AIM： To compare the efficacy, tolerability and safety of bromfenac 0.09%, nepafenac 0.1% or diclofenac 0.1% for the prophylaxis of the cystoid macular edema（CME） after phacoemulsification. METHODS： Group sequentia...AIM： To compare the efficacy, tolerability and safety of bromfenac 0.09%, nepafenac 0.1% or diclofenac 0.1% for the prophylaxis of the cystoid macular edema（CME） after phacoemulsification. METHODS： Group sequential observational comparative study. After phacoemulsification, patients received two months for topical treatment of either diclofenac sodium, bromfenac or nepafenac. All patients received concomitant topical tobramycin 0.3% and topical prednisolone 1%. We measured CME using optical coherence tomography（OCT） central foveal thickness, macular thickness and total macular volume. RESULTS： We enrolled 243 patients from January to June 2015, and 35% received diclofenac, 32.9% bromfenac and 32.1% nepafenac. When we compared pre-operative to three weeks to two months, bromfenac was more effective in reducing foveal volume（21.3 and 35.4 mm3, respectively）, compared with the diclofenac（1.3 and 11.5 mm3, respectively）, and the nepafenac group, became more edematous 6.4 and 5.3, respectively. Totally 133 patients completed the post-surgical satisfaction questionnaire. Patients complained of eye stickiness in 13.8% whom we gave nepafenac, versus 10.3% whom we gave diclofenac sodium, and in 0 whom we gave bromfenac. CONCLUSION： Bromfenac is the best tolerated and is more effective than diclofenac and nepafenac in reducing CME after phacoemulsification.展开更多
This work is a contribution of theoretical chemistry to the classification of some non-steroidal anti-inflammatory drugs (NSAIDs). Indeed, research on the efficacy of NSAIDs has shown that no NSAID is recognized as th...This work is a contribution of theoretical chemistry to the classification of some non-steroidal anti-inflammatory drugs (NSAIDs). Indeed, research on the efficacy of NSAIDs has shown that no NSAID is recognized as the most efficient anti-inflammatory drug. We have made a theoretical study of diclofenac, bromfenac and amfenac, in order to compare their efficacy from some physicochemical properties. To do this, we used the DFT and TD-DTF methods at the B3LYP/6-311+G(d, p) level theory. The lipophilicity study shows that diclofenac and bromfenac are very lipophilic. Acidity study shows that diclofenac is more acid than bromfenac and amfenac. The results from molecular orbital and the TD-DFT calculations reveal that for the three NSAIDs, the lowest energy transition is due to the excitation from HOMO to LUMO. The absorption energy corresponding to H→L transition is comparable with the energy gap value. Our findings have shown that bromfenac is more reactive than amfenac, which is more reactive than diclofenac.展开更多
Cataract is the leading cause of visual impairment,and posterior capsular opacification(PCO)is the most common long-term complication of modern cataract surgery,which can cause severe visual impairment after surgery.T...Cataract is the leading cause of visual impairment,and posterior capsular opacification(PCO)is the most common long-term complication of modern cataract surgery,which can cause severe visual impairment after surgery.The proliferation,migration,and epithelial-mesenchymal transition(EMT)of residual lens epithelial