Cell fate determination as a fundamental question in cell biology has been extensively studied at different regulatory levels for many years.However,the mechanisms of multilevel regulation of cell fate determination r...Cell fate determination as a fundamental question in cell biology has been extensively studied at different regulatory levels for many years.However,the mechanisms of multilevel regulation of cell fate determination remain unclear.Recently,we have proposed an Epigenome-Metabolome-Epigenome(E-M-E)signaling cascade model to describe the cross-over cooperation during mouse somatic cell reprogramming.In this review,we summarize the broad roles of E-M-E signaling cascade in different cell biological processes,including cell differentiation and dedifferentiation,cell specialization,cell proliferation,and cell pathologic processes.Precise E-M-E signaling cascades are critical in these cell biological processes,and it is of worth to explore each step of E-M-E signaling cascade.E-M-E signaling cascade model sheds light on and may open a window to explore the mechanisms of multilevel regulation of cell biological processes.展开更多
The pilot scale experimental apparatus and the procedure of the chemical and biological flocculation process to verify the feasibility in treating Shanghai municipal sewage were introduced in this paper. In addition, ...The pilot scale experimental apparatus and the procedure of the chemical and biological flocculation process to verify the feasibility in treating Shanghai municipal sewage were introduced in this paper. In addition, the biological function of the process was discussed. The results of optimal running showed that in the reaction tank, the concentration of mixed liquor suspended solid(MLSS) was 2 g/L, hydraulic retention time(HRT) was 35 min, dosage of liquid polyaluminium chloride(PAC) was 60 mg/L, and the concentration of polyacrylamide(PAM) was 0 5 mg/L. The effluent average concentrations of COD Cr , TP, SS and BOD 5 were 50 mg/L, 0 62 mg/L, 18 mg/L, and 17 mg/L, respectively. These were better than the designed demand. In addition, the existence of biological degradation in this system was proven by several methods. The removal efficiencies of the chemical and biological flocculation process were 20% higher than that of the chemical flocculation process above at the same coagulant dosage. The treatment process under different situations was evaluated on a pilot scale experiment, and the results provided magnificent parameters and optimal condition for future operation of the plant.展开更多
This paper studied the effect of ferric chloride on waste sludge digestion,dewatering and sedimentation under the optimized doses in co-precipitation phosphorus removal process.The experimental results showed that the...This paper studied the effect of ferric chloride on waste sludge digestion,dewatering and sedimentation under the optimized doses in co-precipitation phosphorus removal process.The experimental results showed that the concentration of mixed liquid suspended solid(MLSS) was 2436 mg.L-1 and 2385 mg.L-1 in co-precipitation phosphorus removal process(CPR) and biological phosphorous removal process(BPR),respectively.The sludge reduction ratio for each process was 22.6% and 24.6% in aerobic digestion,and 27.6% and 29.9% in anaerobic digestion,respectively.Due to the addition of chemical to the end of aeration tank,the sludge content of CPR was slightly higher than that of BPR,but the sludge reduction rate for both processes had no distinct difference.The sludge volume index(SVI) and sludge specific resistance of BPR were 126 ml.g-1 and 11.7×1012 m.kg-1,respectively,while those of CPR were only 98 ml.g-1 and 7.1×1012 m.kg-1,indicating that CPR chemical could improve sludge settling and dewatering.展开更多
Two hybrid processes including ozonation-ceramic membrane-biological activated carbon （BAC） （Process A） and ceramic membrane-BAC （Process B） were compared to treat polluted raw water. The performance of hybrid p...Two hybrid processes including ozonation-ceramic membrane-biological activated carbon （BAC） （Process A） and ceramic membrane-BAC （Process B） were compared to treat polluted raw water. The performance of hybrid processes was evaluated with the removal efficiencies of turbidity, ammonia and organic matter. The results indicated that more than 99% of particle count was removed by both hybrid processes and ozonation had no significant effect on its removal. BAC filtration greatly improved the removal of ammonia. Increasing the dissolved oxygen to 30.0 mg/L could lead to a removal of ammonia with concentrations as high as 7.80 mg/L and 8.69 mg/L for Processes A and B, respectively. The average removal efficiencies of total organic carbon and ultraviolet absorbance at 254 nm （UV254, a parameter indicating organic matter with aromatic structure） were 49% and 52% for Process A, 51% and 48% for Process B, respectively. Some organic matter was oxidized by ozone and this resulted in reduced membrane fouling and increased membrane flux by 25%-30%. However, pre-ozonation altered the components of the raw water and affected the microorganisms in the BAC, which may impact the removals of organic matter and nitrite negatively.展开更多
