The influence of irradiation on biosyntheses of ascorbic acid and riboflavin in germinating chickpea seeds at ambient (25-35℃) conditions, was investigated. The rate of syntheses of these vitamins significantly incre...The influence of irradiation on biosyntheses of ascorbic acid and riboflavin in germinating chickpea seeds at ambient (25-35℃) conditions, was investigated. The rate of syntheses of these vitamins significantly increased with increasing germination time upto 120 h depending upon the treatment (P【0.05). Maximum amounts of ascorbic acid, 22.32 and 16.84 mg/100g, were found in the 0.10 kGy sample after 120 h of germination in tap and distilled water respectively. However, a radiation dose of 0.15 kGy resulted in the development of maximum values of riboflavin, 11.40 and 11.38 μ g/g, on germination in tap and distilled water respectively. A significant linear relation (r = 0-954 to 0.997) was observed between the biosyntheses of these vitamins and the germination time upto 120 h of irradiated and unirradiated chickpea seed (P【0.05).展开更多
In this study,Schwertmannite,Akaganéite and ammoniojarosite were biosynthesized by different bacteria and characterized.The results showed that bacteria are critical in mediating the mineral formation process:the...In this study,Schwertmannite,Akaganéite and ammoniojarosite were biosynthesized by different bacteria and characterized.The results showed that bacteria are critical in mediating the mineral formation process:the morphology,crystallinity,grain size and specific surface area of each mineral varied upon different bacteria and culturing conditions.In addition,the formed minerals’elemental composition and group disparity lead to different morphology,crystallinity and subsequent adsorption performance.In particular,adsorption difference existed in iron minerals biosynthesized by different bacteria.The maximal adsorption capacities of Akaganéite,Schwertmannite and ammoniojarosite were 26.6 mg/g,17.5 mg/g and 3.90 mg/g respectively.Cr(VI)adsorption on iron-minerals involves hydrogen bonding,electrostatic interaction,and ligand exchange.The adsorption only occurred on the surface of ammoniojarosite,while for Akaganéite and Schwertmannite,the tunnel structure greatly facilitated the adsorption process and improved adsorption capacity.Thus,the molecular structure is the primary determining factor for adsorption performance.Collectively,the results can provide useful information in selecting suitable bacteria for synthesizing heavy-metal scavenging minerals according to different environmental conditions.展开更多
The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of...The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles(Bio-AgNPs),gentamycin(GEN),and the conjugation of biosynthesized silver nanoparticles and gentamycin(GEN:Bio-AgNPs)on multidrugresistant Pseudomonas aeruginosa isolates.The characteristic properties of Bio-AgNPs were detected by the following analyses:ultraviolet–visible(UV–Vis)spectroscopy,Fourier transform infrared spectroscopy(FTIR),dynamic light scattering(DLS),Zeta potential,transmission electron microscopy(TEM),and scanning electron microscopy(SEM).Depending on micro-dilution assay,the minimum inhibitory concentration(MIC)of the tested subjects(Bio-AgNPs,GEN,GEN:Bio-AgNPs)are calculated to be 112±400,1536±525,and 49±18.8μg/mL,respectively.The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone,showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P.aeruginosa.展开更多
Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging metho...Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging method for accurate and rapid diagnosis of cancer cells by introducing aqueous Fe^2＋ and Zn^2＋ ions into cancer cells （i.e., HeLa, U87, and HepG2 cancer cells）. We found that the biocompatible metal ions Fe^2＋ and Zn^2＋ forced the cancer cells to spontaneously synthesize fluorescent ZnO nanoclusters and magnetic Fe3O4 nanoclusters. These clusters could then be used for multimodal cancer imaging by combining fluorescence imaging with magnetic resonance imaging and computed tomography imaging. Meanwhile, for normal cells （i.e., L02） and tissues, neither fluorescence nor any other obvious difference could be detected between pre- and post-injection. This multimodal bioimaging strategy based on the in situ biosynthesized Zn＆Fe oxide nanoclusters might therefore be useful for early cancer diagnosis and therapy.展开更多
文摘The influence of irradiation on biosyntheses of ascorbic acid and riboflavin in germinating chickpea seeds at ambient (25-35℃) conditions, was investigated. The rate of syntheses of these vitamins significantly increased with increasing germination time upto 120 h depending upon the treatment (P【0.05). Maximum amounts of ascorbic acid, 22.32 and 16.84 mg/100g, were found in the 0.10 kGy sample after 120 h of germination in tap and distilled water respectively. However, a radiation dose of 0.15 kGy resulted in the development of maximum values of riboflavin, 11.40 and 11.38 μ g/g, on germination in tap and distilled water respectively. A significant linear relation (r = 0-954 to 0.997) was observed between the biosyntheses of these vitamins and the germination time upto 120 h of irradiated and unirradiated chickpea seed (P【0.05).
