Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Method...Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Methods:In this study,30 patients who visited the hospital in October 2023 were randomly selected and divided into two groups(15 cases in each group).The same collection method was used for routine blood tests.The tests were performed after anticoagulation and standing for 5 minutes,1 hour,and 5 minutes,2 hours respectively,and the routine blood test indicators at different time points were compared.Results:After comparison,there were no significant differences in the results of routine blood tests at 5 minutes after mixing,anticoagulating,and standing peripheral blood and those at 1 hour and 2 hours(P>0.05).Conclusion:The results of peripheral blood after mixing,anticoagulating,and standing for 5 minutes are stable compared with those after standing for 1 hour and 2 hours.In actual work,the pre-test turnaround time can be appropriately extended.展开更多
[Objectives]The effects of condensed quebracho tannins on the production performance and blood routine indices of Simmental fattening cattle were investigated experimentally.[Methods]Twenty four Simmental fattening ca...[Objectives]The effects of condensed quebracho tannins on the production performance and blood routine indices of Simmental fattening cattle were investigated experimentally.[Methods]Twenty four Simmental fattening cattle were selected and randomly divided into two treatment groups according to their body weight,with 12 animals in each group.The diet of the control group consisted of beef cattle concentrate supplement+silage+hay,and the diet of the experimental group consisted of beef cattle concentrate supplement+silage+hay+tannins,and the experimental period was 30 d.[Results]Compared with the control group,the addition of tannins[30 g/(cattle·d)]to the diet of beef cattle increased the daily weight gain of Simmental beef cattle by 24.68%(P<0.01),while the feed conversion ratio decreased by 2.57%(P>0.05).The gross profit per cattle increased by 973.63 yuan,and the economic benefit increased by 114.78%.Platelets were higher in the experimental group than in the control group(P<0.01);hemoglobin was lower in the experimental group than in the control group(P<0.01);leukocytes,neutrophils,monocytes and lymphocytes were lower in the experimental group than in the control group(P<0.01);and erythrocytes were lower in the experimental group than in the control group(P<0.05).[Conclusions]This study provides a theoretical basis for the rational utilization of quebracho tannins.展开更多
Objective: To explore the clinical effect of blood routine and C-reactive protein to identify bacterial and viral infections in children). Methods: select our pediatric admitted during February 2019-February 2020,100 ...Objective: To explore the clinical effect of blood routine and C-reactive protein to identify bacterial and viral infections in children). Methods: select our pediatric admitted during February 2019-February 2020,100 cases of children as a research object, including 50 cases of virus infection for the control group, the other 50 cases of bacterial infection for research group, for the 100 cases of children with blood routine and C-reactive protein combined test, and the virus and bacterial infection of WBC and C-reactive protein levels. Results: (16.98 ± 4.11) 109 / L, C-reactive protein was (39.64 ± 11.21) ml / L, (6.51 ± 1.86) 109 / L and (5.95 ± 1.85) ml / L, the positive rate was 64.0%, 86.0%, 0.0% and 6.0%, respectively in the control group (P <0.05), statistically significant. Conclusion: In the clinical examination of bacterial and viral infection in children, if the combination of blood routine and C-reactive protein is used, the accuracy rate of diagnosis of children can be improved, which is suitable for its application in pediatric clinic.展开更多
Objective:To investigate and analyze the annual physical examination results of retired employees from a unit in the civil aviation system,focusing on blood lipids,blood glucose,blood uric acid,and blood routine resul...Objective:To investigate and analyze the annual physical examination results of retired employees from a unit in the civil aviation system,focusing on blood lipids,blood glucose,blood uric acid,and blood routine results.The study aims to provide relevant references for formulating reasonable disease management measures for preventing and controlling hyperlipidemia,hyperuricemia,and other conditions in retired employees.Methods:The examination results of 231 participants were collected and analyzed.The participants were divided into four groups based on age:middle-aged group,young-old group,middle-old group,and old-old group.The blood test results were compared across these groups,and an assessment of atherosclerotic cardiovascular disease(ASCVD)risk levels was completed in conjunction with medical history.Blood test results were also compared by gender.Results:There were no significant statistical differences in blood test results when grouped by age.However,the prevalence of hyperuricemia was higher in males than in females,while the prevalence of hypercholesterolemia was higher in females than in males.The LDL-C target achievement rate was lower in the moderate-and-high-risk group as well as the very high-risk group as defined by ASCVD risk levels.Conclusion:Management of hyperuricemia and hyperlipidemia in retired employees(elderly patients)should be strengthened to reduce the risk of ASCVD events and alleviate the potential medical burden associated with disease progression.展开更多
Objective: to study and analyze the application value of blood routine in the diagnosis of upper respiratory tract infection. Methods: into our hospital from February 2021 to January 2022 admitted 60 cases of suspecte...Objective: to study and analyze the application value of blood routine in the diagnosis of upper respiratory tract infection. Methods: into our hospital from February 2021 to January 2022 admitted 60 cases of suspected upper respiratory tract infection, collect peripheral blood after admission blood routine examination, and refer to clinical comprehensive diagnosis on the diagnostic efficiency of blood routine examination evaluation analysis, and this group of suspected upper respiratory tract infection blood routine examination qualitative and quantitative results analysis. Results: this group of 60 cases of suspected upper respiratory tract infection cases, the clinical comprehensive diagnosis of upper respiratory tract infection in 45 cases, qualitative analysis of blood routine examination results for 43 cases, referring to clinical comprehensive diagnosis analysis, this group of 60 cases of suspected upper respiratory tract infection cases of blood routine examination, diagnosis compliance rate, specificity, sensitivity are higher, diagnostic efficiency is outstanding. Among the 43 cases with routine blood examination results being positive in this group of suspected upper respiratory tract infection cases, the quantitative results of quantitative white blood cell count were higher than the 17 cases with negative results, which was statistically significant (P <0.05). Conclusion: the quantitative analysis and qualitative analysis of blood routine white blood cell count in the diagnosis of upper respiratory tract infection can provide a practical basis for the diagnosis and differentiation of upper respiratory tract infection, and the diagnostic efficiency is outstanding.展开更多
