Malus prunifolia Borkh. ‘Fupingqiuzi’ has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are...Malus prunifolia Borkh. ‘Fupingqiuzi’ has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are barriers to the identification of ‘Fupingqiuzi’ progeny with excellent traits. In-vitro regeneration techniques and Agrobacterium-mediated genetic transformation systems can efficiently produce complete plants and thus enable studies of gene function.However, optimal regeneration and genetic transformation systems for ‘Fupingqiuzi’ have not yet been developed.Here, we evaluated the factors that affect the in-vitro regeneration and transformation of ‘Fupingqiuzi’. The best results were obtained when transverse leaf sections were used as explants, and they were grown in dark culture for three weeks with their adaxial sides contacting the culture medium(MS basal salts, 30 g Lsucrose, 8 g Lagar, 5 mg L6-benzylaminopurine(6-BA), 2 mg Lthidiazuron(TDZ), and 1 mg L1-naphthlcetic acid(NAA), pH 5.8). A genetic transformation system based on this regeneration system was optimized: after inoculation with A. tumefaciens solution for 8 min, 4 days of co-culture, and 3 days of delayed culture, the cultures were screened with cefotaxime(150 mg L) and kanamycin(15 mg L). We thus established an efficient regeneration and genetic transformation system for ‘Fupingqiuzi’, enabling the rapid production of transgenic material. These findings make a significant contribution to apple biology research.展开更多
[ Objective] This study aimed to analyze the dynamics of growth and development of Armeniaca dasycarpa （ Ehrh. ） Borkh fruit, thus providing the the- oretical basis for cultivation and breeding of good-quality and h...[ Objective] This study aimed to analyze the dynamics of growth and development of Armeniaca dasycarpa （ Ehrh. ） Borkh fruit, thus providing the the- oretical basis for cultivation and breeding of good-quality and high-yield A. dasycarpa. [ Method] With A. dasycarpa cultivar Yechengzixing as the experimental material, P. cerasifera Ehrh cultivar Huangguoyingtaoli and A. vulgaris Lam. cultivars Luopuhongdaike, Wanshujianali and Kuchetuoyong as control, the vertical diameter, horizontal diameter and fresh weight of fruit and embryos were measured every 7 d during the growth and development period. [ Result] The result showed that the whole fruit growth period of Yechengzixing, lasted 93 d from the end of flowering to fruit maturity. The dynamics of fruit vertical diameter, horizon- tal diameter and fresh weight of Yechengzixing presented ＂rapid-slow-rapid＂ double S-shaped curves. The whole fruit growth period of Yechengzixing could be di- vided approximately into three stages： first rapid growth stage, slow growth stage and second rapid growth stage, which lasted 29, 42 and 21 d, respectively. Daily increment of fruit vertical diameter and horizontal diameter of Yechengzixing reached the maximum at the first rapid growth stage, while daily increment of fruit fresh weight reached the maximum at the second rapid growth stage. The dynamics of vertical diameter, horizontal diameter and fresh weight of Yechengzixing embryos presented S-shaped curves. Fruit growth and development dynamics of the tested materials presented double S-shaped curves. The whole fruit growth period of Yechengzixing, Huangguoyingtaoli and three apricot cuhivars lasted 93, 119 and 77 -98 d, respectively. Thus, the duration of fruit growth period of Yechengzixing was 26 d shorter than that of Huangguoyingtaoli and exhibited no significant difference compared with three apricot cultivars. The duration of each growth stage and daily increment of Yechengzixing fruit had no significant difference compared with three apricot cultivars. Moreover, Huangguoyingtaoli had a longer slow growth stage and lower daily increment than Yechengzixing. [ Conclusion] The whole fruit growth period, duration of each growth stage and daily increment of Yechengzix- ing exhibited no significant difference compared with three apricot cultivars but varied remarkably compared with Huangguoyingtaoli.展开更多
