Objective:To investigate the relationship between the temperature and the microvascular blood flow of the cerebral cortex, and the influence of electro-acupuncture (EA) on the cortical microcirculation. Methods: High ...Objective:To investigate the relationship between the temperature and the microvascular blood flow of the cerebral cortex, and the influence of electro-acupuncture (EA) on the cortical microcirculation. Methods: High temperature spots on the anterior ectosylvian and low temperature spots on the posterior suprasylvian on the cortical surface of 20 cats were identified using cortical infrared thermography (CIT); the blood flow in the microcirculation on these spots was measured with laser-Doppler flowmetry. EA was given at Zusanli (ST 36) and changes in the blood flow in the cerebral cortex microcirculation were detected. Results: 1) The mean temperatures on the high (34.83±0.24°C) and low (32.28±0.27°C) temperature spots were significantly different (P<0.001); this was indicative of a temperature difference on the cortical surface; 2) The average blood flow in the microcirculation of the high (266.8±19.2 PU) and low (140.8±9.9 PU) temperature spots was significantly different (P<0.001). 3) On the cortical high temperature spots, the mean blood flow in the microcirculation significantly increased from 266.8±86.8 PU before EA, to 422.5±47.4 PU following 5 minutes of EA (58.35%; P<0.01), and 431.8±52.8 PU 5 minutes after ceasing EA (61.84%; P<0.01). 4) On the low temperature spots, there were no significant differences in blood flow following 5 minutes of EA (146.3±11.5 PU), and 5 minutes after ceasing EA (140.5±11.6 PU), when compared with that before acupuncture (140.8±9.9 PU; P>0.9). Conclusion: The high temperature spots of the cortex are active functional regions of neurons with higher blood flow and a stronger response to EA. EA induces a significant increase in blood flow in the high temperature spots of the cortex.展开更多
Brain temperature in different regions plays a crucial role in physiological and pathological processes.However,current techniques face challenges in achieving high spatiotemporal resolution and antifouling properties...Brain temperature in different regions plays a crucial role in physiological and pathological processes.However,current techniques face challenges in achieving high spatiotemporal resolution and antifouling properties for long-term in vivo applications.Herein,we develop an implantable iontronic thermometer based on a hydrogel-filled micropipette for real-time and in situ monitoring of brain temperature fluctuations with high spatiotemporal resolution.The hydrogel-filled micropipette was fabricated with highly hydrophilic and narrow-mesh hydrogel with excellent antifouling properties against various proteins.The fabricated micro-thermometer exhibited a sensitivity of 2.81 nA/℃and a detection limit of 0.15℃.In vivo experiments validated the ability of the micro-thermometer to accurately monitor dynamic temperature changes in rat brains during hyperthermia.Owing to the promising performance of the micro-thermometer,we have,for the first timeuncovered dynamic temperature changes during acute fever.The study paves the way for advancements in brain temperature-related physiological and pathological research.展开更多
基金supported by a grant from the National Natural Science Fund of China (No. 39770925)Science Research Fund of The Ministry of Personnel for Excellent Returnees (1998)Beijing Key Laboratory Fund of The Science and Technology Commission of Beijing (951890600)
文摘Objective:To investigate the relationship between the temperature and the microvascular blood flow of the cerebral cortex, and the influence of electro-acupuncture (EA) on the cortical microcirculation. Methods: High temperature spots on the anterior ectosylvian and low temperature spots on the posterior suprasylvian on the cortical surface of 20 cats were identified using cortical infrared thermography (CIT); the blood flow in the microcirculation on these spots was measured with laser-Doppler flowmetry. EA was given at Zusanli (ST 36) and changes in the blood flow in the cerebral cortex microcirculation were detected. Results: 1) The mean temperatures on the high (34.83±0.24°C) and low (32.28±0.27°C) temperature spots were significantly different (P<0.001); this was indicative of a temperature difference on the cortical surface; 2) The average blood flow in the microcirculation of the high (266.8±19.2 PU) and low (140.8±9.9 PU) temperature spots was significantly different (P<0.001). 3) On the cortical high temperature spots, the mean blood flow in the microcirculation significantly increased from 266.8±86.8 PU before EA, to 422.5±47.4 PU following 5 minutes of EA (58.35%; P<0.01), and 431.8±52.8 PU 5 minutes after ceasing EA (61.84%; P<0.01). 4) On the low temperature spots, there were no significant differences in blood flow following 5 minutes of EA (146.3±11.5 PU), and 5 minutes after ceasing EA (140.5±11.6 PU), when compared with that before acupuncture (140.8±9.9 PU; P>0.9). Conclusion: The high temperature spots of the cortex are active functional regions of neurons with higher blood flow and a stronger response to EA. EA induces a significant increase in blood flow in the high temperature spots of the cortex.
基金the financial support from the National Natural Science Foundation of China(grant nos.22125406,22074149 for P.Y.,22134002 for L.M.,and 22304011 for C.P.)the Natural Science Foundation of Beijing(grant nos.F251009 and Z230022 for P.Y.,2242028 for W.M.,)the National Basic Research Program of China(grant nos.2022YFA1204500 and 2022YFA1204503 for P.Y.,2024YFA1803403 for L.M.).
文摘Brain temperature in different regions plays a crucial role in physiological and pathological processes.However,current techniques face challenges in achieving high spatiotemporal resolution and antifouling properties for long-term in vivo applications.Herein,we develop an implantable iontronic thermometer based on a hydrogel-filled micropipette for real-time and in situ monitoring of brain temperature fluctuations with high spatiotemporal resolution.The hydrogel-filled micropipette was fabricated with highly hydrophilic and narrow-mesh hydrogel with excellent antifouling properties against various proteins.The fabricated micro-thermometer exhibited a sensitivity of 2.81 nA/℃and a detection limit of 0.15℃.In vivo experiments validated the ability of the micro-thermometer to accurately monitor dynamic temperature changes in rat brains during hyperthermia.Owing to the promising performance of the micro-thermometer,we have,for the first timeuncovered dynamic temperature changes during acute fever.The study paves the way for advancements in brain temperature-related physiological and pathological research.
