Supramolecular proteins are generated using a limited set of twenty amino acids,but have distinctive functionalities which arise from the sequential arrangement of amino acids configured to exquisite three-dimensional...Supramolecular proteins are generated using a limited set of twenty amino acids,but have distinctive functionalities which arise from the sequential arrangement of amino acids configured to exquisite three-dimensional structures.Viruses,virus-like particles,ferritins,enzyme complexes,cellular micro-compartments,and other supramolecular protein assemblies exemplify these systems,with their precise arrangements of tens to hundreds of molecules into highly organized scaffolds for nucleic acid packaging,metal storage,catalysis or sequestering reactions at the nanometer scale.These versatile protein systems,dubbed as bionanoparticles(BNPs),have attracted materials scientists to seek new opportunities with these pre-fabricated templates in a wide range of nanotechnology-related applications.Here,we focus on some of the key modification strategies that have been utilized,ranging from basic protein conjugation techniques to more novel strategies,to expand the functionalities of these multimeric protein assemblies.Ultimately,in combination with molecular cloning and sophisticated chemistries,these BNPs are being incorporated into many applications ranging from functional materials to novel biomedical drug designs.展开更多
Bionanoparticles(BNPs),consisting of virus and virus-like assemblies,have attracted much attention in the biomedical field for their applications such as imaging and targeted drug delivery,owing to their well-defined ...Bionanoparticles(BNPs),consisting of virus and virus-like assemblies,have attracted much attention in the biomedical field for their applications such as imaging and targeted drug delivery,owing to their well-defined structures and well-controlled chemistries.BNPs-based core-shell structures provide a unique system for the investigation of biological interactions such as protein-protein and protein-carbohydrate interactions.However,it is still a challenge to prepare the BNPs-based core-shell structures.Herein,we describe(i) co-assembly method and(ii) template synthesis method in the development of polymer-BNPs core-shell structures.These two methods can be divided into three different systems.In system A,different polymers including poly(2-vinylpyridine)(P2VP),poly(4-vinylpyridine)(P4VP) and poly(ε-caprolactone)-block-poly(2-vinylpyridine)(PCL-b-P2VP) can form a raspberry-like structure with BNPs.In system B,polystyrene(PS) spheres end capped with free amine and BNPs can form a core-shell structure.In System C,layer-by-layer(LBL) method is used to prepare positive charged PS particles,which can be used as a template to form the core-shell structures with BNPs.These two methods may open a new way for preparing novel protein-based functional materials for potential applications in the biomedical field.展开更多
A sol-gel process has been developed to incorporate bionanoparticles,such as turnip yellow mosaic virus,cowpea mosaic virus,tobacco mosaic virus,and ferritin into silica,while maintaining the integrity and morphology ...A sol-gel process has been developed to incorporate bionanoparticles,such as turnip yellow mosaic virus,cowpea mosaic virus,tobacco mosaic virus,and ferritin into silica,while maintaining the integrity and morphology of the particles.The structures of the resulting materials were characterized by transmission electron microscopy,small angle X-ray scattering,and N2 adsorption desorption analysis.The results show that the shape and surface morphology of the bionanoparticles are largely preserved after being embedded into silica.After removal of the bionanoparticles by calcination,mesoporous silica with monodisperse pores,having the shape and surface morphology of the bionanoparticles replicated inside the silica,was produced,.This study is expected to lead to both functional composite materials and mesoporous silica with structurally well-defi ned large pores.展开更多
Nano-biotechnology is recognized as offering revolutionary changes in various fields of medicine.Biologically synthesized silver nanoparticles have a wide range of applications.The biosynthesis of silver nanoparticles...Nano-biotechnology is recognized as offering revolutionary changes in various fields of medicine.Biologically synthesized silver nanoparticles have a wide range of applications.The biosynthesis of silver nanoparticles is an eco-friendly method in the field of nanotechnology.Seaweed extracts of Caulerpa racemosa(Forsskål)J.Agardh and Ulva lactuca Linnaeus was used as a reducing agent in the eco-friendly extracellular synthesis of silver nanoparticles from an aqueous solution of silver nitrate(AgNO3).High conversion of silver ions to silver nanoparticles was achieved with Ulva lactuca at reaction temperature of 100°C and a seaweed extract concentration of 10%with a residential time of 1 h using reflux extractor when compared with the other methods.Formation of silver nanoparticles was characterized by spectrophotometry and the electron microscopic technique.The average particles size was ranging from 35 to 75 nm.Antimicrobial activities indicate the minimum inhibitory concentration of biologically synthesized nanoparticles tested against the pathogen Staphylococcus aureus(1 mg/ml).High inhibitions over the growth of Pseudomonas aeruginosa,Vibrio cholerae and Escherichia coli were witnessed against the concentrations of 1 mg/ml.Enzyme assay of the collected seaweeds performed using standard protocol to assess the potency level.Further,seed germination test proved that synthesized nanoparticles were environmentally safe,for which the same can be used for effluent treatment process.展开更多
文摘Supramolecular proteins are generated using a limited set of twenty amino acids,but have distinctive functionalities which arise from the sequential arrangement of amino acids configured to exquisite three-dimensional structures.Viruses,virus-like particles,ferritins,enzyme complexes,cellular micro-compartments,and other supramolecular protein assemblies exemplify these systems,with their precise arrangements of tens to hundreds of molecules into highly organized scaffolds for nucleic acid packaging,metal storage,catalysis or sequestering reactions at the nanometer scale.These versatile protein systems,dubbed as bionanoparticles(BNPs),have attracted materials scientists to seek new opportunities with these pre-fabricated templates in a wide range of nanotechnology-related applications.Here,we focus on some of the key modification strategies that have been utilized,ranging from basic protein conjugation techniques to more novel strategies,to expand the functionalities of these multimeric protein assemblies.Ultimately,in combination with molecular cloning and sophisticated chemistries,these BNPs are being incorporated into many applications ranging from functional materials to novel biomedical drug designs.
