating section with a total length of about 163mm, excited with 1MW magnetron. Dynamic simulation presents that about 150mA beam pulse current and 30% capture efficiency can be achieved. By means of nonlinear Gauss fit on electron transverse distribution, the diameter of beam spot FWHM (full width at half maximum of density distribution) is about 0.55mm. Cooling test results of the accelerating tube show that frequencies of cavities are tuned to 5527MHz and the field distribution of bunching section is about 3:9:10. 展开更多
关键词 c-band standing wave accelerating tube space charge effect
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Raindrop Size Distribution Parameters Retrieved from Xinfeng C-Band Polarimetric Radar Observations 被引量:4
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作者 DING Yan WAN Qi-lin +3 位作者 YANG Ling LIU Xian-tong XIA Feng FENG Lu 《Journal of Tropical Meteorology》 SCIE 2020年第3期275-285,共11页
The objective of this research was to acquire a raindrop size distribution(DSDs)retrieved from C-band polarimetric radar observations scheme for the first time in south China.An observation period of the precipitation... The objective of this research was to acquire a raindrop size distribution(DSDs)retrieved from C-band polarimetric radar observations scheme for the first time in south China.An observation period of the precipitation process was selected,and the shape-slope(μ-Λ)relationship of this region was statistically analyzed using the raindrop sample observations from the two-dimensional video disdrometer(2DVD)at Xinfeng Station,Guangdong Province.Simulated data of the C-band polarimetric radar reflectivity ZHHand differential reflectivity ZDRwere obtained through scattering simulation.The simulation data were combined with DSD fitting to determine the ZDR-Λand log10(ZHH/N0)-Λrelationships.Using Xinfeng C-band polarimetric radar observations ZDRand ZHH,the raindrop Gamma size distribution parametersμ,Λ,and N0were retrieved.A scheme for using C-band polarimetric radar to retrieve the DSDs was developed.This research revealed that during precipitation process,the DSDs obtained using the C-band polarimetric radar retrieval scheme are similar to the 2DVD observations,the precipitation characteristics of rainfall intensity(R),mass-weighted mean diameter(Dm)and intercept parameter(Nw)with time obtained by radar retrieval are basically consistent with the observational results of the 2DVD.This scheme establishes the relationship between the observations of the C-band polarimetric radar and the physical quantities of the numerical model.This method not only can test the prediction of the model data assimilation system on the convective scale and determine error sources,but also can improve the microphysical precipitation processes analysis and radar quantitative precipitation estimation.The present research will facilitate radar data assimilation in the future. 展开更多
关键词 c-band polarimetric radar 2DVD raindrop size distribution DSDs retrieval precipitation characteristics
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High Gain UWB Four Elements Antenna Array for C-Band and X-Band Application 被引量:1
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作者 Tamer Gaber Abouelnaga Mazhar Basyouni Tayel Asmaa Fereg Desouky 《Open Journal of Antennas and Propagation》 2020年第2期19-29,共11页