Energy efficiency is closely related to the evolution of biological systems and is important to their information processing. In this work, we calculate the excitation probability of a simple model of a bistable biolo...Energy efficiency is closely related to the evolution of biological systems and is important to their information processing. In this work, we calculate the excitation probability of a simple model of a bistable biological unit in response to pulsatile inputs, and its spontaneous excitation rate due to noise perturbation. Then we analytically calculate the mutual information, energy cost, and energy efficiency of an array of these bistable units. We find that the optimal number of units could maximize this array＇s energy efficiency in encoding pulse inputs, which depends on the fixed energy cost. We conclude that demand for energy efficiency in biological systems may strongly influence the size of these systems under the pressure of natural selection.展开更多
1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions...1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions.Research in cold regions emphasize particularly on the cold-region-characterized physical,chemical and biological processes and their interactions,and on the response of cryosphere to global change and human activities as well as their effects on environment and the acclimatizable strategies;focus on the objects of glacier,snow,river,lake,sea ice,permafrost and seasonal frozen ground,and periglacial geomorphology,etc.;and think much of cold regions engineering and technology.Research in arid regions emphasize particularly on the arid-region-characterized physical.展开更多
1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions...1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions.Research in cold regions emphasize particularly on the cold-region-characterized physical,chemical and biological processes and their interactions,and on the response of cryosphere to global change and human activities as well as their effects on environment and the acclimatizable strategies;focus on the objects of glacier,snow,river,lake,sea ice,permafrost and seasonal frozen ground,and periglacial geomorphology,etc.;and think much of cold regions engineering and technology.Research in arid regions emphasize particularly on the arid-region-characterized physical,chemical and biological processes and their interactions,and on the response of arid regions to global change and human activities as well as their effects on environment and the acclimatizable strategies;focus on the objects of desert,oasis,loess,and aeolian landforms,etc.;and support integrated studies on mountain-desert-oasis system in arid watershed with water resources as masterstroke,so as to provide reliable technological support for water safety,ecology safety and food safety in arid regions.展开更多
1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions...1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions.Research in cold regions emphasize particularly on the cold-region-characterized physical,chemical and biological processes and their interactions,and on the response of cryosphere to global change and human activities as well as their effects on environment and the acclimatizable strategies;focus on the objects of glacier,snow,river,lake,sea ice,permafrost and seasonal frozen ground,and periglacial geomorphology,etc.;and think much of cold regions engineering and technology.展开更多
