In this paper,algorithms for finding the inverse of a factor block circulant matrix, a factor block retrocirculant matrix and partitioned matrix with factor block circulant blocks over the complex field are presented ...In this paper,algorithms for finding the inverse of a factor block circulant matrix, a factor block retrocirculant matrix and partitioned matrix with factor block circulant blocks over the complex field are presented respectively.In addition,two algorithms for the inverse of a factor block circulant matrix over the quaternion division algebra are proposed.展开更多
To assess the merits of PEGylated poly （lactic-co-glycolic acid） （PEG-PLGA） nanoparticles as drug carriers for tumor necrosis factor-α receptor blocking peptide （TNFR-BP）, PEG-PLGA copolymer, which could be use...To assess the merits of PEGylated poly （lactic-co-glycolic acid） （PEG-PLGA） nanoparticles as drug carriers for tumor necrosis factor-α receptor blocking peptide （TNFR-BP）, PEG-PLGA copolymer, which could be used to prepare the stealth nanoparticles, was synthesized with methoxypolyethyleneglycol, DL-lactide and glycolide. The structure of PEG-PLGA was confirmed with ^1H-NMR and FT-IR spectroscopy, and the molecular weight （MW） was determined by gel permeation chromatography. Fluorescent FITC-TNFR- BP was chosen as model protein and encapsulated within PEG-PLGA nanoparticles using the double emulsion method. Atomic force microscopy and photon correlation spectroscopy were employed to characterize the stealth nanoparticles fabricated for morphology, size with polydispersity index and zeta potential. Encapsulation efficiency （EE） and the release of FITC-TNFR-BP in nanopartieles in vitro were measured by the fluorescence measurement. The stealth nanoparticles were found to have the mean diameter less than 270 nm and zeta potential less than -20 mV. In all nanoparticle formulations, more than 45% of EE were obtained. FITC-TNFR-BP release from the PEG-PLGA nanoparticles exhibited a biphasic pattern, initial burst release and consequently sustained release. The experimental results show that PEG-PLGA nanoparticles possess the potential to develop as drug carriers for controlled release applications of TNFR-BP.展开更多
A block representation of the BLU factorization for block tridiagonal matrices is presented. Some properties on the factors obtained in the course of the factorization are studied. Simpler expressions for errors incur...A block representation of the BLU factorization for block tridiagonal matrices is presented. Some properties on the factors obtained in the course of the factorization are studied. Simpler expressions for errors incurred at the process of the factorization for block tridiagonal matrices are considered.展开更多
Complicated geological structures make it difficult to analyze the stability of rock slopes, such as faults, weak intercalated layers or joint fissures. Based on 3D geological modeling and surface block identifying me...Complicated geological structures make it difficult to analyze the stability of rock slopes, such as faults, weak intercalated layers or joint fissures. Based on 3D geological modeling and surface block identifying methods, an integrated methodology framework was proposed and realized to analyze the stability of surface blocks in rock slopes. The surface blocks cut by geological structures, fissures or free faces could be identified subjected to the four principles of closure, completeness, uniqueness and validity. The factor of safety(FOS)of single key block was calculated by the limit equilibrium method. If there were two or more connected blocks, they were defined as a block-group. The FOS of a block-group was computed by the Sarma method. The proposed approach was applied to an actual rock slope of a hydropower project, and some possible instable blocks were demonstrated and analyzed visually. The obtained results on the key blocks or block-groups provide essential information for determining potential instable region of rock slopes and designing effective support scheme in advance.展开更多
