Substantial evidence points to the early onset of peripheral inflammation in the development of Parkinson's disease(PD),supporting the“body-first”hypothesis.However,there remains a notable absence of PD-specific...Substantial evidence points to the early onset of peripheral inflammation in the development of Parkinson's disease(PD),supporting the“body-first”hypothesis.However,there remains a notable absence of PD-specific animal models induced by inflammatory cytokines.This study introduces a novel mouse model of PD driven by the proinflammatory cytokine CXCL1,identified in our previous research.The involvement of CXCL1 in PD pathogenesis was validated using subacute and chronic MPTP-induced mouse models.Based on these findings,2-month-old C57BL/6J mice were intravenously administered CXCL1(20 ng/kg/day)for 2 weeks(5 days per week),successfully replicating motor deficits and pathological alterations in the substantia nigra observed in the chronic MPTP model.These results demonstrate the potential of CXCL1-induced inflammation as a mechanism for PD modeling.The model revealed activation of the PPAR signaling pathway in CXCL1-mediated neuronal damage by CXCL1.Linoleic acid,a PPAR-γactivator,significantly mitigated MPTPand CXCL1-induced toxicity and reduced serum CXCL1levels.In addition,the CXCL1-injected mouse model shortened the timeline for developing chronic PD mouse model to 2 weeks,offering an efficient platform for studying inflammation-driven processes in PD.The findings provide critical insights into the inflammatory mechanisms underlying PD and identify promising therapeutic targets for intervention.展开更多
Alzheimer'sdisease(AD)isaprogressive neurodegenerative disorder characterized by cognitive impairment and distinct neuropathological features,including amyloid-βplaques,neurofibrillary tangles,and reactive astrog...Alzheimer'sdisease(AD)isaprogressive neurodegenerative disorder characterized by cognitive impairment and distinct neuropathological features,including amyloid-βplaques,neurofibrillary tangles,and reactive astrogliosis.Developing effective diagnostic,preventative,and therapeutic strategies for AD necessitates the establishment of animal models that accurately recapitulate the pathophysiological processes of the disease.Existing transgenic mouse models have significantly contributed to understanding AD pathology but often fail to replicate the complexity of human AD.Additionally,these models are limited in their ability to elucidate the interplay among amyloid-βplaques,neurofibrillary tangles,and reactive astrogliosis due to the absence of spatially and temporally specific genetic manipulation.In this study,we introduce a novel AD mouse model(APP/PS1-TauP301L-Adeno mice)designed to rapidly induce pathological symptoms and enhance understanding of AD mechanisms.Neurofibrillary tangles and severe reactive astrogliosis were induced by injecting AAVDJ-EF1a-hTauP301L-EGFP and Adeno-GFAP-GFP viruses into the hippocampi of 5-month-old APP/PS1 mice.Three months post-injection,these mice exhibited pronounced astrogliosis,substantial amyloid-βplaque accumulation,extensiveneurofibrillarytangles,accelerated neuronal loss,elevated astrocytic GABA levels,and significant spatial memory deficits.Notably,these pathological features were less severe in AAVTauP301L-expressing APP/PS1 mice without augmented reactive astrogliosis.These findings indicate an exacerbating role of severe reactive astrogliosis in amyloid-βplaque and neurofibrillary tangle-associated pathology.The APP/PS1-TauP301L-Adeno mouse model provides a valuable tool for advancing therapeutic research aimed at mitigating the progression of AD.展开更多
Based on the hindcasts from five subseasonal-to-seasonal(S2S)models participating in the S2S Prediction Project,this study evaluates the performance of the multimodel ensemble(MME)approach in predicting the subseasona...Based on the hindcasts from five subseasonal-to-seasonal(S2S)models participating in the S2S Prediction Project,this study evaluates the performance of the multimodel ensemble(MME)approach in predicting the subseasonal precipitation anomalies during summer in China and reveals the contributions of possible driving factors.The results suggest that while single-model ensembles(SMEs)exhibit constrained predictive skills within a limited forecast lead time of three pentads,the MME illustrates an enhanced predictive skill at a lead time of up to four pentads,and even six pentads,in southern China.Based on both deterministic and probabilistic verification metrics,the MME consistently outperforms SMEs,with a more evident advantage observed in probabilistic forecasting.The superior performance of the MME is primarily attributable to the increase in ensemble size,and the enhanced model diversity is also a contributing factor.The reliability of probabilistic skill is largely improved due to the increase in ensemble members,while the resolution term does not exhibit consistent improvement.Furthermore,the Madden–Julian Oscillation(MJO)is revealed as the primary driving factor for the successful prediction of summer precipitation in China using the MME.The improvement by the MME is not solely attributable to the enhancement in the inherent predictive capacity of the MJO itself,but derives from its capability in capturing the more realistic relationship between the MJO and subseasonal precipitation anomalies in China.This study establishes a scientific foundation for acknowledging the advantageous predictive capability of the MME approach in subseasonal predictions of summer precipitation in China,and sheds light on further improving S2S predictions.展开更多
BACKGROUND At present,there is a lack of non-invasive indicators to evaluate the changes in endoscopic activity between two visits for patients with Crohn's disease(CD).AIM To develop a model for predicting whethe...BACKGROUND At present,there is a lack of non-invasive indicators to evaluate the changes in endoscopic activity between two visits for patients with Crohn's disease(CD).AIM To develop a model for predicting whether endoscopic activity will improve in CD patients.METHODS This is a single-center retrospective study that included patients diagnosed with CD from January 2014 to December 2022.The patients were randomly divided into a modeling group(70%)and an internal validation group(30%),with an external validation group from January 2023 to March 2024.Univariate and binary logistic regression analyses were conducted to identify independent risk factors,which were used to construct a nomogram model.The model's performance was evaluated using receiver operating characteristic curves,calibration curves,and decision curve analysis(DCA).Additionally,further sensitivity analyses were performed.RESULTS One hundred seventy patients were included in the training group,while 64 were included in the external validation group.A binary logistic stepwise regression analysis revealed that the changes in the amplitudes of albumin(ALB)and fibrinogen(FIB)were independent risk factors for endoscopic improvement.A nomogram model was developed based on these risk factors.The area under the curve of the model for the training group,internal validation group,and external validation group were 0.802,0.788,and 0.787,respectively.The average absolute errors of the calibration curves were 0.011,0.016,and 0.018,respectively.DCA indicated that the model performs well in clinical practice.Additionally,sensitivity analysis demonstrated that the model has strong robustness and applicability.CONCLUSION Our study shows that changes in the amplitudes of ALB and FIB are effective predictors of endoscopic improvement in patients with CD during follow-up visits compared to their previous ones.展开更多
