The Brassica polima cytoplasmic male sterility(pol CMS)line causes complete and stable sterility and is most extensively used in breeding.The regulatory pathway,however,is not clear.In this work,we studied molecular i...The Brassica polima cytoplasmic male sterility(pol CMS)line causes complete and stable sterility and is most extensively used in breeding.The regulatory pathway,however,is not clear.In this work,we studied molecular interaction among several key genes involved in pol CMS.Firstly,we found that the multicellular organelle RNA-editing factor protein(Bna.MORF1)interacted with the pol CMS-restorer protein RFP using the yeast two-hybrid system.Secondly,knock down of Bna.MORF1 using CRISPR/Cas9 editing resulted in sterile transgenic lines.The function of the pol CMS sterility gene orf224 was further confirmed by ectopic expression of the gene in both Arabidopsis and Brassica.Furthermore,using CRISPR/Cas9 we determined that an anther-specific proline-rich protein(APG)was also involved in sterility.We propose a working model for pol CMS in Brassica napus that may expedite the utilization of this popular CMS line in Brassica breeding.展开更多
基金supported by the Wuhan Science and Technology Major Project on Key Techniques of Biological Breeding and Breeding of New Varieties(2022021302024851)the National Key Research and Development Program of China(2021YFD1600500)the National Natural Science Foundation of China(32201883)。
文摘The Brassica polima cytoplasmic male sterility(pol CMS)line causes complete and stable sterility and is most extensively used in breeding.The regulatory pathway,however,is not clear.In this work,we studied molecular interaction among several key genes involved in pol CMS.Firstly,we found that the multicellular organelle RNA-editing factor protein(Bna.MORF1)interacted with the pol CMS-restorer protein RFP using the yeast two-hybrid system.Secondly,knock down of Bna.MORF1 using CRISPR/Cas9 editing resulted in sterile transgenic lines.The function of the pol CMS sterility gene orf224 was further confirmed by ectopic expression of the gene in both Arabidopsis and Brassica.Furthermore,using CRISPR/Cas9 we determined that an anther-specific proline-rich protein(APG)was also involved in sterility.We propose a working model for pol CMS in Brassica napus that may expedite the utilization of this popular CMS line in Brassica breeding.