Objective The aim of the study was to investigate the efficacy of immunocytochemistry and related gene detection using cell block for the diagnosis and individualized treatment of advanced lung cancer.Methods Sixty-fi...Objective The aim of the study was to investigate the efficacy of immunocytochemistry and related gene detection using cell block for the diagnosis and individualized treatment of advanced lung cancer.Methods Sixty-five malignant pleural effusion specimens were collected to make cell blocks, which were used for hematoxylin and eosin(H&E) staining, immunocytochemical studies, and gene sequencing of the tumors to guide the individualized diagnoses and treatment of the given tumors. Results The tumor cells in the cell block sections were abundant in number with high quality cellular structures, and the histological morphological characteristics were partially maintained. Immunocytochemical staining was helpful in identifying the cell origin and tumor classification, and amplification refractory mutation system(ARMS) was used to determine the mutation status of epidermal growth factor receptor(EGFR). Of the 65 samples, 50 had a diagnosis of adenocarcinoma, 7 were pulmonary squamous cells, 6 were small cell carcinoma of the lung, and 2 were mesothelioma. The morphological features of the tumors were as follows: acinar formation, papillary and single cells for adenocarcinoma;intercellular bridges for squamous cell carcinoma;and morphology of the small cells is similar to that of the smear. Correlating with the results of immunocytochemical staining and clinical data analysis, 40 cases were confirmed as pulmonary adenocarcinoma, with an additional 4 cases of breast cancer, 3 cases of ovarian adenocarcinoma, and 3 cases of colorectal adenocarcinoma. Of the 47 non-small cell lung carcinoma(NSCLC) patients, EGFR mutations were detected in 26 cases(55.3%) by ARMS, with four mutation types: exon 19 deletion(13 cases, 50.0%), exon 2l point mutations L858R(11 cases, 42.3%) and L861Q(1 case, 3.8%), and exon 18 point mutation G719X(1 case, 3.8%). Conclusion Malignant pleural effusion cell blocks combined with immunocytochemical markers and molecular pathology are helpful for the diagnosis of advanced tumors, the identification of tumor properties and histological tumor origin, and the selection of individualized treatment for advanced lung cancer.展开更多
目的研究孕妇血清叶酸(FA)、单核细胞趋化蛋白-1(MCP-1)和孕酮诱导的阻断因子(PIBF)水平与早期妊娠胚胎停止发育的相关性。方法选取2021年12月~2023年12月荆州市第二人民医院收治的早期妊娠胚胎停止发育孕妇98例为胎停组,另选同期进行...目的研究孕妇血清叶酸(FA)、单核细胞趋化蛋白-1(MCP-1)和孕酮诱导的阻断因子(PIBF)水平与早期妊娠胚胎停止发育的相关性。方法选取2021年12月~2023年12月荆州市第二人民医院收治的早期妊娠胚胎停止发育孕妇98例为胎停组,另选同期进行孕检的正常早期妊娠孕妇50例为对照组,收集一般临床资料,酶联免疫吸附试验(ELISA)检测血清FA,MCP-1和PIBF水平。多因素Logistic回归分析早期妊娠胚胎停止发育的影响因素;绘制受试者工作特征(ROC)曲线分析血清FA,MCP-1和PIBF对早期妊娠胚胎停止发育的预测价值;Pearson法分析血清FA,MCP-1,PIBF与孕酮(PROG)、雌二醇(E2)以及β-人绒毛膜促性腺激素(β-HCG)的相关性。结果与对照组相比,胎停组的血清FA(9.51±1.21nmol/L vs 11.32±1.56nmol/L),PIBF(295.46±30.22 ng/ml vs 342.14±36.97ng/ml)水平降低,血清MCP-1(1.02±0.15 mg/ml vs 0.82±0.11 mg/ml)水平升高,差异具有统计学意义(t=7.785,8.347,8.229,均P<0.001)。两组孕妇的胎停史(75.64%vs 25.36%),PROG(13.32±1.81 ng/ml vs 23.65±2.74 ng/ml),E2(221.34±25.69 pmol/L vs 298.65±31.64 pmol/L)以及β-HCG(5323.62±536.85 U/L vs 8562.31±924.55 U/L)水平比较,差异具有统计学意义(t/χ^(2)=6.548~27.428,均P<0.05)。孕妇胎停史、MCP-1水平升高为影响早期妊娠胎停的危险因素(Waldχ^(2)=4.239,4.613,均P<0.05),β-HCG,FA以及PIBF水平升高为影响早期妊娠胎停的保护因素(Waldχ^(2)=4.476,4.423,5.974,均P<0.05);FA,MCP-1,PIBF以及联合预测早期妊娠胚胎停止发育的AUC分别为0.811,0.805,0.816和0.908,联合预测显著优于FA,MCP-1,PIBF单独预测(Z=2.749,2.381,1.993,均P<0.05)。FA,PIBF与PROG,E2,β-HCG呈正相关(r=0.433~0.512,均P<0.05),MCP-1与PROG,E2,β-HCG呈负相关(r=-0.432,-0.487,-0.458,均P<0.05)。结论早期妊娠胚胎停止发育孕妇血清FA,PIBF水平降低,MCP-1水平升高,三者均为影响孕妇胚胎停止发育的影响因素,对早期妊娠胚胎停止发育具有一定辅助预测价值。展开更多
基金The research is partially supported by the 35th Postdoctoral Work of the National Science Foundation of China(2004035684).
