Hazelnut husk brown rot has been identified as a new disease in Liaoning Province in recent years.The objective of this study as to identify the pathogen.[Method]In this study,a standard sample of hazelnut husk brown ...Hazelnut husk brown rot has been identified as a new disease in Liaoning Province in recent years.The objective of this study as to identify the pathogen.[Method]In this study,a standard sample of hazelnut husk brown rot was collected from Songmudao Base in Dalian City,Liaoning Province.The pathogen was identified by the studies of the morphology,pathogenicity,and analyses of ITS and LSU sequences.The pathogen was isolated and purified,which was confirmed by Koch’s postulates.The symptoms after inoculation were the same as those collected directly from a diseased tree,which showed that it was the pathogenic fungus.The cultural characteristics and conidia and the morphology of the pathogenic fungi were similar to those of Botrytis cinerea’s.The ITS sequences and LSU sequences were compared to the associated strain sequences in GenBank,with 100%identity to Botrytis cinerea(GenBank accession number:MN589848.1)and Botrytis cinerea(GenBank accession number:KU140653.1),respectively.The infection status of the pathogen on the hazelnut husks was also observed.The studies suggested that the pathogen leading to the hazelnut husk brown rot as a new disease in Liaoning Province was Botrytis cinerea.展开更多
Coniophora is a widely distributed genus that includes species with resupinate basidiomata causing brown rot on fallen trees.In this study,two new species,Coniophora granulosa and Coniophora hainanensis,are described ...Coniophora is a widely distributed genus that includes species with resupinate basidiomata causing brown rot on fallen trees.In this study,two new species,Coniophora granulosa and Coniophora hainanensis,are described from Hainan Province based on molecular and morphological evidence.C.granulosa is characterized by having coriaceous basidiomata with granulose and light orange to grayish-orange hymenophore,relatively large and ellipsoid basidiospores measuring 12.5–14.2×8.2–9.5μm.C.hainanensis is characterized by having membranaceous basidiomata with smooth and grayish-orange to brownish orange hymenophore and fimbriate margin,and oblong-ellipsoid basidiospores measuring 9.8–12.2×5.2–6.5μm.Descriptions and illustrations are provided for the two new species.展开更多
探究柑橘疫霉褐腐病病原菌生长特性及有效药剂筛选,可对防控柑橘疫霉褐腐病(citrus brown rot)的发生及防治提供参考。采用菌丝生长速率法测定柑橘褐腐疫霉(WZYM1菌株,保存于重庆三峡农业科学院)在不同培养基、温度、pH值、光照、碳源...探究柑橘疫霉褐腐病病原菌生长特性及有效药剂筛选,可对防控柑橘疫霉褐腐病(citrus brown rot)的发生及防治提供参考。采用菌丝生长速率法测定柑橘褐腐疫霉(WZYM1菌株,保存于重庆三峡农业科学院)在不同培养基、温度、pH值、光照、碳源、氮源等条件下的生长特性,并选取24种市面常见的杀菌剂进行毒力测定。结果表明,柑橘褐腐疫霉在PDA培养基下生长较好,培养5 d后菌落直径达43.49 mm;在22~30℃温度环境下生长状态最佳,且在25℃时生长最快,培养5 d后菌落直径达42.54 mm;在pH值为6~8时菌落生长较快;在碳源上,对可溶性淀粉的利用效果较好,而对麦芽糖、D-果糖利用效果较差;在氮源上,对蛋白胨和酵母膏利用效果较好,而对尿素及碳酸铵利用效果较差。供试24种杀菌剂均对柑橘褐腐疫霉有抑制效果,春雷·喹啉铜、喹啉铜和吡唑醚菌酯具有较强的毒力,EC_(50)值分别为29.423、35.674和40.962 mg/L。柑橘褐腐疫霉最适在PDA培养基、温度为22~30℃、pH值为6~8、可溶性淀粉碳源、蛋白胨和酵母膏氮源环境下生长。30%吡唑醚菌酯悬浮剂、33.5%喹啉铜悬浮剂和33%春雷·喹啉铜悬浮剂对柑橘褐腐疫霉的抑制效果较好,可作为柑橘疫霉褐腐病田间防治参考用药。展开更多
基金This work was financially supported by the Liaoning Provincial Natural Science Foundation of China(Grant No.2021-MS-057).
文摘Hazelnut husk brown rot has been identified as a new disease in Liaoning Province in recent years.The objective of this study as to identify the pathogen.[Method]In this study,a standard sample of hazelnut husk brown rot was collected from Songmudao Base in Dalian City,Liaoning Province.The pathogen was identified by the studies of the morphology,pathogenicity,and analyses of ITS and LSU sequences.The pathogen was isolated and purified,which was confirmed by Koch’s postulates.The symptoms after inoculation were the same as those collected directly from a diseased tree,which showed that it was the pathogenic fungus.The cultural characteristics and conidia and the morphology of the pathogenic fungi were similar to those of Botrytis cinerea’s.The ITS sequences and LSU sequences were compared to the associated strain sequences in GenBank,with 100%identity to Botrytis cinerea(GenBank accession number:MN589848.1)and Botrytis cinerea(GenBank accession number:KU140653.1),respectively.The infection status of the pathogen on the hazelnut husks was also observed.The studies suggested that the pathogen leading to the hazelnut husk brown rot as a new disease in Liaoning Province was Botrytis cinerea.
基金supported by the National Natural Science Foundation of China(32270014)the Fundamental Research Funds for the Central Universities(QNTD202307).
文摘Coniophora is a widely distributed genus that includes species with resupinate basidiomata causing brown rot on fallen trees.In this study,two new species,Coniophora granulosa and Coniophora hainanensis,are described from Hainan Province based on molecular and morphological evidence.C.granulosa is characterized by having coriaceous basidiomata with granulose and light orange to grayish-orange hymenophore,relatively large and ellipsoid basidiospores measuring 12.5–14.2×8.2–9.5μm.C.hainanensis is characterized by having membranaceous basidiomata with smooth and grayish-orange to brownish orange hymenophore and fimbriate margin,and oblong-ellipsoid basidiospores measuring 9.8–12.2×5.2–6.5μm.Descriptions and illustrations are provided for the two new species.
