Objective:To elucidate the biological basis of the heart qi deficiency(HQD)pattern,an in-depth understanding of which is essential for improving clinical herbal therapy.Methods: We predicted and characterized HQD patt...Objective:To elucidate the biological basis of the heart qi deficiency(HQD)pattern,an in-depth understanding of which is essential for improving clinical herbal therapy.Methods: We predicted and characterized HQD pattern genes using the new strategy,TCM-HIN2Vec,which involves heterogeneous network embedding and transcriptomic experiments.First,a heterogeneous network of traditional Chinese medicine(TCM)patterns was constructed using public databases.Next,we predicted HQD pattern genes using a heterogeneous network-embedding algorithm.We then analyzed the functional characteristics of HQD pattern genes using gene enrichment analysis and examined gene expression levels using RNA-seq.Finally,we identified TCM herbs that demonstrated enriched interactions with HQD pattern genes via herbal enrichment analysis.Results: Our TCM-HIN2Vec strategy revealed that candidate genes associated with HQD pattern were significantly enriched in energy metabolism,signal transduction pathways,and immune processes.Moreover,we found that these candidate genes were significantly differentially expressed in the transcriptional profile of mice model with heart failure with a qi deficiency pattern.Furthermore,herbal enrichment analysis identified TCM herbs that demonstrated enriched interactions with the top 10 candidate genes and could potentially serve as drug candidates for treating HQD.Conclusion: Our results suggested that TCM-HIN2Vec is capable of not only accurately identifying HQD pattern genes,but also deciphering the basis of HQD pattern.Furthermore our finding indicated that TCM-HIN2Vec may be further expanded to develop other patterns,leading to a new approach aimed at elucidating general TCM patterns and developing precision medicine.展开更多
Objective:To investigate the biological basis of“depression with liver-qi stagnation and spleen deficiency syndrome”.Methods:A digital gene expression profiling method was conducted to explore global changes in the ...Objective:To investigate the biological basis of“depression with liver-qi stagnation and spleen deficiency syndrome”.Methods:A digital gene expression profiling method was conducted to explore global changes in the mRNA transcriptome in a rat model of depression with liver-qi stagnation and spleen deficiency syndrome.Real-time quantitative polymerase chain reaction(q-PCR)was performed to verify the five genes most interest based on the Kyoto Encyclopedia of Genes and Genome(KEGG)analysis.Sini San,which disperses stagnated liver qi and strengthens the spleen,was administered to the model rats to observe whether it could reverse these genetic changes in the liver.Results:Forty-six differentially expressed genes were identified.Three of the five genes of most interestdHnf4a,Hnf4g and Cyp1a1dbased on KEGG analysis,were confirmed by realtime q-PCR.Sini San reduced the gene expression changes of Hnf4a,Hnf4g and Cyp1a1 in the rat model.Conclusions:Hnf4a,Hnf4g and Cyp1a1 are involved in“depression with liver-qi stagnation and spleen deficiency syndrome”.These findings indicate that depressed rats with liver-qi stagnation and spleen deficiency syndrome are at risk of liver diseases.Furthermore,our results will inform exploration of the etiology of depression and help in the development of effective therapeutic strategies.展开更多
Researchers have discovered that mice instinctively exhibit rescue-like behavior towards anesthetized companions-a finding that suggests a biological basis for prosociality.The study,published in PNAS on April 23,2025...Researchers have discovered that mice instinctively exhibit rescue-like behavior towards anesthetized companions-a finding that suggests a biological basis for prosociality.The study,published in PNAS on April 23,2025,was led by Dr.HU Li from the Institute of Psychology of the Chinese Academy of Sciences(IPCAS)and Dr.CHEN Zhoufeng from Washington University School of Medicine and the Shenzhen Medical Academy of Research and Translation.展开更多