Bacckground:Based on the 3C model,this study explores the current situation of HPV vaccine hesitancy among women of childbearing age and the factors influencing vaccine hesitancy.Methods:Based on the free cervical can...Bacckground:Based on the 3C model,this study explores the current situation of HPV vaccine hesitancy among women of childbearing age and the factors influencing vaccine hesitancy.Methods:Based on the free cervical cancer screening project in Baoan District,this study designed a questionnaire under the framework of vaccine hesitation 3C theory and carried out a self-filling electronic questionnaire survey among women of childbearing age.Results:The rate of HPV vaccination awareness among women of childbearing age in Bao’an District was 93.25%.HPV vaccine acceptance reached 71.55%,and 24.59%of the survey respondents experienced HPV vaccine hesitation,a high percentage of whom were hesitant.The influencing factors of HPV vaccine hesitation among women of childbearing age were perceived necessity of HPV vaccination(0.482),no one around them receiving the HPV vaccine(0.411),perception of the price of the vaccine(0.354),degree of trust in the safety of the vaccine(0.223),and degree of concern about the HPV vaccine(0.153).Conclusion:The 3C model can be used for the study of HPV vaccine hesitancy.Strengthening the publicity of HPV vaccination and improving women’s knowledge of the HPV vaccine can reduce their hesitation toward HPV vaccination.展开更多
To promote the global dissemination of Chinese culture,the translation of Chinese classic literature has garnered widespread attention in the translation field.Literary translation criticism plays an essential role in...To promote the global dissemination of Chinese culture,the translation of Chinese classic literature has garnered widespread attention in the translation field.Literary translation criticism plays an essential role in the development of translation endeavors.This paper will analyze three versions of English translations of Shen Congwen’s novella Border Town using Reiss’s translation criticism model and summarize their performance in terms of formal equivalence and aesthetic effect,linguistic components,and extra-linguistic components.展开更多
Based on BERTopic Model,the paper combines qualitative and quantitative methods to explore the reception of Can Xue’s translated works by analyzing readers’book reviews posted on Goodreads and Lovereading.We first c...Based on BERTopic Model,the paper combines qualitative and quantitative methods to explore the reception of Can Xue’s translated works by analyzing readers’book reviews posted on Goodreads and Lovereading.We first collected book reviews from these two well-known websites by Python.Through topic analysis of these reviews,we identified recurring topics,including details of her translated works and appreciation of their translation quality.Then,employing sentiment and content analysis methods,the paper explored the emotional attitudes and the specific thoughts of readers toward Can Xue and her translated works.The fingdings revealed that,among the 408 reviews,though the reception of Can Xue’s translated works was relatively positive,the current level of attention and recognition remains insufficient.However,based on the research results,the paper can derive valuable insights into the translation and dissemination processes such as adjusting translation and dissemination strategies,so that the global reach of Chinese literature and culture can be better facilitated.展开更多
Peyronie’s disease(PD)is a disorder characterized by fibrous plaque formation in the penile tissue that leads to curvature and complications in advanced stages.In this study,we aimed to compare four injectable induct...Peyronie’s disease(PD)is a disorder characterized by fibrous plaque formation in the penile tissue that leads to curvature and complications in advanced stages.In this study,we aimed to compare four injectable induction agents for the establishment of a robust rat model of PD:transforming growth factor-β1(TGF-β1),fibrin,sodium tetradecyl sulfate(STS)combined with TGF-β1,and polidocanol(POL)combined with TGF-β1.The results showed that injection of TGF-β1 or fibrin into the tunica albuginea induced pathological endpoints without causing penile curvature.The STS+TGF-β1 combination resulted in both histological and morphological alterations,but with a high incidence of localized necrosis that led to animal death.The POL+TGF-β1 combination produced pathological changes and curvature comparable to STS+TGF-β1 and led to fewer complications.In conclusion,fibrin,STS+TGF-β1,and POL+TGF-β1 all induced PD with a certain degree of penile curvature and histological fibrosis in rats.The POL+TGF-β1 combination offered comparatively greater safety and clinical relevance and may have the greatest potential for PD research using model rats.展开更多
This paper,grounded in the theory of business model innovation,examines the Chinese People’s Health Press(人民卫生出版社)as a case study to explore strategies for innovating business models within the context of prof...This paper,grounded in the theory of business model innovation,examines the Chinese People’s Health Press(人民卫生出版社)as a case study to explore strategies for innovating business models within the context of professional publishing convergence development.The research posits that effective business model innovation in this domain necessitates a comprehensive reform of its constituent elements.It advocates for a systematic approach to reconstructing value propositions,enhancing value creation and delivery processes,and optimizing value capture mechanisms to achieve desired outcomes.展开更多
With the widespread application of large language models(LLMs)in natural language processing and code generation,traditional High-Level Language Programming courses are facing unprecedented challenges and opportunitie...With the widespread application of large language models(LLMs)in natural language processing and code generation,traditional High-Level Language Programming courses are facing unprecedented challenges and opportunities.As a core programming language for computer science majors,C language remains irreplaceable due to its foundational nature and engineering adaptability.This paper,based on the rapid development of large model technologies,proposes a systematic reform design for C language teaching,focusing on teaching objectives,content structure,teaching methods,and evaluation systems.The article suggests a teaching framework centered on“human-computer collaborative programming,”integrating prompt training,AI-assisted debugging,and code generation analysis,aiming to enhance students’problem modeling ability,programming expression skills,and AI collaboration literacy.展开更多
High-fidelity tactile rendering offers significant potential for improving the richness and immersion of touchscreen interactions.This study focuses on a quantitative description of tactile rendering fidelity using a ...High-fidelity tactile rendering offers significant potential for improving the richness and immersion of touchscreen interactions.This study focuses on a quantitative description of tactile rendering fidelity using a custom-designed hybrid electrovibration and mechanical vibration(HEM)device.An electrovibration and mechanical vibration(EMV)algorithm that renders 3D gratings with different physical heights was proposed and shown to achieve 81%accuracy in shape recognition.Models of tactile rendering fidelity were established based on the evaluation of the height discrimination threshold,and the psychophysical-physical relationships between the discrimination and reference heights were well described by a modification of Weber’s law,with correlation coefficients higher than 0.9.The physiological-physical relationship between the pulse firing rate and the physical stimulation voltage was modeled using the Izhikevich spiking model with a logarithmic relationship.展开更多