The C-band is allocated for commercial telecommunications via satellites. Amateur satellite operations in the frequency range 5.830 to 5.850 GHz for down-links and 5.650 to 5.670 GHz for up-links are allowed by Intern... The C-band is allocated for commercial telecommunications via satellites. Amateur satellite operations in the frequency range 5.830 to 5.850 GHz for down-links and 5.650 to 5.670 GHz for up-links are allowed by International Telecommunication Union. The X-band is used for terrestrial broadband communication, radar applications, and portions of the X-band are assigned for deep space telecommunications. In this paper, a design of 4 × 1 Ultra Wide Band (UWB) antenna array for C-band and X-band applications is introduced. Metamaterial sixteen-unit cells are incorporated into each antenna element for radiation characteristics enhancement purposes. Permeability and permittivity of metamaterial unit cells are obtained all over the operating bandwidth. UWB unequal power divider is used to feed the proposed four elements antenna array based on Chebyshev excitation method. The proposed antenna has a suitable 3 dB beam width and gains all over the operating bandwidth which extends from 5.6 GHz to 10.9 GHz. The proposed antenna covers 60% and 72.5% of the C-band and X-band, respectively. The proposed antenna is fabricated, measured, and good agreement is obtained between simulated and measured results. The obtained performance ensures the suitability of the proposed antenna array for C-band and X-band applications. 展开更多
关键词 c-band X-BAND METAMATERIAL Antenna Array UWB
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5G发信干扰卫星下行(C-Band)研究
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作者 苏炯 《数字通信世界》 2019年第5期1-2,6,共3页
本文主要介绍了5G基站部署后(以电信3.4-3.5 GHz为例)对附近的卫星地球站下行接收(C-Band,3.625-4.2 GHz)干扰的现象、原理及解决手段,对将来5G业务和卫星业务(C-Band)和平共存提出一些建议。
关键词 5G 卫星下行 c-band 阻塞干扰 带通滤波器
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Design of MEMS C-Band Microstrip Antenna Array Based on High-Resistance Silicon for Intelligent Ammunition
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作者 Xue Zhao Xiao-Ming Liu 《Journal of Electronic Science and Technology》 CAS 2013年第1期101-105,共5页
In recent years, microstrip antennas have been more widely applied in satellite communications, mobile phones, unmanned aerial vehicle (UAV), and weapons. A micro-electro-mechanical systems-based (MEMS-based) high... In recent years, microstrip antennas have been more widely applied in satellite communications, mobile phones, unmanned aerial vehicle (UAV), and weapons. A micro-electro-mechanical systems-based (MEMS-based) high-resistance silicon C-band microstrip antenna array has been designed for the intelligent ammunition. The center frequency is 4.5 GHz. A cavity has been designed in substrate to reduce the dielectric constant of silicon and high-resistance silicon has been used as the material of substrate to improve the gain of antenna. It is very easy to be manufactured by using MEMS technology because of the improved structure of the antenna. The results show that the gain of the antenna is 8 dB and voltage standing wave ratio (VSWR) is less than 2 by the analysis and simulation in high freauencv structure simulator (HFSS). 展开更多
