Background:Diabetes retinopathy(DR)is a complication of diabetes that affects patients’vision.Previous studies have found blueberry anthocyanins extract(BAE)can inhibit the progression of DR,but its mechanism is not ...Background:Diabetes retinopathy(DR)is a complication of diabetes that affects patients’vision.Previous studies have found blueberry anthocyanins extract(BAE)can inhibit the progression of DR,but its mechanism is not completely clear.Methods:To study the role of BAE in diabetes retinopathy,we treated human retinal endothelial cells(HRCECs)with 30 mM high glucose to simulate the microenvironment of diabetes retinopathy and used BAE to intervene the in vitro high glucose-induced retinopathy model.HRCEC cell viability and apoptosis rates were examined by Cell Counting Kit 8(CCK-8)assay and flow cytometry assay.The binding sites between miR-33 and glucocorticoid-induced transcript 1(GLCCI1)were assessed by luciferase reporter assay.Retinal neovascularization and oxidative stress contribute to diabetic retinopathy.The tubule formation assay was applied to detect the retinal neovascularization.The oxidative stress in the HRCECs was manifested by the reactive oxygen species(ROS)level,the malondialdehyde(MDA)level,and the superoxide dismutase(SOD)activity.Results:Compared with HRCECs cells cultured under normal conditions,high glucose(HG)can induce oxidative stress in HRCRCs,specifically manifested in the increase of ROS and MDA levels,and the decrease of SOD activity.BAE relieved the tubule formation in n the HRCEC.BAE also relieved the ROS and MDA levels and increased the SOD activity.Luciferase reporter assay revealed that GLCCI1 is a target molecule downstream of miR-33.In HRCEC,BAE significantly inhibited the expression of miR-33 induced by HG.miR-33 mimic inhibited the BAE’s effects on oxidative stress and angiogenesis in an in vitro high glucose-induced retinopathy model.Conclusion:BAE alleviated the oxidative stress and microangiogenesis of HRCEC by regulating the miR-33/GLCCI1 axis.展开更多
Blueberries are a rich source of anthocyanins, which are associated with health benefits contributing to a reduced risk for many diseases. The present study identified the functional Gardenblue blueberry （Vaccinium a...Blueberries are a rich source of anthocyanins, which are associated with health benefits contributing to a reduced risk for many diseases. The present study identified the functional Gardenblue blueberry （Vaccinium ashei Reade） anthocyanin extracts （GBBAEs） and evaluated their capacity and underlying mechanisms in protecting murine RAW 264.7 cells from lipopolysaccharide （LPS）-stimulated inflammation in vitro. Enzyme-linked immunosorbent assay （ELISA） kit results showed that GBBAEs significantly inhibited the production of nitric oxide （NO）, prostaglandin E2 （PGE2）, interleukin-6 （IL-6）, IL-113, and interferon-y （INF-y）. Real-time polymerase chain reaction （PCR） analysis in- dicated that the mRNA expression levels of IL-6, IL-113, tumor necrosis factor-α （TNF-α）, monocyte chemoattractant protein-1 （MCP-1）, and cyclooxygenase 2 （COX-2） were suppressed in LPS-stimulated RAW 264.7 cells. Additionally, Western blot analysis was used to evaluate the relative protein expression levels of COX-2 and nuclear factor-κB p65 （NF-κBp65）. All these results suggested the potential use of GBBAEs as a functional food for the treatment of in- flammatory diseases.展开更多
The protective effects of blueberry anthocyanin extracts against damage induced by extremely lowfrequency electromagnetic field(ELF-EMF)were investigated in a rat model.Wistar rats were exposed to ELF-EMF with or with...The protective effects of blueberry anthocyanin extracts against damage induced by extremely lowfrequency electromagnetic field(ELF-EMF)were investigated in a rat model.Wistar rats were exposed to ELF-EMF with or without the administration of blueberry anthocyanin extracts(50,100,and 200 mg/kg per day intragastrically once a day)for 30 days.Blueberry anthocyanin extracts supplementation inhibited the decrease