The numerical simulation of the fluid flow and the flexible rod(s)interaction is more complicated and has lower efficiency due to the high computational cost.In this paper,a semi-resolved model coupling the computatio...The numerical simulation of the fluid flow and the flexible rod(s)interaction is more complicated and has lower efficiency due to the high computational cost.In this paper,a semi-resolved model coupling the computational fluid dynamics and the flexible rod dynamics is proposed using a two-way domain expansion method.The gov-erning equations of the flexible rod dynamics are discretized and solved by the finite element method,and the fluid flow is simulated by the finite volume method.The interaction between fluids and solid rods is modeled by introducing body force terms into the momentum equations.Referred to the traditional semi-resolved numerical model,an anisotropic Gaussian kernel function method is proposed to specify the interactive forces between flu-ids and solid bodies for non-circle rod cross-sections.A benchmark of the flow passing around a single flexible plate with a rectangular cross-section is used to validate the algorithm.Focused on the engineering applications,a test case of a finite patch of cylinders is implemented to validate the accuracy and efficiency of the coupled model.展开更多
The work considers the problem of gas hydrate dissociation in a porous medium using the two-term Forchheimer law,corresponding to high flow rates of reservoir fluids.Such rates can arise during the decomposition of ga...The work considers the problem of gas hydrate dissociation in a porous medium using the two-term Forchheimer law,corresponding to high flow rates of reservoir fluids.Such rates can arise during the decomposition of gas hydrates,since a large amount of gas is released.Intensive emissions of gases from the earth’s interior are observed on the ocean floor.They are also associated with a large number of subvertical geological structures under the ocean floor,coming to the surface in the formof local ring funnels(pockmarks).Many similar objects have also been found on land.Particular interest in this problemis caused by climate threats associated with the release of greenhouse gases.The movement of gas released fromthe hydrate to the breakthrough channel is similar to the gas inflow to the well(without hydrate),which is usually described by a two-term filtration law.In this work,a mathematical model of gas hydrate dissociation with a nonlinear Forchheimer-type law ofmotion is developed.The systemis split in two blocks by physical processes,taking into account the quadratic correction to the velocity in the filtration law.The first block is responsible for the convective transfer of saturation parameters in the model,water,gas and hydrate saturations are taken into account.The second block corresponds to the equation of dissipative piezoconductivity with a different number of thermodynamic degrees of freedom,taking into account heat and mass transfer in a porous medium.The performed splitting allows using explicit-implicit difference schemes when solving problems and avoiding strong refinement of the step in time and space.For numerical modeling,the support operator method is used,which makes it possible to discretize partial differential equations on irregular grids,which allows taking into account the complex geometry and lithology of the reservoir.A difference scheme based on the support operator method is developed,which,due to the mutually consistent approximation of vector analysis operations(divergence and gradient),allows to take into account the various flux laws between adjacent grid cells,including quadratic corrections to the velocity.Based on the developed numerical algorithms and their program implementations,calculations of gas hydrate dissociation are performed both in a reservoir of simple geometric structure and in a heterogeneous reservoir of complex configuration.The results obtained correspond to the physics of the processes under consideration.展开更多
Against the backdrop of globalization and regional economic integration,border tourism,as a unique form of tourism,is increasingly emerging as a significant driving force for promoting economic development in border r...Against the backdrop of globalization and regional economic integration,border tourism,as a unique form of tourism,is increasingly emerging as a significant driving force for promoting economic development in border regions,fostering cultural exchange,and strengthening international cooperation.The northwestern Yunnan region,located in China’s Southwestern frontier,is a key area bordering Southeast and South Asian countries,endowed with abundant border tourism resources.This article delves into the border tourism models and pathways for border development and prosperity in ethnic regions,using a case study of the Northwest Yunnan region to analyze the mechanisms and influencing factors of border tourism in promoting local economic development,social progress,and cultural heritage.This provides empirical evidence for further understanding the development patterns of border tourism and also contributes to strengthening ethnic unity and maintaining harmony and stability in border regions.This study addresses the current research gap in exploring the development models and pathways of border tourism in specific regions,offering valuable references and insights for future related research.展开更多
Using the“Civil Aviation Oral English”course as a case study,we operationalize the ideological and political education(IPE)integrated evaluation system for oral English courses in higher vocational education,which i...Using the“Civil Aviation Oral English”course as a case study,we operationalize the ideological and political education(IPE)integrated evaluation system for oral English courses in higher vocational education,which is based on Kirkpatrick’s Model,into a tripartite teaching evaluation system.The system encompasses three components such as learning objective attainment,individual developmental progress,and value-added competency assessment.The proposed system facilitates three-dimensional,multi-level,and scientifically validated teaching evaluation.This approach aligns with the holistic development of students’knowledge structures,professional competencies,moral qualities,and emotional intelligence.The study provides novel methodological perspectives for IPE evaluation in vocational oral English instruction,particularly in:curriculum-based ideological mapping,competency growth metrics,and value internalization assessment.展开更多
