Clock genes are involved in circadian rhythm regulation, and surviving newborns with hypoxic-ischemic encephalopathy may present with sleep-wake cycle reversal. This study aimed to determine the expression of the cloc...Clock genes are involved in circadian rhythm regulation, and surviving newborns with hypoxic-ischemic encephalopathy may present with sleep-wake cycle reversal. This study aimed to determine the expression of the clock genes Clock and Bmall, in the pineal gland of rats with hypoxic-ischemic brain damage. Results showed that levels of Clock mRNA v^re not significantly changed within 48 hours after cerebral hypoxia and ischemia. Expression levels of CLOCK and BMAL1 protein were significantly higher after 48 hours. The levels of Bmall mRNA reached a peak at 36 hours, but were significantly reduced at 48 hours. Experimental findings indicate that Clock and Bmall genes were indeed expressed in the pineal glands of neonatal rats. At the initial stage （within 36 hours） of hypoxic-ischemic brain damage, only slight changes in the expression levels of these two genes were detected, followed by significant changes at 36-48 hours. These changes may be associated with circadian rhythm disorder induced by hypoxic-ischemic brain damage.展开更多
目的:通过比较足月缺氧缺血性脑病患儿与正常足月新生儿外周静脉血中淋巴细胞(Peripheral blood lymphocytes cell,PBLC)中钟基因Clock m RNA、Bmal1 m RNA含量水平的变化,研究探讨HIE所致的昼夜节律紊乱中对钟基因的影响。方法:采用半...目的:通过比较足月缺氧缺血性脑病患儿与正常足月新生儿外周静脉血中淋巴细胞(Peripheral blood lymphocytes cell,PBLC)中钟基因Clock m RNA、Bmal1 m RNA含量水平的变化,研究探讨HIE所致的昼夜节律紊乱中对钟基因的影响。方法:采用半定量逆转录聚合酶链反应(Reverse Transcriptase Polymerase Chain Reaction,RT-PCR)测定32例缺氧缺血性脑病患儿(HIE组)与24例正常足月新生儿(对照组)外周血淋巴细胞中Clock m RNA、Bmal1 m RNA的含量,并比较各组之间的差异。结果:HIE组外周血淋巴细胞中Clock m RNA和Bmal1 m RNA的水平均低于对照组,两组比较差异有统计学意义(P<0.05),HIE组外周血淋巴细胞中Clock m RNA、Bmal1 m RNA含量的变化趋势基本一致。结论:HIE患儿PBLC中Clock m RNA、Bmal1 m RNA表达存在异常,两者可能在缺氧缺血后昼夜节律紊乱的发生起着一定的作用。展开更多
There is an impressive scarcity of quantitative models of the clock patterns in the brain. We propose a mesoscopic approach, i.e. neither a description at single neuron level, nor at systemic level/too coarse granular...There is an impressive scarcity of quantitative models of the clock patterns in the brain. We propose a mesoscopic approach, i.e. neither a description at single neuron level, nor at systemic level/too coarse granularity, of the time perception at the time of the saccade. This model uses functional pathway knowledge and is inspired by, and integrates, recent findings in both psychophysics and neurophysiology. Perceived time delays in the perisaccadic window are shown numerically consistent with recent experimental measures. Our model provides explanation for several experimental outcomes on saccades, estimates popu-lation variance of the error in time perception and represent a meaningful example for bridging psychophysics and neurophysiology. Finally we found that the insights into information processing during saccadic events lead to considerations on engineering exploitation of the underlying phenomena.展开更多
基金supported by grants from the Foundation for Advancing Medical Sciences of the Health Department, Jiangsu Province, No. Z200519the Project for Social Development of Suzhou, No. SSZ0230
文摘Clock genes are involved in circadian rhythm regulation, and surviving newborns with hypoxic-ischemic encephalopathy may present with sleep-wake cycle reversal. This study aimed to determine the expression of the clock genes Clock and Bmall, in the pineal gland of rats with hypoxic-ischemic brain damage. Results showed that levels of Clock mRNA v^re not significantly changed within 48 hours after cerebral hypoxia and ischemia. Expression levels of CLOCK and BMAL1 protein were significantly higher after 48 hours. The levels of Bmall mRNA reached a peak at 36 hours, but were significantly reduced at 48 hours. Experimental findings indicate that Clock and Bmall genes were indeed expressed in the pineal glands of neonatal rats. At the initial stage （within 36 hours） of hypoxic-ischemic brain damage, only slight changes in the expression levels of these two genes were detected, followed by significant changes at 36-48 hours. These changes may be associated with circadian rhythm disorder induced by hypoxic-ischemic brain damage.
文摘目的:通过比较足月缺氧缺血性脑病患儿与正常足月新生儿外周静脉血中淋巴细胞(Peripheral blood lymphocytes cell,PBLC)中钟基因Clock m RNA、Bmal1 m RNA含量水平的变化,研究探讨HIE所致的昼夜节律紊乱中对钟基因的影响。方法:采用半定量逆转录聚合酶链反应(Reverse Transcriptase Polymerase Chain Reaction,RT-PCR)测定32例缺氧缺血性脑病患儿(HIE组)与24例正常足月新生儿(对照组)外周血淋巴细胞中Clock m RNA、Bmal1 m RNA的含量,并比较各组之间的差异。结果:HIE组外周血淋巴细胞中Clock m RNA和Bmal1 m RNA的水平均低于对照组,两组比较差异有统计学意义(P<0.05),HIE组外周血淋巴细胞中Clock m RNA、Bmal1 m RNA含量的变化趋势基本一致。结论:HIE患儿PBLC中Clock m RNA、Bmal1 m RNA表达存在异常,两者可能在缺氧缺血后昼夜节律紊乱的发生起着一定的作用。
基金supported by the Natural Science Foundation of Hubei ProvinceChina(No.2019CFB758)+1 种基金the National Natural Science Foundation of China(No.31772454)the Fundamental Research Funds of Central China Normal University(No.CCNU20TS018)。
文摘There is an impressive scarcity of quantitative models of the clock patterns in the brain. We propose a mesoscopic approach, i.e. neither a description at single neuron level, nor at systemic level/too coarse granularity, of the time perception at the time of the saccade. This model uses functional pathway knowledge and is inspired by, and integrates, recent findings in both psychophysics and neurophysiology. Perceived time delays in the perisaccadic window are shown numerically consistent with recent experimental measures. Our model provides explanation for several experimental outcomes on saccades, estimates popu-lation variance of the error in time perception and represent a meaningful example for bridging psychophysics and neurophysiology. Finally we found that the insights into information processing during saccadic events lead to considerations on engineering exploitation of the underlying phenomena.