in Nissl substance levels,cell membrane integrity,and mitochondrial membrane potential induced by ELF-EMF;prevented the increase in nitric oxide,malondialdehyde,and Ca2+concentrations;suppressed superoxide dismutase and glutathione depletion;and enhanced the cognitive ability of the rats exposed to ELF-EMF.The protective effects of blueberry anthocyanin extracts against hippocampal neuron injury caused by ELF-EMF were dose-dependent.These results demonstrated that blueberry anthocyanin extracts suppress hippocampal neuron injury caused by ELF-EMF by inhibiting cell membrane damage and oxidative stress pathways,and suggested that blueberry anthocyanin treatment potentially prevents hippocampal neuron injury.展开更多
Oleogels,substances that have a liquid phase of continuous oil,are being explored as a potential substitute for trans fatty acids and saturated fatty acids in food.To develop more stable and nutritious alternative lip...Oleogels,substances that have a liquid phase of continuous oil,are being explored as a potential substitute for trans fatty acids and saturated fatty acids in food.To develop more stable and nutritious alternative lipids using blueberry extract,oleogels loaded with resveratrol(R)and blueberry extract were prepared using soybean oil(SO)and peanut oil(PO)as base oils,andβ-sitosterol and lecithin as low-molecular-weight complex oleogelators.Microstructural characterization of oleogels showed that R-loaded and R-enriched blueberry extracts maintained the degree of cross-linking between oleogel crystal units to a certain extent as well as maintained or improved the mouthfeel and hardness of the oleogels.Differential scanning calorimetry and rheometric results demonstrated that the R-loaded ternary oleogels had good thermal stability and strength.With prolonged storage,the physical stability of peanut oil oleogels(POO)was better than that of soybean oil oleogels(SOO).In addition,in vitro simulation experiments revealed that the oleogels could better serve as a carrier for R but could inhibit their bioavailability in R-loaded oleogels.展开更多
基金supported by the Science and Technology Project of Jiangxi Provincial Administration of Traditional Chinese Medicine(Grant Number:2022A359).
文摘Background:Diabetes retinopathy(DR)is a complication of diabetes that affects patients’vision.Previous studies have found blueberry anthocyanins extract(BAE)can inhibit the progression of DR,but its mechanism is not completely clear.Methods:To study the role of BAE in diabetes retinopathy,we treated human retinal endothelial cells(HRCECs)with 30 mM high glucose to simulate the microenvironment of diabetes retinopathy and used BAE to intervene the in vitro high glucose-induced retinopathy model.HRCEC cell viability and apoptosis rates were examined by Cell Counting Kit 8(CCK-8)assay and flow cytometry assay.The binding sites between miR-33 and glucocorticoid-induced transcript 1(GLCCI1)were assessed by luciferase reporter assay.Retinal neovascularization and oxidative stress contribute to diabetic retinopathy.The tubule formation assay was applied to detect the retinal neovascularization.The oxidative stress in the HRCECs was manifested by the reactive oxygen species(ROS)level,the malondialdehyde(MDA)level,and the superoxide dismutase(SOD)activity.Results:Compared with HRCECs cells cultured under normal conditions,high glucose(HG)can induce oxidative stress in HRCRCs,specifically manifested in the increase of ROS and MDA levels,and the decrease of SOD activity.BAE relieved the tubule formation in n the HRCEC.BAE also relieved the ROS and MDA levels and increased the SOD activity.Luciferase reporter assay revealed that GLCCI1 is a target molecule downstream of miR-33.In HRCEC,BAE significantly inhibited the expression of miR-33 induced by HG.miR-33 mimic inhibited the BAE’s effects on oxidative stress and angiogenesis in an in vitro high glucose-induced retinopathy model.Conclusion:BAE alleviated the oxidative stress and microangiogenesis of HRCEC by regulating the miR-33/GLCCI1 axis.