Chikungunya is a mosquito-borne viral infection caused by the chikungunya virus(CHIKV).It is characterized by acute onset of high fever,severe polyarthralgia,myalgia,headache,and maculopapular rash.The virus is rapidl...Chikungunya is a mosquito-borne viral infection caused by the chikungunya virus(CHIKV).It is characterized by acute onset of high fever,severe polyarthralgia,myalgia,headache,and maculopapular rash.The virus is rapidly spreading and may establish in new regions where competent mosquito vectors are present.This research analyzes the regulatory dynamics of a stochastic differential equation(SDE)model describing the transmission of the CHIKV,incorporating seasonal variations,immunization efforts,and environmentalffuctuations modeled through Poisson random measure noise under demographic heterogeneity.The model guarantees the existence of a global positive solution and demonstrates periodic dynamics driven by environmental factors.A key contribution of this study is the formulation of a stochastic threshold parameter,R0L,which characterizes the conditions for disease persistence or extinction under random environmental inffuences.Although our analysis highlights age-speciffc heterogeneities to illustrate differential transmission risks,the framework is general and can incorporate other vulnerable demographic groups,ensuring broader applicability of the results.Using the Monte Carlo Markov Chain(MCMC)method,we estimate R0L=1.4978(95%C-I:1.4968–1.5823)based on CHIKV data from Florida,USA,spanning 2005 to 2017,suggesting that the outbreak remains active and requires targeted control strategies.The effectiveness of immunization,screening,and treatment strategies varies depending on the prioritized demographic groups,due to substantial differences in CHIKV incidence across age categories in the USA.Numerical simulations were conducted using the truncated Euler–Maruyama method to robustly capture the stochastic dynamics of CHIKV transmission with Poissondriven jumps.Employing an iterative approach and assuming mild convexity conditions,we formulated and solved a parameterized near-optimality problem using the Ekeland variational principle.Ourffndings indicate that vaccination campaigns are signiffcantly more effective when focused on vulnerable adults over the age of 66,as well as individuals aged 21 to 25.Furthermore,enhancements in vaccine effcacy,diagnostic screening,and treatment protocols all contribute substantially to minimizing infection rates compared to current standard approaches.These insights support the development of targeted,age-speciffc public health interventions that can signiffcantly improve the management and control of future CHIKV outbreaks.展开更多
Driven by the dual carbon goals and the national strategy for the high-quality development of the advanced manufacturing industry,along with the trend of economic transformation,China’s new energy vehicle market has ...Driven by the dual carbon goals and the national strategy for the high-quality development of the advanced manufacturing industry,along with the trend of economic transformation,China’s new energy vehicle market has experienced explosive growth,which has spurred a surge in the construction of domestic charging infrastructure.Charging infrastructure not only supports achieving dual carbon goals and the development of the new energy vehicle industry but also undertakes the new historical mission of infrastructure in China’s new development stage,becoming a crucial infrastructure connecting energy decarbonization and transportation electrification[1].In recent years,central and local governments have placed great emphasis on this field.They have introduced policies from various angles,including comprehensive macro-level measures,government planning,financial subsidies,charging rates,industry management,and scientific and technological innovation,to promote the moderately advanced development of charging facilities.展开更多
BACKGROUND Syngeneic orthotopic tumor models offer an optimal functional tumor–immune interface for hepatocellular carcinoma research.Yet,unpredictable growth kinetics and spontaneous regression pose major obstacles....BACKGROUND Syngeneic orthotopic tumor models offer an optimal functional tumor–immune interface for hepatocellular carcinoma research.Yet,unpredictable growth kinetics and spontaneous regression pose major obstacles.Efficient induction protocols and continuous monitoring are therefore essential.Routine exploratory surgeries are ethically untenable,making non-invasive imaging modalities attractive alternatives.High-resolution magnetic resonance imaging and microcomputed tomography deliver detailed insights but incur substantial equipment costs,radiation risks,time demands,and require specialized expertise—challenges that limit their routine use.In contrast,ultrasound(US)imaging emerges as a cost-effective,radiation-free,and rapid approach,facilitating practical and ethical longitudinal assessment of tumor progression in preclinical studies.AIM To optimize the orthotopic hepatocellular carcinoma model and evaluate the potential of US imaging for accurate and cost-effective tumor monitoring.METHODS Hepatocellular carcinoma was induced in 28 Sprague Dawley rats by implanting 5×10^(6) N1S1 cells into the left lateral hepatic lobe.Tumor progression was monitored weekly via US.Upon reaching 100-150 mm^(3),an experimental group(n=14)received Sorafenib(40 mg/kg)orally on alternate days for 28 days;efficacy was compared to untreated controls.US accuracy was validated against micro-computed tomography,gross caliper measurements and histopathological analysis.Reliability and operator proficiency in US assessment were also evaluated.RESULTS US images procured 7-day post-surgery revealed a well-defined hypoechoic nodule at the left liver lobe tip,confirming successful tumor induction(mean volume 130±39 mm^(3)).Only three animals exhibited spontaneous regression by week 2,underscoring the model’s stability.Sorafenib treatment elicited a marked tumor reduction(678±103 mm^(3))vs untreated control(6005±1760 mm^(3)).US assessment demonstrated robust intra and interobserver reproducibility with high sensitivity and specificity for tumor detection.Moreover,US derived volumes correlated strongly with gross caliper measurements,histopathological analysis,and microcomputed tomography imaging,validating its reliability as a non-invasive monitoring tool in preclinical hepatocellular carcinoma studies.CONCLUSION The results demonstrate that US imaging is a reliable,cost-effective,and animal sparing approach with an easy tomaster protocol,enabling monitoring of tumor progression and therapeutic response in orthotopic liver tumor models.展开更多
基金supported by the National Natural Science Foundation of China (32471049,32170984,32471188,32200802)Natural Science Foundation of Shandong Province (ZR2023QH110)。
文摘Substantial evidence points to the early onset of peripheral inflammation in the development of Parkinson's disease(PD),supporting the“body-first”hypothesis.However,there remains a notable absence of PD-specific animal models induced by inflammatory cytokines.This study introduces a novel mouse model of PD driven by the proinflammatory cytokine CXCL1,identified in our previous research.The involvement of CXCL1 in PD pathogenesis was validated using subacute and chronic MPTP-induced mouse models.Based on these findings,2-month-old C57BL/6J mice were intravenously administered CXCL1(20 ng/kg/day)for 2 weeks(5 days per week),successfully replicating motor deficits and pathological alterations in the substantia nigra observed in the chronic MPTP model.These results demonstrate the potential of CXCL1-induced inflammation as a mechanism for PD modeling.The model revealed activation of the PPAR signaling pathway in CXCL1-mediated neuronal damage by CXCL1.Linoleic acid,a PPAR-γactivator,significantly mitigated MPTPand CXCL1-induced toxicity and reduced serum CXCL1levels.In addition,the CXCL1-injected mouse model shortened the timeline for developing chronic PD mouse model to 2 weeks,offering an efficient platform for studying inflammation-driven processes in PD.The findings provide critical insights into the inflammatory mechanisms underlying PD and identify promising therapeutic targets for intervention.