文摘In this paper,algorithms for finding the inverse of a factor block circulant matrix, a factor block retrocirculant matrix and partitioned matrix with factor block circulant blocks over the complex field are presented respectively.In addition,two algorithms for the inverse of a factor block circulant matrix over the quaternion division algebra are proposed.
基金Funded by the National 863 Project of China (No. 2004AA215162)
文摘To assess the merits of PEGylated poly （lactic-co-glycolic acid） （PEG-PLGA） nanoparticles as drug carriers for tumor necrosis factor-α receptor blocking peptide （TNFR-BP）, PEG-PLGA copolymer, which could be used to prepare the stealth nanoparticles, was synthesized with methoxypolyethyleneglycol, DL-lactide and glycolide. The structure of PEG-PLGA was confirmed with ^1H-NMR and FT-IR spectroscopy, and the molecular weight （MW） was determined by gel permeation chromatography. Fluorescent FITC-TNFR- BP was chosen as model protein and encapsulated within PEG-PLGA nanoparticles using the double emulsion method. Atomic force microscopy and photon correlation spectroscopy were employed to characterize the stealth nanoparticles fabricated for morphology, size with polydispersity index and zeta potential. Encapsulation efficiency （EE） and the release of FITC-TNFR-BP in nanopartieles in vitro were measured by the fluorescence measurement. The stealth nanoparticles were found to have the mean diameter less than 270 nm and zeta potential less than -20 mV. In all nanoparticle formulations, more than 45% of EE were obtained. FITC-TNFR-BP release from the PEG-PLGA nanoparticles exhibited a biphasic pattern, initial burst release and consequently sustained release. The experimental results show that PEG-PLGA nanoparticles possess the potential to develop as drug carriers for controlled release applications of TNFR-BP.
文摘A block representation of the BLU factorization for block tridiagonal matrices is presented. Some properties on the factors obtained in the course of the factorization are studied. Simpler expressions for errors incurred at the process of the factorization for block tridiagonal matrices are considered.
基金Supported by the National Natural Science Foundation of China(No.51379006 and No.51321065)the Program for New Century Excellent Talents in University of Ministry of Education of China(NCET-12-0404)
文摘Complicated geological structures make it difficult to analyze the stability of rock slopes, such as faults, weak intercalated layers or joint fissures. Based on 3D geological modeling and surface block identifying methods, an integrated methodology framework was proposed and realized to analyze the stability of surface blocks in rock slopes. The surface blocks cut by geological structures, fissures or free faces could be identified subjected to the four principles of closure, completeness, uniqueness and validity. The factor of safety(FOS)of single key block was calculated by the limit equilibrium method. If there were two or more connected blocks, they were defined as a block-group. The FOS of a block-group was computed by the Sarma method. The proposed approach was applied to an actual rock slope of a hydropower project, and some possible instable blocks were demonstrated and analyzed visually. The obtained results on the key blocks or block-groups provide essential information for determining potential instable region of rock slopes and designing effective support scheme in advance.