To study and analyze the influence of peripheral blood and venous blood on the test results in blood routine test. Methods: from September 2020 to June 2021, 152 patients who underwent blood routine test were included...To study and analyze the influence of peripheral blood and venous blood on the test results in blood routine test. Methods: from September 2020 to June 2021, 152 patients who underwent blood routine test were included in the laboratory department of our hospital. All patients collected peripheral blood and venous blood for blood routine test respectively. The blood source of peripheral blood for blood routine test was peripheral blood group, and the blood source of elbow vein blood for blood routine test was venous blood group, The differences of blood routine test results between the two groups at 0 min were compared, and the blood sources of the two groups were tested for many times at 0 min, 10 min and 30 min after blood collection. The test results were analyzed and compared, and the satisfaction of the two blood collection methods, time and pain were evaluated. Results: at 0 min of blood collection, the leukocyte count value of venous blood group was lower than that of peripheral blood group, while the erythrocyte count value, hemoglobin concentration value, platelet count value, erythrocyte backlog value and average hemoglobin concentration value of venous blood group were higher than those of peripheral blood group (P < 0.05);However, there was no significant difference between the two groups (P > 0.05);The test results of peripheral blood group and venous blood group were compared for multiple tests at 0 min, 10 min and 30 min after blood collection respectively. There was no significant difference in the test results of erythrocyte count and hemoglobin concentration in peripheral blood group at each time period (P > 0.05), while the test results of leukocyte count and platelet count at 0 min were different from those at 10 min. There was significant difference in the results of 30 min test (P < 0.05), indicating that the index value will change with time;There was no significant difference in the test results of leukocyte count, erythrocyte count, hemoglobin concentration and platelet count in the venous blood group (P > 0.05), indicating that the indexes in the blood were relatively stable;Comparing the satisfaction scores of the two groups, the scores of the two groups were higher, and the satisfaction of peripheral blood was slightly higher, but there was no significant difference between the two groups (P > 0.05).Conclusion: comparing the satisfaction of the two groups of patients, the acceptability of the two blood collection methods is very high. From the analysis of the test results, on the whole, the test results of peripheral blood are slightly lower than those of venous blood. It is analyzed that the reason for this result may be that the peripheral blood causes the damage of blood cells during the extrusion process and reduces the count, and the white blood cell count. The platelet count value will change over time and affect the test results, while the venous blood test results have little difference within 30min, indicating that various indexes in venous blood are relatively stable within a certain period of time, and the accuracy of test results is higher.展开更多
Objective: to analyze the diagnostic value of blood routine examination in children with bacterial infection. Methods: the clinical data of children with infectious diseases examined in our hospital were retrospective...Objective: to analyze the diagnostic value of blood routine examination in children with bacterial infection. Methods: the clinical data of children with infectious diseases examined in our hospital were retrospectively analyzed. From March 2019 to March 2020, 45 children with bacterial infection were selected as the bacterial infection group, 45 children with viral infection were selected as the viral infection group, and 50 children who came to our hospital for physical examination at the same time were selected as the control group. All of them received blood routine examination. The WBC count and WBC positive rate of the three groups were compared. Results: WBC of bacterial infection group was (17.97 ± 3.54), significantly higher than that of virus infection group (10.33 ± 3.60) and control group (8.02 ± 2.54) (P < 0.05). There was no significant difference between the virus infection group and the control group (P > 0.05). The positive rate of WBC in bacterial infection group was 80.00%, which was significantly higher than that in virus infection group (42.22%) (P < 0.05). Conclusion: blood routine examination can provide reliable data for clinical diagnosis of bacterial infection in children.展开更多
[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Ta...[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Tan sheep were randomly divided into two treatment groups according to their body weight, 46 in each group. The control check group(CK) was feed with conventional diet, and the experimental group was fed with the addition of antimicrobial peptide on the basis of the conventional diet, with the added amount of 2.5 g/sheep per day. The experimental period was 60 d. [Results] The incidence rate was 75.06% lower in the experimental group than in the CK. The average daily weight gain per sheep was 11.27% higher in the experimental group than in the CK(P<0.05). The feed conversion ratio was 8.45% lower in the experimental group than in the CK(P<0.05). The average daily gross profit per sheep was 12.12% higher in the experimental group than in the CK. For slaughter performance, the data difference of each item was not significant. The PH at 45 min and 24 h after slaughter was within the normal range of fresh mutton. The cooked meat percentage and water loss rate showed no significant differences(P>0.05). The marbling ranged from 2.45 to 2.50, indicating that the fat content was moderate, and the difference between groups was not significant(P>0.05). The flesh color ranged from 3.00 to 3.15, between light red and bright red, belonging to the normal color of mutton, and the difference between groups was not significant(P>0.05). The shear force was between 2.50 and 2.65, without a significant difference between groups(P>0.05). The white blood cells, lymphocytes and platelets in the experimental group were lower than those in the CK(P<0.01). The erythrocytes and hemoglobin in the erythrocyte group were higher than those in the CK(P<0.05). The neutrophils and monocytes in the experimental group were lower than those in the CK(P<0.05). [Conclusions] This study provides a technical basis for the rational use of antimicrobial peptides and their application in ruminants.展开更多
Objective: the differences of blood routine test results between venous blood and capillary blood were analyzed. Methods: The subjects of the study were 120 healthy outpatients from August 2019 to August 2021. They we...Objective: the differences of blood routine test results between venous blood and capillary blood were analyzed. Methods: The subjects of the study were 120 healthy outpatients from August 2019 to August 2021. They were divided into two groups according to the random method (n=60 for each group). The capillary blood was collected in the control group and venous blood was collected in the observation group. The results of WBC, HCT, RBC, Hb, PLT, MCHC, MCV and HCT were statistically compared between the two groups. Results: WBC of the observation group was lower than that of the control group, HCT, RBC, Hb, PLT and MCHC of the observation group were higher than those of the control group, p < 0.05 (the difference was statistically significant). Compared with the control group, the MCV and HCT of the observation group had no significant difference (P>0.05). Conclusion: venous blood collection in blood routine test can improve the accuracy of test results, and can be the preferred collection method in blood collection with high clinical value.展开更多
Objective:Accurate preoperative identification of benign or malignant pancreatic cystic neoplasms(PCN)may help clinicians make better intervention choices and will be essential for individualized treatment.Methods:Pre...Objective:Accurate preoperative identification of benign or malignant pancreatic cystic neoplasms(PCN)may help clinicians make better intervention choices and will be essential for individualized treatment.Methods:Preoperative ultrasound and laboratory examination findings,and demographic characteristics were collected from patients.Multiple logistic regression was used to identify independent risk factors associated with malignant PCN,which were then included in the nomogram and validated with an external cohort.The Net Reclassification Index(NRI)and Integrated Discrimination Improvement(IDI)were calculated to evaluate the improvement in the predictive power of the new model with respect to that of a combined imaging and tumor marker prediction model.Results:Malignant PCN were found in 83(40.7%)and 33(38.7%)of the model and validation cohorts,respectively.Multivariate analysis identified age,tumor location,imaging of tumor boundary,blood type,mean hemoglobin concentration,neutrophil-tolymphocyte ratio,carbohydrate antigen 19-9,and carcinoembryonic antigen as independent risk factors for malignant PCN.The calibration curve indicated that the predictions based on the nomogram were in excellent agreement with the actual observations.A nomogram score cutoff of 192.5 classified patients as having low vs.high risk of malignant PCN.The model achieved good C-statistics of 0.929(95%CI 0.890–0.968,P<0.05)and 0.951(95%CI 0.903–0.998,P<0.05)in predicting malignancy in the development and validation cohorts,respectively.NRI=0.268;IDI=0.271(P<0.001 for improvement).The DCA curve indicated that our model yielded greater clinical benefits than the comparator model.Conclusions:The nomogram showed excellent performance in predicting malignant PCN and may help surgeons select patients for detailed examination and surgery.The nomogram is freely available at https://wangjunjinnomogram.shinyapps.io/DynNomapp/.展开更多