cells(LECs)stimulated by growth factors and cytokines,are the key pathological mechanisms involved in the development of PCO.This study demonstrated that non-steroidal anti-inflammatory drug(NSAID),bromfenac,was capable of effectively inhibiting cell migration,overexpression of EMT markers,such as fibronectin(FN),matrix metalloproteinase 2(MMP2),α-smooth muscle actin(α-SMA),and transcription factor Snail,and extracellular signal-regulated kinase(ERK)/glycogen synthase kinase-3β(GSK-3β)signaling induced by transforming growth factor-β2(TGF-β2)in vitro.The inhibitory effect of bromfenac on TGF-β2-induced EMT was also verified on a primary lens epithelial cell model using human anterior capsules.Furthermore,based on ultrasonic spray technology,we developed a drug-eluting intraocular lens(IOL)using poly(lactic-co-glycolic acid)(PLGA)with sustained bromfenac release ability for the prevention of PCO development.In the rabbit models of cataract surgery,bromfenac-eluting IOL exhibited remarkable PCO prevention and inflammation suppression effects with excellent biocompatibility.In conclusion,bromfenac can inhibit TGF-β2-induced cell migration and the EMT of LECs via ERK/GSK-3β/Snail signaling.The present study offers a novel approach for preventing PCO through PLGA-based drug sustained-release IOLs.展开更多
目的制备一种具有双重缓释功能的新型生物复合材料溴芬酸钠/壳聚糖纳米粒胶联羊膜(BF/CS-FBAM),初步研究其体外缓释性能及对角膜新生血管的影响。方法采用离子胶联法制备溴芬酸钠/壳聚糖纳米粒并观察其表征,将载药纳米粒混入纤维蛋白胶...目的制备一种具有双重缓释功能的新型生物复合材料溴芬酸钠/壳聚糖纳米粒胶联羊膜(BF/CS-FBAM),初步研究其体外缓释性能及对角膜新生血管的影响。方法采用离子胶联法制备溴芬酸钠/壳聚糖纳米粒并观察其表征,将载药纳米粒混入纤维蛋白胶中,再将混合物喷涂到羊膜表面制成BF/CS-FBAM,并进行形态学和体外释药观察,最后通过兔角膜碱烧伤模型观察BF/CS-FBAM对角膜新生血管的影响,用实时荧光定量PCR方法检测术后角膜中VEGF的表达。结果溴芬酸钠/壳聚糖纳米粒呈球形,表面光滑,大小均一,其粒径为(548.7±14.4)nm,Zeta电位为(29.8±2.2)mV,包封率为(67.11±4.56)%,载药量为(57.71±5.38)%。载有纳米药的纤维蛋白胶与羊膜黏合紧密,表面呈网状结构,纳米药充斥其中。在体外,BF/CS-FBAM的释药可达14 d。在碱烧伤模型中,术后BF/CS-FBAM组和BF/CS组在减少炎症反应、抑制新生血管及降低VEGF表达方面都显著优于对照组,且各时间点的差异有统计学意义(P<0.05);同时,术后14 d和21 d BF/CS-FBAM组中VEGF的表达明显低于BF/CS组(P<0.05)。结论制备的BF/CS-FBAM作为一种新型生物移植材料可在局部缓慢释放溴芬酸钠,并对角膜新生血管的产生有较好的抑制性。展开更多
文摘AIM： To compare the efficacy, tolerability and safety of bromfenac 0.09%, nepafenac 0.1% or diclofenac 0.1% for the prophylaxis of the cystoid macular edema（CME） after phacoemulsification. METHODS： Group sequential observational comparative study. After phacoemulsification, patients received two months for topical treatment of either diclofenac sodium, bromfenac or nepafenac. All patients received concomitant topical tobramycin 0.3% and topical prednisolone 1%. We measured CME using optical coherence tomography（OCT） central foveal thickness, macular thickness and total macular volume. RESULTS： We enrolled 243 patients from January to June 2015, and 35% received diclofenac, 32.9% bromfenac and 32.1% nepafenac. When we compared pre-operative to three weeks to two months, bromfenac was more effective in reducing foveal volume（21.3 and 35.4 mm3, respectively）, compared with the diclofenac（1.3 and 11.5 mm3, respectively）, and the nepafenac group, became more edematous 6.4 and 5.3, respectively. Totally 133 patients completed the post-surgical satisfaction questionnaire. Patients complained of eye stickiness in 13.8% whom we gave nepafenac, versus 10.3% whom we gave diclofenac sodium, and in 0 whom we gave bromfenac. CONCLUSION： Bromfenac is the best tolerated and is more effective than diclofenac and nepafenac in reducing CME after phacoemulsification.
