A new saikosaponin was isolated from Bupleurum chinense DC., and its structure was identified as 3β,16α,23,28,30_pentahydroxy_olean_11,13(18)_dien_3_O_β_D_glucopyranosyl(1→6)_[α_L_rhamnopyranosyl (1→4)]_β_D...A new saikosaponin was isolated from Bupleurum chinense DC., and its structure was identified as 3β,16α,23,28,30_pentahydroxy_olean_11,13(18)_dien_3_O_β_D_glucopyranosyl(1→6)_[α_L_rhamnopyranosyl (1→4)]_β_D_glucopyranoside on the basis of chemical and spectral evidence, named as saikosaponin q_1. In addition, two known saikosaponins, 3″_O_acetyl_saikosaponin d and 3″_O_acetyl_saikosaponin b 2, were also isolated and identified from this plant for the first time.展开更多
To facilitate the species identification and quality assessment of Chaihu （Bupleuri Radix）, a simple and valid chromatographic fingerprint method was developed. The method uses high-performance liquid chromatography...To facilitate the species identification and quality assessment of Chaihu （Bupleuri Radix）, a simple and valid chromatographic fingerprint method was developed. The method uses high-performance liquid chromatography coupled with evaporative light scattering detector （HPLC-ELSD） and the data analysis is assisted by professional analytical software recommended by the State Food and Drug Administration （SFDA）. The results indicate that Nan Chaihu raw materials and Chaihu decoction pieces vary markedly in chemical quality, while Bei Chaihu raw materials are relatively more stable. Furthermore, it is obvious that Nan Chaihu is chemically very different from Bei Chaihu, suggesting that Nan Chaihu may not be suitable for medicinal use. In addition, the obvious differences between the chromatograms of decoction pieces and raw materials, especially the peaks between 30 and 40 rain and after 45 rain, indicate possible effects of the processing procedures on the chemicals. By analyzing the fingerprints of all samples, 12 main saponin-like fingerprint peaks, of which at least three are characteristic peaks of saikosaponins a, c, and d, are proposed to be considered for further characterization and quality evaluation of Chaihu.展开更多
In China,some species of Bupleurum (family Umbelliferae) have been used as Chinese traditional medicine under the name “Chai-Hu” for the treatment of fevers and influenza.In the present paper,the authors report a st...In China,some species of Bupleurum (family Umbelliferae) have been used as Chinese traditional medicine under the name “Chai-Hu” for the treatment of fevers and influenza.In the present paper,the authors report a study on the investigation and exploitation of new medicinal resources of “Chai-Hu” in Qinghai Province and Xizang (Tibet) Autonomous Region where no one has studied this hitherto.18 species,7 varieties,I form of Bupleurum and a new species (Bupleurum qinghaiense) have been discovered.The taxonomy and preliminary phytochemical studies of the roots of three medicinal plants,B.qinghaiense,B.rnarginatum var.stenophyllum and B.smithii var.parvifolium.are reported in this paper.展开更多
Bupleurum polysaccharides(BPs)is isolated from Bupleurum smithii var.parvifolium,a key traditional Chinese medicine.The study was to investigate the effects of BPs on diabetic kidney injury.After two intraperitoneal i...Bupleurum polysaccharides(BPs)is isolated from Bupleurum smithii var.parvifolium,a key traditional Chinese medicine.The study was to investigate the effects of BPs on diabetic kidney injury.After two intraperitoneal injections of streptozotozin(STZ)100 mg·kg^–1,renal injury in diabetic mice was induced and BPs was orally administrated at dosages of 30 and 60 mg·kg^–1·d^–1.The STZ injected mice developed renal function damage,renal inflammation and fibrosis known as diabetic kidney disease(DKD).BPs significantly reduced serum creatinine level and urinary albumin excretion rate,with the attenuated swelling of kidneys.BPs treatment obviously alleviated the pathological damage of renal tissue.The progression of renal injury in BPs treated mice was inhibited with less expression of type IV collagen(Col IV),fibronectin(FN)andα-smooth muscle actin(α-SMA).The inhibition of inflammation in kidney was associated with the reduced level of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).BPs administration suppressed the over-expression of toll like receptor 4(TLR4)and high-mobility group box 1(HMGB1)with lowered activity of nuclear factor kappa B(NF-κB)in renal tissue of diabetic mice.Oral administration of BPs effectively prevented the development of renal injury in diabetic mice.This study suggested that the protection provided by BPs might affect through the interruption of HMGB1-TLR4 pathway,leading to the inhibition of renal inflammation and fibrotic process.展开更多
