Low temperature(LT)in spring has become one of the principal abiotic stresses that restrict the growth and development of wheat.Diverse analyses were performed to investigate the mechanism underlying the response of w...Low temperature(LT)in spring has become one of the principal abiotic stresses that restrict the growth and development of wheat.Diverse analyses were performed to investigate the mechanism underlying the response of wheat grain development to LT stress during booting.These included morphological observation,measurements of starch synthase activity,and determination of amylose and amylopectin content of wheat grain after exposure to treatment with LT during booting.Additionally,proteomic analysis was performed using tandem mass tags(TMT).Results showed that the plumpness of wheat grains decreased after LT stress.Moreover,the activities of sucrose synthase(SuS,EC 2.4.1.13)and ADP-glucose pyrophosphorylase(AGPase,EC 2.7.7.27)exhibited a significant reduction,leading to a significant reduction in the contents of amylose and amylopectin.A total of 509 differentially expressed proteins(DEPs)were identified by proteomics analysis.The Gene Ontology(GO)enrichment analysis showed that the protein difference multiple in the nutritional repository activity was the largest among the molecular functions,and the up-regulated seed storage protein(ssP)played an active role in the response of grains to LT stress and subsequent damage.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that LT stress reduced the expression of DEPs such as sucrose phosphate synthase(SPS),glucose-1-phosphate adenylyltransferase(glgC),andβ-fructofuranosidase(FFase)in sucrose and starch metabolic pathways,thus affecting the synthesis of grain starch.In addition,many heat shock proteins(HsPs)were found in the protein processing in endoplasmic reticulum pathways,which can resist some damage caused by LT stress.These findings provide a new theoretical foundation for elucidating the underlying mechanism governing wheat yield developmentafterexposuretoLTstress inspring.展开更多
According to phenological observation data of fourteen stations in Hunan,Hubei and Jiangxi provinces from 1994 to 2006,indices of suitable temperature at the booting stage and accumulated temperature(≥10 ℃)during th...According to phenological observation data of fourteen stations in Hunan,Hubei and Jiangxi provinces from 1994 to 2006,indices of suitable temperature at the booting stage and accumulated temperature(≥10 ℃)during the whole growth period in early or late rice in the south of China were analyzed by statistics and biological relationship between factors.Indices of the optimum daily mean temperature and the minimum temperature at the booting stage were as follows:Early convention rice variety was 25.7 ℃ and 22.3 ℃ respectively,hybrid variety was 26.5 ℃ and 23.3℃ respectively;while late convention rice variety was 25.6 ℃ and 22.3 ℃ respectively,hybrid variety was 25.8 ℃ and 22.6 ℃ respectively.Indexes of accumulated temperature during the whole growth period were as follows:The early-maturing variety,the middle-maturing variety and the late-maturing variety of early rice was 2 512.1 ℃,2 528.4 ℃ and 2 651.3 ℃ respectively,while the early-maturing variety,the middle-maturing variety and the late-maturing variety of late rice was 3 126.2 ℃,3 031.5 ℃ and 3 335.2 ℃ respectively.The above indexes all met the requirements of identification,which could provide reference for formulating the meteorological standard of rice in the south of China.展开更多
[Objective] The physiological characteristics and yield compensation effects of rice in booting stage were studied after water recovery when rice suffered water stress to provide references for improving rice water ut...[Objective] The physiological characteristics and yield compensation effects of rice in booting stage were studied after water recovery when rice suffered water stress to provide references for improving rice water utilization efficiency and its drought resistance. [Method] The pot experiments with five rice varieties under three different treatments of water stresses were conducted to study physical signs every six days after water water-recovery. [Result] Along with the increase of water recov- ery, leaf water potential, SPAD and Pn values in treatment B （light drought） in- creased fast, and its compensation effect of the soluble sugar content was higher than that of treatment C （severe drought）; all sap flow speeds in treatments with water stresses fell. As for yield components, panicles per plant and spikelets per panicle decreased with water stress degree, and the decline of spikelets per pani- cle, in particular, was greatly influenced by water stress. Seed setting percentages in treatment B most reached peaks of different varieties, except of D you No. 363. The thousand-seed weight and ear weight per plant in treatment B and C both dropped, compared with control group. [Conclusion] When regular management was resumed after rice underwent water stress at booting stage, many characteristics and yield traits of rice got favorable compensation effects in the light water-stress treatments.展开更多