基金Supported by a grant from the Natural Sciences Foundation of Inner Mongolia(No.2017MS08147)
文摘Objective The aim of the study was to investigate the efficacy of immunocytochemistry and related gene detection using cell block for the diagnosis and individualized treatment of advanced lung cancer.Methods Sixty-five malignant pleural effusion specimens were collected to make cell blocks, which were used for hematoxylin and eosin(H&E) staining, immunocytochemical studies, and gene sequencing of the tumors to guide the individualized diagnoses and treatment of the given tumors. Results The tumor cells in the cell block sections were abundant in number with high quality cellular structures, and the histological morphological characteristics were partially maintained. Immunocytochemical staining was helpful in identifying the cell origin and tumor classification, and amplification refractory mutation system(ARMS) was used to determine the mutation status of epidermal growth factor receptor(EGFR). Of the 65 samples, 50 had a diagnosis of adenocarcinoma, 7 were pulmonary squamous cells, 6 were small cell carcinoma of the lung, and 2 were mesothelioma. The morphological features of the tumors were as follows: acinar formation, papillary and single cells for adenocarcinoma;intercellular bridges for squamous cell carcinoma;and morphology of the small cells is similar to that of the smear. Correlating with the results of immunocytochemical staining and clinical data analysis, 40 cases were confirmed as pulmonary adenocarcinoma, with an additional 4 cases of breast cancer, 3 cases of ovarian adenocarcinoma, and 3 cases of colorectal adenocarcinoma. Of the 47 non-small cell lung carcinoma(NSCLC) patients, EGFR mutations were detected in 26 cases(55.3%) by ARMS, with four mutation types: exon 19 deletion(13 cases, 50.0%), exon 2l point mutations L858R(11 cases, 42.3%) and L861Q(1 case, 3.8%), and exon 18 point mutation G719X(1 case, 3.8%). Conclusion Malignant pleural effusion cell blocks combined with immunocytochemical markers and molecular pathology are helpful for the diagnosis of advanced tumors, the identification of tumor properties and histological tumor origin, and the selection of individualized treatment for advanced lung cancer.
文摘目的研究孕妇血清叶酸(FA)、单核细胞趋化蛋白-1(MCP-1)和孕酮诱导的阻断因子(PIBF)水平与早期妊娠胚胎停止发育的相关性。方法选取2021年12月~2023年12月荆州市第二人民医院收治的早期妊娠胚胎停止发育孕妇98例为胎停组,另选同期进行孕检的正常早期妊娠孕妇50例为对照组,收集一般临床资料,酶联免疫吸附试验(ELISA)检测血清FA,MCP-1和PIBF水平。多因素Logistic回归分析早期妊娠胚胎停止发育的影响因素;绘制受试者工作特征(ROC)曲线分析血清FA,MCP-1和PIBF对早期妊娠胚胎停止发育的预测价值;Pearson法分析血清FA,MCP-1,PIBF与孕酮(PROG)、雌二醇(E2)以及β-人绒毛膜促性腺激素(β-HCG)的相关性。结果与对照组相比,胎停组的血清FA(9.51±1.21nmol/L vs 11.32±1.56nmol/L),PIBF(295.46±30.22 ng/ml vs 342.14±36.97ng/ml)水平降低,血清MCP-1(1.02±0.15 mg/ml vs 0.82±0.11 mg/ml)水平升高,差异具有统计学意义(t=7.785,8.347,8.229,均P<0.001)。两组孕妇的胎停史(75.64%vs 25.36%),PROG(13.32±1.81 ng/ml vs 23.65±2.74 ng/ml),E2(221.34±25.69 pmol/L vs 298.65±31.64 pmol/L)以及β-HCG(5323.62±536.85 U/L vs 8562.31±924.55 U/L)水平比较,差异具有统计学意义(t/χ^(2)=6.548~27.428,均P<0.05)。孕妇胎停史、MCP-1水平升高为影响早期妊娠胎停的危险因素(Waldχ^(2)=4.239,4.613,均P<0.05),β-HCG,FA以及PIBF水平升高为影响早期妊娠胎停的保护因素(Waldχ^(2)=4.476,4.423,5.974,均P<0.05);FA,MCP-1,PIBF以及联合预测早期妊娠胚胎停止发育的AUC分别为0.811,0.805,0.816和0.908,联合预测显著优于FA,MCP-1,PIBF单独预测(Z=2.749,2.381,1.993,均P<0.05)。FA,PIBF与PROG,E2,β-HCG呈正相关(r=0.433~0.512,均P<0.05),MCP-1与PROG,E2,β-HCG呈负相关(r=-0.432,-0.487,-0.458,均P<0.05)。结论早期妊娠胚胎停止发育孕妇血清FA,PIBF水平降低,MCP-1水平升高,三者均为影响孕妇胚胎停止发育的影响因素,对早期妊娠胚胎停止发育具有一定辅助预测价值。