The present study aimed to investigate the effects of polysaccharides extracted from Bupleurum chinense DC(BCPs) on macrophage functions. In the in vivo experiment, 1 m L of 5% sodium thioglycollate was injected into ...The present study aimed to investigate the effects of polysaccharides extracted from Bupleurum chinense DC(BCPs) on macrophage functions. In the in vivo experiment, 1 m L of 5% sodium thioglycollate was injected into the abdomen of the mice on Day 0 and macrophages were harvested on Day 4. The macrophages were cultured in plates and treated with different concentrations of BCPs and stimulus. Effects of BCPs on macrophage functions were assessed by chemotaxis assay, phagocytosis assay and Enzyme-Linked Immunosorbent Assay(ELISA). Our results showed the enhanced chemotaxis, phagocytosis and secretion of nitric oxide(NO) and inflammatory cytokines by macrophages when treated with BCPs. However, when chemotaxis and phagocytosis were up-regulated by complement components or opsonized particles, BCPs inhibited these effects. Also, the NO production induced by lipopolysaccharides(LPS) was suppressed by BCPs mildly. Moreover, BCPs had an inhibitory effect on the [Ca^(2+)]i elevation of macrophages. These results suggested that BCPs exerted modulatory effects on macrophage functions, which may contribute to developing novel approaches to treating inflammatory diseases.展开更多
As a traditional Chinese herbal medicine exhibiting analgesic,fever-reducing and anti-inflammatory effects,Radix Bupleuri（Chai-Hu） is commonly used for the treatment of influenza,which is derived from the dried root...As a traditional Chinese herbal medicine exhibiting analgesic,fever-reducing and anti-inflammatory effects,Radix Bupleuri（Chai-Hu） is commonly used for the treatment of influenza,which is derived from the dried roots of Bupleurum chinense DC.and Bupleurum scorzonerifolium Willd.Among of diverse chemical components,saikosaponins are the key active components of the herb medicine.In the present study,we established a method of high performance liquid chromatography（HPLC） coupled with evaporative light scattering detection（ELSD） for simultaneous determination of saikosaponin a,c and d in root,stem,leaf and flower of Bupleurum chinense（B chinense） collected from different areas of Shanxi Province,China.The results from 16 samples of root,stem,leaf and flower of B chinense demonstrated that the total contents of the three saikosaponins in the root of B chinense collected from Dongshan Taiyuan,Xishan,Tianlongshan and Pangquangou were 4.26 mg/g,3.22 mg/g,4.23 mg/g and 3.05 mg/g,respectively.However,there was scarcely any saikosaponins in the stem,leaf and flower of B chinense collected from above-mentioned areas.The method of HPLC coupled with ELSD was suitable for quality control of Radix Bupleuri.The result also confirmed that the root of B chinense was the best medicinal part.展开更多
文摘A new saikosaponin was isolated from Bupleurum chinense DC., and its structure was identified as 3β,16α,23,28,30_pentahydroxy_olean_11,13(18)_dien_3_O_β_D_glucopyranosyl(1→6)_[α_L_rhamnopyranosyl (1→4)]_β_D_glucopyranoside on the basis of chemical and spectral evidence, named as saikosaponin q_1. In addition, two known saikosaponins, 3″_O_acetyl_saikosaponin d and 3″_O_acetyl_saikosaponin b 2, were also isolated and identified from this plant for the first time.
基金The project of Quality Standards for Chinese Medicines and Information System Platform (Grant No.2009ZX09308004)National Natural Science Foundation of China (Grant No.21172008)
文摘To facilitate the species identification and quality assessment of Chaihu （Bupleuri Radix）, a simple and valid chromatographic fingerprint method was developed. The method uses high-performance liquid chromatography coupled with evaporative light scattering detector （HPLC-ELSD） and the data analysis is assisted by professional analytical software recommended by the State Food and Drug Administration （SFDA）. The results indicate that Nan Chaihu raw materials and Chaihu decoction pieces vary markedly in chemical quality, while Bei Chaihu raw materials are relatively more stable. Furthermore, it is obvious that Nan Chaihu is chemically very different from Bei Chaihu, suggesting that Nan Chaihu may not be suitable for medicinal use. In addition, the obvious differences between the chromatograms of decoction pieces and raw materials, especially the peaks between 30 and 40 rain and after 45 rain, indicate possible effects of the processing procedures on the chemicals. By analyzing the fingerprints of all samples, 12 main saponin-like fingerprint peaks, of which at least three are characteristic peaks of saikosaponins a, c, and d, are proposed to be considered for further characterization and quality evaluation of Chaihu.
文摘In China,some species of Bupleurum (family Umbelliferae) have been used as Chinese traditional medicine under the name “Chai-Hu” for the treatment of fevers and influenza.In the present paper,the authors report a study on the investigation and exploitation of new medicinal resources of “Chai-Hu” in Qinghai Province and Xizang (Tibet) Autonomous Region where no one has studied this hitherto.18 species,7 varieties,I form of Bupleurum and a new species (Bupleurum qinghaiense) have been discovered.The taxonomy and preliminary phytochemical studies of the roots of three medicinal plants,B.qinghaiense,B.rnarginatum var.stenophyllum and B.smithii var.parvifolium.are reported in this paper.
