Bistorta vivipara is a facultative reproductive plant capable of asexual reproduction through underground rhizomes and bulbils,as well as sexual reproduction via seeds.The phenomenon of vegetative organ vivipary is a ...Bistorta vivipara is a facultative reproductive plant capable of asexual reproduction through underground rhizomes and bulbils,as well as sexual reproduction via seeds.The phenomenon of vegetative organ vivipary is a complex biological process regulated by a network of genes.However,the developmental mechanism regulating bulbil vivipary in B.vivipara remains largely unexplored.This study investigated different developmental stages of B.vivipara using RNA sequencing and transcriptome analysis.Approximately 438 million high-quality reads were generated,with over 61.65%of the data mapped to the de novo transcriptome sequence.A total of 154,813 reads were matched in at least one public database,and 49,731 genes were differentially expressed across developmental stages.Functional analysis revealed significant enrichment of these genes in phenylpropanoid biosynthesis,plant hormone signal transduction,protein processing,starch and sucrose metabolism,and plant-pathogen interaction.Ninety-four genes involved in phytohormones,plant pigments,enzymes,and transcription factors were identified as potential candidates for inducing vegetative organ vivipary.These differentially expressed genes(DEGs),detected through comparative transcriptome analysis,may serve as candidate genes for bulbil vivipary in B.vivipara,establishing a foundation for future studies on the molecular mechanisms underlying vegetative organ vivipary.展开更多
Mongolian medicine and traditional Chinese medicine have different interpretations of Bistortae Rhizoma,including its name,nature and taste,function and efficacy.This paper sorted out the related application of Chines...Mongolian medicine and traditional Chinese medicine have different interpretations of Bistortae Rhizoma,including its name,nature and taste,function and efficacy.This paper sorted out the related application of Chinese and Mongolian medicine from the records of Materia Medica.The chemical components and pharmacological effects of Bistortae Rhizoma were summarized to provide a reference for the clinical application of Bistortae Rhizoma in traditional Chinese medicine and Mongolian medicine,and for the better development and utilization of national medicines.展开更多
SOC1(SUPPRESSOR OF OVEREXPRESSION OF CO1)编码的转录因子(TFs)隶属MADS-box基因家族,在植物开花调控和形态建成中发挥着重要作用。为揭示SOC1在珠芽蓼(Bistorta vivipara)花序珠芽形成过程中的作用,克隆了珠芽蓼BvSOC1基因(GenBank...SOC1(SUPPRESSOR OF OVEREXPRESSION OF CO1)编码的转录因子(TFs)隶属MADS-box基因家族,在植物开花调控和形态建成中发挥着重要作用。为揭示SOC1在珠芽蓼(Bistorta vivipara)花序珠芽形成过程中的作用,克隆了珠芽蓼BvSOC1基因(GenBank登记号为PP094486),并对不同海拔(2700、3300和3900 m)、不同发育时期(萌发期、膨大期、发芽期)珠芽中BvSOC1基因的表达进行分析。结果表明,BvSOC1基因开放阅读框为717 bp,编码蛋白包括238个氨基酸,含有MADS-box基因家族典型结构,与藜麦(Chenopodium quinoa)CqSOC1亲缘关系最近;BvSOC1基因的启动子含有响应水杨酸、脱落酸、光等逆境胁迫顺式作用元件;BvSOC1蛋白主要定位在细胞核;BvSOC1基因在珠芽蓼茎、叶、珠芽中均有表达,且在珠芽中的表达量最高;在同一海拔的珠芽蓼中,BvSOC1基因的表达量随着珠芽的发育显著上升;随着海拔的升高,BvSOC1基因的表达量在3个发育时期的珠芽中均显著上升;在紫外、低温胁迫下,BvSOC1基因在珠芽(2700 m,萌发期)中的表达量均呈现先上升后下降趋势。本研究为深入了解BvSOC1基因的功能、其在珠芽蓼珠芽发育中的作用及其与环境的关系提供参考。展开更多
基金supported by the National Natural Science Foundation of China(31960222)the Qinghai Provincial Major Science and Technology Special Funds(2023-NK-A3).
文摘Bistorta vivipara is a facultative reproductive plant capable of asexual reproduction through underground rhizomes and bulbils,as well as sexual reproduction via seeds.The phenomenon of vegetative organ vivipary is a complex biological process regulated by a network of genes.However,the developmental mechanism regulating bulbil vivipary in B.vivipara remains largely unexplored.This study investigated different developmental stages of B.vivipara using RNA sequencing and transcriptome analysis.Approximately 438 million high-quality reads were generated,with over 61.65%of the data mapped to the de novo transcriptome sequence.A total of 154,813 reads were matched in at least one public database,and 49,731 genes were differentially expressed across developmental stages.Functional analysis revealed significant enrichment of these genes in phenylpropanoid biosynthesis,plant hormone signal transduction,protein processing,starch and sucrose metabolism,and plant-pathogen interaction.Ninety-four genes involved in phytohormones,plant pigments,enzymes,and transcription factors were identified as potential candidates for inducing vegetative organ vivipary.These differentially expressed genes(DEGs),detected through comparative transcriptome analysis,may serve as candidate genes for bulbil vivipary in B.vivipara,establishing a foundation for future studies on the molecular mechanisms underlying vegetative organ vivipary.
基金Supported by Science and Technology Million Yuan Project of Inner Mongolia Medical University(YKD2018KJBW029,2019.07.1-2022.06.30)2021 Inner Mongolia Medical University Mongolian Medicine"First-class Discipline"Scientific Research Project(myxylxk202122)Collaborative Innovation Project of Inner Mongolia Autonomous Region(MYYXT202005,2020.09-2023.09).
文摘Mongolian medicine and traditional Chinese medicine have different interpretations of Bistortae Rhizoma,including its name,nature and taste,function and efficacy.This paper sorted out the related application of Chinese and Mongolian medicine from the records of Materia Medica.The chemical components and pharmacological effects of Bistortae Rhizoma were summarized to provide a reference for the clinical application of Bistortae Rhizoma in traditional Chinese medicine and Mongolian medicine,and for the better development and utilization of national medicines.
文摘SOC1(SUPPRESSOR OF OVEREXPRESSION OF CO1)编码的转录因子(TFs)隶属MADS-box基因家族,在植物开花调控和形态建成中发挥着重要作用。为揭示SOC1在珠芽蓼(Bistorta vivipara)花序珠芽形成过程中的作用,克隆了珠芽蓼BvSOC1基因(GenBank登记号为PP094486),并对不同海拔(2700、3300和3900 m)、不同发育时期(萌发期、膨大期、发芽期)珠芽中BvSOC1基因的表达进行分析。结果表明,BvSOC1基因开放阅读框为717 bp,编码蛋白包括238个氨基酸,含有MADS-box基因家族典型结构,与藜麦(Chenopodium quinoa)CqSOC1亲缘关系最近;BvSOC1基因的启动子含有响应水杨酸、脱落酸、光等逆境胁迫顺式作用元件;BvSOC1蛋白主要定位在细胞核;BvSOC1基因在珠芽蓼茎、叶、珠芽中均有表达,且在珠芽中的表达量最高;在同一海拔的珠芽蓼中,BvSOC1基因的表达量随着珠芽的发育显著上升;随着海拔的升高,BvSOC1基因的表达量在3个发育时期的珠芽中均显著上升;在紫外、低温胁迫下,BvSOC1基因在珠芽(2700 m,萌发期)中的表达量均呈现先上升后下降趋势。本研究为深入了解BvSOC1基因的功能、其在珠芽蓼珠芽发育中的作用及其与环境的关系提供参考。