[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,t...[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs（ 22 males and 22 females） once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count（ RBC） and hemoglobin count（ HGB） both reached the lowest levels（ 6. 85 × 10^12/L and 97. 1 g/L）at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age（ P 〈 0. 05,similarly hereinafter）; mean corpuscular hemoglobin（ MCH） and mean corpuscular hemoglobin concentration（ MCHC） demonstrated basically the same variation pattern,which both reached the lowest levels（ 15. 11 pg and 275. 70 g/L） at 70 days of age and reached the highest levels（ 16. 40 pg and 314. 30 g/L） at 91 days of age; hematocrit（ HCT）,mean corpuscular volume（ MCV） and red blood cell distribution width（ RDW） exhibited relatively stable changes with no significant differences among different day ages（ P 〉 0. 05,similarly hereinafter）. Among white blood cell indexes of Guike pig at different day ages,white blood cell count（ WBC） reached the lowest level（ 13. 71 × 10^9/L） at 63 days of age and reached the highest level（ 17. 52 × 10^9/L） at 84 days of age,with significant differences; neutrophil count（ NEUT）reached the lowest level（ 3. 61 × 10^9/L） at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count（ LYMPH） exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count（ PLT）,thrombocytocrit（ PCT） and platelet volume distribution width（ PDW） exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume（ MPV）,PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age.展开更多
Objective:To study the levels of serum cystatin C(Cys-C),total bile acid(TBA),and other routine blood parameters on patients with decompensated hepatitis B cirrhosis.Methods:Study group 1 consisted of 30 patients with...Objective:To study the levels of serum cystatin C(Cys-C),total bile acid(TBA),and other routine blood parameters on patients with decompensated hepatitis B cirrhosis.Methods:Study group 1 consisted of 30 patients with hepatitis B-related decompensated cirrhosis,and study group 2 consisted of 30 patients with hepatitis B;while the control group consisted of 30 healthy people who underwent physical examination.The blood parameters were used to evaluate the clinical treatment effect of patients.Results:The TBA,Cys-C,alanine transaminase(ALT),total bilirubin(TBIL),aspartate aminotransferase(AST),and international normalized ratio(INR)in study group 1 were significantly higher than those of study group 2 and the control group;while the platelet count(PLT),hemoglobin(Hb),albumin(ALB),and estimated glomerular filtration rate(eGFR)were significantly lower in the study group 1 compared to the control group and study group 2(P<0.05).The Cys-C,PLT,TBA,AST,TBIL,and INR of patients in study group 1 who were successfully treated were significantly lower than the patients who were not successfully treated(P<0.05).Conclusion:Serum Cys-C,TBA,and routine blood parameters are useful in predicting the condition and the prognosis of patients of hepatitis B-related decompensated cirrhosis.展开更多
Objective: to discuss the clinical value of new hemolysin and traditional hemolysin in blood routine examination. Methods: a total of 89 blood routine test subjects were selected from our hospital. The average control...Objective: to discuss the clinical value of new hemolysin and traditional hemolysin in blood routine examination. Methods: a total of 89 blood routine test subjects were selected from our hospital. The average control group (45 cases, using traditional hemolysin) and the study group (44 cases, using new hemolysin) were compared. Results: there were no statistically significant differences in the levels of red blood cells, white blood cells and platelets between the two groups (P > 0.05). The level of hemoglobin and satisfaction score in the study group were significantly higher than those in the control group (P < 0.05). The test time and cost in the study group were significantly lower than those in the control group (P < 0.05). Conclusion: the new hemolysin has more application value in routine blood test.展开更多
Objective: this study focuses on the main causes and solutions of the routine blood test. Methods: in this study, 120 patients with the error in the routine blood test results from March 2020 to March 2021 were random...Objective: this study focuses on the main causes and solutions of the routine blood test. Methods: in this study, 120 patients with the error in the routine blood test results from March 2020 to March 2021 were randomly selected as the study subjects, and the clinical data of all the study subjects were analyzed retrospectively. All patients received 2mL blood collection from peripheral blood and 14ml of venous blood. The venous blood samples were divided into 7 groups, among which group 1 was tested immediately after collection, groups 2 and 3 were stored in refrigerator (4℃) and room temperature, groups 4 and 4 hours respectively, group 6 and 7 were treated with 1.6 mg/ml and 2.8 mg/ml anticoagulant respectively. Peripheral blood needs to be tested immediately. The root causes of common errors are summarized, and the influence of different blood collection location, placement time and anticoagulant concentration on the routine blood test indicators is discussed, and the corresponding solutions are summarized. Results: the results of this study showed that platelet content in peripheral blood samples was significantly lower than venous blood, with significantly different data between groups (P <0.05). Blood samples were retained for 2 hours after collection, the hemoglobin concentration and platelet content were lower than the 4-hour retained samples, and the data differences between groups were significant (P <0.05).Blood samples were stored in the refrigerator, the hemoglobin concentration was lower than those stored at room temperature, and there were significant differences between groups (P <0.05).After the blood samples were treated with 2.8 mg/ml anticoagulant concentration, the content of each index was significantly higher than that treated with 1.6 mg/ml, and the data between the two groups varied significantly (P <0.05).Among the routine blood tests, 120 subjects had the error in the routine blood test indicators, including the unreasonable anticoagulant concentration, long inspection time, unreasonable storage and personal factors of the subjects. Conclusion: in the routine blood test, many factors resulted in the test error. The hospital should clarify the main influencing factors, improve the efficiency and quality of routine blood test by strengthening the training of relevant personnel, actively communicating with the subjects, and making reasonable use of anticoagulant, reducing the probability of error, and provide reliable support for the clinical diagnosis and treatment of doctors.展开更多
The influence of exercise at high temperature on adult males＇ routine blood indexes and biochemical indexes and the expression of HSP72 in peripheral blood lymphocytes （PBLs） was studied in order to provide theoret...The influence of exercise at high temperature on adult males＇ routine blood indexes and biochemical indexes and the expression of HSP72 in peripheral blood lymphocytes （PBLs） was studied in order to provide theoretical ground for health supervision of adults receiving exercise at high temperature. 180 adult males were selected and divided into exercise group and control group, in which the exercise group was subdivided into subgroup 1 and subgroup 2 receiving exercise at high temperature in the afternoon and in the morning, respectively. Peripheral venous blood was phle- botomized before and after the exercise to examine routine blood indexes and blood biochemical indexes. The expression levels of HSP72 in PBLs were detected by flow cytometry. The results showed that the routine blood indexes and biochemical indexes in each group were within the range of normal values of male adults. There was no significant difference between each exercise group and control group in indexes before exercise. After exercise, the expression levels of HSP72 in PBLs in exercise groups were higher than those before exercise, and HSP72 expression levels in subgroup 1 were obviously higher than those in subgroup 2 and control group. The contents of ALT, urea, Na＋, Cl-, Ca2＋ and K＋ in subgroups 1 and 2 were lower than those in control group, but CK level was higher than in control group （P〈0.05）. The contents of Na＋ and Clin subgroup 1 were relatively lower than those in subgroup 2 （P〈0.05）. It was concluded that while receiving exercise at high temperature, adult males＇ HSP72 levels in PBLs could be increased and the biochemical indexes changed. Attention should be paid to health supervision to avoid obvious body injuries at high temperature.展开更多
