目的:观察电针对兔激素性股骨头坏死血清中成血管因子HIF-1α、VEGF;成骨因子BMP2、Osterix;H型血管标志物CD31;骨破坏相关因子MMP13的影响,探索电针治疗激素性股骨头坏死的治疗作用及机制。方法:选取健康雄性新西兰大白兔18只,随机分...目的:观察电针对兔激素性股骨头坏死血清中成血管因子HIF-1α、VEGF;成骨因子BMP2、Osterix;H型血管标志物CD31;骨破坏相关因子MMP13的影响,探索电针治疗激素性股骨头坏死的治疗作用及机制。方法:选取健康雄性新西兰大白兔18只,随机分成正常组、模型组、电针组(电针环跳穴、髀关穴),每组6只。运用qRT-PCR试剂盒检测兔血清中HIF-1α、VEGF、BMP2、Osterix含量。运用免疫荧光染色检测H型血管标志物CD31、骨破坏相关因子MMP13表达情况。结果:HIF-1α、VEGF、BMP2、Osterix的含量,模型组浓度明显低于正常组(P α、BMP2、Osterix、CD31的表达,从而造成骨质破坏和血管破坏,导致股骨头坏死。电针能调控成血管相关因子VEGF、HIF-α,成骨相关因子BMP2、Osterix,H型血管标志物CD31,使VEGF、HIF-α、BMP2、Osterix、CD31浓度均增高,促进股骨头内血管新生,骨破坏相关因子MMP13表达量下降,从而增强骨组织自身修复能力。这可能是电针治疗激素性股骨头坏死的机制之一。Objective: To observe the effect of electroacupuncture on angiogenic factors HIF-1α and VEGF;osteogenic factors BMP2 and Osterix;H-type vascular marker CD31;and bone destruction-related factor MMP13 in the serum of rabbits with steroid-induced femoral head necrosis, and to explore the therapeutic effect and mechanism of electroacupuncture in the treatment of steroid-induced femoral head necrosis. Methods: Eighteen healthy male New Zealand white rabbits were randomly divided into a normal group, a model group, and an electroacupuncture group (electroacupuncture at Huantiao acupoint and Biguan acupoint), with 6 rabbits in each group. The levels of HIF-1α, VEGF, BMP2, and Osterix in rabbit serum were detected by qRT-PCR kit. Immunofluorescence staining was used to detect the expression of H-type vascular marker CD31 and bone destruction-related factor MMP13. Results: The concentrations of HIF-1α, VEGF, BMP2, and Osterix in the model group were significantly lower than those in the normal group (P α, BMP2, Osterix, and CD31, thereby causing bone destruction and vascular destruction, leading to femoral head necrosis. Electroacupuncture can regulate angiogenesis-related factors VEGF, HIF-α, osteogenic-related factors BMP2, Osterix, and H-type vascular marker CD31, increasing the concentrations of VEGF, HIF-α, BMP2, Osterix, and CD31, promoting angiogenesis in the femoral head, and reducing the expression of bone destruction-related factor MMP13, thereby enhancing the self-repair ability of bone tissue. This may be one of the mechanisms of electroacupuncture treatment of steroid-induced femoral head necrosis.展开更多
目的该研究利用叶酸拮抗剂甲氨喋呤（MTX）构建叶酸生物学活性受抑制的斑马鱼模型后，观察叶酸生物学活性受抑后对斑马鱼心脏发育的干扰作用以及对斑马鱼心脏发育相关基因BMP2b及HAS2表达的影响。方法用不同浓度的MTX处理不同发育时段...目的该研究利用叶酸拮抗剂甲氨喋呤（MTX）构建叶酸生物学活性受抑制的斑马鱼模型后，观察叶酸生物学活性受抑后对斑马鱼心脏发育的干扰作用以及对斑马鱼心脏发育相关基因BMP2b及HAS2表达的影响。方法用不同浓度的MTX处理不同发育时段的斑马鱼胚胎，于48hpf（hours post fertilization）观察胚胎心脏发育情况并计数各组心脏发育异常个体的百分比及心率，评定MTX对斑马鱼心脏发育的影响程度。用1．5×10^-3M的MTX处理6～10hpf发育时段的斑马鱼胚胎作为MTX处理组。于24hpf及48hpf在显微镜下观察MTX处理组斑马鱼胚胎心脏发育情况。借助胚胎整体原位杂交和Real-time PCR的方法检测BMP2b和HAS2在正常对照组及MTX处理组胚胎的表达水平。结果胚胎早期发育阶段6～12hpf是斑马鱼胚胎对MTX的敏感时期。显微镜下观察结果显示MTX处理组斑马鱼心脏发育延迟，并有心脏形态发育明显异常。胚胎整体原位杂交结果显示MTX处理组斑马鱼心脏发育相关基因BMP2b及HAS2在心脏的表达于36hpf及48hpf下调。Real-time PCR结果显示MTX处理组斑马鱼BMP2b的相对表达量在12，24，36及48hpf减少，HAS2的相对表达量在24，36及48hpf减少。结论叶酸生物学活性受抑对早期胚胎的心脏发育影响较大，可导致斑马鱼心脏发育延迟及心脏形态异常，并下调斑马鱼心脏发育相关基因BMP2b及HAS2的表达，这可能是叶酸生物学活性受抑后导致心脏发育异常的机制之一。展开更多