基金Project supported by the Fundamental Research Funds for the Central Universities of China(No.2013PY095)the Clinical Research Project of Health and Family Planning Commission of Wuhan Municipality(No.WX13A05)+1 种基金the Research Project of Wuhan City Central Hospital(No.YQ15A04)the Grant from Key Laboratory of Biological Targeted Therapy of Hubei Province of China(No.02.03.2014-10)
文摘Blueberries are a rich source of anthocyanins, which are associated with health benefits contributing to a reduced risk for many diseases. The present study identified the functional Gardenblue blueberry （Vaccinium ashei Reade） anthocyanin extracts （GBBAEs） and evaluated their capacity and underlying mechanisms in protecting murine RAW 264.7 cells from lipopolysaccharide （LPS）-stimulated inflammation in vitro. Enzyme-linked immunosorbent assay （ELISA） kit results showed that GBBAEs significantly inhibited the production of nitric oxide （NO）, prostaglandin E2 （PGE2）, interleukin-6 （IL-6）, IL-113, and interferon-y （INF-y）. Real-time polymerase chain reaction （PCR） analysis in- dicated that the mRNA expression levels of IL-6, IL-113, tumor necrosis factor-α （TNF-α）, monocyte chemoattractant protein-1 （MCP-1）, and cyclooxygenase 2 （COX-2） were suppressed in LPS-stimulated RAW 264.7 cells. Additionally, Western blot analysis was used to evaluate the relative protein expression levels of COX-2 and nuclear factor-κB p65 （NF-κBp65）. All these results suggested the potential use of GBBAEs as a functional food for the treatment of in- flammatory diseases.
基金supported by the Natural Science Foundation Key Program of Liaoning Province(20170540803)the Liaoning Provincial Department of Education Project(LSNJC201911).
文摘The protective effects of blueberry anthocyanin extracts against damage induced by extremely lowfrequency electromagnetic field(ELF-EMF)were investigated in a rat model.Wistar rats were exposed to ELF-EMF with or without the administration of blueberry anthocyanin extracts(50,100,and 200 mg/kg per day intragastrically once a day)for 30 days.Blueberry anthocyanin extracts supplementation inhibited the decrease in Nissl substance levels,cell membrane integrity,and mitochondrial membrane potential induced by ELF-EMF;prevented the increase in nitric oxide,malondialdehyde,and Ca2+concentrations;suppressed superoxide dismutase and glutathione depletion;and enhanced the cognitive ability of the rats exposed to ELF-EMF.The protective effects of blueberry anthocyanin extracts against hippocampal neuron injury caused by ELF-EMF were dose-dependent.These results demonstrated that blueberry anthocyanin extracts suppress hippocampal neuron injury caused by ELF-EMF by inhibiting cell membrane damage and oxidative stress pathways,and suggested that blueberry anthocyanin treatment potentially prevents hippocampal neuron injury.
基金financed by the 111 project(Project No.D20034)the National Natural Science Fund of China(Project Nos.31771895,31471677)the support for equipment provided by the Key Research and Development Program of Guangdong Province(Project No.2019B020212001).
文摘Oleogels,substances that have a liquid phase of continuous oil,are being explored as a potential substitute for trans fatty acids and saturated fatty acids in food.To develop more stable and nutritious alternative lipids using blueberry extract,oleogels loaded with resveratrol(R)and blueberry extract were prepared using soybean oil(SO)and peanut oil(PO)as base oils,andβ-sitosterol and lecithin as low-molecular-weight complex oleogelators.Microstructural characterization of oleogels showed that R-loaded and R-enriched blueberry extracts maintained the degree of cross-linking between oleogel crystal units to a certain extent as well as maintained or improved the mouthfeel and hardness of the oleogels.Differential scanning calorimetry and rheometric results demonstrated that the R-loaded ternary oleogels had good thermal stability and strength.With prolonged storage,the physical stability of peanut oil oleogels(POO)was better than that of soybean oil oleogels(SOO).In addition,in vitro simulation experiments revealed that the oleogels could better serve as a carrier for R but could inhibit their bioavailability in R-loaded oleogels.