Objective:To study the effect of training under high temperature on blood and heat shock protein 72(HSP72)expression of flying saucer athletes.Methods:The numbers of training group and control group are 30.Twenty-four...Objective:To study the effect of training under high temperature on blood and heat shock protein 72(HSP72)expression of flying saucer athletes.Methods:The numbers of training group and control group are 30.Twenty-four flying saucer athletes in Zhejiang province were selected and randomly divided into training group and control group,15 in each group.Peripheral venous blood of each group was taken before and after the experiment.The HSP72 content of lymphocyte,blood routine and biochemical indexes were measured respectively.Results:There was significant difference in white blood cell in training group count before and after the experiment(P<0.05).The expression of HSP72 and the white blood cell in training group was higher than that control group(P<0.05).Conclusions:Organism could be affected and turned to stress state due to training under high temperature.It is necessary to provide the safeguard for the flying saucer athletes under high temperature in order to prevent the damage.展开更多
To evaluate the subchronic and chronic toxicity of Fuyanxiao capsules,Sprague-Dawley(SD)rats were used in toxicity studies.In the subchronic toxicity study,50 female rats were randomly divided into a high-dose group(5...To evaluate the subchronic and chronic toxicity of Fuyanxiao capsules,Sprague-Dawley(SD)rats were used in toxicity studies.In the subchronic toxicity study,50 female rats were randomly divided into a high-dose group(5.4g/kg/day)and a control group,with 15 rats in each,and medium(2.7g/kg/day)and low(1.35g/kg/day)dose groups,with 10 rats in each.The test substance was administered orally(mixed with feed,twice daily)for 90 consecutive days.In the chronic toxicity study,40 female rats were randomly divided into high,medium,and low dose groups and a control group,with 10 rats in each.The test substance was administered orally in the same manner for 180 consecutive days.Clinical signs,body weight,and food consumption were observed and recorded daily.At the end of the terminal phase(the first 10 rats from each group,1 day after the last dose)and the recovery phase(the last 5 rats from the control group and the high-dose group,observed for an additional 28 days after the last dose),blood and urine samples,as well as organs,were collected.Organ coefficients were calculated,and various hematological and urinary indicators were detected,followed by pathological analysis.The results showed that there were no significant differences in body weight,food consumption,or organ coefficients between any of the dose groups and the control group in both subchronic and chronic toxicity studies(P>0.05).Histopathological examination revealed no lesions,suggesting no tissue or organ damage in any of the dose groups.The rats exhibited good mental status,and hematological and urinary physiological indicators were within normal ranges,indicating stable liver and kidney function,hematopoietic system of the bone marrow,and internal environment in all dose groups.Therefore,Fuyanxiao capsule has no obvious subchronic or chronic toxicity in SD rats,and it is safe and reliable to use at reasonable dosage in clinical practice.展开更多
We here aimed to investigate the impact of gender on the clinical characteristics and laboratory results of patients with coronavirus disease 2019(COVID-19)and provide clues to the pathological mechanisms underlying C...We here aimed to investigate the impact of gender on the clinical characteristics and laboratory results of patients with coronavirus disease 2019(COVID-19)and provide clues to the pathological mechanisms underlying COVID-19.A retrospective study was performed.Clinical characteristics,severity of lung infection,laboratory results,and prognoses of patients of different gender were analyzed.A total of 242 patients were finally included.The median age was 58 years(IQR:40-68),including 54(22.3%)hospital staffs.Ninety-four(38.8%)were male and 148(61.1%)were female.The proportion of patients with diabetes was significantly higher in the male group than in the female group(P=0.034).Male patients had a significantly larger proportion of severe lung infection,higher leukocyte count,neutrophil count,neutrophil-to-lymphocyte ratio,C-reactive protein,and procalcitonin than femalc.Furthermore,male patients had worse liver,cardiac,and coagulation function than their female counterparts.Male patients with COVID-19 showed more severe inflammation reaction and coagulation dysfunction than female patients.In conclusion,gender is associated with host response to SARS-CoV-2 infection.展开更多
Objective:To observe the effects of different hypoxia time on hematology and histomorphology of rats by simulating the environment of 7000m altitude,and to provide the basis for the establishment of acute hypoxia mode...Objective:To observe the effects of different hypoxia time on hematology and histomorphology of rats by simulating the environment of 7000m altitude,and to provide the basis for the establishment of acute hypoxia model.Methods:40 SD rats were randomly divided into 0h hypoxia group,6h,12h,24h,and 36h group.The hypoxia environment at 7000m was simulated by low-pressure oxygen chamber.The serum SOD,MDA,LDH biochemical indexes and the morphological changes of HE staining in heart,lung and brain tissues of rats were detected under different hypoxia time.Results:Compared with hypoxia group for 0h,the number of RBC in acute hypoxia group for 6h decreased and increased with the prolongation of hypoxia time,and the highest number of RBC.The WBC,LYMP,GRAN,HGB,HCT and RCT counts of the acute hypoxia group decreased for 6h,and the above indexes increased with the prolongation of hypoxia time.Compared with the 0h hypoxia group,SOD activity in serum decreased at different hypoxia time,and MDA content in the 24h and 36h hypoxia groups showed a fluctuating change and reached a peak at 12h hypoxia.The activity of LDH fluctuated,and reached the peak at 12hLDH after hypoxia,and then gradually decreased.He staining of the brain tissue of the heart and lung,which was close to normal after hypoxia for 36h,showed that with the prolongation of the hypoxia time,the damage degree of each organ increased continuously.Interstitial pneumonia or perivasculitis occurred in the lung,and perivascular edema and pulmonary interstitial widening were observed.Cardiac tissue showed obvious degeneration and necrosis of cardiomyocytes,lysis and necrosis of local myocardial interstitium and valve fibers.A large number of inflammatory cells could be seen infiltrating into the brain tissue in the CA2 CA3 DG region.The neuron cells were denatuated,the cell volume was reduced,basophilic was enhanced,and the nerve cells were triangular.The cells were pyknotic and basophilic,with a few transverse axis structures.Conclusion:acute low pressure hypoxia can cause red blood cells white blood cells within 6 h number of platelets Hemoglobin concentrations were lower,as after hypoxia prolonged period of count increased serum biochemical index SOD activity were lower,the content of MDA and activity of LDH outer-plane changes Can cause heart lung tissue pathological damage,and increased with prolonged.展开更多
基金Project of Guangdong Provincial Medical Science and Technology Research Fund(A2022011)Major Science and Technology Project of Shenzhen Nanshan District Health System(NSZD2023067)Sub-project of Education(Health)Science and Technology Project of Nanshan District Technology Research and Development and Creative Design Project in Shenzhen(NS2022002)。
文摘Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Methods:In this study,30 patients who visited the hospital in October 2023 were randomly selected and divided into two groups(15 cases in each group).The same collection method was used for routine blood tests.The tests were performed after anticoagulation and standing for 5 minutes,1 hour,and 5 minutes,2 hours respectively,and the routine blood test indicators at different time points were compared.Results:After comparison,there were no significant differences in the results of routine blood tests at 5 minutes after mixing,anticoagulating,and standing peripheral blood and those at 1 hour and 2 hours(P>0.05).Conclusion:The results of peripheral blood after mixing,anticoagulating,and standing for 5 minutes are stable compared with those after standing for 1 hour and 2 hours.In actual work,the pre-test turnaround time can be appropriately extended.