基金supported by the National Research Foundation of Korea (NRF)funded by the Ministry of Science,ICT&Future Planning (2022R1A2C2006229,2022R1A6A3A01086868)Korea Dementia Research Project through the Korea Dementia Research Center (KDRC)funded by the Ministry of Health&Welfare and Ministry of Science and ICT,Republic of Korea (RS-2024-00345328)KIST Institutional Grant (2E32851)。
文摘Alzheimer'sdisease(AD)isaprogressive neurodegenerative disorder characterized by cognitive impairment and distinct neuropathological features,including amyloid-βplaques,neurofibrillary tangles,and reactive astrogliosis.Developing effective diagnostic,preventative,and therapeutic strategies for AD necessitates the establishment of animal models that accurately recapitulate the pathophysiological processes of the disease.Existing transgenic mouse models have significantly contributed to understanding AD pathology but often fail to replicate the complexity of human AD.Additionally,these models are limited in their ability to elucidate the interplay among amyloid-βplaques,neurofibrillary tangles,and reactive astrogliosis due to the absence of spatially and temporally specific genetic manipulation.In this study,we introduce a novel AD mouse model(APP/PS1-TauP301L-Adeno mice)designed to rapidly induce pathological symptoms and enhance understanding of AD mechanisms.Neurofibrillary tangles and severe reactive astrogliosis were induced by injecting AAVDJ-EF1a-hTauP301L-EGFP and Adeno-GFAP-GFP viruses into the hippocampi of 5-month-old APP/PS1 mice.Three months post-injection,these mice exhibited pronounced astrogliosis,substantial amyloid-βplaque accumulation,extensiveneurofibrillarytangles,accelerated neuronal loss,elevated astrocytic GABA levels,and significant spatial memory deficits.Notably,these pathological features were less severe in AAVTauP301L-expressing APP/PS1 mice without augmented reactive astrogliosis.These findings indicate an exacerbating role of severe reactive astrogliosis in amyloid-βplaque and neurofibrillary tangle-associated pathology.The APP/PS1-TauP301L-Adeno mouse model provides a valuable tool for advancing therapeutic research aimed at mitigating the progression of AD.
基金sponsored by the National Natural Science Foundation of China(Grant Nos.42175052 and U2442206)the Joint Research Project for Meteorological Capacity Improvement(Grant No.23NLTSQ007,23NLTSZ003)+2 种基金the Innovative Development Special Project of the China Meteorological Administration(Grant No.CXFZ2023J002)the National Key R&D Program of China(Grant No.2023YFC3007700,2024YFC3013100)the China Meteorological Administration Youth Innovation Team(Grant No.CMA2024QN06)。
文摘Based on the hindcasts from five subseasonal-to-seasonal(S2S)models participating in the S2S Prediction Project,this study evaluates the performance of the multimodel ensemble(MME)approach in predicting the subseasonal precipitation anomalies during summer in China and reveals the contributions of possible driving factors.The results suggest that while single-model ensembles(SMEs)exhibit constrained predictive skills within a limited forecast lead time of three pentads,the MME illustrates an enhanced predictive skill at a lead time of up to four pentads,and even six pentads,in southern China.Based on both deterministic and probabilistic verification metrics,the MME consistently outperforms SMEs,with a more evident advantage observed in probabilistic forecasting.The superior performance of the MME is primarily attributable to the increase in ensemble size,and the enhanced model diversity is also a contributing factor.The reliability of probabilistic skill is largely improved due to the increase in ensemble members,while the resolution term does not exhibit consistent improvement.Furthermore,the Madden–Julian Oscillation(MJO)is revealed as the primary driving factor for the successful prediction of summer precipitation in China using the MME.The improvement by the MME is not solely attributable to the enhancement in the inherent predictive capacity of the MJO itself,but derives from its capability in capturing the more realistic relationship between the MJO and subseasonal precipitation anomalies in China.This study establishes a scientific foundation for acknowledging the advantageous predictive capability of the MME approach in subseasonal predictions of summer precipitation in China,and sheds light on further improving S2S predictions.