Genetic analysis showed that cold tolerance at booting stage of near-isogenic lines （NILs） of Kunmingxiaobaigu was controlled by a gene with large phenotypic variance. One hundred and sixty-four simple sequence repe...Genetic analysis showed that cold tolerance at booting stage of near-isogenic lines （NILs） of Kunmingxiaobaigu was controlled by a gene with large phenotypic variance. One hundred and sixty-four simple sequence repeats （SSR） distributed over 12 chromosomes were used to screen polymorphism between Towata （recurrent parent, RP） and near-isogenic line pool （NILP）, and two SSR markers at the long arm of chromosome 5 showed polymorphism in comparison with RP genome. Of the two markers, RM31 was found possibly linked with the cold tolerance gene at booting stage through one-way ANOVA. Twelve SSR markers around RM31 were then used to detect polymorphism between RP and NIL, and only RM7452 had polymorphism. The gene of cold tolerance at booting stage was further mapped on chromosome 5 between RM7452 and RM31 with genetic distances of 4.8 cM and 8.0 cM, respectively. This gene explained 10.50% of phenotypic variance and 5.10% of phenotypic variance of fully filled grains, and was tentatively designated as Ctb（t）.展开更多
Internet-based virtual computing environment （iVCE） has been proposed to combine data centers and other kinds of computing resources on the Internet to provide efficient and economical services. Virtual machines （...Internet-based virtual computing environment （iVCE） has been proposed to combine data centers and other kinds of computing resources on the Internet to provide efficient and economical services. Virtual machines （VMs） have been widely used in iVCE to isolate different users/jobs and ensure trustworthiness, but traditionally VMs require a long period of time for booting, which cannot meet the requirement of iVCE＇s large-scale and highly dynamic applications. To address this problem, in this paper we design and implement VirtMan, a fast booting system for a large number of virtual machines in iVCE. VirtMan uses the Linux Small Computer System Interface （SCSI） target to remotely mount to the source image in a scalable hierarchy, and leverages the homogeneity of a set of VMs to transfer only necessary image data at runtime. We have implemented VirtMan both as a standalone system and for OpenStack. In our 100-server testbed, VirtMan boots up 1000 VMs （with a 15 CB image of Windows Server 2008） on 100 physical servers in less than 120 s, which is three orders of magnitude lower than current public clouds.展开更多
基金supported by the National Natural Science Foundation of China(32372223)the National Key Research and Development Program of China(2022YFD2301404)+1 种基金the College Students'Innovationand Entrepreneurship Training Program of Anhui Province,China(S202210364136)the Natural Science Research Project of Anhui Educational Committee,China(2023AH040133).
文摘Low temperature(LT)in spring has become one of the principal abiotic stresses that restrict the growth and development of wheat.Diverse analyses were performed to investigate the mechanism underlying the response of wheat grain development to LT stress during booting.These included morphological observation,measurements of starch synthase activity,and determination of amylose and amylopectin content of wheat grain after exposure to treatment with LT during booting.Additionally,proteomic analysis was performed using tandem mass tags(TMT).Results showed that the plumpness of wheat grains decreased after LT stress.Moreover,the activities of sucrose synthase(SuS,EC 2.4.1.13)and ADP-glucose pyrophosphorylase(AGPase,EC 2.7.7.27)exhibited a significant reduction,leading to a significant reduction in the contents of amylose and amylopectin.A total of 509 differentially expressed proteins(DEPs)were identified by proteomics analysis.The Gene Ontology(GO)enrichment analysis showed that the protein difference multiple in the nutritional repository activity was the largest among the molecular functions,and the up-regulated seed storage protein(ssP)played an active role in the response of grains to LT stress and subsequent damage.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that LT stress reduced the expression of DEPs such as sucrose phosphate synthase(SPS),glucose-1-phosphate adenylyltransferase(glgC),andβ-fructofuranosidase(FFase)in sucrose and starch metabolic pathways,thus affecting the synthesis of grain starch.In addition,many heat shock proteins(HsPs)were found in the protein processing in endoplasmic reticulum pathways,which can resist some damage caused by LT stress.These findings provide a new theoretical foundation for elucidating the underlying mechanism governing wheat yield developmentafterexposuretoLTstress inspring.