关键词 c-band high frequency structure simulator high-resistance silicon intelligent ammunition microstrip antenna array.
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An Antenna Like Form “Hand” of the Man on Five Bands Wlan/Wimax/Hiper LAN2/C-Band Applications
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作者 Moufida Bouslama Moubarek Traii +1 位作者 Ali Gharsallah Tayeb A. Denidni 《Journal of Electromagnetic Analysis and Applications》 2014年第13期391-396,共6页
In this paper, a new form of printed antenna for a five-band C-band applications is presented. The proposed antenna consists of six simple rectangles, whose geometry resembles all the “hand” of man. By varying the g... In this paper, a new form of printed antenna for a five-band C-band applications is presented. The proposed antenna consists of six simple rectangles, whose geometry resembles all the “hand” of man. By varying the geometries and dimensions of these six rectangles, five different resonance modes can be effectively created for five distinct frequency bands, respectively. The overall dimension of the proposed antenna can reach 45 × 50 × 2.54 mm3. The simulated results show that the presented antenna is adapted to 4.6/5.24/5.6/6.7/7.3 GHz five bands which are applied to C-band. 展开更多
关键词 Patch ANTENNA A Five-Band WIMAX WLAN HIPER LAN2 c-band
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Exploitation of Electromagnetic Models for Sea Wind Speed Estimation from C-Band Sentinel-1 Images
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作者 Tran Vu La Ali Khenchaf +2 位作者 Fabrice Comblet Carole Nahum Helmi Ghanmi 《Journal of Electromagnetic Analysis and Applications》 2016年第3期42-55,共14页
Among the different available wind sources, i.e. in situ measurements, numeric weather models, the retrieval of wind speed from Synthetic Aperture Radar (SAR) data is one of the most widely used methods, since it can ... Among the different available wind sources, i.e. in situ measurements, numeric weather models, the retrieval of wind speed from Synthetic Aperture Radar (SAR) data is one of the most widely used methods, since it can give high wind resolution cells. For this purpose, one can find two principal approaches: via electromagnetic (EM) models and empirical (EP) models. In both approaches, the Geophysical Model Functions (GMFs) are used to describe the relation of radar scattering, wind speed, and the geometry of observations. By knowing radar scattering and geometric parameters, it is possible to invert the GMFs to retrieve wind speed. It is very interesting to compare wind speed estimated by the EM models, general descriptions of radar scattering from sea surface, to the one estimated by the EP models, specific descriptions for the inverse problem. Based on the comparisons, some ideas are proposed to improve the performance of the EM models for wind speed retrieval. 展开更多
关键词 c-band Synthetic Aperture Radar (SAR) Electromagnetic (EM) Scattering Models Radar Cross-Section (RCS) Remote Sensing Small Perturbation Model (SPM) Sea Surface Wind
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Design and study of a C-band pulse compressor for the SXFEL linac 被引量:2
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作者 王超鹏 方文程 +2 位作者 童德春 顾强 赵振堂 《Nuclear Science and Techniques》 SCIE CAS CSCD 2014年第2期1-6,共6页