基金funded by the National Natural Science Foundation of China(Nos.81673658,81274165 and 30925042)Ministry of Science and Technology of China(No.2018ZX09735003-002)the Science and Technology Commission of Shanghai Municipality(No.12JC1400800)
文摘Bupleurum polysaccharides(BPs)is isolated from Bupleurum smithii var.parvifolium,a key traditional Chinese medicine.The study was to investigate the effects of BPs on diabetic kidney injury.After two intraperitoneal injections of streptozotozin(STZ)100 mg·kg^–1,renal injury in diabetic mice was induced and BPs was orally administrated at dosages of 30 and 60 mg·kg^–1·d^–1.The STZ injected mice developed renal function damage,renal inflammation and fibrosis known as diabetic kidney disease(DKD).BPs significantly reduced serum creatinine level and urinary albumin excretion rate,with the attenuated swelling of kidneys.BPs treatment obviously alleviated the pathological damage of renal tissue.The progression of renal injury in BPs treated mice was inhibited with less expression of type IV collagen(Col IV),fibronectin(FN)andα-smooth muscle actin(α-SMA).The inhibition of inflammation in kidney was associated with the reduced level of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).BPs administration suppressed the over-expression of toll like receptor 4(TLR4)and high-mobility group box 1(HMGB1)with lowered activity of nuclear factor kappa B(NF-κB)in renal tissue of diabetic mice.Oral administration of BPs effectively prevented the development of renal injury in diabetic mice.This study suggested that the protection provided by BPs might affect through the interruption of HMGB1-TLR4 pathway,leading to the inhibition of renal inflammation and fibrotic process.
基金supported by the National Natural Science Foundation of China(Nos.30925042,81274165,and 81330089)the State Key Program for New Drugs from the Ministry of Science and Technology,China(No.2012ZX09301001-003)the Science and Technology Commission of Shanghai Municipality(Nos.10XD1405900 and 12JC1400800)
文摘The present study aimed to investigate the effects of polysaccharides extracted from Bupleurum chinense DC(BCPs) on macrophage functions. In the in vivo experiment, 1 m L of 5% sodium thioglycollate was injected into the abdomen of the mice on Day 0 and macrophages were harvested on Day 4. The macrophages were cultured in plates and treated with different concentrations of BCPs and stimulus. Effects of BCPs on macrophage functions were assessed by chemotaxis assay, phagocytosis assay and Enzyme-Linked Immunosorbent Assay(ELISA). Our results showed the enhanced chemotaxis, phagocytosis and secretion of nitric oxide(NO) and inflammatory cytokines by macrophages when treated with BCPs. However, when chemotaxis and phagocytosis were up-regulated by complement components or opsonized particles, BCPs inhibited these effects. Also, the NO production induced by lipopolysaccharides(LPS) was suppressed by BCPs mildly. Moreover, BCPs had an inhibitory effect on the [Ca^(2+)]i elevation of macrophages. These results suggested that BCPs exerted modulatory effects on macrophage functions, which may contribute to developing novel approaches to treating inflammatory diseases.
基金Shanxi Educational Committee(Grant No.20111113)Shanxi Science and Technology Department(Grant No.2016ZD0201)
文摘As a traditional Chinese herbal medicine exhibiting analgesic,fever-reducing and anti-inflammatory effects,Radix Bupleuri（Chai-Hu） is commonly used for the treatment of influenza,which is derived from the dried roots of Bupleurum chinense DC.and Bupleurum scorzonerifolium Willd.Among of diverse chemical components,saikosaponins are the key active components of the herb medicine.In the present study,we established a method of high performance liquid chromatography（HPLC） coupled with evaporative light scattering detection（ELSD） for simultaneous determination of saikosaponin a,c and d in root,stem,leaf and flower of Bupleurum chinense（B chinense） collected from different areas of Shanxi Province,China.The results from 16 samples of root,stem,leaf and flower of B chinense demonstrated that the total contents of the three saikosaponins in the root of B chinense collected from Dongshan Taiyuan,Xishan,Tianlongshan and Pangquangou were 4.26 mg/g,3.22 mg/g,4.23 mg/g and 3.05 mg/g,respectively.However,there was scarcely any saikosaponins in the stem,leaf and flower of B chinense collected from above-mentioned areas.The method of HPLC coupled with ELSD was suitable for quality control of Radix Bupleuri.The result also confirmed that the root of B chinense was the best medicinal part.
