Allii Macrostemonis Bulbus(Xiebai in Chinese), as a famous traditional Chinese medicine, has great medicinal and dietary values since ancient times. In China, the dry bulbs of Allium macrostemon and Allium chinense ar...Allii Macrostemonis Bulbus(Xiebai in Chinese), as a famous traditional Chinese medicine, has great medicinal and dietary values since ancient times. In China, the dry bulbs of Allium macrostemon and Allium chinense are both used as its original plants. Pharmacological studies have revealed that both of them could increase plasminogen activator activity and prolong the effect of coagulation to achieve antiplatelet aggregation which validates their traditional uses for the treatment of thoracic obstruction and cardialgia in clinics. Besides, several other significant activities, including lipid-lowering, anti-atherosclerosis, antitumor, antispasmodic, antibacterial, antioxidant, and insecticidal activities, have already been reported. The volatile oils, nitrogenous compounds, and steroidal saponins are the major beneficial compounds. Among them, steroidal saponins are considered as the characteristic constituents. In this review, the current information concerning the phytochemistry and pharmacology of Allii Macrostemonis Bulbus is summarized comprehensively. In addition, several research future perspectives are presented, especially the mechanism of bioactive components and fraction from the bulbs of Allium macrostemon and Allium chinense.展开更多
Five new spirostanol saponins(1−5)and seven known compounds(6−12)were isolated from the n-butanol fraction of 75%ethanol extract of Allii Macrostemonis Bulbus.The identification and structural elucidation of all the i...Five new spirostanol saponins(1−5)and seven known compounds(6−12)were isolated from the n-butanol fraction of 75%ethanol extract of Allii Macrostemonis Bulbus.The identification and structural elucidation of all the isolates were performed through extensive 1D and 2D NMR experiments,HR-ESI-MS data analysis and comparisons with literature values.Antioxidant evaluation showed that compounds 6−11 exhibited certain scavenging effects on ABTS radical,where compounds 6,7 and 11 had IC50 values of 0.208,0.057 and 0.014 mg·mL−1,respectively.展开更多
Asthma is a chronic respiratory disease characterized by airway inflammation,hyperresponsiveness,and remodeling.The existing therapies have a limited effect on refractory asthma.In this study,Fritillaria Cirrhosae Bul...Asthma is a chronic respiratory disease characterized by airway inflammation,hyperresponsiveness,and remodeling.The existing therapies have a limited effect on refractory asthma.In this study,Fritillaria Cirrhosae Bulbus(BFC),a traditional Chinese medicine,was taken as the object,and the data of network pharmacology,molecular docking,and clinical transcriptomics were integrated to systematically analyze its multi-component synergistic anti-asthma mechanism.By screening 55 active components(mainly alkaloids)of BFC,172 crosstargets related to asthma were predicted,a protein interaction network was constructed,and 20 core targets,such as STAT3,TNF,EGFR,and HSP90AA1/HSP90AB1,were identified.Functional enrichment analysis showed that BFC affected inflammatory response,immune activation,and hormone sensitivity by regulating NF-κB signal,arachidonic acid metabolism,and calcium ion pathway.Molecular docking test showed that alkaloids such as Korseveramine and Peimisine had a high affinity with HSP90,EGFR,and SRC(binding energy<-9 kcal/mol).Based on the differential gene analysis of GSE27876 and GSE74986 datasets,HSP90AA1/HSP90AB1 downregulation in severe asthma was identified,suggesting that BFC may alleviate glucocorticoid resistance by regulating HSP90 function.This study extends the known alkaloid pharmacology of BFC and provides novel mechanistic insights into its complex anti-asthma effects.To our knowledge,this is the first study integrating network pharmacology,molecular docking,and clinical transcriptomics to systematically characterize the antiasthma mechanism of BFC.展开更多
基金financially supported by the Macao Science and Technology Development Fund(No.035/2014/A1)
文摘Allii Macrostemonis Bulbus(Xiebai in Chinese), as a famous traditional Chinese medicine, has great medicinal and dietary values since ancient times. In China, the dry bulbs of Allium macrostemon and Allium chinense are both used as its original plants. Pharmacological studies have revealed that both of them could increase plasminogen activator activity and prolong the effect of coagulation to achieve antiplatelet aggregation which validates their traditional uses for the treatment of thoracic obstruction and cardialgia in clinics. Besides, several other significant activities, including lipid-lowering, anti-atherosclerosis, antitumor, antispasmodic, antibacterial, antioxidant, and insecticidal activities, have already been reported. The volatile oils, nitrogenous compounds, and steroidal saponins are the major beneficial compounds. Among them, steroidal saponins are considered as the characteristic constituents. In this review, the current information concerning the phytochemistry and pharmacology of Allii Macrostemonis Bulbus is summarized comprehensively. In addition, several research future perspectives are presented, especially the mechanism of bioactive components and fraction from the bulbs of Allium macrostemon and Allium chinense.
基金Jilin Science and Technology Development Program(No.20200404023YY).
文摘Five new spirostanol saponins(1−5)and seven known compounds(6−12)were isolated from the n-butanol fraction of 75%ethanol extract of Allii Macrostemonis Bulbus.The identification and structural elucidation of all the isolates were performed through extensive 1D and 2D NMR experiments,HR-ESI-MS data analysis and comparisons with literature values.Antioxidant evaluation showed that compounds 6−11 exhibited certain scavenging effects on ABTS radical,where compounds 6,7 and 11 had IC50 values of 0.208,0.057 and 0.014 mg·mL−1,respectively.
基金supported by the Project of National key R&D plan(No.:2023YFD1600400)Shaanxi Traditional Chinese Medicine Administration,China(No.2019-13).
文摘Asthma is a chronic respiratory disease characterized by airway inflammation,hyperresponsiveness,and remodeling.The existing therapies have a limited effect on refractory asthma.In this study,Fritillaria Cirrhosae Bulbus(BFC),a traditional Chinese medicine,was taken as the object,and the data of network pharmacology,molecular docking,and clinical transcriptomics were integrated to systematically analyze its multi-component synergistic anti-asthma mechanism.By screening 55 active components(mainly alkaloids)of BFC,172 crosstargets related to asthma were predicted,a protein interaction network was constructed,and 20 core targets,such as STAT3,TNF,EGFR,and HSP90AA1/HSP90AB1,were identified.Functional enrichment analysis showed that BFC affected inflammatory response,immune activation,and hormone sensitivity by regulating NF-κB signal,arachidonic acid metabolism,and calcium ion pathway.Molecular docking test showed that alkaloids such as Korseveramine and Peimisine had a high affinity with HSP90,EGFR,and SRC(binding energy<-9 kcal/mol).Based on the differential gene analysis of GSE27876 and GSE74986 datasets,HSP90AA1/HSP90AB1 downregulation in severe asthma was identified,suggesting that BFC may alleviate glucocorticoid resistance by regulating HSP90 function.This study extends the known alkaloid pharmacology of BFC and provides novel mechanistic insights into its complex anti-asthma effects.To our knowledge,this is the first study integrating network pharmacology,molecular docking,and clinical transcriptomics to systematically characterize the antiasthma mechanism of BFC.