基金Project(42277256)supported by the National Natural Science Foundation of ChinaProjects(HBKT-2021011,HBKT-2021014)supported by the Hunan Province Environmental Protection Research Program,ChinaProject(CDSKY-2023-05)supported by the Scientific Research of Project Hunan Provincial Urban Geological Survey and Monitoring Institute,China。
文摘In this study,Schwertmannite,Akaganéite and ammoniojarosite were biosynthesized by different bacteria and characterized.The results showed that bacteria are critical in mediating the mineral formation process:the morphology,crystallinity,grain size and specific surface area of each mineral varied upon different bacteria and culturing conditions.In addition,the formed minerals’elemental composition and group disparity lead to different morphology,crystallinity and subsequent adsorption performance.In particular,adsorption difference existed in iron minerals biosynthesized by different bacteria.The maximal adsorption capacities of Akaganéite,Schwertmannite and ammoniojarosite were 26.6 mg/g,17.5 mg/g and 3.90 mg/g respectively.Cr(VI)adsorption on iron-minerals involves hydrogen bonding,electrostatic interaction,and ligand exchange.The adsorption only occurred on the surface of ammoniojarosite,while for Akaganéite and Schwertmannite,the tunnel structure greatly facilitated the adsorption process and improved adsorption capacity.Thus,the molecular structure is the primary determining factor for adsorption performance.Collectively,the results can provide useful information in selecting suitable bacteria for synthesizing heavy-metal scavenging minerals according to different environmental conditions.
文摘The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles(Bio-AgNPs),gentamycin(GEN),and the conjugation of biosynthesized silver nanoparticles and gentamycin(GEN:Bio-AgNPs)on multidrugresistant Pseudomonas aeruginosa isolates.The characteristic properties of Bio-AgNPs were detected by the following analyses:ultraviolet–visible(UV–Vis)spectroscopy,Fourier transform infrared spectroscopy(FTIR),dynamic light scattering(DLS),Zeta potential,transmission electron microscopy(TEM),and scanning electron microscopy(SEM).Depending on micro-dilution assay,the minimum inhibitory concentration(MIC)of the tested subjects(Bio-AgNPs,GEN,GEN:Bio-AgNPs)are calculated to be 112±400,1536±525,and 49±18.8μg/mL,respectively.The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone,showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P.aeruginosa.
基金This work is supported by the National High-tech R＆D Program of China （No. 2015AA020502） and the National Natural Science Foundation of China （Nos. 81325011, 21327902 and 21175020）. M. S. acknowledges support from the NSF-PREM program （No, DRM-1523588）.
文摘Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging method for accurate and rapid diagnosis of cancer cells by introducing aqueous Fe^2＋ and Zn^2＋ ions into cancer cells （i.e., HeLa, U87, and HepG2 cancer cells）. We found that the biocompatible metal ions Fe^2＋ and Zn^2＋ forced the cancer cells to spontaneously synthesize fluorescent ZnO nanoclusters and magnetic Fe3O4 nanoclusters. These clusters could then be used for multimodal cancer imaging by combining fluorescence imaging with magnetic resonance imaging and computed tomography imaging. Meanwhile, for normal cells （i.e., L02） and tissues, neither fluorescence nor any other obvious difference could be detected between pre- and post-injection. This multimodal bioimaging strategy based on the in situ biosynthesized Zn＆Fe oxide nanoclusters might therefore be useful for early cancer diagnosis and therapy.