Fluorescent probes have revolutionized optical imaging and biosensing by enabling real-time visualization, quantification, and tracking of biological processes at molecular and cellular levels. These probes, ranging f...Fluorescent probes have revolutionized optical imaging and biosensing by enabling real-time visualization, quantification, and tracking of biological processes at molecular and cellular levels. These probes, ranging from organic dyes to genetically encoded proteins and nanomaterials, provide unparalleled specificity, sensitivity, and multiplexing capabilities. However, challenges such as brightness, photobleaching, biocompatibility, and emission range continue to drive innovation in probe design and application. This special issue, comprising four review papers and seven original research studies, highlights cutting-edge advancements in fluorescent probe technologies and their transformative roles in super-resolution imaging, in vivo diagnostics, and cancer therapeutics.展开更多
Mesenchymal stem cells are highly regarded for their potential in tissue repair and regenerative medicine due to their multipotency and self-renewal abilities.Recently,mesenchymal stem cells have been redefined as“me...Mesenchymal stem cells are highly regarded for their potential in tissue repair and regenerative medicine due to their multipotency and self-renewal abilities.Recently,mesenchymal stem cells have been redefined as“medical signaling cells,”with their primary biological effects mediated through exosome secretion.These exosomes,which contain lipids,proteins,RNA,and metabolites,are crucial in regulating various biological processes and enhancing regenerative therapies.Exosomes replicate the effects of their parent cells while offering benefits such as reduced side effects,low immunogenicity,excellent biocompatibility,and high drug-loading capacity.Dental stem cells,including those from apical papilla,gingiva,dental pulp,and other sources,are key contributors to exosome-mediated regenerative effects,such as tumor cell apoptosis,neuroprotection,angiogenesis,osteogenesis,and immune modulation.Despite their promise,clinical application of exosomes is limited by challenges in isolation techniques.Current methods face issues of complexity,inefficiency,and insufficient purity,hindering detailed analysis.Recent advancements,such as micro-electromechanical systems,alternating current electroosmosis,and serum-free three-dimensional cell cultures,have improved exosome isolation efficacy.This review synthesizes nearly 200 studies on dental stem cell-derived exosomes,highlighting their potential in treating a wide range of conditions,including periodontal diseases,cancer,neurodegenerative disorders,diabetes,and more.Optimized isolation methods offer a path forward for overcoming current limitations and advancing the clinical use of exosome-based therapies.展开更多
Insulin is a peptide hormone secreted by pancreaticβ-cells,which plays a key role in regulating glucose metabolism and is the only hormone in the body capable of lowering blood glucose level.The development of insuli...Insulin is a peptide hormone secreted by pancreaticβ-cells,which plays a key role in regulating glucose metabolism and is the only hormone in the body capable of lowering blood glucose level.The development of insulin preparations has undergone nearly 100 years of history,from early animal insulin extraction to modern synthetic insulin and insulin analogs,which have greatly advanced the treatment of diabetes.The insulin receptor has a wide distribution in the body,and its activation leads to intracellular signaling mainly through two pathways,PI3K/Akt and Ras/MAPK.Clinically,insulin is crucial in the treatment and management of diabetes and its complications,especially in the cases where oral medications fail to control blood glucose.The role of insulin is not limited to the regulation of blood glucose but has a wide range of functions throughout the body,such as regulation of mitochondrial function and metabolism,the promotion of protein synthesis,adipogenesis,and cellular proliferation.However,insulin overdose may lead to severe hypoglycemia,which,if left untreated,poses the risk of irreversible neurological damage or even fatality.In this paper,we review the history of the development of insulin preparations,the molecular structure of insulin,the biological processes initiated by insulin and insulin deficiency/resistance.The overview of side effects from insulin is also included in this review.We assume that future research could focus on refining insulin analogs for greater therapeutic precision,minimizing side effects,and extending benefits beyond glycemic control.Exploring insulin’s additional effects may unlock potential applications in treating multiple diseases.展开更多
Processive catalysis represents a transformative approach to molecular transformations,wherein a catalyst remains bound to its substrate and undergoes multiple reaction cycles before dissociating.