BACKGROUND Gastrointestinal cancers are among the most commonly diagnosed cancers globally.Traditional Chinese medicine(TCM)offers distinct advantages in preventing and treating these cancers.AIM To investigate the me...BACKGROUND Gastrointestinal cancers are among the most commonly diagnosed cancers globally.Traditional Chinese medicine(TCM)offers distinct advantages in preventing and treating these cancers.AIM To investigate the metabolic basis of a common TCM syndrome in gastrointestinal cancers,exploring underlying metabolic mechanisms and identifying potential METHODS Thirty healthy controls(normal group),30 patients with gastric cancer(GC),and 30 patients with colorectal cancer(CRC)were enrolled in 2023.Plasma metabolic profiles were detected using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry,and pathway enrichment analysis was conducted using the Kyoto Encyclopedia of Genes and Genomes.RESULTS Metabolic profiling revealed distinct alterations in gastrointestinal cancers.CRC samples exhibited 455 differentially expressed metabolites(234 upregulated and 221 downregulated).Similarly,GC samples exhibited 459 differentially expressed metabolites(251 upregulated and 208 downregulated).Additionally,352 shared metabolites were identified among gastrointestinal cancers.Enrichment analysis highlighted the involvement of these shared metabolites in 10 metabolic pathways.CONCLUSION To some extent,this study revealed the metabolomic characteristics of spleen deficiency and blood stasis toxin(PXYD)syndrome in gastrointestinal cancers.It provides the rationale for the"same treatment for different diseases"approach in PXYD syndrome of gastrointestinal cancers,and for identifying potential metabolomicsbased biomarkers.展开更多
Traditional Chinese medicine （TCM） syndrome, also known as TCM ZHENG or TCM pattern, is an integral and essential part of TCM theory that helps to guide the design of individualized treatments. ATCM syndrome, in ess...Traditional Chinese medicine （TCM） syndrome, also known as TCM ZHENG or TCM pattern, is an integral and essential part of TCM theory that helps to guide the design of individualized treatments. ATCM syndrome, in essence, is a characteristic profile of all clinical manifestations in one patient that can be readily identified by a TCM practitioner. In this article, the authors reviewed the presentations of TCM syndromes in seven common malignancies （liver, lung, gastric, breast, colorectal, pancreatic and esophageal cancers）, the objectivity and the standardization of TCM syndrome differentiation, the evaluation of TCM syndrome modeling in cancer research, and syndrome differentiation-guided TCM treatment of cancers. A better understanding of TCM syndrome theory, as well as its potential biologica basis, may contribute greatly to the clinical TCM diagnosis and the treatment of cancer.展开更多
基金supported by the National Natural Science Foundation of China(32088101)National key Research and Development Program of China(2017YFC1700105,2021YFA1301603).
文摘Objective:To elucidate the biological basis of the heart qi deficiency(HQD)pattern,an in-depth understanding of which is essential for improving clinical herbal therapy.Methods: We predicted and characterized HQD pattern genes using the new strategy,TCM-HIN2Vec,which involves heterogeneous network embedding and transcriptomic experiments.First,a heterogeneous network of traditional Chinese medicine(TCM)patterns was constructed using public databases.Next,we predicted HQD pattern genes using a heterogeneous network-embedding algorithm.We then analyzed the functional characteristics of HQD pattern genes using gene enrichment analysis and examined gene expression levels using RNA-seq.Finally,we identified TCM herbs that demonstrated enriched interactions with HQD pattern genes via herbal enrichment analysis.Results: Our TCM-HIN2Vec strategy revealed that candidate genes associated with HQD pattern were significantly enriched in energy metabolism,signal transduction pathways,and immune processes.Moreover,we found that these candidate genes were significantly differentially expressed in the transcriptional profile of mice model with heart failure with a qi deficiency pattern.Furthermore,herbal enrichment analysis identified TCM herbs that demonstrated enriched interactions with the top 10 candidate genes and could potentially serve as drug candidates for treating HQD.Conclusion: Our results suggested that TCM-HIN2Vec is capable of not only accurately identifying HQD pattern genes,but also deciphering the basis of HQD pattern.Furthermore our finding indicated that TCM-HIN2Vec may be further expanded to develop other patterns,leading to a new approach aimed at elucidating general TCM patterns and developing precision medicine.
基金This work was supported by a grant from the National Basic Research Program of China(973 Program No.2011CB505106).
