Cardiovascular diseases pose a significant global health challenge,driving ongoing efforts to develop effective treatments.Various biofabrication technologies utilizing numerous materials have been employed to design ...Cardiovascular diseases pose a significant global health challenge,driving ongoing efforts to develop effective treatments.Various biofabrication technologies utilizing numerous materials have been employed to design functional cardiac tissues.Choosing the right material is crucial to support cardiac cell growth,proliferation,tissue maturation and functionality.3D printing enables the fabrication of structures that mimic the hierarchical organization of native cardiac tissue,further enhancing its function.Electrospinning produces nanofibrous scaffolds with a high surface area and porosity,mimicking the extracellular matrix and promoting the cell be-haviors required for tissue formation.Although typically employed independently,combining these technologies can enable the fabrication of patches with properties closely resembling those of native cardiac tissues.Recent research focuses on the use of electroconductive materials,which enhance cell-to-cell communication and promote the maturation of cardiomyocytes,thereby preventing arrhythmic contractions and improving the functionality of engineered cardiac tissues.In this review,recent studies showcasing the applications of elec-troconductive biopolymer-based fibrous materials and hydrogels designed using 3D printing and/or electro-spinning for cardiac tissue engineering are discussed.Furthermore,the review evaluates the synergistic effects of biopolymer-based materials and electrical components in 3D printed electroconductive hydrogels.It also dis-cusses the challenges faced in fabricating these hydrogels and explores their future prospects for biomedical applications.展开更多
Natural biopolymer-based hydrogels have been extensively studied in recent years due to their excellent biocompatibility.However,the preparation of multi-functional and tough natural biopolymer-based hydrogels is stil...Natural biopolymer-based hydrogels have been extensively studied in recent years due to their excellent biocompatibility.However,the preparation of multi-functional and tough natural biopolymer-based hydrogels is still a challenging problem.Herein,a natural biopolymer-based hydrogel is prepared using gelatin and carboxymethyl chitosan(CMCS)through a one-step soaking method.The prepared hydrogel without any synthetic polymers and crosslinking agents has a fully physical crosslinking structure.Due to the hydrophobic interaction brought by the Hofmeister effect,the mechanical properties of soaked hydrogels(tensile stress and strain can reach 3.77 MPa and 1082%)are superior to conventional protein hydrogels.In addition,the prepared gelatin/carboxymethyl chitosan(Gel/CMCS)hydrogels exhibit a variety of appealing properties,including good shape memory,fatigue resistance,electrical conductivity,water retention,drug releasing,antibacterial property,and recyclability.This strategy opens up a new horizon to fabricate hydrogels with excellent mechanical properties and multiple functions,which can extend their applications in the biomedicine area and other related fields.展开更多
基金support from the Emmy Noether Programme of the German Research Foundation DFG(Grant number:OS 497/6-1,AOBJ:660891)the Walter Benjamin Programme of the German Research Foundation DFG(Grant number:KA 6604/2-1,AOBJ:708768)the German Heart Foundation e.V.(to F.B.E.).
文摘Cardiovascular diseases pose a significant global health challenge,driving ongoing efforts to develop effective treatments.Various biofabrication technologies utilizing numerous materials have been employed to design functional cardiac tissues.Choosing the right material is crucial to support cardiac cell growth,proliferation,tissue maturation and functionality.3D printing enables the fabrication of structures that mimic the hierarchical organization of native cardiac tissue,further enhancing its function.Electrospinning produces nanofibrous scaffolds with a high surface area and porosity,mimicking the extracellular matrix and promoting the cell be-haviors required for tissue formation.Although typically employed independently,combining these technologies can enable the fabrication of patches with properties closely resembling those of native cardiac tissues.Recent research focuses on the use of electroconductive materials,which enhance cell-to-cell communication and promote the maturation of cardiomyocytes,thereby preventing arrhythmic contractions and improving the functionality of engineered cardiac tissues.In this review,recent studies showcasing the applications of elec-troconductive biopolymer-based fibrous materials and hydrogels designed using 3D printing and/or electro-spinning for cardiac tissue engineering are discussed.Furthermore,the review evaluates the synergistic effects of biopolymer-based materials and electrical components in 3D printed electroconductive hydrogels.It also dis-cusses the challenges faced in fabricating these hydrogels and explores their future prospects for biomedical applications.
基金This research was supported by Project of the Jilin Provincial Science and Technology Department,China(No.20190201078JC).
文摘Natural biopolymer-based hydrogels have been extensively studied in recent years due to their excellent biocompatibility.However,the preparation of multi-functional and tough natural biopolymer-based hydrogels is still a challenging problem.Herein,a natural biopolymer-based hydrogel is prepared using gelatin and carboxymethyl chitosan(CMCS)through a one-step soaking method.The prepared hydrogel without any synthetic polymers and crosslinking agents has a fully physical crosslinking structure.Due to the hydrophobic interaction brought by the Hofmeister effect,the mechanical properties of soaked hydrogels(tensile stress and strain can reach 3.77 MPa and 1082%)are superior to conventional protein hydrogels.In addition,the prepared gelatin/carboxymethyl chitosan(Gel/CMCS)hydrogels exhibit a variety of appealing properties,including good shape memory,fatigue resistance,electrical conductivity,water retention,drug releasing,antibacterial property,and recyclability.This strategy opens up a new horizon to fabricate hydrogels with excellent mechanical properties and multiple functions,which can extend their applications in the biomedicine area and other related fields.