基金Supported by National Modern Agriculture(Beef Cattle and Yak)Industrial Technology System(CARS-37)Feed Industry Expert Technical Service Team Project of Ningxia Agriculture and Rural Affairs DepartmentAnimal Husbandry New Technology Promotion Project of Ningxia Feed Workstation.
文摘[Objectives]The effects of condensed quebracho tannins on the production performance and blood routine indices of Simmental fattening cattle were investigated experimentally.[Methods]Twenty four Simmental fattening cattle were selected and randomly divided into two treatment groups according to their body weight,with 12 animals in each group.The diet of the control group consisted of beef cattle concentrate supplement+silage+hay,and the diet of the experimental group consisted of beef cattle concentrate supplement+silage+hay+tannins,and the experimental period was 30 d.[Results]Compared with the control group,the addition of tannins[30 g/(cattle·d)]to the diet of beef cattle increased the daily weight gain of Simmental beef cattle by 24.68%(P<0.01),while the feed conversion ratio decreased by 2.57%(P>0.05).The gross profit per cattle increased by 973.63 yuan,and the economic benefit increased by 114.78%.Platelets were higher in the experimental group than in the control group(P<0.01);hemoglobin was lower in the experimental group than in the control group(P<0.01);leukocytes,neutrophils,monocytes and lymphocytes were lower in the experimental group than in the control group(P<0.01);and erythrocytes were lower in the experimental group than in the control group(P<0.05).[Conclusions]This study provides a theoretical basis for the rational utilization of quebracho tannins.
文摘Objective: To explore the clinical effect of blood routine and C-reactive protein to identify bacterial and viral infections in children). Methods: select our pediatric admitted during February 2019-February 2020,100 cases of children as a research object, including 50 cases of virus infection for the control group, the other 50 cases of bacterial infection for research group, for the 100 cases of children with blood routine and C-reactive protein combined test, and the virus and bacterial infection of WBC and C-reactive protein levels. Results: (16.98 ± 4.11) 109 / L, C-reactive protein was (39.64 ± 11.21) ml / L, (6.51 ± 1.86) 109 / L and (5.95 ± 1.85) ml / L, the positive rate was 64.0%, 86.0%, 0.0% and 6.0%, respectively in the control group (P <0.05), statistically significant. Conclusion: In the clinical examination of bacterial and viral infection in children, if the combination of blood routine and C-reactive protein is used, the accuracy rate of diagnosis of children can be improved, which is suitable for its application in pediatric clinic.
文摘Objective:To investigate and analyze the annual physical examination results of retired employees from a unit in the civil aviation system,focusing on blood lipids,blood glucose,blood uric acid,and blood routine results.The study aims to provide relevant references for formulating reasonable disease management measures for preventing and controlling hyperlipidemia,hyperuricemia,and other conditions in retired employees.Methods:The examination results of 231 participants were collected and analyzed.The participants were divided into four groups based on age:middle-aged group,young-old group,middle-old group,and old-old group.The blood test results were compared across these groups,and an assessment of atherosclerotic cardiovascular disease(ASCVD)risk levels was completed in conjunction with medical history.Blood test results were also compared by gender.Results:There were no significant statistical differences in blood test results when grouped by age.However,the prevalence of hyperuricemia was higher in males than in females,while the prevalence of hypercholesterolemia was higher in females than in males.The LDL-C target achievement rate was lower in the moderate-and-high-risk group as well as the very high-risk group as defined by ASCVD risk levels.Conclusion:Management of hyperuricemia and hyperlipidemia in retired employees(elderly patients)should be strengthened to reduce the risk of ASCVD events and alleviate the potential medical burden associated with disease progression.
文摘Objective: to study and analyze the application value of blood routine in the diagnosis of upper respiratory tract infection. Methods: into our hospital from February 2021 to January 2022 admitted 60 cases of suspected upper respiratory tract infection, collect peripheral blood after admission blood routine examination, and refer to clinical comprehensive diagnosis on the diagnostic efficiency of blood routine examination evaluation analysis, and this group of suspected upper respiratory tract infection blood routine examination qualitative and quantitative results analysis. Results: this group of 60 cases of suspected upper respiratory tract infection cases, the clinical comprehensive diagnosis of upper respiratory tract infection in 45 cases, qualitative analysis of blood routine examination results for 43 cases, referring to clinical comprehensive diagnosis analysis, this group of 60 cases of suspected upper respiratory tract infection cases of blood routine examination, diagnosis compliance rate, specificity, sensitivity are higher, diagnostic efficiency is outstanding. Among the 43 cases with routine blood examination results being positive in this group of suspected upper respiratory tract infection cases, the quantitative results of quantitative white blood cell count were higher than the 17 cases with negative results, which was statistically significant (P <0.05). Conclusion: the quantitative analysis and qualitative analysis of blood routine white blood cell count in the diagnosis of upper respiratory tract infection can provide a practical basis for the diagnosis and differentiation of upper respiratory tract infection, and the diagnostic efficiency is outstanding.
