Fibrosis is a pathological outcome of a dysregulated repair response to injury,which can occur in any organ and have devastating effects on hundreds of millions of patients worldwide.However,challenges remain in delin...Fibrosis is a pathological outcome of a dysregulated repair response to injury,which can occur in any organ and have devastating effects on hundreds of millions of patients worldwide.However,challenges remain in delineating the complex and dynamic network regulating fibrosis,as well as translating this information into effective anti-fibrotic treatments.A comprehensive understanding of existing methodologies and the development of new research tools are essential for ensuring the transferability of findings from bench to bedside.In this review,we present a framework consisting of a large biospecimen repository that integrates diverse patient cohorts with corresponding clinical data,and a systematic research platform incorporating multiple layers of experimental strategies,primarily focused on skin fibrosis.We summarize current advancements and the applications of various tools for preclinical fibrosis research and examine the limitations of traditional methods used to simulate and investigate biomechanical signals in the fibrotic environment.Importantly,we highlight the strengths of research techniques and translational approaches of varying physiological relevance developed by us over the past decade.Collectively,we emphasize a trend toward more faithfully replicating the functional,structural,and biological complexity of fibrosis while providing high spatio-temporal control over soluble cues and intricate interactions.Our comprehensive overview of methodology paves the way for minimizing batch-to-batch variation and improving the reproducibility of experimental systems.展开更多
UK Biobank是一项庞大和详细的前瞻性研究工程,由维康信托基金、英国医学研究委员会、英国卫生部、苏格兰政府和西北地区发展局发起成立。其储存着海量疾病与健康相关的研究数据和生物样本,全球的科研人员均可通过在线申请获得这些研究...UK Biobank是一项庞大和详细的前瞻性研究工程,由维康信托基金、英国医学研究委员会、英国卫生部、苏格兰政府和西北地区发展局发起成立。其储存着海量疾病与健康相关的研究数据和生物样本,全球的科研人员均可通过在线申请获得这些研究数据和生物样本并将其用于已批准的研究项目中。本文详细介绍了如何通过UK Biobank检索及申请研究数据和生物样本。展开更多
基金supported by grants from the National Natural Science Foundation of China(grant nos.82472554 and 82202449)the Fund for Excellent Young Scholars of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(grant no.JYYQ006)the Shanghai Clinical Research Center of Plastic and Reconstructive Surgery(grant no.22MC1940300).
文摘Fibrosis is a pathological outcome of a dysregulated repair response to injury,which can occur in any organ and have devastating effects on hundreds of millions of patients worldwide.However,challenges remain in delineating the complex and dynamic network regulating fibrosis,as well as translating this information into effective anti-fibrotic treatments.A comprehensive understanding of existing methodologies and the development of new research tools are essential for ensuring the transferability of findings from bench to bedside.In this review,we present a framework consisting of a large biospecimen repository that integrates diverse patient cohorts with corresponding clinical data,and a systematic research platform incorporating multiple layers of experimental strategies,primarily focused on skin fibrosis.We summarize current advancements and the applications of various tools for preclinical fibrosis research and examine the limitations of traditional methods used to simulate and investigate biomechanical signals in the fibrotic environment.Importantly,we highlight the strengths of research techniques and translational approaches of varying physiological relevance developed by us over the past decade.Collectively,we emphasize a trend toward more faithfully replicating the functional,structural,and biological complexity of fibrosis while providing high spatio-temporal control over soluble cues and intricate interactions.Our comprehensive overview of methodology paves the way for minimizing batch-to-batch variation and improving the reproducibility of experimental systems.