文摘Objective:To investigate the biological basis of“depression with liver-qi stagnation and spleen deficiency syndrome”.Methods:A digital gene expression profiling method was conducted to explore global changes in the mRNA transcriptome in a rat model of depression with liver-qi stagnation and spleen deficiency syndrome.Real-time quantitative polymerase chain reaction(q-PCR)was performed to verify the five genes most interest based on the Kyoto Encyclopedia of Genes and Genome(KEGG)analysis.Sini San,which disperses stagnated liver qi and strengthens the spleen,was administered to the model rats to observe whether it could reverse these genetic changes in the liver.Results:Forty-six differentially expressed genes were identified.Three of the five genes of most interestdHnf4a,Hnf4g and Cyp1a1dbased on KEGG analysis,were confirmed by realtime q-PCR.Sini San reduced the gene expression changes of Hnf4a,Hnf4g and Cyp1a1 in the rat model.Conclusions:Hnf4a,Hnf4g and Cyp1a1 are involved in“depression with liver-qi stagnation and spleen deficiency syndrome”.These findings indicate that depressed rats with liver-qi stagnation and spleen deficiency syndrome are at risk of liver diseases.Furthermore,our results will inform exploration of the etiology of depression and help in the development of effective therapeutic strategies.
文摘Researchers have discovered that mice instinctively exhibit rescue-like behavior towards anesthetized companions-a finding that suggests a biological basis for prosociality.The study,published in PNAS on April 23,2025,was led by Dr.HU Li from the Institute of Psychology of the Chinese Academy of Sciences(IPCAS)and Dr.CHEN Zhoufeng from Washington University School of Medicine and the Shenzhen Medical Academy of Research and Translation.
基金Supported by the National Key R and D Program of China,No.2022YFC3500200,No.2022YFC3500202 and No.2022YFC3500204Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine,No.ZYYCXTD-C-202208+4 种基金NATCM’s Project of High-Level Construction of Key TCM Disciplines,No.[2023]85Qing Lan Project of Jiangsu Higher Education Institutions,No.[2023]27Postgraduate Research&Practice Innovation Program of Jiangsu Province,No.SJCX22_0706General Project of Universities’Philosophy and Social Science in Jiangsu Province,No.2024SJYB0564Jiangsu Provincial Leading Talents Program in Traditional Chinese Medicine,No.SLJ0314.
文摘BACKGROUND Gastrointestinal cancers are among the most commonly diagnosed cancers globally.Traditional Chinese medicine(TCM)offers distinct advantages in preventing and treating these cancers.AIM To investigate the metabolic basis of a common TCM syndrome in gastrointestinal cancers,exploring underlying metabolic mechanisms and identifying potential METHODS Thirty healthy controls(normal group),30 patients with gastric cancer(GC),and 30 patients with colorectal cancer(CRC)were enrolled in 2023.Plasma metabolic profiles were detected using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry,and pathway enrichment analysis was conducted using the Kyoto Encyclopedia of Genes and Genomes.RESULTS Metabolic profiling revealed distinct alterations in gastrointestinal cancers.CRC samples exhibited 455 differentially expressed metabolites(234 upregulated and 221 downregulated).Similarly,GC samples exhibited 459 differentially expressed metabolites(251 upregulated and 208 downregulated).Additionally,352 shared metabolites were identified among gastrointestinal cancers.Enrichment analysis highlighted the involvement of these shared metabolites in 10 metabolic pathways.CONCLUSION To some extent,this study revealed the metabolomic characteristics of spleen deficiency and blood stasis toxin(PXYD)syndrome in gastrointestinal cancers.It provides the rationale for the"same treatment for different diseases"approach in PXYD syndrome of gastrointestinal cancers,and for identifying potential metabolomicsbased biomarkers.
基金supported by Key Program of National Science Foundation of China(No.81330084)085 First-Class Discipline Construction Innovation Science and Technology Support Project of Shanghai University of TCM(No.085ZY1206)+1 种基金E-institutes of Shanghai Municipal Education Commission(No.E03008)National Natural Science F oundation of China(No.81303102,81303103,81473478,and 81473628)
文摘Traditional Chinese medicine （TCM） syndrome, also known as TCM ZHENG or TCM pattern, is an integral and essential part of TCM theory that helps to guide the design of individualized treatments. ATCM syndrome, in essence, is a characteristic profile of all clinical manifestations in one patient that can be readily identified by a TCM practitioner. In this article, the authors reviewed the presentations of TCM syndromes in seven common malignancies （liver, lung, gastric, breast, colorectal, pancreatic and esophageal cancers）, the objectivity and the standardization of TCM syndrome differentiation, the evaluation of TCM syndrome modeling in cancer research, and syndrome differentiation-guided TCM treatment of cancers. A better understanding of TCM syndrome theory, as well as its potential biologica basis, may contribute greatly to the clinical TCM diagnosis and the treatment of cancer.
