Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(...Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(%)were calculated by Celltiter-GLO Assay for evaluating CC50,EC50 and SI values.To observe the drug resistance of the virus to different concentrations of Hehuan Yin within 72 hours by detecting luciferase activity,western-blot was used to detect the protein expression levels of NS5A,NS3 and NS5B.Results:the CC50,EC50 and SI of Hehuan Yin against HCV2a were 132.50g/ml,1.90g/ml and 67.90 respectively.The EC50 after 24h,48h and 72h administration were 18g/ml,5.8g/ml and 2.3g/ml respectively.Within the range of drug concentration,the aqueous extract Hehuan Yin had inhibitory effect on the expression of NS5A and NS5B proteins in a dose-effect relationship,but had no obvious effect on the expression of NS3 protein.Conclusion:The aqueous extract of Hehuan Yin may inhibit the replication of HCV2a virus by changing the protein expression levels of NS5A and NS5B,and the virus has no tolerance to the aqueous extract of Hehuan Yin.展开更多
Optical heterodyne magnetic rotation enhanced velocity modulation spectroscopy was employed to observe thevisible absorption spectra of the B2∑u+ -X2∑g^ + electronic transition of C2-. Four hot bands (0,1), (1,2), (...Optical heterodyne magnetic rotation enhanced velocity modulation spectroscopy was employed to observe thevisible absorption spectra of the B2∑u+ -X2∑g^ + electronic transition of C2-. Four hot bands (0,1), (1,2), (2,3) and (3,4)have been observed and the band (3,4) is measured directly for the first time, so far as we know, by absorption. Arotational analysis was carried out to obtain molecular constants. With the Franck-Condon principle and the vibrationalBoltzmann distribution, we have estimated the vibrational temperature of C2- to be about 3000K.展开更多
After satellite deployment, the second stage of a LM-2C launch vehicle performing a SSO mission has an orbital life of over 50 years. It is therefore necessary to vent the residual propellants in the 2nd stage to avoi...After satellite deployment, the second stage of a LM-2C launch vehicle performing a SSO mission has an orbital life of over 50 years. It is therefore necessary to vent the residual propellants in the 2nd stage to avoid debris-generating in-orbit explosion. Based on the characteristics of LM-2C, several concepts have been proposed. After detailed evaluation of these concepts, the method to drain the residual propellants through the main valves of the main engine has been selected.展开更多
等离子体处理作为一种高效的表面改性技术,在二维纳米材料/碳基复合结构的形貌调控中具有独特优势。本文通过调控等离子体处理参数(例如气体氛围),研究其对二硫化钨/碳(WS2/C)复合结构表面形貌和缺陷分布的影响。结合扫描电子显微镜(Sca...等离子体处理作为一种高效的表面改性技术,在二维纳米材料/碳基复合结构的形貌调控中具有独特优势。本文通过调控等离子体处理参数(例如气体氛围),研究其对二硫化钨/碳(WS2/C)复合结构表面形貌和缺陷分布的影响。结合扫描电子显微镜(Scanning Electron Microscope,SEM)和基于拉曼光谱的表征,揭示了等离子体处理诱导的WS2边缘选择性和碳纳米纤维骨架的协同效应,提高了复合材料的比表面积和边缘活性位点暴露程度。试验结果表明,参数优化后,等离子体处理可以有效调控WS2纳米晶片在碳纤维骨架中的分散性,并抑制晶片层间堆叠和层片团聚,从而增加边缘位置的暴露程度。该研究为高性能WS2/C复合材料的可控制备提供了新思路,推动其在能源存储与转换领域的实际应用。展开更多
基金Natural Foundation of Guangdong Province(No.2018A030313308)。
文摘Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(%)were calculated by Celltiter-GLO Assay for evaluating CC50,EC50 and SI values.To observe the drug resistance of the virus to different concentrations of Hehuan Yin within 72 hours by detecting luciferase activity,western-blot was used to detect the protein expression levels of NS5A,NS3 and NS5B.Results:the CC50,EC50 and SI of Hehuan Yin against HCV2a were 132.50g/ml,1.90g/ml and 67.90 respectively.The EC50 after 24h,48h and 72h administration were 18g/ml,5.8g/ml and 2.3g/ml respectively.Within the range of drug concentration,the aqueous extract Hehuan Yin had inhibitory effect on the expression of NS5A and NS5B proteins in a dose-effect relationship,but had no obvious effect on the expression of NS3 protein.Conclusion:The aqueous extract of Hehuan Yin may inhibit the replication of HCV2a virus by changing the protein expression levels of NS5A and NS5B,and the virus has no tolerance to the aqueous extract of Hehuan Yin.
文摘Optical heterodyne magnetic rotation enhanced velocity modulation spectroscopy was employed to observe thevisible absorption spectra of the B2∑u+ -X2∑g^ + electronic transition of C2-. Four hot bands (0,1), (1,2), (2,3) and (3,4)have been observed and the band (3,4) is measured directly for the first time, so far as we know, by absorption. Arotational analysis was carried out to obtain molecular constants. With the Franck-Condon principle and the vibrationalBoltzmann distribution, we have estimated the vibrational temperature of C2- to be about 3000K.
文摘After satellite deployment, the second stage of a LM-2C launch vehicle performing a SSO mission has an orbital life of over 50 years. It is therefore necessary to vent the residual propellants in the 2nd stage to avoid debris-generating in-orbit explosion. Based on the characteristics of LM-2C, several concepts have been proposed. After detailed evaluation of these concepts, the method to drain the residual propellants through the main valves of the main engine has been selected.
文摘等离子体处理作为一种高效的表面改性技术,在二维纳米材料/碳基复合结构的形貌调控中具有独特优势。本文通过调控等离子体处理参数(例如气体氛围),研究其对二硫化钨/碳(WS2/C)复合结构表面形貌和缺陷分布的影响。结合扫描电子显微镜(Scanning Electron Microscope,SEM)和基于拉曼光谱的表征,揭示了等离子体处理诱导的WS2边缘选择性和碳纳米纤维骨架的协同效应,提高了复合材料的比表面积和边缘活性位点暴露程度。试验结果表明,参数优化后,等离子体处理可以有效调控WS2纳米晶片在碳纤维骨架中的分散性,并抑制晶片层间堆叠和层片团聚,从而增加边缘位置的暴露程度。该研究为高性能WS2/C复合材料的可控制备提供了新思路,推动其在能源存储与转换领域的实际应用。
