Fucose-containing oligosaccharides exhibit various biological activities,particularly for improving the intestinal health of infants.Bacterial exopolysaccharides are promising resource of fucose-containing oligosaccha...Fucose-containing oligosaccharides exhibit various biological activities,particularly for improving the intestinal health of infants.Bacterial exopolysaccharides are promising resource of fucose-containing oligosaccharides.A fucose-containing trisaccharide(_(D-)Glcp-β-(1→4)_(-L)-Fucp and _(D-)Galp-α-(1→3)_(-L)-Fucp,GuFGa)with same monosaccharide composition with 2′-fucosyllactose(2′-FL,the most abundant oligosaccharide in human milk),is obtained from bacterial exopolysaccharides.This study compared the in vitro prebiotic activities of GuFGa and 2′-FL using mono-and co-culture fermentation.Mono-culture shows GuFGa could be utilized by Bifidobacterium infantis and Lactobacillus plantarum,while 2′-FL only promote the proliferation of B.infantis.Co-culture fermentation involves cross-feeding between B.infantis and L.plantarum to utilize GuFGa.Further in vitro fecal microbiota fermentation of breastfed infants demonstrates that GuFGa exhibits better prebiotic activity than 2′-FL in increasing beneficial bacteria(Bifidobacterium and Lactobacillus)abundance and reducing harmful bacteria(Clostridium_sensu_stricto and Escherichia-Shigella)abundance.Moreover,GuFGa and 2′-FL fermentation shifted the gut microbiota composition of formula-fed infants to that of breastfed infants.Furthermore,herein,GuFGa and 2′-FL caused distinct metabolic profiles in both short-chain fatty acids and indole derivatives(e.g.indolelactic acid and indolepropionic acid).In summary,this study provides insights into the exploration of novel functional fucose-containing oligosaccharides as potential prebiotics.展开更多
基金supported by the National Natural Science Foundation of China(31872893 and 32202064)China Postdoctoral Science Foundation(2021M701547)+1 种基金Fundamental Research Funds for the Central Universities(202261043)Natural Science Foundation of Jiangxi Province(20224BAB215044).
文摘Fucose-containing oligosaccharides exhibit various biological activities,particularly for improving the intestinal health of infants.Bacterial exopolysaccharides are promising resource of fucose-containing oligosaccharides.A fucose-containing trisaccharide(_(D-)Glcp-β-(1→4)_(-L)-Fucp and _(D-)Galp-α-(1→3)_(-L)-Fucp,GuFGa)with same monosaccharide composition with 2′-fucosyllactose(2′-FL,the most abundant oligosaccharide in human milk),is obtained from bacterial exopolysaccharides.This study compared the in vitro prebiotic activities of GuFGa and 2′-FL using mono-and co-culture fermentation.Mono-culture shows GuFGa could be utilized by Bifidobacterium infantis and Lactobacillus plantarum,while 2′-FL only promote the proliferation of B.infantis.Co-culture fermentation involves cross-feeding between B.infantis and L.plantarum to utilize GuFGa.Further in vitro fecal microbiota fermentation of breastfed infants demonstrates that GuFGa exhibits better prebiotic activity than 2′-FL in increasing beneficial bacteria(Bifidobacterium and Lactobacillus)abundance and reducing harmful bacteria(Clostridium_sensu_stricto and Escherichia-Shigella)abundance.Moreover,GuFGa and 2′-FL fermentation shifted the gut microbiota composition of formula-fed infants to that of breastfed infants.Furthermore,herein,GuFGa and 2′-FL caused distinct metabolic profiles in both short-chain fatty acids and indole derivatives(e.g.indolelactic acid and indolepropionic acid).In summary,this study provides insights into the exploration of novel functional fucose-containing oligosaccharides as potential prebiotics.