A basin-wide ocean general circulation model （OGCM） of the Pacific Ocean is employed to estimate the uptake and storage of anthropogenic CO2 using two different simulation approaches. The simulation （named BIO） ma...A basin-wide ocean general circulation model （OGCM） of the Pacific Ocean is employed to estimate the uptake and storage of anthropogenic CO2 using two different simulation approaches. The simulation （named BIO） makes use of a carbon model with biological processes and full thermodynamic equations to calculate surface water partial pressure of CO2, whereas the other simulation （named PTB） makes use of a perturbation approach to calculate surface water partial pressure of anthropogenic CO2. The results from the two simulations agree well with the estimates based on observation data in most important aspects of the vertical distribution as well as the total inventory of anthropogenic carbon. The storage of anthropogenic carbon from BIO is closer to the observation-based estimate than that from PTB. The Revelle factor in 1994 obtained in BIO is generally larger than that obtained in PTB in the whole Pacific, except for the subtropical South Pacific. This, to large extent, leads to the difference in the surface anthropogenic CO2 concentration between the two runs. The relative difference in the annual uptake between the two runs is almost constant during the integration processes after 1850. This is probably not caused by dissolved inorganic carbon （DIC）, but rather by a factor independent of time. In both runs, the rate of change in anthropogenic CO2 fluxes with time is consistent with the rate of change in the growth rate of atmospheric partial pressure of CO2.展开更多
Wallerian degeneration is a subject of major interest in neuroscience. A large number of genes are differentially regulated during the distinct stages of Wallerian degeneration： transcription factor activation, immun...Wallerian degeneration is a subject of major interest in neuroscience. A large number of genes are differentially regulated during the distinct stages of Wallerian degeneration： transcription factor activation, immune response, myelin cell differentiation and dedifferentiation. Although gene expression responses in the distal segment of the sciatic nerve after peripheral nerve injury are known, differences in gene expression between the proximal and distal segments remain unclear. In the present study in rats, we used microarrays to analyze changes in gene expression, biological processes and signaling pathways in the proximal and distal segments of sciatic nerves under- going Wallerian degeneration. More than 6,000 genes were differentially expressed and 20 types of expression tendencies were identified, mainly between proximal and distal segments at 7-14 days after injury. The differentially expressed genes were those involved in cell differentiation, cytokinesis, neuron differentiation, nerve development and axon regeneration. Furthermore, 11 biological processes were represented, related to responses to stimuli, cell apoptosis, inflammato- ry response, immune response, signal transduction, protein kinase activity, and cell proliferation. Using real-time quantitative PCR, western blot analysis and immunohistochemistry, microarray data were verified for four genes： aquaporin-4, interleukin 1 receptor-like 1, matrix metallopro- teinase-12 and periaxin. Our study identifies differential gene expression in the proximal and distal segments of a nerve during Wallerian degeneration, analyzes dynamic biological changes of these genes, and provides a useful platform for the detailed study of nerve injury and repair during Wallerian degeneration.展开更多
Long noncoding RNAs(lncRNAs)participate in a variety of biological processes and diseases.However,the expression and function of lncRNAs after spinal cord injury has not been extensively analyzed.In this study of righ...Long noncoding RNAs(lncRNAs)participate in a variety of biological processes and diseases.However,the expression and function of lncRNAs after spinal cord injury has not been extensively analyzed.In this study of right side hemisection of the spinal cord at T10,we detected the expression of lncRNAs in the proximal tissue of T10 lamina at different time points and found 445 lncRNAs and 6522 mRNA were differentially expressed.We divided the differentially expressed lncRNAs into 26 expression trends and analyzed Profile 25 and Profile 2,the two expression trends with the most significant difference.Our results showed that the expression of 68 lncRNAs in Profile 25 rose first and remained high 3 days post-injury.There were 387 mRNAs co-expressed with the 68 lncRNAs in Profile 25.The co-expression network showed that the co-expressed genes were mainly enriched in cell division,inflammatory response,FcγR-mediated cell phagocytosis signaling pathway,cell cycle and apoptosis.The expression of 56 lncRNAs in Profile2 first declined and remained low after 3 days post-injury.There were 387 mRNAs co-expressed with the 56 lncRNAs in Profile 2.The co-expression network showed that the co-expressed genes were mainly enriched in the chemical synaptic transmission process and in the signaling pathway of neuroactive ligand-receptor interaction.The results provided the expression and regulatory network of the main lncRNAs after spinal cord injury and clarified their co-expressed gene enriched biological processes and signaling pathways.These findings provide a new direction for the clinical treatment of spinal cord injury.