GAVAC (Heber Biotec S.A, Havana, Cuba) is a commercially available vaccine developed with the Rhipicephalus (Boophilus) microplus Bm86 recombinant antigen. Bm86 is a “concealed” antigen that is present in the plasma...GAVAC (Heber Biotec S.A, Havana, Cuba) is a commercially available vaccine developed with the Rhipicephalus (Boophilus) microplus Bm86 recombinant antigen. Bm86 is a “concealed” antigen that is present in the plasmatic membrane of tick gut epithelial cells with unknown function so far. It is well known that after vaccination in the last fifteen years in Cuba, there was a significant decrease of babesiosis (Babesia bovis and Babesia bigemina) and anaplasmosis (Anaplasma marginale) in cattle. A reduced transmission capacity of ticks fed on tick-immune animals and humans has been reported for several tick-borne pathogens. Recent experiments have demonstrated that an anti-tick vaccine may contribute to the control of tick-borne pathogens not only by decreasing the exposure of susceptible hosts to ticks, but also by reducing the vector capacity of ticks. In this study, the potential of Bm86 vaccination to interfere with pathogen transmission among ticks was evaluated by using as experimental model the brown dog tick Rhipicephalus sanguineus and the tickborne Babesia canis and Ehrlichia canis pathogens. Dogs, vaccinated and not vaccinated, were infested with pathogen-infected ticks and noninfected nymphs of R. sanguineus. After feeding, the pathogen transmission to newly molted adults from co-feeding uninfected nymphs was studied by conventional PCR and qPCR. Results suggest that the anti-Bm86 antibodies could be able to block the transmission of B. canis and/or E. canis from infected to non-infected ticks.展开更多
Naturally occurring polyamines in plants have been implicated in floral initiation, and fruit development. The plant growth regulator BM86 was formulated to stimulate seed production and fruit growth by enhancing poly...Naturally occurring polyamines in plants have been implicated in floral initiation, and fruit development. The plant growth regulator BM86 was formulated to stimulate seed production and fruit growth by enhancing polyamine synthe-sis. The objectives of this study were to determine the effect of BM86 on free polyamine content in cotton (Gos-sypium hirsutum L.) ovaries and on seed set efficiency, and also to investigate genotypic differences in ovarian polyamine content. BM86 was applied at the first flower stage and two weeks later at 2.34 mL/ha. This study showed that application of BM86 had a significant positive effect on ovarian polyamine content of cotton. Putrescine and sper-midine one week after the 1st BM86 application and putrescine two week after the 1st BM86 application, were significantly increased compared to the untreated control. Higher seed set efficiency with the BM86 application was observed when the total number of seeds was used for the calculation. However, when the number of harvestable seeds was used to calculate seed set efficiency BM86 had no significant effect. In addition, application of BM86 did not significantly alter seedcotton yield of the crop. Only small differences in ovarian polyamine content were detected among the genotypes tested, possibly due to the narrow genetic pool of the commercial cotton genotypes used. Application of BM86 can significantly increase cotton seed number by enhancing polyamines biosynthesis, but further research is needed to determine how to capitalize on the increased potential number of harvestable seeds.展开更多
文摘GAVAC (Heber Biotec S.A, Havana, Cuba) is a commercially available vaccine developed with the Rhipicephalus (Boophilus) microplus Bm86 recombinant antigen. Bm86 is a “concealed” antigen that is present in the plasmatic membrane of tick gut epithelial cells with unknown function so far. It is well known that after vaccination in the last fifteen years in Cuba, there was a significant decrease of babesiosis (Babesia bovis and Babesia bigemina) and anaplasmosis (Anaplasma marginale) in cattle. A reduced transmission capacity of ticks fed on tick-immune animals and humans has been reported for several tick-borne pathogens. Recent experiments have demonstrated that an anti-tick vaccine may contribute to the control of tick-borne pathogens not only by decreasing the exposure of susceptible hosts to ticks, but also by reducing the vector capacity of ticks. In this study, the potential of Bm86 vaccination to interfere with pathogen transmission among ticks was evaluated by using as experimental model the brown dog tick Rhipicephalus sanguineus and the tickborne Babesia canis and Ehrlichia canis pathogens. Dogs, vaccinated and not vaccinated, were infested with pathogen-infected ticks and noninfected nymphs of R. sanguineus. After feeding, the pathogen transmission to newly molted adults from co-feeding uninfected nymphs was studied by conventional PCR and qPCR. Results suggest that the anti-Bm86 antibodies could be able to block the transmission of B. canis and/or E. canis from infected to non-infected ticks.
文摘Naturally occurring polyamines in plants have been implicated in floral initiation, and fruit development. The plant growth regulator BM86 was formulated to stimulate seed production and fruit growth by enhancing polyamine synthe-sis. The objectives of this study were to determine the effect of BM86 on free polyamine content in cotton (Gos-sypium hirsutum L.) ovaries and on seed set efficiency, and also to investigate genotypic differences in ovarian polyamine content. BM86 was applied at the first flower stage and two weeks later at 2.34 mL/ha. This study showed that application of BM86 had a significant positive effect on ovarian polyamine content of cotton. Putrescine and sper-midine one week after the 1st BM86 application and putrescine two week after the 1st BM86 application, were significantly increased compared to the untreated control. Higher seed set efficiency with the BM86 application was observed when the total number of seeds was used for the calculation. However, when the number of harvestable seeds was used to calculate seed set efficiency BM86 had no significant effect. In addition, application of BM86 did not significantly alter seedcotton yield of the crop. Only small differences in ovarian polyamine content were detected among the genotypes tested, possibly due to the narrow genetic pool of the commercial cotton genotypes used. Application of BM86 can significantly increase cotton seed number by enhancing polyamines biosynthesis, but further research is needed to determine how to capitalize on the increased potential number of harvestable seeds.