A C-band RF pulse compressor is in development at SINAP It comprises of two resonant cavities,two mode convertors and a 3 dB power divider.TE_(0.1.15)mode is selected for obtaining higher quality factor Q_0 of the RF ... A C-band RF pulse compressor is in development at SINAP It comprises of two resonant cavities,two mode convertors and a 3 dB power divider.TE_(0.1.15)mode is selected for obtaining higher quality factor Q_0 of the RF pulse compressor cavities,so that the power gain factor can be 3.2,which is supposed to multiply the RF power from 50 MW to 1 60 MW.In this paper,we report our work on C-band RF pulse compressor,namely the design simulation and cold test results. 展开更多
关键词 脉冲压缩器 C波段 直线加速器 设计 功率分配器 RF功率 模式转换器 高品质因数
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Leaf vein micronetwork engineering enhanced energy conversion strategy for C-band ultralight yet tunable microwave absorption
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作者 Chunyan Ding Chengshuai Shao +11 位作者 Zhen Wang Zhuoyang Li Xue Guo Xiaozhen Ren Hongchang Pei Songsong Wu Qianqian Zhang Chuncheng Wei Long Xia Bo Zhong Guangwu Wen Xiaoxiao Huang 《Rare Metals》 2025年第9期6513-6530,共18页
Lightweight materials with wide absorption capabilities,particularly in the C-band,have remained a challenge thus far.Recent research has indicated that effective absorption networks built by microfiber polarization l... Lightweight materials with wide absorption capabilities,particularly in the C-band,have remained a challenge thus far.Recent research has indicated that effective absorption networks built by microfiber polarization loss can be a significant factor in increasing the effective absorption bandwidth(EAB).In this study,leaf vein-like carbon(LVC)was synthesized using an in situ blowing strategy.Taking inspiration from photosynthesis energy conversion mechanisms,a leaf veins-like hierarchical structure was created to establish an effective impedance-matching network and generate a high-density polarization region through leaf vein microfibers.This enhanced polarization relaxation effectively broadens the EAB of the LVC.At a low filling ratio of 6.3 wt%,the EAB of the LVC covers 80%of the C-band,as well as100%of the X-band and Ku-band.Achieving such a wide EAB in the C-band,especially in the multi-band context,relies on impedance matching and optimized polarization relaxation.This work demonstrates the crucial role of leaf vein micronetwork engineering in enhancing the C-band absorption properties of carbon-based materials,thus providing a viable reference for the development of lightweight,broadband,and highly absorptive materials for electromagnetic applications. 展开更多
关键词 Microwave absorption Impedance matching Ultralight Microfiber c-band
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Interference of 5G with aircraft radio altimeters: How to protect aviation safety in rapidly changing environment of expanding C-band networks
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作者 L.Kurzweil 《Aerospace Traffic and Safety》 2024年第2期155-164,共10页