展开更多
An anaerobic/oxic membrane bioreactor (A/O MBR) was used for treatment of dyeing wastewater from a woolen mill. COD and color of the wastewater were 54—473 mg/L and 40—400 dilution time (DT) respectively. The ratio ...An anaerobic/oxic membrane bioreactor (A/O MBR) was used for treatment of dyeing wastewater from a woolen mill. COD and color of the wastewater were 54—473 mg/L and 40—400 dilution time (DT) respectively. The ratio of BOD 5/COD was less than 0.13. By the A/O MBR process, the average removal of COD, BOD 5, color and turbidity was 82%, 96%, 71% and 99%, respectively. The average COD, BOD 5, color and turbidity of effluent was 37 mg/L, 0.8 mg/L, 40 DT and 0.44 NUT respectively. The effluent COD met the local standard of reuse water in Beijing, China. The average COD volume load of the anaerobic biological tank was 0.0483 kgCOD/(m 3·d) and that of the aeration tank of the MBR was 0.3589 kgCOD/(m 3·d). The sludge load of the MBR was 0.19 kgCOD/(kg·MLSS·d) on average and the maximum of that was 0.4 kgCOD/(kg·MLSS·d). The flux of the A/O membrane bioreactor could be remained at larger than 50 L/(h·m 2·0.1MPa). The results indicated that A/O membrane bioreactor has technical feasibility for treatment of woolen mill wastewater.展开更多
Olfactory ensheathing cells(OECs) from the olfactory bulb(OB) and the olfactory mucosa(OM) have the capacity to repair nerve injury. However, the difference in the therapeutic effect between OB-derived OECs and OM-der...Olfactory ensheathing cells(OECs) from the olfactory bulb(OB) and the olfactory mucosa(OM) have the capacity to repair nerve injury. However, the difference in the therapeutic effect between OB-derived OECs and OM-derived OECs remains unclear. In this study, we extracted OECs from OB and OM and compared the gene and protein expression profiles of the cells using transcriptomics and non-quantitative proteomics techniques. The results revealed that both OB-derived OECs and OM-derived OECs highly expressed genes and proteins that regulate cell growth, proliferation, apoptosis and vascular endothelial cell regeneration. The differentially expressed genes and proteins of OB-derived OECs play a key role in regulation of nerve regeneration and axon regeneration and extension, transmission of nerve impulses and response to axon injury. The differentially expressed genes and proteins of OM-derived OECs mainly participate in the positive regulation of inflammatory response, defense response, cytokine binding, cell migration and wound healing. These findings suggest that differentially expressed genes and proteins may explain why OB-derived OECs and OM-derived OECs exhibit different therapeutic roles. This study was approved by the Animal Ethics Committee of the General Hospital of Ningxia Medical University(approval No. 2017-073) on February 13, 2017.展开更多
The initial mechanical damage of a spinal cord injury(SCI)triggers a progressive secondary injury cascade,which is a complicated process integrating multiple systems and cells.It is crucial to explore the molecular an...The initial mechanical damage of a spinal cord injury(SCI)triggers a progressive secondary injury cascade,which is a complicated process integrating multiple systems and cells.It is crucial to explore the molecular and biological process alterations that occur after SCI for therapy development.The differences between the rostral and caudal regions around an SCI lesion have received little attention.Here,we analyzed the differentially expressed genes between rostral and caudal sites after injury to determine the biological processes in these two segments after SCI.We identified a set of differentially expressed genes,including Col3a1,Col1a1,Dcn,Fn1,Kcnk3,and Nrg1,between rostral and caudal regions at different time points following SCI.Functional enrichment analysis indicated that these genes were involved in response to mechanical stimulus,blood vessel development,and brain development.We then chose Col3a1,Col1a1,Dcn,Fn1,Kcnk3,and Nrg1 for quantitative real-time PCR and Fn1 for immunostaining validation.Our results indicate alterations in different biological events enriched in the rostral and caudal lesion areas,providing new insights into the pathology of SCI.展开更多