文摘BACKGROUND At present,there is a lack of non-invasive indicators to evaluate the changes in endoscopic activity between two visits for patients with Crohn's disease(CD).AIM To develop a model for predicting whether endoscopic activity will improve in CD patients.METHODS This is a single-center retrospective study that included patients diagnosed with CD from January 2014 to December 2022.The patients were randomly divided into a modeling group(70%)and an internal validation group(30%),with an external validation group from January 2023 to March 2024.Univariate and binary logistic regression analyses were conducted to identify independent risk factors,which were used to construct a nomogram model.The model's performance was evaluated using receiver operating characteristic curves,calibration curves,and decision curve analysis(DCA).Additionally,further sensitivity analyses were performed.RESULTS One hundred seventy patients were included in the training group,while 64 were included in the external validation group.A binary logistic stepwise regression analysis revealed that the changes in the amplitudes of albumin(ALB)and fibrinogen(FIB)were independent risk factors for endoscopic improvement.A nomogram model was developed based on these risk factors.The area under the curve of the model for the training group,internal validation group,and external validation group were 0.802,0.788,and 0.787,respectively.The average absolute errors of the calibration curves were 0.011,0.016,and 0.018,respectively.DCA indicated that the model performs well in clinical practice.Additionally,sensitivity analysis demonstrated that the model has strong robustness and applicability.CONCLUSION Our study shows that changes in the amplitudes of ALB and FIB are effective predictors of endoscopic improvement in patients with CD during follow-up visits compared to their previous ones.
基金District of Medical and Health Research Project(2023JD212)Shenzhen Bao’an District of Traditional Chinese Medicine Clinical Research Project(2023ZYYLCZX-12)+2 种基金Shenzhen“Medical and Health Three Projects”Project Grant(SZZYSM 202106003)Weifang Health Committee Scientific Research Project(wfwsjk-2023-170)Shenzhen Pingshan District of Health System Research Project(2024334).
文摘Bacckground:Based on the 3C model,this study explores the current situation of HPV vaccine hesitancy among women of childbearing age and the factors influencing vaccine hesitancy.Methods:Based on the free cervical cancer screening project in Baoan District,this study designed a questionnaire under the framework of vaccine hesitation 3C theory and carried out a self-filling electronic questionnaire survey among women of childbearing age.Results:The rate of HPV vaccination awareness among women of childbearing age in Bao’an District was 93.25%.HPV vaccine acceptance reached 71.55%,and 24.59%of the survey respondents experienced HPV vaccine hesitation,a high percentage of whom were hesitant.The influencing factors of HPV vaccine hesitation among women of childbearing age were perceived necessity of HPV vaccination(0.482),no one around them receiving the HPV vaccine(0.411),perception of the price of the vaccine(0.354),degree of trust in the safety of the vaccine(0.223),and degree of concern about the HPV vaccine(0.153).Conclusion:The 3C model can be used for the study of HPV vaccine hesitancy.Strengthening the publicity of HPV vaccination and improving women’s knowledge of the HPV vaccine can reduce their hesitation toward HPV vaccination.
文摘To promote the global dissemination of Chinese culture,the translation of Chinese classic literature has garnered widespread attention in the translation field.Literary translation criticism plays an essential role in the development of translation endeavors.This paper will analyze three versions of English translations of Shen Congwen’s novella Border Town using Reiss’s translation criticism model and summarize their performance in terms of formal equivalence and aesthetic effect,linguistic components,and extra-linguistic components.
基金supported by the 2023 Youth Fund for Humanities and Social Sciences Research by the Ministry of Education of the People’s Republic of China(Grant No.23YJC740004).
文摘Based on BERTopic Model,the paper combines qualitative and quantitative methods to explore the reception of Can Xue’s translated works by analyzing readers’book reviews posted on Goodreads and Lovereading.We first collected book reviews from these two well-known websites by Python.Through topic analysis of these reviews,we identified recurring topics,including details of her translated works and appreciation of their translation quality.Then,employing sentiment and content analysis methods,the paper explored the emotional attitudes and the specific thoughts of readers toward Can Xue and her translated works.The fingdings revealed that,among the 408 reviews,though the reception of Can Xue’s translated works was relatively positive,the current level of attention and recognition remains insufficient.However,based on the research results,the paper can derive valuable insights into the translation and dissemination processes such as adjusting translation and dissemination strategies,so that the global reach of Chinese literature and culture can be better facilitated.
基金supported by the National Natural Science Foundation of China(grant No.81971380 and No.82301822).
文摘Peyronie’s disease(PD)is a disorder characterized by fibrous plaque formation in the penile tissue that leads to curvature and complications in advanced stages.In this study,we aimed to compare four injectable induction agents for the establishment of a robust rat model of PD:transforming growth factor-β1(TGF-β1),fibrin,sodium tetradecyl sulfate(STS)combined with TGF-β1,and polidocanol(POL)combined with TGF-β1.The results showed that injection of TGF-β1 or fibrin into the tunica albuginea induced pathological endpoints without causing penile curvature.The STS+TGF-β1 combination resulted in both histological and morphological alterations,but with a high incidence of localized necrosis that led to animal death.The POL+TGF-β1 combination produced pathological changes and curvature comparable to STS+TGF-β1 and led to fewer complications.In conclusion,fibrin,STS+TGF-β1,and POL+TGF-β1 all induced PD with a certain degree of penile curvature and histological fibrosis in rats.The POL+TGF-β1 combination offered comparatively greater safety and clinical relevance and may have the greatest potential for PD research using model rats.
文摘This paper,grounded in the theory of business model innovation,examines the Chinese People’s Health Press(人民卫生出版社)as a case study to explore strategies for innovating business models within the context of professional publishing convergence development.The research posits that effective business model innovation in this domain necessitates a comprehensive reform of its constituent elements.It advocates for a systematic approach to reconstructing value propositions,enhancing value creation and delivery processes,and optimizing value capture mechanisms to achieve desired outcomes.