文摘This work is a contribution of theoretical chemistry to the classification of some non-steroidal anti-inflammatory drugs (NSAIDs). Indeed, research on the efficacy of NSAIDs has shown that no NSAID is recognized as the most efficient anti-inflammatory drug. We have made a theoretical study of diclofenac, bromfenac and amfenac, in order to compare their efficacy from some physicochemical properties. To do this, we used the DFT and TD-DTF methods at the B3LYP/6-311+G(d, p) level theory. The lipophilicity study shows that diclofenac and bromfenac are very lipophilic. Acidity study shows that diclofenac is more acid than bromfenac and amfenac. The results from molecular orbital and the TD-DFT calculations reveal that for the three NSAIDs, the lowest energy transition is due to the excitation from HOMO to LUMO. The absorption energy corresponding to H→L transition is comparable with the energy gap value. Our findings have shown that bromfenac is more reactive than amfenac, which is more reactive than diclofenac.
基金This study was supported by the National Natural Science Foundation of China(Grant Nos.82070939,22005265,81870641,and 81800809)the National Key R&D Program of China(No.2018YFC1106104)+1 种基金Key Research and Development Project of Zhejiang Province of China(No.2020C03035)and the Joint Funds of the Zhejiang Provincial Natural Science Foundation of China(Grant No.LBY21E030002).
文摘Cataract is the leading cause of visual impairment,and posterior capsular opacification(PCO)is the most common long-term complication of modern cataract surgery,which can cause severe visual impairment after surgery.The proliferation,migration,and epithelial-mesenchymal transition(EMT)of residual lens epithelial cells(LECs)stimulated by growth factors and cytokines,are the key pathological mechanisms involved in the development of PCO.This study demonstrated that non-steroidal anti-inflammatory drug(NSAID),bromfenac,was capable of effectively inhibiting cell migration,overexpression of EMT markers,such as fibronectin(FN),matrix metalloproteinase 2(MMP2),α-smooth muscle actin(α-SMA),and transcription factor Snail,and extracellular signal-regulated kinase(ERK)/glycogen synthase kinase-3β(GSK-3β)signaling induced by transforming growth factor-β2(TGF-β2)in vitro.The inhibitory effect of bromfenac on TGF-β2-induced EMT was also verified on a primary lens epithelial cell model using human anterior capsules.Furthermore,based on ultrasonic spray technology,we developed a drug-eluting intraocular lens(IOL)using poly(lactic-co-glycolic acid)(PLGA)with sustained bromfenac release ability for the prevention of PCO development.In the rabbit models of cataract surgery,bromfenac-eluting IOL exhibited remarkable PCO prevention and inflammation suppression effects with excellent biocompatibility.In conclusion,bromfenac can inhibit TGF-β2-induced cell migration and the EMT of LECs via ERK/GSK-3β/Snail signaling.The present study offers a novel approach for preventing PCO through PLGA-based drug sustained-release IOLs.
文摘目的制备一种具有双重缓释功能的新型生物复合材料溴芬酸钠/壳聚糖纳米粒胶联羊膜(BF/CS-FBAM),初步研究其体外缓释性能及对角膜新生血管的影响。方法采用离子胶联法制备溴芬酸钠/壳聚糖纳米粒并观察其表征,将载药纳米粒混入纤维蛋白胶中,再将混合物喷涂到羊膜表面制成BF/CS-FBAM,并进行形态学和体外释药观察,最后通过兔角膜碱烧伤模型观察BF/CS-FBAM对角膜新生血管的影响,用实时荧光定量PCR方法检测术后角膜中VEGF的表达。结果溴芬酸钠/壳聚糖纳米粒呈球形,表面光滑,大小均一,其粒径为(548.7±14.4)nm,Zeta电位为(29.8±2.2)mV,包封率为(67.11±4.56)%,载药量为(57.71±5.38)%。载有纳米药的纤维蛋白胶与羊膜黏合紧密,表面呈网状结构,纳米药充斥其中。在体外,BF/CS-FBAM的释药可达14 d。在碱烧伤模型中,术后BF/CS-FBAM组和BF/CS组在减少炎症反应、抑制新生血管及降低VEGF表达方面都显著优于对照组,且各时间点的差异有统计学意义(P<0.05);同时,术后14 d和21 d BF/CS-FBAM组中VEGF的表达明显低于BF/CS组(P<0.05)。结论制备的BF/CS-FBAM作为一种新型生物移植材料可在局部缓慢释放溴芬酸钠,并对角膜新生血管的产生有较好的抑制性。