Membrane bioreactor (MBR) used in water and waste water treatment is a developing technique for water pollution control and water reuse. This paper described a membrane bioreactor for treatment of waste water in a pet...Membrane bioreactor (MBR) used in water and waste water treatment is a developing technique for water pollution control and water reuse. This paper described a membrane bioreactor for treatment of waste water in a petrochemical complex. The experimental MBR was a lab scale one composed of an activated sludge bioreactor unit and an ultrafiltration membrane unit. The relationship of COD removal with MLSS and HRT in this MBR was studied. The effects of crossflow velocity, backwash interval and volume of flush liquid on the flux were discussed. The results showed that average removal of COD, oil, SS and turbidity in petrochemical waste water by the MBR was 91%, 86%, 92% and 99%, respectively. The average removal of NH 3 N and total phosphorous was 85% and 82% respectively. A coefficient of COD removal, k , was 0017—0080 L/(mg.d). The membrane flux maintained higher than 60 L/hm 2 bar for 34 days without chemical cleaning when the velocity of crossflow was 35—39 m/s and the backwash interval was 30 minutes and backwash duration at 20 seconds. The results indicated that it is feasible for MBR technology to be used in petrochemical waste water treatment. The treated water could be considered as a source of to make up water for industrial cooling system or to be reused for other purposes.展开更多
基金financially supported by the National Key Research and Development Program of China (2017YFA0106300)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030505)+6 种基金the National Natural Science Foundation projects of China (2017YFA0102900, 2019YFA09004500, 2017YFC1001602, 2016YFA0100300, 2018YFA0107100)the National Natural Science Foundation projects of China (92157202, 32025010, 31801168, 31900614, 31970709, 81901275, 32070729, 32100619, 32170747)the Key Research Program of Frontier Sciences, CAS (QYZDB-SSW-SMC001)International Cooperation Program (154144KYSB20200006)Guangdong Province Science and Technology Program (2020B1212060052, 2018A030313825, 2018GZR110103002, 2020A1515011200, 2020A1515010919, 2020A1515011410, 2021A1515012513)Guangzhou Science and Technology Program (201807010067, 202002030277, 202102021250, 202102020827, 202102080066), Open Research Program of Key Laboratory of Regenerative Biology, CAS (KLRB201907, KLRB202014)CAS Youth Innovation Promotion Association (to Y. W. and K. C.)
文摘Cell fate determination as a fundamental question in cell biology has been extensively studied at different regulatory levels for many years.However,the mechanisms of multilevel regulation of cell fate determination remain unclear.Recently,we have proposed an Epigenome-Metabolome-Epigenome(E-M-E)signaling cascade model to describe the cross-over cooperation during mouse somatic cell reprogramming.In this review,we summarize the broad roles of E-M-E signaling cascade in different cell biological processes,including cell differentiation and dedifferentiation,cell specialization,cell proliferation,and cell pathologic processes.Precise E-M-E signaling cascades are critical in these cell biological processes,and it is of worth to explore each step of E-M-E signaling cascade.E-M-E signaling cascade model sheds light on and may open a window to explore the mechanisms of multilevel regulation of cell biological processes.
文摘The pilot scale experimental apparatus and the procedure of the chemical and biological flocculation process to verify the feasibility in treating Shanghai municipal sewage were introduced in this paper. In addition, the biological function of the process was discussed. The results of optimal running showed that in the reaction tank, the concentration of mixed liquor suspended solid(MLSS) was 2 g/L, hydraulic retention time(HRT) was 35 min, dosage of liquid polyaluminium chloride(PAC) was 60 mg/L, and the concentration of polyacrylamide(PAM) was 0 5 mg/L. The effluent average concentrations of COD Cr , TP, SS and BOD 5 were 50 mg/L, 0 62 mg/L, 18 mg/L, and 17 mg/L, respectively. These were better than the designed demand. In addition, the existence of biological degradation in this system was proven by several methods. The removal efficiencies of the chemical and biological flocculation process were 20% higher than that of the chemical flocculation process above at the same coagulant dosage. The treatment process under different situations was evaluated on a pilot scale experiment, and the results provided magnificent parameters and optimal condition for future operation of the plant.