文摘To study and analyze the influence of peripheral blood and venous blood on the test results in blood routine test. Methods: from September 2020 to June 2021, 152 patients who underwent blood routine test were included in the laboratory department of our hospital. All patients collected peripheral blood and venous blood for blood routine test respectively. The blood source of peripheral blood for blood routine test was peripheral blood group, and the blood source of elbow vein blood for blood routine test was venous blood group, The differences of blood routine test results between the two groups at 0 min were compared, and the blood sources of the two groups were tested for many times at 0 min, 10 min and 30 min after blood collection. The test results were analyzed and compared, and the satisfaction of the two blood collection methods, time and pain were evaluated. Results: at 0 min of blood collection, the leukocyte count value of venous blood group was lower than that of peripheral blood group, while the erythrocyte count value, hemoglobin concentration value, platelet count value, erythrocyte backlog value and average hemoglobin concentration value of venous blood group were higher than those of peripheral blood group (P < 0.05);However, there was no significant difference between the two groups (P > 0.05);The test results of peripheral blood group and venous blood group were compared for multiple tests at 0 min, 10 min and 30 min after blood collection respectively. There was no significant difference in the test results of erythrocyte count and hemoglobin concentration in peripheral blood group at each time period (P > 0.05), while the test results of leukocyte count and platelet count at 0 min were different from those at 10 min. There was significant difference in the results of 30 min test (P < 0.05), indicating that the index value will change with time;There was no significant difference in the test results of leukocyte count, erythrocyte count, hemoglobin concentration and platelet count in the venous blood group (P > 0.05), indicating that the indexes in the blood were relatively stable;Comparing the satisfaction scores of the two groups, the scores of the two groups were higher, and the satisfaction of peripheral blood was slightly higher, but there was no significant difference between the two groups (P > 0.05).Conclusion: comparing the satisfaction of the two groups of patients, the acceptability of the two blood collection methods is very high. From the analysis of the test results, on the whole, the test results of peripheral blood are slightly lower than those of venous blood. It is analyzed that the reason for this result may be that the peripheral blood causes the damage of blood cells during the extrusion process and reduces the count, and the white blood cell count. The platelet count value will change over time and affect the test results, while the venous blood test results have little difference within 30min, indicating that various indexes in venous blood are relatively stable within a certain period of time, and the accuracy of test results is higher.
文摘Objective: to analyze the diagnostic value of blood routine examination in children with bacterial infection. Methods: the clinical data of children with infectious diseases examined in our hospital were retrospectively analyzed. From March 2019 to March 2020, 45 children with bacterial infection were selected as the bacterial infection group, 45 children with viral infection were selected as the viral infection group, and 50 children who came to our hospital for physical examination at the same time were selected as the control group. All of them received blood routine examination. The WBC count and WBC positive rate of the three groups were compared. Results: WBC of bacterial infection group was (17.97 ± 3.54), significantly higher than that of virus infection group (10.33 ± 3.60) and control group (8.02 ± 2.54) (P < 0.05). There was no significant difference between the virus infection group and the control group (P > 0.05). The positive rate of WBC in bacterial infection group was 80.00%, which was significantly higher than that in virus infection group (42.22%) (P < 0.05). Conclusion: blood routine examination can provide reliable data for clinical diagnosis of bacterial infection in children.
基金Supported by Ningxia Agricultural Reclamation Group Science and Technology Innovation ProjectNew Feed Technology Promotion Project of Ningxia Agriculture and Rural DepartmentNingxia Feed Industry Expert Technical Service Group Project。
文摘[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Tan sheep were randomly divided into two treatment groups according to their body weight, 46 in each group. The control check group(CK) was feed with conventional diet, and the experimental group was fed with the addition of antimicrobial peptide on the basis of the conventional diet, with the added amount of 2.5 g/sheep per day. The experimental period was 60 d. [Results] The incidence rate was 75.06% lower in the experimental group than in the CK. The average daily weight gain per sheep was 11.27% higher in the experimental group than in the CK(P<0.05). The feed conversion ratio was 8.45% lower in the experimental group than in the CK(P<0.05). The average daily gross profit per sheep was 12.12% higher in the experimental group than in the CK. For slaughter performance, the data difference of each item was not significant. The PH at 45 min and 24 h after slaughter was within the normal range of fresh mutton. The cooked meat percentage and water loss rate showed no significant differences(P>0.05). The marbling ranged from 2.45 to 2.50, indicating that the fat content was moderate, and the difference between groups was not significant(P>0.05). The flesh color ranged from 3.00 to 3.15, between light red and bright red, belonging to the normal color of mutton, and the difference between groups was not significant(P>0.05). The shear force was between 2.50 and 2.65, without a significant difference between groups(P>0.05). The white blood cells, lymphocytes and platelets in the experimental group were lower than those in the CK(P<0.01). The erythrocytes and hemoglobin in the erythrocyte group were higher than those in the CK(P<0.05). The neutrophils and monocytes in the experimental group were lower than those in the CK(P<0.05). [Conclusions] This study provides a technical basis for the rational use of antimicrobial peptides and their application in ruminants.
文摘Objective: the differences of blood routine test results between venous blood and capillary blood were analyzed. Methods: The subjects of the study were 120 healthy outpatients from August 2019 to August 2021. They were divided into two groups according to the random method (n=60 for each group). The capillary blood was collected in the control group and venous blood was collected in the observation group. The results of WBC, HCT, RBC, Hb, PLT, MCHC, MCV and HCT were statistically compared between the two groups. Results: WBC of the observation group was lower than that of the control group, HCT, RBC, Hb, PLT and MCHC of the observation group were higher than those of the control group, p < 0.05 (the difference was statistically significant). Compared with the control group, the MCV and HCT of the observation group had no significant difference (P>0.05). Conclusion: venous blood collection in blood routine test can improve the accuracy of test results, and can be the preferred collection method in blood collection with high clinical value.
基金supported by the National Natural Science Foundation of China(Grant Nos.82030092,81720108028,and 81802433)the Key Program of Prevention and Treatment of Chronic Diseases of Tianjin(Grant No.17ZXMFSY00010).
文摘Objective:Accurate preoperative identification of benign or malignant pancreatic cystic neoplasms(PCN)may help clinicians make better intervention choices and will be essential for individualized treatment.Methods:Preoperative ultrasound and laboratory examination findings,and demographic characteristics were collected from patients.Multiple logistic regression was used to identify independent risk factors associated with malignant PCN,which were then included in the nomogram and validated with an external cohort.The Net Reclassification Index(NRI)and Integrated Discrimination Improvement(IDI)were calculated to evaluate the improvement in the predictive power of the new model with respect to that of a combined imaging and tumor marker prediction model.Results:Malignant PCN were found in 83(40.7%)and 33(38.7%)of the model and validation cohorts,respectively.Multivariate analysis identified age,tumor location,imaging of tumor boundary,blood type,mean hemoglobin concentration,neutrophil-tolymphocyte ratio,carbohydrate antigen 19-9,and carcinoembryonic antigen as independent risk factors for malignant PCN.The calibration curve indicated that the predictions based on the nomogram were in excellent agreement with the actual observations.A nomogram score cutoff of 192.5 classified patients as having low vs.high risk of malignant PCN.The model achieved good C-statistics of 0.929(95%CI 0.890–0.968,P<0.05)and 0.951(95%CI 0.903–0.998,P<0.05)in predicting malignancy in the development and validation cohorts,respectively.NRI=0.268;IDI=0.271(P<0.001 for improvement).The DCA curve indicated that our model yielded greater clinical benefits than the comparator model.Conclusions:The nomogram showed excellent performance in predicting malignant PCN and may help surgeons select patients for detailed examination and surgery.The nomogram is freely available at https://wangjunjinnomogram.shinyapps.io/DynNomapp/.
