Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly ...Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly understood.In this study,efforts were made to elucidate how MFT influences the fruit quality of Ponkan.The results showed that MFT substantially increased fruit size and elevated fruit total soluble solids in comparison with the fruit from the unthinned trees(used as control).Expression analyses demonstrated that m RNA abundance of three important sugar transporter genes,including CrSUT1,CrSTP1 and CrTMT1,was evidently elevated in the flesh of thinned fruit when compared with those of the control.In addition,MFT prominently up-regulated the transcript levels of various auxin and gibberellin(GA)biosynthesis and signaling genes,including CrYUC6,CrAUX/IAA,CrGA20ox1 and CrGA3ox1.Concurrently,the contents of endogenous IAA and GA3,measured at 90 d after fruit thinning,were notably elevated in the fruit from trees with the thinning treatment relative to the control,although no difference was detected in the two groups before the thinning manipulation.Taken together,these results indicate that manual fruit thinning could greatly improve fruit quality,which may be attributed to promoting fruit expansion due to the increased auxin levels and expediting sugar accumulation through the up-regulation of sugar transporter genes.展开更多
Objective:To assess the antioxidant activity of Ficus pseudopalma Blanco(Moraceae)(F.pseudopalma) and characterize the active components present in it.Methods:Column chromatography of crude ethanol leaf extract of F.p...Objective:To assess the antioxidant activity of Ficus pseudopalma Blanco(Moraceae)(F.pseudopalma) and characterize the active components present in it.Methods:Column chromatography of crude ethanol leaf extract of F.pseudopalma was performed and seven fractions were obtained,labeled as Ft.F2,F3,F4.F5,F6,F7.DPPH.FRAP,Criess,Kenton and superoxide radical scavenging assays were performed to assess the antioxidant ability of the fractions.Thin layer chromatography(TLC),high performance liquid chromatography and Fourier transfer infrared spectroscopy(FTIR) were performed to identify and characterize the bioactive component present in each fractions of F.pseudopalma.Results:DPPH and FRAP assay showed that F5.F6 and F7 exhibited the good proton accepting ability and reducing power as compared to the other fractions.All fractions exhibited a good nitric oxide radical scavenging activity wherein F1,F2 and F3 showed the highest inhibition.However,all of the fractions exhibited a stimulatory activity on hydroxyl and superoxide radicals.Lupeol matched one of the spots on the thin layer chromatography chromatogram of the fractions.Linear gradient high performance liquid chromatography and spiking of lupeol with the fraction revealed the presence of 5.84 mg/L lupeol in F6.Infrared spectra of the fractions revealed the presence of C-C,OH,aromatic C=C and C=O groups.Conclusions:The identified lupeol in F.pseudopalma may be responsible for the exhibited antioxidant property of the plant.Furthermore,knowing the antioxidant capability of the plant,F.pseudopalma can be developed into products which can help prevent the occurrence of oxidative stress related diseases.展开更多
Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica(Blanco)(Moraceae)(B. luzonica), an endemic plant in the Philippines.Methods:...Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica(Blanco)(Moraceae)(B. luzonica), an endemic plant in the Philippines.Methods: The air dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, namely, n-hexane,ethyl acetate and methanol to obtain three different extracts. Then, each of the extracts was further subjected to gas chromatography–mass spectrometry.Results: Qualitative determination of the different biologically active compounds from crude extracts of B. luzonica using gas chromatography–mass spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Furthermore, the three different extracts possess unique physicochemical characteristics which may be attributed to the compounds naturally present in significant quantities in the leaves of B. luzonica.Conclusions: The three extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of B. luzonica leaves warrants further biological and pharmacological studies.展开更多
Lunasia amara Blanco is a famous plant in South Sulawesi. It was used largely by local people as antibacteria and aphrodisiac. Quinoline alkaloid lunacridine was known as the active principle from Lunasia amara Blanco...Lunasia amara Blanco is a famous plant in South Sulawesi. It was used largely by local people as antibacteria and aphrodisiac. Quinoline alkaloid lunacridine was known as the active principle from Lunasia amara Blanco. Its activity was reported as a DNA Intercalating Topoisomerase II inhibitor. In this study, we have isolated and assayed the cytotoxic activity of lunacridine on P388 murine leukemia cells by MTT colorimetric assay （in vitro）. Lunacridine showed the less cytotoxic activity with the IC50 of 39.52 μg/mL. With the aim to explore the structural determinants responsible for this activity, molecular docking study have been carried out with DNA model using AutoDock 4.0 software with various total of energy evaluations （in silico）. The best docking reached at the total energy evaluations of 2.5 × 107 with the binding free energy of-6.63 kcal/mol. Analysis of the docking results was in accordance with the ability of lunacridine to intercalate between base pairs of DNA. Cytotoxic activity of lunacridine is less probably due to low affinity and molecular interaction. Therefore this study suggests to design and to develop lunacridine as a lead compound for anticancer drug.展开更多
基金supported by the Hubei Provincial Agriculture Research System,Chinathe Special Program for Technology Innovation of Hubei Province,China(2020BBA036)the Hubei Provincial Agricultural Science and Technology Innovation,China。
文摘Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly understood.In this study,efforts were made to elucidate how MFT influences the fruit quality of Ponkan.The results showed that MFT substantially increased fruit size and elevated fruit total soluble solids in comparison with the fruit from the unthinned trees(used as control).Expression analyses demonstrated that m RNA abundance of three important sugar transporter genes,including CrSUT1,CrSTP1 and CrTMT1,was evidently elevated in the flesh of thinned fruit when compared with those of the control.In addition,MFT prominently up-regulated the transcript levels of various auxin and gibberellin(GA)biosynthesis and signaling genes,including CrYUC6,CrAUX/IAA,CrGA20ox1 and CrGA3ox1.Concurrently,the contents of endogenous IAA and GA3,measured at 90 d after fruit thinning,were notably elevated in the fruit from trees with the thinning treatment relative to the control,although no difference was detected in the two groups before the thinning manipulation.Taken together,these results indicate that manual fruit thinning could greatly improve fruit quality,which may be attributed to promoting fruit expansion due to the increased auxin levels and expediting sugar accumulation through the up-regulation of sugar transporter genes.