基金supported by the National Key Research and Development Program of China(2019YFD1001403-4)the Key S&T Special Projects of Shaanxi Province,China(2020zdzx03-01-02)the earmarked fund for the China Agriculture Research System of MOF and MARA(CARS-27)。
文摘Malus prunifolia Borkh. ‘Fupingqiuzi’ has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are barriers to the identification of ‘Fupingqiuzi’ progeny with excellent traits. In-vitro regeneration techniques and Agrobacterium-mediated genetic transformation systems can efficiently produce complete plants and thus enable studies of gene function.However, optimal regeneration and genetic transformation systems for ‘Fupingqiuzi’ have not yet been developed.Here, we evaluated the factors that affect the in-vitro regeneration and transformation of ‘Fupingqiuzi’. The best results were obtained when transverse leaf sections were used as explants, and they were grown in dark culture for three weeks with their adaxial sides contacting the culture medium(MS basal salts, 30 g Lsucrose, 8 g Lagar, 5 mg L6-benzylaminopurine(6-BA), 2 mg Lthidiazuron(TDZ), and 1 mg L1-naphthlcetic acid(NAA), pH 5.8). A genetic transformation system based on this regeneration system was optimized: after inoculation with A. tumefaciens solution for 8 min, 4 days of co-culture, and 3 days of delayed culture, the cultures were screened with cefotaxime(150 mg L) and kanamycin(15 mg L). We thus established an efficient regeneration and genetic transformation system for ‘Fupingqiuzi’, enabling the rapid production of transgenic material. These findings make a significant contribution to apple biology research.
基金Supported by Science and Technology Project of Xinjiang Uygur Autonomous Region(201130102)Special Fund for Scientific Research in the Public Interest(201304701)Key Discipline Fund of Pomology of Xinjiang Uygur Autonomous Region
文摘[ Objective] This study aimed to analyze the dynamics of growth and development of Armeniaca dasycarpa （ Ehrh. ） Borkh fruit, thus providing the the- oretical basis for cultivation and breeding of good-quality and high-yield A. dasycarpa. [ Method] With A. dasycarpa cultivar Yechengzixing as the experimental material, P. cerasifera Ehrh cultivar Huangguoyingtaoli and A. vulgaris Lam. cultivars Luopuhongdaike, Wanshujianali and Kuchetuoyong as control, the vertical diameter, horizontal diameter and fresh weight of fruit and embryos were measured every 7 d during the growth and development period. [ Result] The result showed that the whole fruit growth period of Yechengzixing, lasted 93 d from the end of flowering to fruit maturity. The dynamics of fruit vertical diameter, horizon- tal diameter and fresh weight of Yechengzixing presented ＂rapid-slow-rapid＂ double S-shaped curves. The whole fruit growth period of Yechengzixing could be di- vided approximately into three stages： first rapid growth stage, slow growth stage and second rapid growth stage, which lasted 29, 42 and 21 d, respectively. Daily increment of fruit vertical diameter and horizontal diameter of Yechengzixing reached the maximum at the first rapid growth stage, while daily increment of fruit fresh weight reached the maximum at the second rapid growth stage. The dynamics of vertical diameter, horizontal diameter and fresh weight of Yechengzixing embryos presented S-shaped curves. Fruit growth and development dynamics of the tested materials presented double S-shaped curves. The whole fruit growth period of Yechengzixing, Huangguoyingtaoli and three apricot cuhivars lasted 93, 119 and 77 -98 d, respectively. Thus, the duration of fruit growth period of Yechengzixing was 26 d shorter than that of Huangguoyingtaoli and exhibited no significant difference compared with three apricot cultivars. The duration of each growth stage and daily increment of Yechengzixing fruit had no significant difference compared with three apricot cultivars. Moreover, Huangguoyingtaoli had a longer slow growth stage and lower daily increment than Yechengzixing. [ Conclusion] The whole fruit growth period, duration of each growth stage and daily increment of Yechengzix- ing exhibited no significant difference compared with three apricot cultivars but varied remarkably compared with Huangguoyingtaoli.