The topic of 5G network development is resonating in the aviation world.According to the information from aircraft manufacturers,aircraft radio altimeters could be interfered with in the C-band frequency spectrum.Radi... The topic of 5G network development is resonating in the aviation world.According to the information from aircraft manufacturers,aircraft radio altimeters could be interfered with in the C-band frequency spectrum.Radio altimeters are electronic devices that measure the precise altitude above the terrain and are very important for flight safety in the phase of the final approach and landing.The problem statement was,firstly,how safe the aircraft approaching and departing Prague Airport have been and secondly,how to protect aviation safety in the rapidly changing technological environment of expanding C-band networks.The methodology of the paper was,firstly,to assess the impact of existing installations on flight operations using the Safety Management System principles;and secondly,to assess the impact of planned C-band installations on flight operations according to the technical parameters of proposed installations.This is a methodology proposed in the paper for newly defined coordination zones around Prague Airport.The methodology can be used by other airports aiming to assess the risks of C-band interference.Through all safety promotion means,the crews were encouraged and motivated to report all outages of radio altimeters via safety reporting channels.From the period September 2021 to November 2023,one safety report concerning the radio altimeter was received.The report informed about a safety incident,the failure of the radio altimeter.An investigation into the causes of the incident has taken place and the results are provided and discussed in the paper.According to the data collected and investigated,the failures of radio altimeters are rare and no case has been discovered where the failure of the radio altimeter of an aircraft on the ground or an aircraft arriving or departing Prague RuzyněAirport was caused by interference with C-band.The new C-band installations can be assessed according to the previously published model of the French civil aviation authority,however,a modification of the evaluation procedure is proposed in the paper. 展开更多
关键词 Safety management system Safety of flight Mobile communication 5th generation network c-band frequencies Radio altimeter Interference Aircraft final approach
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国产化C波段加速器性能参数实验研究 被引量:1
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作者 柏伟 马国武 +3 位作者 张鲁奇 张德敏 宋志大 孙迪敏 《强激光与粒子束》 北大核心 2025年第4期73-77,共5页
为了在国产化配套条件下,开展C波段小型化加速器整机技术研究,中国工程物理研究院应用电子学研究所在研制出了多种C波段小型化驻波加速管的基础上,建立了以国产零部件(磁控管、环形器、高压电源等)为主要构件的C波段加速器X射线源整机系... 为了在国产化配套条件下,开展C波段小型化加速器整机技术研究,中国工程物理研究院应用电子学研究所在研制出了多种C波段小型化驻波加速管的基础上,建立了以国产零部件(磁控管、环形器、高压电源等)为主要构件的C波段加速器X射线源整机系统,并进一步利用该加速器X射线源整机系统作为测试平台,开展了大功率热测实验工作。在热测实验中,按照国标《GB/T 20129-2015无损检测用电子直线加速器》规定的测试原理和方法,测试了加速器的主要性能指标:利用钢衰减法测试了加速器的能量、利用“三明治”测试了加速器的焦点尺寸等。最终的测试结果表明,加速器焦点尺寸约为1.2 mm,加速器的能量在3~4 MeV范围可实现连续可调,20 min内加速器的剂量率波动小于±3%。该研究结果表明,国内C波段小型化加速器整机配套环境和整机性能已经基本上可以满足当前部分能量段的小型化加速器整机系统的研制和使用需求。 展开更多
关键词 C波段 加速器 X射线源 国产化 小型化
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南方先进光源C波段光阴极电子枪微波设计
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作者 刘盛进 姜世民 +4 位作者 刘星光 肖永川 曹秀霞 吕永佳 李晓 《强激光与粒子束》 北大核心 2025年第1期162-168,共7页
