The diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera: Plutellidae) is an insect pest found around the world that feeds on cruciferous crops. The DBM has become resistant to most insecticides in current use...The diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera: Plutellidae) is an insect pest found around the world that feeds on cruciferous crops. The DBM has become resistant to most insecticides in current use in the field. Broflanilide is a novel meta-diamide insecticide that binds to a new site on the γ-aminobutyric acid receptor and very efficiently protects against most pests in the order Lepidoptera, including DBM. In this study, the resistance of a laboratory-bred susceptible strain of DBM to broflanilide and the fitness costs posed by broflanilide to the DBM were evaluated. The DBM had no obvious resistance to broflanilide after 10 generations of selection. The realized heritability h2 was 0.033, suggesting a low risk of resistance developing in this strain. The F10 generation had no cross-resistance to the insecticides abamectin and endosulfan (which target the γ-aminobutyric acid receptor) and chlorantraniliprole (which targets a non-γ-aminobutyric acid receptor). The specific activities of important detoxification enzymes (cytochrome P450 monooxygenase, esterase, and glutathione S-transferase) were not obviously altered. However, the larval stage was prolonged and the adult stage was shortened significantly in F11 generation than the F0 generation. The total preoviposition period TPOP significantly prolonged 1.90 d in F11 generation. The fitness value Rf (0.93) was lower for the F11 generation than the F0 generation. The results indicated that long-term exposure to broflanilide exerts clear fitness costs in the DBM. This information will be useful in identifying reasonable broflanilide application guidelines for managing broflanilide resistance in the DBM.展开更多
Broflanilide is a novel meta-diamide insecticide that acts as a y-aminobutyric acid-gated chloride channel allosteric modulator.With its unique mode of action,broflanilide has no known cross-resistance with existing i...Broflanilide is a novel meta-diamide insecticide that acts as a y-aminobutyric acid-gated chloride channel allosteric modulator.With its unique mode of action,broflanilide has no known cross-resistance with existing insecticides and is expected to be an effective tool for the management of insecticide resistance.Establishing the base-line susceptibility to this insecticide is an essential step for developing and implementing effective resistance management strategies.Here we evaluated the baseline susceptibil-ity to broflanilide for 3 cosmopolitan lepidopteran pest species,Helicoverpa armigera,Plutella xylostella,and Spodoptera frugiperda.Broflanilide exhibited high activity against populations sampled in the major distribution range of these pests in China,with median lethal concentrations(LCso)ranging between 0.209 and 0.684,0.076 and 0.336,and 0.075 and 0.219 mg/L for H.armigera,P xylostella,and S.frugiperda,respectively.Among-population variability in susceptibility to broflanilide was moderate for H.armigera(3.3-fold),P xylostella(4.4-fold),and S.frugiperda(2.9-fold).The recommended diagnostic concentrations for H.armigera,P xylostella,and S.frugiperda were 8,4,and 2 mg/L,re-spectively.Little or no cross-resistance to broflanilide was detected in 3 diamide-resistant strains of P xylostella and 1 spinosyns-resistant strain of S.frugiperda.Our results provide critical information for the development of effective resistance management programs to sustain efficacy of broflanilide against these key lepidopteran pests.展开更多
基金This work was supported by the National Natural Science Foundation of China(31371956,31572023 and 31772186).
文摘The diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera: Plutellidae) is an insect pest found around the world that feeds on cruciferous crops. The DBM has become resistant to most insecticides in current use in the field. Broflanilide is a novel meta-diamide insecticide that binds to a new site on the γ-aminobutyric acid receptor and very efficiently protects against most pests in the order Lepidoptera, including DBM. In this study, the resistance of a laboratory-bred susceptible strain of DBM to broflanilide and the fitness costs posed by broflanilide to the DBM were evaluated. The DBM had no obvious resistance to broflanilide after 10 generations of selection. The realized heritability h2 was 0.033, suggesting a low risk of resistance developing in this strain. The F10 generation had no cross-resistance to the insecticides abamectin and endosulfan (which target the γ-aminobutyric acid receptor) and chlorantraniliprole (which targets a non-γ-aminobutyric acid receptor). The specific activities of important detoxification enzymes (cytochrome P450 monooxygenase, esterase, and glutathione S-transferase) were not obviously altered. However, the larval stage was prolonged and the adult stage was shortened significantly in F11 generation than the F0 generation. The total preoviposition period TPOP significantly prolonged 1.90 d in F11 generation. The fitness value Rf (0.93) was lower for the F11 generation than the F0 generation. The results indicated that long-term exposure to broflanilide exerts clear fitness costs in the DBM. This information will be useful in identifying reasonable broflanilide application guidelines for managing broflanilide resistance in the DBM.
基金supported by grants from National Key Research and Development Program of China(2021YFD1400704)the Sanya Institute of Nanjing Agricultural University(No.NAUSY-MS09).
文摘Broflanilide is a novel meta-diamide insecticide that acts as a y-aminobutyric acid-gated chloride channel allosteric modulator.With its unique mode of action,broflanilide has no known cross-resistance with existing insecticides and is expected to be an effective tool for the management of insecticide resistance.Establishing the base-line susceptibility to this insecticide is an essential step for developing and implementing effective resistance management strategies.Here we evaluated the baseline susceptibil-ity to broflanilide for 3 cosmopolitan lepidopteran pest species,Helicoverpa armigera,Plutella xylostella,and Spodoptera frugiperda.Broflanilide exhibited high activity against populations sampled in the major distribution range of these pests in China,with median lethal concentrations(LCso)ranging between 0.209 and 0.684,0.076 and 0.336,and 0.075 and 0.219 mg/L for H.armigera,P xylostella,and S.frugiperda,respectively.Among-population variability in susceptibility to broflanilide was moderate for H.armigera(3.3-fold),P xylostella(4.4-fold),and S.frugiperda(2.9-fold).The recommended diagnostic concentrations for H.armigera,P xylostella,and S.frugiperda were 8,4,and 2 mg/L,re-spectively.Little or no cross-resistance to broflanilide was detected in 3 diamide-resistant strains of P xylostella and 1 spinosyns-resistant strain of S.frugiperda.Our results provide critical information for the development of effective resistance management programs to sustain efficacy of broflanilide against these key lepidopteran pests.