基金Supported by Grants-in-aid of the Philippine Council for Health Research and Development,Department of Science and Technology(Grant no.120023)
文摘Objective:To assess the antioxidant activity of Ficus pseudopalma Blanco(Moraceae)(F.pseudopalma) and characterize the active components present in it.Methods:Column chromatography of crude ethanol leaf extract of F.pseudopalma was performed and seven fractions were obtained,labeled as Ft.F2,F3,F4.F5,F6,F7.DPPH.FRAP,Criess,Kenton and superoxide radical scavenging assays were performed to assess the antioxidant ability of the fractions.Thin layer chromatography(TLC),high performance liquid chromatography and Fourier transfer infrared spectroscopy(FTIR) were performed to identify and characterize the bioactive component present in each fractions of F.pseudopalma.Results:DPPH and FRAP assay showed that F5.F6 and F7 exhibited the good proton accepting ability and reducing power as compared to the other fractions.All fractions exhibited a good nitric oxide radical scavenging activity wherein F1,F2 and F3 showed the highest inhibition.However,all of the fractions exhibited a stimulatory activity on hydroxyl and superoxide radicals.Lupeol matched one of the spots on the thin layer chromatography chromatogram of the fractions.Linear gradient high performance liquid chromatography and spiking of lupeol with the fraction revealed the presence of 5.84 mg/L lupeol in F6.Infrared spectra of the fractions revealed the presence of C-C,OH,aromatic C=C and C=O groups.Conclusions:The identified lupeol in F.pseudopalma may be responsible for the exhibited antioxidant property of the plant.Furthermore,knowing the antioxidant capability of the plant,F.pseudopalma can be developed into products which can help prevent the occurrence of oxidative stress related diseases.
基金supported by Faculty of Pharmacy and Office of Grants, Endowments and Partnerships in Higher EducationUniversity of Santo Tomas, Manila, Philippines+2 种基金Nanocast Lab, Department of Chemistry, Mahidol University, Bangkok, ThailandDepartment of Science and Technology, PhilippinesCommission on Higher Education, Philippines
文摘Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica(Blanco)(Moraceae)(B. luzonica), an endemic plant in the Philippines.Methods: The air dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, namely, n-hexane,ethyl acetate and methanol to obtain three different extracts. Then, each of the extracts was further subjected to gas chromatography–mass spectrometry.Results: Qualitative determination of the different biologically active compounds from crude extracts of B. luzonica using gas chromatography–mass spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Furthermore, the three different extracts possess unique physicochemical characteristics which may be attributed to the compounds naturally present in significant quantities in the leaves of B. luzonica.Conclusions: The three extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of B. luzonica leaves warrants further biological and pharmacological studies.
文摘Lunasia amara Blanco is a famous plant in South Sulawesi. It was used largely by local people as antibacteria and aphrodisiac. Quinoline alkaloid lunacridine was known as the active principle from Lunasia amara Blanco. Its activity was reported as a DNA Intercalating Topoisomerase II inhibitor. In this study, we have isolated and assayed the cytotoxic activity of lunacridine on P388 murine leukemia cells by MTT colorimetric assay （in vitro）. Lunacridine showed the less cytotoxic activity with the IC50 of 39.52 μg/mL. With the aim to explore the structural determinants responsible for this activity, molecular docking study have been carried out with DNA model using AutoDock 4.0 software with various total of energy evaluations （in silico）. The best docking reached at the total energy evaluations of 2.5 × 107 with the binding free energy of-6.63 kcal/mol. Analysis of the docking results was in accordance with the ability of lunacridine to intercalate between base pairs of DNA. Cytotoxic activity of lunacridine is less probably due to low affinity and molecular interaction. Therefore this study suggests to design and to develop lunacridine as a lead compound for anticancer drug.