基金Supported by Major Scientific and Technological Special Project of Guangxi Zhuang Autonomous Region"Innovation and Demonstration of Ecological Comprehensive Prevention and Control Technology for Major Diseases of Livestock and Poultry"(GKAA17204057)Natural Science Foundation of Guangxi Zhuang Autonomous Region(2015GXNSFAA139070)+1 种基金Open-end Fund for Key Laboratory of Livestock Genetic Improvement of Guangxi Zhuang Autonomous Region(2014GXKLLGI-07)Project of Aquatic Animal Husbandry Science and Technology Extension and Application of Guangxi Zhuang Autonomous Region(GYMK201528030,GYMK201633034)
文摘[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs（ 22 males and 22 females） once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count（ RBC） and hemoglobin count（ HGB） both reached the lowest levels（ 6. 85 × 10^12/L and 97. 1 g/L）at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age（ P 〈 0. 05,similarly hereinafter）; mean corpuscular hemoglobin（ MCH） and mean corpuscular hemoglobin concentration（ MCHC） demonstrated basically the same variation pattern,which both reached the lowest levels（ 15. 11 pg and 275. 70 g/L） at 70 days of age and reached the highest levels（ 16. 40 pg and 314. 30 g/L） at 91 days of age; hematocrit（ HCT）,mean corpuscular volume（ MCV） and red blood cell distribution width（ RDW） exhibited relatively stable changes with no significant differences among different day ages（ P 〉 0. 05,similarly hereinafter）. Among white blood cell indexes of Guike pig at different day ages,white blood cell count（ WBC） reached the lowest level（ 13. 71 × 10^9/L） at 63 days of age and reached the highest level（ 17. 52 × 10^9/L） at 84 days of age,with significant differences; neutrophil count（ NEUT）reached the lowest level（ 3. 61 × 10^9/L） at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count（ LYMPH） exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count（ PLT）,thrombocytocrit（ PCT） and platelet volume distribution width（ PDW） exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume（ MPV）,PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age.
基金SPPH Incubator Fund for Development of Science and Technology(2021YJY-19)SPPH Foundation for Development of Science and Technology(2021BJ-26)International Science and Technology Cooperation Projects of Shaanxi Province(2022KW-14).
文摘Objective:To study the levels of serum cystatin C(Cys-C),total bile acid(TBA),and other routine blood parameters on patients with decompensated hepatitis B cirrhosis.Methods:Study group 1 consisted of 30 patients with hepatitis B-related decompensated cirrhosis,and study group 2 consisted of 30 patients with hepatitis B;while the control group consisted of 30 healthy people who underwent physical examination.The blood parameters were used to evaluate the clinical treatment effect of patients.Results:The TBA,Cys-C,alanine transaminase(ALT),total bilirubin(TBIL),aspartate aminotransferase(AST),and international normalized ratio(INR)in study group 1 were significantly higher than those of study group 2 and the control group;while the platelet count(PLT),hemoglobin(Hb),albumin(ALB),and estimated glomerular filtration rate(eGFR)were significantly lower in the study group 1 compared to the control group and study group 2(P<0.05).The Cys-C,PLT,TBA,AST,TBIL,and INR of patients in study group 1 who were successfully treated were significantly lower than the patients who were not successfully treated(P<0.05).Conclusion:Serum Cys-C,TBA,and routine blood parameters are useful in predicting the condition and the prognosis of patients of hepatitis B-related decompensated cirrhosis.
文摘Objective: to discuss the clinical value of new hemolysin and traditional hemolysin in blood routine examination. Methods: a total of 89 blood routine test subjects were selected from our hospital. The average control group (45 cases, using traditional hemolysin) and the study group (44 cases, using new hemolysin) were compared. Results: there were no statistically significant differences in the levels of red blood cells, white blood cells and platelets between the two groups (P > 0.05). The level of hemoglobin and satisfaction score in the study group were significantly higher than those in the control group (P < 0.05). The test time and cost in the study group were significantly lower than those in the control group (P < 0.05). Conclusion: the new hemolysin has more application value in routine blood test.
文摘Objective: this study focuses on the main causes and solutions of the routine blood test. Methods: in this study, 120 patients with the error in the routine blood test results from March 2020 to March 2021 were randomly selected as the study subjects, and the clinical data of all the study subjects were analyzed retrospectively. All patients received 2mL blood collection from peripheral blood and 14ml of venous blood. The venous blood samples were divided into 7 groups, among which group 1 was tested immediately after collection, groups 2 and 3 were stored in refrigerator (4℃) and room temperature, groups 4 and 4 hours respectively, group 6 and 7 were treated with 1.6 mg/ml and 2.8 mg/ml anticoagulant respectively. Peripheral blood needs to be tested immediately. The root causes of common errors are summarized, and the influence of different blood collection location, placement time and anticoagulant concentration on the routine blood test indicators is discussed, and the corresponding solutions are summarized. Results: the results of this study showed that platelet content in peripheral blood samples was significantly lower than venous blood, with significantly different data between groups (P <0.05). Blood samples were retained for 2 hours after collection, the hemoglobin concentration and platelet content were lower than the 4-hour retained samples, and the data differences between groups were significant (P <0.05).Blood samples were stored in the refrigerator, the hemoglobin concentration was lower than those stored at room temperature, and there were significant differences between groups (P <0.05).After the blood samples were treated with 2.8 mg/ml anticoagulant concentration, the content of each index was significantly higher than that treated with 1.6 mg/ml, and the data between the two groups varied significantly (P <0.05).Among the routine blood tests, 120 subjects had the error in the routine blood test indicators, including the unreasonable anticoagulant concentration, long inspection time, unreasonable storage and personal factors of the subjects. Conclusion: in the routine blood test, many factors resulted in the test error. The hospital should clarify the main influencing factors, improve the efficiency and quality of routine blood test by strengthening the training of relevant personnel, actively communicating with the subjects, and making reasonable use of anticoagulant, reducing the probability of error, and provide reliable support for the clinical diagnosis and treatment of doctors.
文摘The influence of exercise at high temperature on adult males＇ routine blood indexes and biochemical indexes and the expression of HSP72 in peripheral blood lymphocytes （PBLs） was studied in order to provide theoretical ground for health supervision of adults receiving exercise at high temperature. 180 adult males were selected and divided into exercise group and control group, in which the exercise group was subdivided into subgroup 1 and subgroup 2 receiving exercise at high temperature in the afternoon and in the morning, respectively. Peripheral venous blood was phle- botomized before and after the exercise to examine routine blood indexes and blood biochemical indexes. The expression levels of HSP72 in PBLs were detected by flow cytometry. The results showed that the routine blood indexes and biochemical indexes in each group were within the range of normal values of male adults. There was no significant difference between each exercise group and control group in indexes before exercise. After exercise, the expression levels of HSP72 in PBLs in exercise groups were higher than those before exercise, and HSP72 expression levels in subgroup 1 were obviously higher than those in subgroup 2 and control group. The contents of ALT, urea, Na＋, Cl-, Ca2＋ and K＋ in subgroups 1 and 2 were lower than those in control group, but CK level was higher than in control group （P〈0.05）. The contents of Na＋ and Clin subgroup 1 were relatively lower than those in subgroup 2 （P〈0.05）. It was concluded that while receiving exercise at high temperature, adult males＇ HSP72 levels in PBLs could be increased and the biochemical indexes changed. Attention should be paid to health supervision to avoid obvious body injuries at high temperature.