基金Supported by Project for NewTechnology Generalization of China Meteorological Administration(CMATG2007M37)~~
文摘According to phenological observation data of fourteen stations in Hunan,Hubei and Jiangxi provinces from 1994 to 2006,indices of suitable temperature at the booting stage and accumulated temperature(≥10 ℃)during the whole growth period in early or late rice in the south of China were analyzed by statistics and biological relationship between factors.Indices of the optimum daily mean temperature and the minimum temperature at the booting stage were as follows:Early convention rice variety was 25.7 ℃ and 22.3 ℃ respectively,hybrid variety was 26.5 ℃ and 23.3℃ respectively;while late convention rice variety was 25.6 ℃ and 22.3 ℃ respectively,hybrid variety was 25.8 ℃ and 22.6 ℃ respectively.Indexes of accumulated temperature during the whole growth period were as follows:The early-maturing variety,the middle-maturing variety and the late-maturing variety of early rice was 2 512.1 ℃,2 528.4 ℃ and 2 651.3 ℃ respectively,while the early-maturing variety,the middle-maturing variety and the late-maturing variety of late rice was 3 126.2 ℃,3 031.5 ℃ and 3 335.2 ℃ respectively.The above indexes all met the requirements of identification,which could provide reference for formulating the meteorological standard of rice in the south of China.
基金Supported by Capacity Construction of Rice Breeding,Cultivation,and Industrialization Innovation Project in Guizhou([2011]4003)Rice Scientific Research Infrastructure Building Project in Mountainous Regions of Guizhou([2011]4005)Rice Genetics and Breeding Research and Innovation Team in Guizhou([2012]4020)~~
文摘[Objective] The physiological characteristics and yield compensation effects of rice in booting stage were studied after water recovery when rice suffered water stress to provide references for improving rice water utilization efficiency and its drought resistance. [Method] The pot experiments with five rice varieties under three different treatments of water stresses were conducted to study physical signs every six days after water water-recovery. [Result] Along with the increase of water recov- ery, leaf water potential, SPAD and Pn values in treatment B （light drought） in- creased fast, and its compensation effect of the soluble sugar content was higher than that of treatment C （severe drought）; all sap flow speeds in treatments with water stresses fell. As for yield components, panicles per plant and spikelets per panicle decreased with water stress degree, and the decline of spikelets per pani- cle, in particular, was greatly influenced by water stress. Seed setting percentages in treatment B most reached peaks of different varieties, except of D you No. 363. The thousand-seed weight and ear weight per plant in treatment B and C both dropped, compared with control group. [Conclusion] When regular management was resumed after rice underwent water stress at booting stage, many characteristics and yield traits of rice got favorable compensation effects in the light water-stress treatments.
基金supportedby China National Natural Science Foundation(30160043,30260060) the Natural ScienceFoundation of Yunnan Province(2004CO010Z).
文摘Genetic analysis showed that cold tolerance at booting stage of near-isogenic lines （NILs） of Kunmingxiaobaigu was controlled by a gene with large phenotypic variance. One hundred and sixty-four simple sequence repeats （SSR） distributed over 12 chromosomes were used to screen polymorphism between Towata （recurrent parent, RP） and near-isogenic line pool （NILP）, and two SSR markers at the long arm of chromosome 5 showed polymorphism in comparison with RP genome. Of the two markers, RM31 was found possibly linked with the cold tolerance gene at booting stage through one-way ANOVA. Twelve SSR markers around RM31 were then used to detect polymorphism between RP and NIL, and only RM7452 had polymorphism. The gene of cold tolerance at booting stage was further mapped on chromosome 5 between RM7452 and RM31 with genetic distances of 4.8 cM and 8.0 cM, respectively. This gene explained 10.50% of phenotypic variance and 5.10% of phenotypic variance of fully filled grains, and was tentatively designated as Ctb（t）.
基金supported by the National Natural Science Foundation of China(Nos.61379055 and 61379053)
文摘Internet-based virtual computing environment （iVCE） has been proposed to combine data centers and other kinds of computing resources on the Internet to provide efficient and economical services. Virtual machines （VMs） have been widely used in iVCE to isolate different users/jobs and ensure trustworthiness, but traditionally VMs require a long period of time for booting, which cannot meet the requirement of iVCE＇s large-scale and highly dynamic applications. To address this problem, in this paper we design and implement VirtMan, a fast booting system for a large number of virtual machines in iVCE. VirtMan uses the Linux Small Computer System Interface （SCSI） target to remotely mount to the source image in a scalable hierarchy, and leverages the homogeneity of a set of VMs to transfer only necessary image data at runtime. We have implemented VirtMan both as a standalone system and for OpenStack. In our 100-server testbed, VirtMan boots up 1000 VMs （with a 15 CB image of Windows Server 2008） on 100 physical servers in less than 120 s, which is three orders of magnitude lower than current public clouds.
