Bone healing is integral to orthopedic research,focusing on the restoration of bone function through a complex interplay of inflammatory responses,soft callus formation,hard callus development,and the final remodeling...Bone healing is integral to orthopedic research,focusing on the restoration of bone function through a complex interplay of inflammatory responses,soft callus formation,hard callus development,and the final remodeling phase.While the natural progression of bone healing is a finely tuned process,it can be disrupted by inflammatory dysregulation,ranging from chronic inflammation to acute inflammatory anomalies,and by the depletion of essential repair substances under both chronic and acute conditions.Current strategies to enhance bone healing employ a multifaceted approach,including biochemical modulation of the local microenvironment through essential nutrient supplementation(e.g.,calcium and vitamin D),biomechanical optimization via improved internal fixation stability,and advanced regenerative techniques incorporating bioactive factors,stem cell therapies,and functional biomaterials.Despite these efforts,challenges persist in the precise characterization of the local microenvironment and the precise control of in vivo bioactive molecule delivery.This review comprehensively summarizes the current research progress in bone healing,providing significant reference for understanding the mechanisms of bone healing and for guiding further research.It is expected to lay the theoretical foundation for the development of more effective therapeutic strategies for bone healing.展开更多
Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich pla...Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich plasma （PRP） on long bone defect restoration.Methods：A radial bone defect model was constructed through an osteotomy using New Zealand rabbits.The rabbits were randomly divided into four groups （n =10 in each group）：a CS combined with PRP （CS-PRP） group,a CS group,a PRP group,and a positive （recombinant human bone morphogenetic protein-2） control group.PRP was prepared from autologous blood using a two-step centrifugation process.CS-PRP was obtained by mixing hemihydrate CS with PRP.Radiographs and histologic micrographs were generated.The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks.One-way analysis of variance was performed in this study.Results：The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups,while nonunion was observed in the CS and PRP groups.The percentages of bone regenerated bone area in the CS-PRP （84.60 ± 2.87%） and positive control （52.21 ± 4.53%） groups were significantly greater than those in the CS group （12.34 ± 2.17%） and PRP group （16.52 ± 4.22%） （P 〈 0.001）.In addition,the bone strength of CS-PRP group （43.l 0 ± 4.10%） was significantly greater than that of the CS group （20.10 ± 3.70%） or PRP group （25.10 ± 2.10%） （P 〈 0.001）.Conclusion：CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength.展开更多
基金supported by funding from the National Key Research and Development Program of China(2022YFC2504300)the Joint Co-construction Project of Medical Science and Technology Tackling Program in Henan Province(LHGJ20240248)+1 种基金the Key R&D and Promotion Projects in Henan Province of China(242102311139)the 2024 Postdoctoral Research Initiation Grant for the First Affiliated Hospital of Zhengzhou University(72402)。
文摘Bone healing is integral to orthopedic research,focusing on the restoration of bone function through a complex interplay of inflammatory responses,soft callus formation,hard callus development,and the final remodeling phase.While the natural progression of bone healing is a finely tuned process,it can be disrupted by inflammatory dysregulation,ranging from chronic inflammation to acute inflammatory anomalies,and by the depletion of essential repair substances under both chronic and acute conditions.Current strategies to enhance bone healing employ a multifaceted approach,including biochemical modulation of the local microenvironment through essential nutrient supplementation(e.g.,calcium and vitamin D),biomechanical optimization via improved internal fixation stability,and advanced regenerative techniques incorporating bioactive factors,stem cell therapies,and functional biomaterials.Despite these efforts,challenges persist in the precise characterization of the local microenvironment and the precise control of in vivo bioactive molecule delivery.This review comprehensively summarizes the current research progress in bone healing,providing significant reference for understanding the mechanisms of bone healing and for guiding further research.It is expected to lay the theoretical foundation for the development of more effective therapeutic strategies for bone healing.
文摘Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich plasma （PRP） on long bone defect restoration.Methods：A radial bone defect model was constructed through an osteotomy using New Zealand rabbits.The rabbits were randomly divided into four groups （n =10 in each group）：a CS combined with PRP （CS-PRP） group,a CS group,a PRP group,and a positive （recombinant human bone morphogenetic protein-2） control group.PRP was prepared from autologous blood using a two-step centrifugation process.CS-PRP was obtained by mixing hemihydrate CS with PRP.Radiographs and histologic micrographs were generated.The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks.One-way analysis of variance was performed in this study.Results：The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups,while nonunion was observed in the CS and PRP groups.The percentages of bone regenerated bone area in the CS-PRP （84.60 ± 2.87%） and positive control （52.21 ± 4.53%） groups were significantly greater than those in the CS group （12.34 ± 2.17%） and PRP group （16.52 ± 4.22%） （P 〈 0.001）.In addition,the bone strength of CS-PRP group （43.l 0 ± 4.10%） was significantly greater than that of the CS group （20.10 ± 3.70%） or PRP group （25.10 ± 2.10%） （P 〈 0.001）.Conclusion：CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength.