基金Key Program of Zhejiang Sports Bureau(2017(397)-09).
文摘Objective:To study the effect of training under high temperature on blood and heat shock protein 72(HSP72)expression of flying saucer athletes.Methods:The numbers of training group and control group are 30.Twenty-four flying saucer athletes in Zhejiang province were selected and randomly divided into training group and control group,15 in each group.Peripheral venous blood of each group was taken before and after the experiment.The HSP72 content of lymphocyte,blood routine and biochemical indexes were measured respectively.Results:There was significant difference in white blood cell in training group count before and after the experiment(P<0.05).The expression of HSP72 and the white blood cell in training group was higher than that control group(P<0.05).Conclusions:Organism could be affected and turned to stress state due to training under high temperature.It is necessary to provide the safeguard for the flying saucer athletes under high temperature in order to prevent the damage.
文摘To evaluate the subchronic and chronic toxicity of Fuyanxiao capsules,Sprague-Dawley(SD)rats were used in toxicity studies.In the subchronic toxicity study,50 female rats were randomly divided into a high-dose group(5.4g/kg/day)and a control group,with 15 rats in each,and medium(2.7g/kg/day)and low(1.35g/kg/day)dose groups,with 10 rats in each.The test substance was administered orally(mixed with feed,twice daily)for 90 consecutive days.In the chronic toxicity study,40 female rats were randomly divided into high,medium,and low dose groups and a control group,with 10 rats in each.The test substance was administered orally in the same manner for 180 consecutive days.Clinical signs,body weight,and food consumption were observed and recorded daily.At the end of the terminal phase(the first 10 rats from each group,1 day after the last dose)and the recovery phase(the last 5 rats from the control group and the high-dose group,observed for an additional 28 days after the last dose),blood and urine samples,as well as organs,were collected.Organ coefficients were calculated,and various hematological and urinary indicators were detected,followed by pathological analysis.The results showed that there were no significant differences in body weight,food consumption,or organ coefficients between any of the dose groups and the control group in both subchronic and chronic toxicity studies(P>0.05).Histopathological examination revealed no lesions,suggesting no tissue or organ damage in any of the dose groups.The rats exhibited good mental status,and hematological and urinary physiological indicators were within normal ranges,indicating stable liver and kidney function,hematopoietic system of the bone marrow,and internal environment in all dose groups.Therefore,Fuyanxiao capsule has no obvious subchronic or chronic toxicity in SD rats,and it is safe and reliable to use at reasonable dosage in clinical practice.
基金supported by the National Natural Science Foundation of China(Nos.81570416,81570337)Hubei Province Health and Family Planning Scientifie Research Project(No.WJ2019M120)+3 种基金Natural Science Foundation of Hubei Province(No.2019CFB668)the Fundamental Research Fund for the Central Universities(HUST)(No.2017KFYXJJ099)the Science and Technology Project Foundation of Wuhan(No.2017060201010175)the Outstanding Young Investigator Foundation of Tongji Hospital(No.YXQN009)。
文摘We here aimed to investigate the impact of gender on the clinical characteristics and laboratory results of patients with coronavirus disease 2019(COVID-19)and provide clues to the pathological mechanisms underlying COVID-19.A retrospective study was performed.Clinical characteristics,severity of lung infection,laboratory results,and prognoses of patients of different gender were analyzed.A total of 242 patients were finally included.The median age was 58 years(IQR:40-68),including 54(22.3%)hospital staffs.Ninety-four(38.8%)were male and 148(61.1%)were female.The proportion of patients with diabetes was significantly higher in the male group than in the female group(P=0.034).Male patients had a significantly larger proportion of severe lung infection,higher leukocyte count,neutrophil count,neutrophil-to-lymphocyte ratio,C-reactive protein,and procalcitonin than femalc.Furthermore,male patients had worse liver,cardiac,and coagulation function than their female counterparts.Male patients with COVID-19 showed more severe inflammation reaction and coagulation dysfunction than female patients.In conclusion,gender is associated with host response to SARS-CoV-2 infection.
基金National Natural Science Foundation of China(No.81660722)Natural Science Foundation of Tibet Autonomous Region(No.2015ZR-13-16)+1 种基金Scientific Research Project of Shaanxi Provincial Administration of Traditional Chinese Medicine(No.2019-ZZ-JC046)Key Project of Xizang Minzu University(No.13MYZP07)。
文摘Objective:To observe the effects of different hypoxia time on hematology and histomorphology of rats by simulating the environment of 7000m altitude,and to provide the basis for the establishment of acute hypoxia model.Methods:40 SD rats were randomly divided into 0h hypoxia group,6h,12h,24h,and 36h group.The hypoxia environment at 7000m was simulated by low-pressure oxygen chamber.The serum SOD,MDA,LDH biochemical indexes and the morphological changes of HE staining in heart,lung and brain tissues of rats were detected under different hypoxia time.Results:Compared with hypoxia group for 0h,the number of RBC in acute hypoxia group for 6h decreased and increased with the prolongation of hypoxia time,and the highest number of RBC.The WBC,LYMP,GRAN,HGB,HCT and RCT counts of the acute hypoxia group decreased for 6h,and the above indexes increased with the prolongation of hypoxia time.Compared with the 0h hypoxia group,SOD activity in serum decreased at different hypoxia time,and MDA content in the 24h and 36h hypoxia groups showed a fluctuating change and reached a peak at 12h hypoxia.The activity of LDH fluctuated,and reached the peak at 12hLDH after hypoxia,and then gradually decreased.He staining of the brain tissue of the heart and lung,which was close to normal after hypoxia for 36h,showed that with the prolongation of the hypoxia time,the damage degree of each organ increased continuously.Interstitial pneumonia or perivasculitis occurred in the lung,and perivascular edema and pulmonary interstitial widening were observed.Cardiac tissue showed obvious degeneration and necrosis of cardiomyocytes,lysis and necrosis of local myocardial interstitium and valve fibers.A large number of inflammatory cells could be seen infiltrating into the brain tissue in the CA2 CA3 DG region.The neuron cells were denatuated,the cell volume was reduced,basophilic was enhanced,and the nerve cells were triangular.The cells were pyknotic and basophilic,with a few transverse axis structures.Conclusion:acute low pressure hypoxia can cause red blood cells white blood cells within 6 h number of platelets Hemoglobin concentrations were lower,as after hypoxia prolonged period of count increased serum biochemical index SOD activity were lower,the content of MDA and activity of LDH outer-plane changes Can cause heart lung tissue pathological damage,and increased with prolonged.
