Sensors capable of recognizing cell surface carbohydrates,such as sialyl Lewis X（sLe-x）,are invaluable research tools and for the diagnosis and early detection of many forms of cancer.In this paper,we report the des...Sensors capable of recognizing cell surface carbohydrates,such as sialyl Lewis X（sLe-x）,are invaluable research tools and for the diagnosis and early detection of many forms of cancer.In this paper,we report the design and synthesis of a series of bisboronic acids 6（a-f） as fluorescent sensors towards mono-/oligosaccharides.Among them,compounds 6d and 6e showed strong binding affinities with glucose and fructose,while compound 6c,in which two anthracene-based boronic acid units were linked by a hexamethylene spacer,was able to recognize sLe-x selectivity and stained HEPG2 cells at 1 μmoI/L.展开更多
Lipid droplets(LDs)are dynamic intracellular organelles that participate in a wide range of physiological and pathological processes.Consequently,the development of high-selectivity and high-resolution tools for LD de...Lipid droplets(LDs)are dynamic intracellular organelles that participate in a wide range of physiological and pathological processes.Consequently,the development of high-selectivity and high-resolution tools for LD detection and tracking is of paramount importance.In this study,we describe the straightforward synthesis of a series of novel BODIPY analogs,BOQHYs 3a–3e,through the condensation of 2,3-dihydrazinylquinoline with acetone or benzophenone,followed by complexation with BF3⋅OEt2.Spectroscopic properties indicate that these dyes exhibited significantly larger Stokes shifts(>100 nm)than the commercial LD-Tracker BODIPY 493/503(≈10 nm).Additionally,the incorporation of phenyl“rotors”endows BOQHYs 3b–3e with heightened aggregation-induced emission activity,viscosity responsiveness,and exceptional lipophilicity,enabling their selective staining of LDs in a rapid and wash-free manner,with outstanding signal-to-noise ratios.Time-resolved confocal fluorescence imaging of 3d further validates these dyes’capability to effectively capture LD fusion and fission events,highlighting their potential applications in LD-related cell biology and disease diagnostics.展开更多
基金supported by the National Natural Science Foundation of China(NSFC)(No.21372060)Hebei Province Natural Science Fund for Distinguished Young Scholars(No.B2015201005)
文摘Sensors capable of recognizing cell surface carbohydrates,such as sialyl Lewis X（sLe-x）,are invaluable research tools and for the diagnosis and early detection of many forms of cancer.In this paper,we report the design and synthesis of a series of bisboronic acids 6（a-f） as fluorescent sensors towards mono-/oligosaccharides.Among them,compounds 6d and 6e showed strong binding affinities with glucose and fructose,while compound 6c,in which two anthracene-based boronic acid units were linked by a hexamethylene spacer,was able to recognize sLe-x selectivity and stained HEPG2 cells at 1 μmoI/L.
基金National Natural Science Foundation of China,Grant/Award Numbers:21871072,22401067The Interdisciplinary Research Project of Hangzhou Normal University,Grant/Award Number:2024JCXK01。
文摘Lipid droplets(LDs)are dynamic intracellular organelles that participate in a wide range of physiological and pathological processes.Consequently,the development of high-selectivity and high-resolution tools for LD detection and tracking is of paramount importance.In this study,we describe the straightforward synthesis of a series of novel BODIPY analogs,BOQHYs 3a–3e,through the condensation of 2,3-dihydrazinylquinoline with acetone or benzophenone,followed by complexation with BF3⋅OEt2.Spectroscopic properties indicate that these dyes exhibited significantly larger Stokes shifts(>100 nm)than the commercial LD-Tracker BODIPY 493/503(≈10 nm).Additionally,the incorporation of phenyl“rotors”endows BOQHYs 3b–3e with heightened aggregation-induced emission activity,viscosity responsiveness,and exceptional lipophilicity,enabling their selective staining of LDs in a rapid and wash-free manner,with outstanding signal-to-noise ratios.Time-resolved confocal fluorescence imaging of 3d further validates these dyes’capability to effectively capture LD fusion and fission events,highlighting their potential applications in LD-related cell biology and disease diagnostics.
