Objective: To explore the proliferation inhibitory effect of quinones from Blaps rynchopetera defense secretion on colorectal tumor cell lines. Methods: Human colorectal cancer cell HT-29, human colorectal adenocarcin...Objective: To explore the proliferation inhibitory effect of quinones from Blaps rynchopetera defense secretion on colorectal tumor cell lines. Methods: Human colorectal cancer cell HT-29, human colorectal adenocarcinoma cell Caco-2 and normal human colon epithelial cell CCD841 were chosen for the evaluation of inhibitory activity of the main quinones of B. rynchopetera defense secretion, including methyl p-benzoquinone(MBQ), ethyl p-benzoquinone(EBQ), and methyl hydroquinone(MHQ), through methyl thiazolyl tetrazolium assay. The tumor-related factors, cell cycles, related gene expressions and protein levels were detected by enzyme-linked immunosorbent assy, flow cytometry, RT-polymerase chain reaction and Western blot, respectively. Results: MBQ, EBQ, and MHQ could significantly inhibit the proliferation of Caco-2, with half maximal inhibitory concentration(IC50) values of 7.04±0.88, 10.92±0.32, 9.35±0.83, HT-29, with IC50values of 14.90±2.71, 20.50±6.37, 13.90±1.30, and CCD841, with IC50values of 11.40±0.68, 7.02±0.44 and 7.83±0.05 μg/mL, respectively. Tested quinones can reduce the expression of tumor-related factors tumor necrosis factor α, interleukin(IL)-10, and IL-6 in HT-29 cells, selectively promote apoptosis, and regulate the cell cycle which can reduce the proportion of cells in the G1phase and increase the proportion of the S phase.Meanwhile, tested quinones could up-regulate mRNA and protein expression of GSK-3β and APC, while down-regulate that of β-catenin, Frizzled1, c-Myc, and CyclinD1 in the Wnt/β-catenin pathway of HT-29 cells.Conclusion: Quinones from B. rynchopetera defense secretion could inhibit the proliferation of colorectal tumor cells and reduce the expression of related factors, which would be functioned by regulating cell cycle, selectively promoting apoptosis, and affecting Wnt/β-catenin pathway-related mRNA and protein expressions.展开更多
With the increasing development of nanomaterials,the use of nanodiamonds(NDs)has been broadly manifested in many applications.However,their high penetration into the ecosystem indubitably poses remarkable toxicologica...With the increasing development of nanomaterials,the use of nanodiamonds(NDs)has been broadly manifested in many applications.However,their high penetration into the ecosystem indubitably poses remarkable toxicological risks.This paper investigates the toxic effects of NDs on the darkling beetle,Blaps polychresta Forskal,1775(Coleoptera:Tenebrionidae).Survival analysis was carried out by monitoring the beetles for 30 d after the injection of four different doses of NDs.A dose of 10.0 mg NDs/g body weight,causing less than 50%mortality effect,was assigned in the analysis of the different organs of studied beetles,including testis,ovary,and midgut.Structural and ultrastructural analyses were followed using light,TEM,and SEM microscopes.In addition,a variety of stress markers and enzyme activities were assessed using spectrophotometric methods.Furthermore,cell viability and DNA damage were evaluated using cytometry and comet assay,respectively.Compared to the control group,the NDs-treated group was exposed to various abnormalities within all the studied organs as follows.Significant disturbances in enzyme activities were accompanied by an apparent dysregulation in the antioxidant system.The flow cytometry results indicated a substantial decrease of viable cells along with a rise of apoptotic and necrotic cells.The comet assay demonstrated a highly increased level of DNA damage.Likewise,histological analyses accentuated the same findings showing remarkable deformities in the studied organs.Prominently,the research findings substantially contribute for the first time to evaluating the critical effects of NDs on B.polychresta,adopted as the bioindicator in this paper.展开更多
The escalating use of nanodiamonds(NDs)has raised concerns about their ecotoxicological impact,prompting exploration of therapeutic interventions.This paper pioneers the examination of Vitamin B12-conjugated sericin(V...The escalating use of nanodiamonds(NDs)has raised concerns about their ecotoxicological impact,prompting exploration of therapeutic interventions.This paper pioneers the examination of Vitamin B12-conjugated sericin(VB12-SER)as a potential therapeutic approach against ND-induced toxicity in darkling beetles(Blaps polychresta).The study analyzes mortality rates and organ-specific effects,covering the testis,ovary,and midgut,before and after treatments.Following exposure to 10 mg NDs/g body weight,within a subgroup of individuals termed ND2 with a mortality rate below 50%,two therapeutic treatments were administered,including pure sericin(SER)at 10 mg/mL and VB12-SER at 10.12 mg/mL.Consequently,five experimental groups(control,SER,ND2,ND2+SER,ND2+SER+VB12)were considered.Kaplan–Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30-d period.Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs,including cell death,DNA damage,enzyme dysregulation,antioxidant imbalances,protein depletion,lipid peroxidation,and morphological deformities.In contrast,the proposed treatments,especially(ND2+SER+VB12),demonstrated remarkable recovery,highlighting VB12-conjugated SER's potential in mitigating ND-triggered adverse effects.Molecular docking simulations affirmed binding stability and favorable interactions of the VB12-SER complex with target proteins.This research enhances understanding of NDs’effects on B.polychresta,proposing it as an effective bioindicator,and introduces VB12-conjugated SER as a promising therapeutic strategy in nanotoxicological studies.展开更多