文摘目的:观察电针对兔激素性股骨头坏死血清中成血管因子HIF-1α、VEGF;成骨因子BMP2、Osterix;H型血管标志物CD31;骨破坏相关因子MMP13的影响,探索电针治疗激素性股骨头坏死的治疗作用及机制。方法:选取健康雄性新西兰大白兔18只,随机分成正常组、模型组、电针组(电针环跳穴、髀关穴),每组6只。运用qRT-PCR试剂盒检测兔血清中HIF-1α、VEGF、BMP2、Osterix含量。运用免疫荧光染色检测H型血管标志物CD31、骨破坏相关因子MMP13表达情况。结果:HIF-1α、VEGF、BMP2、Osterix的含量,模型组浓度明显低于正常组(P α、BMP2、Osterix、CD31的表达,从而造成骨质破坏和血管破坏,导致股骨头坏死。电针能调控成血管相关因子VEGF、HIF-α,成骨相关因子BMP2、Osterix,H型血管标志物CD31,使VEGF、HIF-α、BMP2、Osterix、CD31浓度均增高,促进股骨头内血管新生,骨破坏相关因子MMP13表达量下降,从而增强骨组织自身修复能力。这可能是电针治疗激素性股骨头坏死的机制之一。Objective: To observe the effect of electroacupuncture on angiogenic factors HIF-1α and VEGF;osteogenic factors BMP2 and Osterix;H-type vascular marker CD31;and bone destruction-related factor MMP13 in the serum of rabbits with steroid-induced femoral head necrosis, and to explore the therapeutic effect and mechanism of electroacupuncture in the treatment of steroid-induced femoral head necrosis. Methods: Eighteen healthy male New Zealand white rabbits were randomly divided into a normal group, a model group, and an electroacupuncture group (electroacupuncture at Huantiao acupoint and Biguan acupoint), with 6 rabbits in each group. The levels of HIF-1α, VEGF, BMP2, and Osterix in rabbit serum were detected by qRT-PCR kit. Immunofluorescence staining was used to detect the expression of H-type vascular marker CD31 and bone destruction-related factor MMP13. Results: The concentrations of HIF-1α, VEGF, BMP2, and Osterix in the model group were significantly lower than those in the normal group (P α, BMP2, Osterix, and CD31, thereby causing bone destruction and vascular destruction, leading to femoral head necrosis. Electroacupuncture can regulate angiogenesis-related factors VEGF, HIF-α, osteogenic-related factors BMP2, Osterix, and H-type vascular marker CD31, increasing the concentrations of VEGF, HIF-α, BMP2, Osterix, and CD31, promoting angiogenesis in the femoral head, and reducing the expression of bone destruction-related factor MMP13, thereby enhancing the self-repair ability of bone tissue. This may be one of the mechanisms of electroacupuncture treatment of steroid-induced femoral head necrosis.
文摘目的该研究利用叶酸拮抗剂甲氨喋呤（MTX）构建叶酸生物学活性受抑制的斑马鱼模型后，观察叶酸生物学活性受抑后对斑马鱼心脏发育的干扰作用以及对斑马鱼心脏发育相关基因BMP2b及HAS2表达的影响。方法用不同浓度的MTX处理不同发育时段的斑马鱼胚胎，于48hpf（hours post fertilization）观察胚胎心脏发育情况并计数各组心脏发育异常个体的百分比及心率，评定MTX对斑马鱼心脏发育的影响程度。用1．5×10^-3M的MTX处理6～10hpf发育时段的斑马鱼胚胎作为MTX处理组。于24hpf及48hpf在显微镜下观察MTX处理组斑马鱼胚胎心脏发育情况。借助胚胎整体原位杂交和Real-time PCR的方法检测BMP2b和HAS2在正常对照组及MTX处理组胚胎的表达水平。结果胚胎早期发育阶段6～12hpf是斑马鱼胚胎对MTX的敏感时期。显微镜下观察结果显示MTX处理组斑马鱼心脏发育延迟，并有心脏形态发育明显异常。胚胎整体原位杂交结果显示MTX处理组斑马鱼心脏发育相关基因BMP2b及HAS2在心脏的表达于36hpf及48hpf下调。Real-time PCR结果显示MTX处理组斑马鱼BMP2b的相对表达量在12，24，36及48hpf减少，HAS2的相对表达量在24，36及48hpf减少。结论叶酸生物学活性受抑对早期胚胎的心脏发育影响较大，可导致斑马鱼心脏发育延迟及心脏形态异常，并下调斑马鱼心脏发育相关基因BMP2b及HAS2的表达，这可能是叶酸生物学活性受抑后导致心脏发育异常的机制之一。