基金Supported by the Major National Water Sci-Tech Projects of China(2009ZX07210-009)the Department of Environmental Protection of Shandong Province(2006032,2060403)
文摘This paper studied the effect of ferric chloride on waste sludge digestion,dewatering and sedimentation under the optimized doses in co-precipitation phosphorus removal process.The experimental results showed that the concentration of mixed liquid suspended solid(MLSS) was 2436 mg.L-1 and 2385 mg.L-1 in co-precipitation phosphorus removal process(CPR) and biological phosphorous removal process(BPR),respectively.The sludge reduction ratio for each process was 22.6% and 24.6% in aerobic digestion,and 27.6% and 29.9% in anaerobic digestion,respectively.Due to the addition of chemical to the end of aeration tank,the sludge content of CPR was slightly higher than that of BPR,but the sludge reduction rate for both processes had no distinct difference.The sludge volume index(SVI) and sludge specific resistance of BPR were 126 ml.g-1 and 11.7×1012 m.kg-1,respectively,while those of CPR were only 98 ml.g-1 and 7.1×1012 m.kg-1,indicating that CPR chemical could improve sludge settling and dewatering.
基金supported by the National Grand Water Project(No.2008ZX07423-002)the National Natural Science Foundation of China(No.50978170)the Guangdong Provincial Funding(No.2012B030800001)
文摘Two hybrid processes including ozonation-ceramic membrane-biological activated carbon （BAC） （Process A） and ceramic membrane-BAC （Process B） were compared to treat polluted raw water. The performance of hybrid processes was evaluated with the removal efficiencies of turbidity, ammonia and organic matter. The results indicated that more than 99% of particle count was removed by both hybrid processes and ozonation had no significant effect on its removal. BAC filtration greatly improved the removal of ammonia. Increasing the dissolved oxygen to 30.0 mg/L could lead to a removal of ammonia with concentrations as high as 7.80 mg/L and 8.69 mg/L for Processes A and B, respectively. The average removal efficiencies of total organic carbon and ultraviolet absorbance at 254 nm （UV254, a parameter indicating organic matter with aromatic structure） were 49% and 52% for Process A, 51% and 48% for Process B, respectively. Some organic matter was oxidized by ozone and this resulted in reduced membrane fouling and increased membrane flux by 25%-30%. However, pre-ozonation altered the components of the raw water and affected the microorganisms in the BAC, which may impact the removals of organic matter and nitrite negatively.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11105062 and 11265014the Fundamental Research Funds for the Central Universities under Grant Nos LZUJBKY-2011-57 and LZUJBKY-2015-119
文摘Energy efficiency is closely related to the evolution of biological systems and is important to their information processing. In this work, we calculate the excitation probability of a simple model of a bistable biological unit in response to pulsatile inputs, and its spontaneous excitation rate due to noise perturbation. Then we analytically calculate the mutual information, energy cost, and energy efficiency of an array of these bistable units. We find that the optimal number of units could maximize this array＇s energy efficiency in encoding pulse inputs, which depends on the fixed energy cost. We conclude that demand for energy efficiency in biological systems may strongly influence the size of these systems under the pressure of natural selection.
文摘1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions.Research in cold regions emphasize particularly on the cold-region-characterized physical,chemical and biological processes and their interactions,and on the response of cryosphere to global change and human activities as well as their effects on environment and the acclimatizable strategies;focus on the objects of glacier,snow,river,lake,sea ice,permafrost and seasonal frozen ground,and periglacial geomorphology,etc.;and think much of cold regions engineering and technology.Research in arid regions emphasize particularly on the arid-region-characterized physical.
文摘1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions.Research in cold regions emphasize particularly on the cold-region-characterized physical,chemical and biological processes and their interactions,and on the response of cryosphere to global change and human activities as well as their effects on environment and the acclimatizable strategies;focus on the objects of glacier,snow,river,lake,sea ice,permafrost and seasonal frozen ground,and periglacial geomorphology,etc.;and think much of cold regions engineering and technology.Research in arid regions emphasize particularly on the arid-region-characterized physical,chemical and biological processes and their interactions,and on the response of arid regions to global change and human activities as well as their effects on environment and the acclimatizable strategies;focus on the objects of desert,oasis,loess,and aeolian landforms,etc.;and support integrated studies on mountain-desert-oasis system in arid watershed with water resources as masterstroke,so as to provide reliable technological support for water safety,ecology safety and food safety in arid regions.
