In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germinatio...In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germination temperature and exogenous regulating substances to improve the germination rate. The results showed that the germination rate of fresh water at 12 h was significantly higher than that at 8 h and 36 h; The germination rate of seeds treated with constant temperature 15℃ and variable temperature 25 ℃/15 ℃ （day/night） was significantly higher than that of other temperature treatments. Seed soaking time and culture temperature also influenced seed germination start day and peak day. Different concentrations of regulatory substances could promote seeds germination. The germination rate of seeds induced by 0.50 mg/L 6-BA was the highest, the start day and peak day were 4 days and 5 days earlier than control check （CK） respectively.展开更多
The saikosaponin a, c, d in Chinese Thorowax were determined by RP-HPLC with UV detecter at the wavelength of 205 nm. The optimal experiment conditions were ODS (5 micron) colum, mobile phase of acenitrile-water (4: 6...The saikosaponin a, c, d in Chinese Thorowax were determined by RP-HPLC with UV detecter at the wavelength of 205 nm. The optimal experiment conditions were ODS (5 micron) colum, mobile phase of acenitrile-water (4: 6) and quinoestrol as internal standard. This method is simple, rapid and the reproducibility is good.展开更多
文摘In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germination temperature and exogenous regulating substances to improve the germination rate. The results showed that the germination rate of fresh water at 12 h was significantly higher than that at 8 h and 36 h; The germination rate of seeds treated with constant temperature 15℃ and variable temperature 25 ℃/15 ℃ （day/night） was significantly higher than that of other temperature treatments. Seed soaking time and culture temperature also influenced seed germination start day and peak day. Different concentrations of regulatory substances could promote seeds germination. The germination rate of seeds induced by 0.50 mg/L 6-BA was the highest, the start day and peak day were 4 days and 5 days earlier than control check （CK） respectively.
文摘The saikosaponin a, c, d in Chinese Thorowax were determined by RP-HPLC with UV detecter at the wavelength of 205 nm. The optimal experiment conditions were ODS (5 micron) colum, mobile phase of acenitrile-water (4: 6) and quinoestrol as internal standard. This method is simple, rapid and the reproducibility is good.
