Objective:To investigate activity-guided isolation and identification of anti-Staphylococcus aures components from Senecio tenuifolius Burm.F.(S.tenuifolius).Methods:Hexane,chloroform,ethyl acetate,methanol and aqueou...Objective:To investigate activity-guided isolation and identification of anti-Staphylococcus aures components from Senecio tenuifolius Burm.F.(S.tenuifolius).Methods:Hexane,chloroform,ethyl acetate,methanol and aqueous extracts of S.tenuifolius were prepared by soxilation for antimicrobial activity against one registered Staphylococcus aureus(S.aureus)(ATCC No:25923)and two clinical isolates,methicillin resistant and methicillin sensitive S.aureus.NCCL standard methods were followed for antibacterial activity.GC-MS was performed to identify the chemical composition of bio active fraction.Results:Among all solvent extracts,methanol extract significantly reduced the growth of S.aureus(ATCC No:25923),methicillin resistant and methicillin sensitive S.aureus with the best zone of inhibition at 16.23,14.06 and 15.23mm and minimum inhibition concentration(MIC)values at 426.16,683.22 and 512.12μg/mL,respectively.In order to detect the active component in methanol extract,it was further purified by column chromatography,which yielded four fractions(St1,St2,St3,and St4).Among these four fractions,St3 was effective against the tested strains of S.aures,with the best zone of inhibition at 15.09,13.25 and 14.12 mm and with best MIC values at 88.16,128.11 and 116.12μg/mL,respectively.Effective fraction partially purified from S.tenuifolius(St3)yielded MIC's that were at least 20 fold less when compared to crude extract.GC-MS analysis of St3 revealed the presence of 3-[methyl-6,7-dihydro benzofuran-4(5H)-one],1,2-benzenedicarboxylic acid,hydroquinone,methyl ester and 3 unknown compounds.Conclusions:The study provides scientific evidence for traditional and folklore medicinal use of S.tenuifolius in skin infections treatment.展开更多
Objective:To evaluate the effects of n-hexane insoluble fraction(HIF)of Ficus septica leaves in combination with doxorubicin on cytotoxicity,cell cycle and apoptosis induction of breast cancer T47D cell lines.Methods:...Objective:To evaluate the effects of n-hexane insoluble fraction(HIF)of Ficus septica leaves in combination with doxorubicin on cytotoxicity,cell cycle and apoptosis induction of breast cancer T47D cell lines.Methods:The in vitro drugs-stimulated cytotoxic effects were determined using MTT assay.Analysis of cell cycle distribution was performed using flowcytometer and the data was analyzed using ModFit LT 3.0 program.Apoptosis assay was earned out by double staining method using ethydium bromide-acridin orange.The expression of cleaved-poly(ADP-ribose)polymerase(PARP)on T47D cell lines was identified using immunocytochemistry.Results:The combination exhibited higher inhibitory effect on cell growth than the single treatment of doxorubicin in T47D cells.In addition,combination of doxorubicin and HIF increased the incidence of cells undergoing apoptosis.HIF could improve doxorubicin cytotoxic effect by changing the accumulation of cell cycle phase from G_2/M to G_1 phase.The combination also exhibited upregulation of cleaved-PARP in T47D cells.Conclusions:Based on this results,HIF is potential to be developed as co-chemotherapeutic agent for breast cancer by inducing apoptosis and cell cycle arrest.However,the molecular mechanism need to be explored further.展开更多
目的:建立测定穿心莲叶中常用的4种农药残留量的方法,更好地控制药材的质量。方法:以灭蚜威为测定内标,样品经乙腈提取,采用Atlantics d C18柱分离,液相色谱-串联质谱联用(LC—MS/MS)方法测定穿心莲药材中常用的4种农药残留量。结果:4...目的:建立测定穿心莲叶中常用的4种农药残留量的方法,更好地控制药材的质量。方法:以灭蚜威为测定内标,样品经乙腈提取,采用Atlantics d C18柱分离,液相色谱-串联质谱联用(LC—MS/MS)方法测定穿心莲药材中常用的4种农药残留量。结果:4种农药检测限在0.003～0.0093 mg·kg-1之间,回收率在83%～112%之间,回收率相对标准偏差均低于6%。结论:方法简便、灵敏、重现性好,能满足同时测定4种农药在穿心莲药材中的残留量。展开更多