基金support from the US NSF CAREER program,US DoD (W911NF-09-1-0236),the Alfred P. Sloan Scholarship, the Camille Dreyfus Teacher Scholar Award, DoD-BCRP,and the W.M.Keck Foundation
文摘Bionanoparticles(BNPs),consisting of virus and virus-like assemblies,have attracted much attention in the biomedical field for their applications such as imaging and targeted drug delivery,owing to their well-defined structures and well-controlled chemistries.BNPs-based core-shell structures provide a unique system for the investigation of biological interactions such as protein-protein and protein-carbohydrate interactions.However,it is still a challenge to prepare the BNPs-based core-shell structures.Herein,we describe(i) co-assembly method and(ii) template synthesis method in the development of polymer-BNPs core-shell structures.These two methods can be divided into three different systems.In system A,different polymers including poly(2-vinylpyridine)(P2VP),poly(4-vinylpyridine)(P4VP) and poly(ε-caprolactone)-block-poly(2-vinylpyridine)(PCL-b-P2VP) can form a raspberry-like structure with BNPs.In system B,polystyrene(PS) spheres end capped with free amine and BNPs can form a core-shell structure.In System C,layer-by-layer(LBL) method is used to prepare positive charged PS particles,which can be used as a template to form the core-shell structures with BNPs.These two methods may open a new way for preparing novel protein-based functional materials for potential applications in the biomedical field.
基金from NSF-DMR-0706431,NSF Career Award,the Camille Dreyfus Teacher-Scholarshipthe Alfred P.Sloan Foundation,US ARO MURI program,and the W.M.Keck Foundation+1 种基金The SAXS data were obtained at beamline X21 of the National Synchrotron Light Source,Brookhaven National LaboratoryThe use of NSLS was supported by the U.S.Department of Energy,Office of Science,Office of Basic Energy Sciences,under Contract No.DE-AC02-98CH10886.
文摘A sol-gel process has been developed to incorporate bionanoparticles,such as turnip yellow mosaic virus,cowpea mosaic virus,tobacco mosaic virus,and ferritin into silica,while maintaining the integrity and morphology of the particles.The structures of the resulting materials were characterized by transmission electron microscopy,small angle X-ray scattering,and N2 adsorption desorption analysis.The results show that the shape and surface morphology of the bionanoparticles are largely preserved after being embedded into silica.After removal of the bionanoparticles by calcination,mesoporous silica with monodisperse pores,having the shape and surface morphology of the bionanoparticles replicated inside the silica,was produced,.This study is expected to lead to both functional composite materials and mesoporous silica with structurally well-defi ned large pores.
文摘Nano-biotechnology is recognized as offering revolutionary changes in various fields of medicine.Biologically synthesized silver nanoparticles have a wide range of applications.The biosynthesis of silver nanoparticles is an eco-friendly method in the field of nanotechnology.Seaweed extracts of Caulerpa racemosa(Forsskål)J.Agardh and Ulva lactuca Linnaeus was used as a reducing agent in the eco-friendly extracellular synthesis of silver nanoparticles from an aqueous solution of silver nitrate(AgNO3).High conversion of silver ions to silver nanoparticles was achieved with Ulva lactuca at reaction temperature of 100°C and a seaweed extract concentration of 10%with a residential time of 1 h using reflux extractor when compared with the other methods.Formation of silver nanoparticles was characterized by spectrophotometry and the electron microscopic technique.The average particles size was ranging from 35 to 75 nm.Antimicrobial activities indicate the minimum inhibitory concentration of biologically synthesized nanoparticles tested against the pathogen Staphylococcus aureus(1 mg/ml).High inhibitions over the growth of Pseudomonas aeruginosa,Vibrio cholerae and Escherichia coli were witnessed against the concentrations of 1 mg/ml.Enzyme assay of the collected seaweeds performed using standard protocol to assess the potency level.Further,seed germination test proved that synthesized nanoparticles were environmentally safe,for which the same can be used for effluent treatment process.