基金National Natural Science Foundation of China(No.81960755)Bio-pharmaceutical Major Project of Yunnan Province(No.202002AA100007)+1 种基金Yunnan Fundamental Research Project(No.202001AU070022)Program for Innovative Research Team of Yunnan Province(No.202305AS350001)。
文摘Objective: To explore the proliferation inhibitory effect of quinones from Blaps rynchopetera defense secretion on colorectal tumor cell lines. Methods: Human colorectal cancer cell HT-29, human colorectal adenocarcinoma cell Caco-2 and normal human colon epithelial cell CCD841 were chosen for the evaluation of inhibitory activity of the main quinones of B. rynchopetera defense secretion, including methyl p-benzoquinone(MBQ), ethyl p-benzoquinone(EBQ), and methyl hydroquinone(MHQ), through methyl thiazolyl tetrazolium assay. The tumor-related factors, cell cycles, related gene expressions and protein levels were detected by enzyme-linked immunosorbent assy, flow cytometry, RT-polymerase chain reaction and Western blot, respectively. Results: MBQ, EBQ, and MHQ could significantly inhibit the proliferation of Caco-2, with half maximal inhibitory concentration(IC50) values of 7.04±0.88, 10.92±0.32, 9.35±0.83, HT-29, with IC50values of 14.90±2.71, 20.50±6.37, 13.90±1.30, and CCD841, with IC50values of 11.40±0.68, 7.02±0.44 and 7.83±0.05 μg/mL, respectively. Tested quinones can reduce the expression of tumor-related factors tumor necrosis factor α, interleukin(IL)-10, and IL-6 in HT-29 cells, selectively promote apoptosis, and regulate the cell cycle which can reduce the proportion of cells in the G1phase and increase the proportion of the S phase.Meanwhile, tested quinones could up-regulate mRNA and protein expression of GSK-3β and APC, while down-regulate that of β-catenin, Frizzled1, c-Myc, and CyclinD1 in the Wnt/β-catenin pathway of HT-29 cells.Conclusion: Quinones from B. rynchopetera defense secretion could inhibit the proliferation of colorectal tumor cells and reduce the expression of related factors, which would be functioned by regulating cell cycle, selectively promoting apoptosis, and affecting Wnt/β-catenin pathway-related mRNA and protein expressions.
文摘With the increasing development of nanomaterials,the use of nanodiamonds(NDs)has been broadly manifested in many applications.However,their high penetration into the ecosystem indubitably poses remarkable toxicological risks.This paper investigates the toxic effects of NDs on the darkling beetle,Blaps polychresta Forskal,1775(Coleoptera:Tenebrionidae).Survival analysis was carried out by monitoring the beetles for 30 d after the injection of four different doses of NDs.A dose of 10.0 mg NDs/g body weight,causing less than 50%mortality effect,was assigned in the analysis of the different organs of studied beetles,including testis,ovary,and midgut.Structural and ultrastructural analyses were followed using light,TEM,and SEM microscopes.In addition,a variety of stress markers and enzyme activities were assessed using spectrophotometric methods.Furthermore,cell viability and DNA damage were evaluated using cytometry and comet assay,respectively.Compared to the control group,the NDs-treated group was exposed to various abnormalities within all the studied organs as follows.Significant disturbances in enzyme activities were accompanied by an apparent dysregulation in the antioxidant system.The flow cytometry results indicated a substantial decrease of viable cells along with a rise of apoptotic and necrotic cells.The comet assay demonstrated a highly increased level of DNA damage.Likewise,histological analyses accentuated the same findings showing remarkable deformities in the studied organs.Prominently,the research findings substantially contribute for the first time to evaluating the critical effects of NDs on B.polychresta,adopted as the bioindicator in this paper.
文摘The escalating use of nanodiamonds(NDs)has raised concerns about their ecotoxicological impact,prompting exploration of therapeutic interventions.This paper pioneers the examination of Vitamin B12-conjugated sericin(VB12-SER)as a potential therapeutic approach against ND-induced toxicity in darkling beetles(Blaps polychresta).The study analyzes mortality rates and organ-specific effects,covering the testis,ovary,and midgut,before and after treatments.Following exposure to 10 mg NDs/g body weight,within a subgroup of individuals termed ND2 with a mortality rate below 50%,two therapeutic treatments were administered,including pure sericin(SER)at 10 mg/mL and VB12-SER at 10.12 mg/mL.Consequently,five experimental groups(control,SER,ND2,ND2+SER,ND2+SER+VB12)were considered.Kaplan–Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30-d period.Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs,including cell death,DNA damage,enzyme dysregulation,antioxidant imbalances,protein depletion,lipid peroxidation,and morphological deformities.In contrast,the proposed treatments,especially(ND2+SER+VB12),demonstrated remarkable recovery,highlighting VB12-conjugated SER's potential in mitigating ND-triggered adverse effects.Molecular docking simulations affirmed binding stability and favorable interactions of the VB12-SER complex with target proteins.This research enhances understanding of NDs’effects on B.polychresta,proposing it as an effective bioindicator,and introduces VB12-conjugated SER as a promising therapeutic strategy in nanotoxicological studies.