基金Education and Teaching Research Project of Beijing University of Technology(ER2024KCB08)。
文摘With the widespread application of large language models(LLMs)in natural language processing and code generation,traditional High-Level Language Programming courses are facing unprecedented challenges and opportunities.As a core programming language for computer science majors,C language remains irreplaceable due to its foundational nature and engineering adaptability.This paper,based on the rapid development of large model technologies,proposes a systematic reform design for C language teaching,focusing on teaching objectives,content structure,teaching methods,and evaluation systems.The article suggests a teaching framework centered on“human-computer collaborative programming,”integrating prompt training,AI-assisted debugging,and code generation analysis,aiming to enhance students’problem modeling ability,programming expression skills,and AI collaboration literacy.
基金Supported by the National Natural Science Foundation of China under Grants 61631010 and 61806085.
文摘High-fidelity tactile rendering offers significant potential for improving the richness and immersion of touchscreen interactions.This study focuses on a quantitative description of tactile rendering fidelity using a custom-designed hybrid electrovibration and mechanical vibration(HEM)device.An electrovibration and mechanical vibration(EMV)algorithm that renders 3D gratings with different physical heights was proposed and shown to achieve 81%accuracy in shape recognition.Models of tactile rendering fidelity were established based on the evaluation of the height discrimination threshold,and the psychophysical-physical relationships between the discrimination and reference heights were well described by a modification of Weber’s law,with correlation coefficients higher than 0.9.The physiological-physical relationship between the pulse firing rate and the physical stimulation voltage was modeled using the Izhikevich spiking model with a logarithmic relationship.
基金supported by Shanghai 2021“Science and Technology Innovation Action Plan”:Social Development Science and Technology Research Project(Grant No.21DZ1202703).
文摘The numerical simulation of the fluid flow and the flexible rod(s)interaction is more complicated and has lower efficiency due to the high computational cost.In this paper,a semi-resolved model coupling the computational fluid dynamics and the flexible rod dynamics is proposed using a two-way domain expansion method.The gov-erning equations of the flexible rod dynamics are discretized and solved by the finite element method,and the fluid flow is simulated by the finite volume method.The interaction between fluids and solid rods is modeled by introducing body force terms into the momentum equations.Referred to the traditional semi-resolved numerical model,an anisotropic Gaussian kernel function method is proposed to specify the interactive forces between flu-ids and solid bodies for non-circle rod cross-sections.A benchmark of the flow passing around a single flexible plate with a rectangular cross-section is used to validate the algorithm.Focused on the engineering applications,a test case of a finite patch of cylinders is implemented to validate the accuracy and efficiency of the coupled model.
基金the framework of the state assignment of Keldysh Institute of Applied Mathematics of RAS(Project No.125020701776-0)the Ministry of Education and Science of Russia for IO RAS(Project No.FMWE-2024-0018).
文摘The work considers the problem of gas hydrate dissociation in a porous medium using the two-term Forchheimer law,corresponding to high flow rates of reservoir fluids.Such rates can arise during the decomposition of gas hydrates,since a large amount of gas is released.Intensive emissions of gases from the earth’s interior are observed on the ocean floor.They are also associated with a large number of subvertical geological structures under the ocean floor,coming to the surface in the formof local ring funnels(pockmarks).Many similar objects have also been found on land.Particular interest in this problemis caused by climate threats associated with the release of greenhouse gases.The movement of gas released fromthe hydrate to the breakthrough channel is similar to the gas inflow to the well(without hydrate),which is usually described by a two-term filtration law.In this work,a mathematical model of gas hydrate dissociation with a nonlinear Forchheimer-type law ofmotion is developed.The systemis split in two blocks by physical processes,taking into account the quadratic correction to the velocity in the filtration law.The first block is responsible for the convective transfer of saturation parameters in the model,water,gas and hydrate saturations are taken into account.The second block corresponds to the equation of dissipative piezoconductivity with a different number of thermodynamic degrees of freedom,taking into account heat and mass transfer in a porous medium.The performed splitting allows using explicit-implicit difference schemes when solving problems and avoiding strong refinement of the step in time and space.For numerical modeling,the support operator method is used,which makes it possible to discretize partial differential equations on irregular grids,which allows taking into account the complex geometry and lithology of the reservoir.A difference scheme based on the support operator method is developed,which,due to the mutually consistent approximation of vector analysis operations(divergence and gradient),allows to take into account the various flux laws between adjacent grid cells,including quadratic corrections to the velocity.Based on the developed numerical algorithms and their program implementations,calculations of gas hydrate dissociation are performed both in a reservoir of simple geometric structure and in a heterogeneous reservoir of complex configuration.The results obtained correspond to the physics of the processes under consideration.
文摘Against the backdrop of globalization and regional economic integration,border tourism,as a unique form of tourism,is increasingly emerging as a significant driving force for promoting economic development in border regions,fostering cultural exchange,and strengthening international cooperation.The northwestern Yunnan region,located in China’s Southwestern frontier,is a key area bordering Southeast and South Asian countries,endowed with abundant border tourism resources.This article delves into the border tourism models and pathways for border development and prosperity in ethnic regions,using a case study of the Northwest Yunnan region to analyze the mechanisms and influencing factors of border tourism in promoting local economic development,social progress,and cultural heritage.This provides empirical evidence for further understanding the development patterns of border tourism and also contributes to strengthening ethnic unity and maintaining harmony and stability in border regions.This study addresses the current research gap in exploring the development models and pathways of border tourism in specific regions,offering valuable references and insights for future related research.
基金supported by grants from“2023 Research on Vocational English Curriculum Reform:‘Implementation Path of Ideological and Political Education in Vocational English Courses for Higher Vocational Education Under the New Curriculum Standards’(Grant No.WYJZW-2023GD0029)”“The Special Project of 2024 on‘Reading and Teacher Development’Under the 14th Five-Year Plan of the China Tao Xingzhi Research Association(Grant No.CTXZRA202411937GD)”.