基金Supported by the Department of Riotechnology.Government of Indiafor funding(DBT-Sanction No.102/IFD/SAN/PR0313)
文摘Objective:To investigate activity-guided isolation and identification of anti-Staphylococcus aures components from Senecio tenuifolius Burm.F.(S.tenuifolius).Methods:Hexane,chloroform,ethyl acetate,methanol and aqueous extracts of S.tenuifolius were prepared by soxilation for antimicrobial activity against one registered Staphylococcus aureus(S.aureus)(ATCC No:25923)and two clinical isolates,methicillin resistant and methicillin sensitive S.aureus.NCCL standard methods were followed for antibacterial activity.GC-MS was performed to identify the chemical composition of bio active fraction.Results:Among all solvent extracts,methanol extract significantly reduced the growth of S.aureus(ATCC No:25923),methicillin resistant and methicillin sensitive S.aureus with the best zone of inhibition at 16.23,14.06 and 15.23mm and minimum inhibition concentration(MIC)values at 426.16,683.22 and 512.12μg/mL,respectively.In order to detect the active component in methanol extract,it was further purified by column chromatography,which yielded four fractions(St1,St2,St3,and St4).Among these four fractions,St3 was effective against the tested strains of S.aures,with the best zone of inhibition at 15.09,13.25 and 14.12 mm and with best MIC values at 88.16,128.11 and 116.12μg/mL,respectively.Effective fraction partially purified from S.tenuifolius(St3)yielded MIC's that were at least 20 fold less when compared to crude extract.GC-MS analysis of St3 revealed the presence of 3-[methyl-6,7-dihydro benzofuran-4(5H)-one],1,2-benzenedicarboxylic acid,hydroquinone,methyl ester and 3 unknown compounds.Conclusions:The study provides scientific evidence for traditional and folklore medicinal use of S.tenuifolius in skin infections treatment.
基金Supported by DP2M DIKT1(Directorate of Higher Education) Ministry of EducationIndonesia through "Hibah Bersaing" Research Grant 2011
文摘Objective:To evaluate the effects of n-hexane insoluble fraction(HIF)of Ficus septica leaves in combination with doxorubicin on cytotoxicity,cell cycle and apoptosis induction of breast cancer T47D cell lines.Methods:The in vitro drugs-stimulated cytotoxic effects were determined using MTT assay.Analysis of cell cycle distribution was performed using flowcytometer and the data was analyzed using ModFit LT 3.0 program.Apoptosis assay was earned out by double staining method using ethydium bromide-acridin orange.The expression of cleaved-poly(ADP-ribose)polymerase(PARP)on T47D cell lines was identified using immunocytochemistry.Results:The combination exhibited higher inhibitory effect on cell growth than the single treatment of doxorubicin in T47D cells.In addition,combination of doxorubicin and HIF increased the incidence of cells undergoing apoptosis.HIF could improve doxorubicin cytotoxic effect by changing the accumulation of cell cycle phase from G_2/M to G_1 phase.The combination also exhibited upregulation of cleaved-PARP in T47D cells.Conclusions:Based on this results,HIF is potential to be developed as co-chemotherapeutic agent for breast cancer by inducing apoptosis and cell cycle arrest.However,the molecular mechanism need to be explored further.
文摘目的:建立测定穿心莲叶中常用的4种农药残留量的方法,更好地控制药材的质量。方法:以灭蚜威为测定内标,样品经乙腈提取,采用Atlantics d C18柱分离,液相色谱-串联质谱联用(LC—MS/MS)方法测定穿心莲药材中常用的4种农药残留量。结果:4种农药检测限在0.003～0.0093 mg·kg-1之间,回收率在83%～112%之间,回收率相对标准偏差均低于6%。结论:方法简便、灵敏、重现性好,能满足同时测定4种农药在穿心莲药材中的残留量。