作为南方先进光源直线段注入器重要设备,开展了C波段光阴极电子枪研究,包括驻波腔微波设计和耦合器设计。其中驻波腔采用3.6腔结构,π模加速模式,工作频率为5.712 GHz;耦合器采用同轴耦合方式。利用Superfish及CST完成了腔体微波结构设... 作为南方先进光源直线段注入器重要设备,开展了C波段光阴极电子枪研究,包括驻波腔微波设计和耦合器设计。其中驻波腔采用3.6腔结构,π模加速模式,工作频率为5.712 GHz;耦合器采用同轴耦合方式。利用Superfish及CST完成了腔体微波结构设计,优化盘片的形状,降低腔体表面最大电场,从而有利于提高腔体加速场强;利用COMSOL开展了腔体水冷系统的分析,优化设计水路,减少腔体由于功率负载所造成的频率偏移,控制腔体温度的上升,保持腔体最大温升小于20℃。在18.15 MW的入腔功率下,阴极面最高场强为180 MV/m,腔体表面最大场强与阴极面场强比值约为0.934 6,腔体Q值大于10 000。通过对耦合器的设计,抑制二极模和四极模的传输,S_(11)参数小于-40 dB。 展开更多
关键词 南方先进光源 光阴极电子枪 微波结构 耦合器 C波段
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C波段9 MeV小焦点驻波电子直线加速结构设计 被引量:1
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作者 李诗琳 杨京鹤 +3 位作者 范雨轩 王博 杨誉 雷瀚 《原子能科学技术》 北大核心 2025年第1期213-219,共7页
高能X射线检测是重要的无损检测手段之一,X射线源的焦点尺寸对检测精度有着重要影响。为了满足高分辨率无损检测对高性能加速器X射线源的需求,本文设计了一个C波段小焦点驻波电子直线加速结构。基于静电聚焦和射频相位聚焦原理,对加速... 高能X射线检测是重要的无损检测手段之一,X射线源的焦点尺寸对检测精度有着重要影响。为了满足高分辨率无损检测对高性能加速器X射线源的需求,本文设计了一个C波段小焦点驻波电子直线加速结构。基于静电聚焦和射频相位聚焦原理,对加速结构进行了物理设计,使用Superfish和Parmela对加速结构进行射频设计和束流动力学计算,使用CST电磁场模拟优化腔链结构,并对束流动力学设计结果进行验证。加速结构共包括3个聚束腔单元和19个光速腔单元,总长度约为548 mm,加速结构的束流能量达到9.11 MeV,焦点尺寸为0.45 mm,俘获效率为35.8%。 展开更多
关键词 小焦点 C波段 驻波加速结构
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相控阵测控技术(六):相控阵的时变滤波特性及其对通信性能的影响 被引量:1
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作者 刘嘉兴 《电讯技术》 北大核心 2025年第5期727-733,共7页
孔径渡越效应是相控阵的一个特殊问题,针对此推导出了它产生的幅度/相位频率特性表达式,提出将相控阵等效为传输链路中的一个时变滤波器,构成相控阵带限通信系统,进而可利用通信系统的一些成熟理论对其有效性、可靠性、适应性等主要性... 孔径渡越效应是相控阵的一个特殊问题,针对此推导出了它产生的幅度/相位频率特性表达式,提出将相控阵等效为传输链路中的一个时变滤波器,构成相控阵带限通信系统,进而可利用通信系统的一些成熟理论对其有效性、可靠性、适应性等主要性能指标进行深入研究。作为例子,分析了它引起的附加误码及其等效的载噪比恶化等通信可靠性指标,并给出了改进措施。 展开更多
关键词 相控阵测控系统 孔径渡越效应 时变滤波 限带通信 最佳传输 匹配滤波 奈奎斯特滤波
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钝吻黄盖鲽染色体核型及带型特征
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作者 赵梓涵 崔爱君 +4 位作者 张宁 柳学周 徐永江 赵日祥 孙宗哲 《水产科学》 北大核心 2025年第5期727-736,共10页
钝吻黄盖鲽是我国重要的土著海水经济鱼类,目前其遗传特性尚未完全明了。为深入探究钝吻黄盖鲽染色体核型及带型特征,选取全长3~5cm的3~4月龄钝吻黄盖鲽50尾为试验材料,采用秋水仙素浸泡背鳍,染色体标本制备包括低渗处理、卡诺氏固定液... 钝吻黄盖鲽是我国重要的土著海水经济鱼类,目前其遗传特性尚未完全明了。为深入探究钝吻黄盖鲽染色体核型及带型特征,选取全长3~5cm的3~4月龄钝吻黄盖鲽50尾为试验材料,采用秋水仙素浸泡背鳍,染色体标本制备包括低渗处理、卡诺氏固定液固定及空气干燥法等,对钝吻黄盖鲽染色体中期分裂相及显带特征(C-带、Ag-NORs带和G-带)进行研究。试验结果显示:钝吻黄盖鲽具有典型的端部着丝粒染色体,核型公式为2n=48t,臂数NF=48,未观察到多倍体、异型性染色体及随体结构。C-带中第17对染色体呈整条阳性深染,第9对染色体则呈整条阴性浅染,前3对染色体具有端部C-带,其余染色体仅显示着丝粒C-带,异染色质总量为32.05%;Ag-NORs带定位于第2对染色体长臂末端;G-带分析共检测到58条深染带和38条浅染带,第11对染色体完全浅染。试验结果可为钝吻黄盖鲽染色体组学、物种鉴定及遗传育种等提供参考依据。 展开更多
关键词 钝吻黄盖鲽 染色体 核型分析 C-带 AG-NORS带 G-带
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云纹石斑鱼(♀)×云龙石斑鱼(♂)回交子代染色体核型及带型分析
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作者 王世豪 李振通 +7 位作者 张春白 丁棠棠 杨丰帆 刘阳 黎琳琳 田永胜 李胜忠 贺新磊 《海洋与湖沼》 北大核心 2025年第5期1300-1310,共11页
为了分析云龙石斑鱼回交F_(2)代的染色体核型及带型特征,揭示其遗传变异规律和染色体结构特性,系统解析染色体数目、核型组成、异染色质分布及核仁组织区数量,以云纹石斑鱼(Epinephelus moara)为母本、云龙石斑鱼(E.moara♀×E.lanc... 为了分析云龙石斑鱼回交F_(2)代的染色体核型及带型特征,揭示其遗传变异规律和染色体结构特性,系统解析染色体数目、核型组成、异染色质分布及核仁组织区数量,以云纹石斑鱼(Epinephelus moara)为母本、云龙石斑鱼(E.moara♀×E.lanceolatus♂)为父本进行回交,获得正常发育的回交子代MLBCF_(2)。以回交子代MLBCF_(2)为材料,采用植物血球凝集素(PHA)和秋水仙素体腹腔注射法,取头肾细胞制备染色体标本,对其染色体核型、C-带、G-带和Ag-NORs进行分析。回交F_(2)代染色体2n=48,核型组成为4sm+44t,NF=52。染色体均呈现C-带阳性,可分为4种类型:着丝粒C-带、端粒C-带、居间C-带和整体C-带。在第1对染色体显示深染G-带的部分最多,深染带数量在3~9条之间,未发现整体深染G-带,其带纹在每对染色体上的数目及分布具有明显特征性。间期细胞的AgNORs数目在不同的细胞中表现出多态性,数目为1~3个,出现1个Ag-NORs位点的频率为39.6%,出现2个位点的频率为56.6%,出现3个位点的频率为3.8%;中期细胞的Ag-NORs位点则主要出现在第24对同源染色体上,未发现有Ag-NORs联合的现象。回交F_(2)代染色体结构保守性与变异性并存,第24对染色体的异染色质特征为遗传标记提供了依据。研究结果为石斑鱼杂交育种、种质改良及染色体进化研究提供了重要的细胞遗传学资料。 展开更多
关键词 云龙石斑鱼回交F_(2)代 染色体 C-带 G-带 AG-NORS 带型
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