文摘Using the“Civil Aviation Oral English”course as a case study,we operationalize the ideological and political education(IPE)integrated evaluation system for oral English courses in higher vocational education,which is based on Kirkpatrick’s Model,into a tripartite teaching evaluation system.The system encompasses three components such as learning objective attainment,individual developmental progress,and value-added competency assessment.The proposed system facilitates three-dimensional,multi-level,and scientifically validated teaching evaluation.This approach aligns with the holistic development of students’knowledge structures,professional competencies,moral qualities,and emotional intelligence.The study provides novel methodological perspectives for IPE evaluation in vocational oral English instruction,particularly in:curriculum-based ideological mapping,competency growth metrics,and value internalization assessment.
基金Ongoing Research Funding program(ORF-2025-1404),King Saud University,Riyadh,Saudi Arabia。
文摘Chikungunya is a mosquito-borne viral infection caused by the chikungunya virus(CHIKV).It is characterized by acute onset of high fever,severe polyarthralgia,myalgia,headache,and maculopapular rash.The virus is rapidly spreading and may establish in new regions where competent mosquito vectors are present.This research analyzes the regulatory dynamics of a stochastic differential equation(SDE)model describing the transmission of the CHIKV,incorporating seasonal variations,immunization efforts,and environmentalffuctuations modeled through Poisson random measure noise under demographic heterogeneity.The model guarantees the existence of a global positive solution and demonstrates periodic dynamics driven by environmental factors.A key contribution of this study is the formulation of a stochastic threshold parameter,R0L,which characterizes the conditions for disease persistence or extinction under random environmental inffuences.Although our analysis highlights age-speciffc heterogeneities to illustrate differential transmission risks,the framework is general and can incorporate other vulnerable demographic groups,ensuring broader applicability of the results.Using the Monte Carlo Markov Chain(MCMC)method,we estimate R0L=1.4978(95%C-I:1.4968–1.5823)based on CHIKV data from Florida,USA,spanning 2005 to 2017,suggesting that the outbreak remains active and requires targeted control strategies.The effectiveness of immunization,screening,and treatment strategies varies depending on the prioritized demographic groups,due to substantial differences in CHIKV incidence across age categories in the USA.Numerical simulations were conducted using the truncated Euler–Maruyama method to robustly capture the stochastic dynamics of CHIKV transmission with Poissondriven jumps.Employing an iterative approach and assuming mild convexity conditions,we formulated and solved a parameterized near-optimality problem using the Ekeland variational principle.Ourffndings indicate that vaccination campaigns are signiffcantly more effective when focused on vulnerable adults over the age of 66,as well as individuals aged 21 to 25.Furthermore,enhancements in vaccine effcacy,diagnostic screening,and treatment protocols all contribute substantially to minimizing infection rates compared to current standard approaches.These insights support the development of targeted,age-speciffc public health interventions that can signiffcantly improve the management and control of future CHIKV outbreaks.
文摘Driven by the dual carbon goals and the national strategy for the high-quality development of the advanced manufacturing industry,along with the trend of economic transformation,China’s new energy vehicle market has experienced explosive growth,which has spurred a surge in the construction of domestic charging infrastructure.Charging infrastructure not only supports achieving dual carbon goals and the development of the new energy vehicle industry but also undertakes the new historical mission of infrastructure in China’s new development stage,becoming a crucial infrastructure connecting energy decarbonization and transportation electrification[1].In recent years,central and local governments have placed great emphasis on this field.They have introduced policies from various angles,including comprehensive macro-level measures,government planning,financial subsidies,charging rates,industry management,and scientific and technological innovation,to promote the moderately advanced development of charging facilities.
基金Supported by Amrita Vishwa Vidyapeetham Seed Grant,No.K-PHAR-24-722DST INSPIRE Fellowship,No.IF190226.
文摘BACKGROUND Syngeneic orthotopic tumor models offer an optimal functional tumor–immune interface for hepatocellular carcinoma research.Yet,unpredictable growth kinetics and spontaneous regression pose major obstacles.Efficient induction protocols and continuous monitoring are therefore essential.Routine exploratory surgeries are ethically untenable,making non-invasive imaging modalities attractive alternatives.High-resolution magnetic resonance imaging and microcomputed tomography deliver detailed insights but incur substantial equipment costs,radiation risks,time demands,and require specialized expertise—challenges that limit their routine use.In contrast,ultrasound(US)imaging emerges as a cost-effective,radiation-free,and rapid approach,facilitating practical and ethical longitudinal assessment of tumor progression in preclinical studies.AIM To optimize the orthotopic hepatocellular carcinoma model and evaluate the potential of US imaging for accurate and cost-effective tumor monitoring.METHODS Hepatocellular carcinoma was induced in 28 Sprague Dawley rats by implanting 5×10^(6) N1S1 cells into the left lateral hepatic lobe.Tumor progression was monitored weekly via US.Upon reaching 100-150 mm^(3),an experimental group(n=14)received Sorafenib(40 mg/kg)orally on alternate days for 28 days;efficacy was compared to untreated controls.US accuracy was validated against micro-computed tomography,gross caliper measurements and histopathological analysis.Reliability and operator proficiency in US assessment were also evaluated.RESULTS US images procured 7-day post-surgery revealed a well-defined hypoechoic nodule at the left liver lobe tip,confirming successful tumor induction(mean volume 130±39 mm^(3)).Only three animals exhibited spontaneous regression by week 2,underscoring the model’s stability.Sorafenib treatment elicited a marked tumor reduction(678±103 mm^(3))vs untreated control(6005±1760 mm^(3)).US assessment demonstrated robust intra and interobserver reproducibility with high sensitivity and specificity for tumor detection.Moreover,US derived volumes correlated strongly with gross caliper measurements,histopathological analysis,and microcomputed tomography imaging,validating its reliability as a non-invasive monitoring tool in preclinical hepatocellular carcinoma studies.CONCLUSION The results demonstrate that US imaging is a reliable,cost-effective,and animal sparing approach with an easy tomaster protocol,enabling monitoring of tumor progression and therapeutic response in orthotopic liver tumor models.