文摘1.Aims and scope The international journal Research in Cold and Arid Regions,is devoted to publishing the latest research achievements in processes and the patterns of the Earth surface system in cold and arid regions.Research in cold regions emphasize particularly on the cold-region-characterized physical,chemical and biological processes and their interactions,and on the response of cryosphere to global change and human activities as well as their effects on environment and the acclimatizable strategies;focus on the objects of glacier,snow,river,lake,sea ice,permafrost and seasonal frozen ground,and periglacial geomorphology,etc.;and think much of cold regions engineering and technology.
文摘Fluorescent probes have revolutionized optical imaging and biosensing by enabling real-time visualization, quantification, and tracking of biological processes at molecular and cellular levels. These probes, ranging from organic dyes to genetically encoded proteins and nanomaterials, provide unparalleled specificity, sensitivity, and multiplexing capabilities. However, challenges such as brightness, photobleaching, biocompatibility, and emission range continue to drive innovation in probe design and application. This special issue, comprising four review papers and seven original research studies, highlights cutting-edge advancements in fluorescent probe technologies and their transformative roles in super-resolution imaging, in vivo diagnostics, and cancer therapeutics.
文摘Mesenchymal stem cells are highly regarded for their potential in tissue repair and regenerative medicine due to their multipotency and self-renewal abilities.Recently,mesenchymal stem cells have been redefined as“medical signaling cells,”with their primary biological effects mediated through exosome secretion.These exosomes,which contain lipids,proteins,RNA,and metabolites,are crucial in regulating various biological processes and enhancing regenerative therapies.Exosomes replicate the effects of their parent cells while offering benefits such as reduced side effects,low immunogenicity,excellent biocompatibility,and high drug-loading capacity.Dental stem cells,including those from apical papilla,gingiva,dental pulp,and other sources,are key contributors to exosome-mediated regenerative effects,such as tumor cell apoptosis,neuroprotection,angiogenesis,osteogenesis,and immune modulation.Despite their promise,clinical application of exosomes is limited by challenges in isolation techniques.Current methods face issues of complexity,inefficiency,and insufficient purity,hindering detailed analysis.Recent advancements,such as micro-electromechanical systems,alternating current electroosmosis,and serum-free three-dimensional cell cultures,have improved exosome isolation efficacy.This review synthesizes nearly 200 studies on dental stem cell-derived exosomes,highlighting their potential in treating a wide range of conditions,including periodontal diseases,cancer,neurodegenerative disorders,diabetes,and more.Optimized isolation methods offer a path forward for overcoming current limitations and advancing the clinical use of exosome-based therapies.
基金supported by grants from National Natural Science Foundation of China(Grant No.82301577).
文摘Insulin is a peptide hormone secreted by pancreaticβ-cells,which plays a key role in regulating glucose metabolism and is the only hormone in the body capable of lowering blood glucose level.The development of insulin preparations has undergone nearly 100 years of history,from early animal insulin extraction to modern synthetic insulin and insulin analogs,which have greatly advanced the treatment of diabetes.The insulin receptor has a wide distribution in the body,and its activation leads to intracellular signaling mainly through two pathways,PI3K/Akt and Ras/MAPK.Clinically,insulin is crucial in the treatment and management of diabetes and its complications,especially in the cases where oral medications fail to control blood glucose.The role of insulin is not limited to the regulation of blood glucose but has a wide range of functions throughout the body,such as regulation of mitochondrial function and metabolism,the promotion of protein synthesis,adipogenesis,and cellular proliferation.However,insulin overdose may lead to severe hypoglycemia,which,if left untreated,poses the risk of irreversible neurological damage or even fatality.In this paper,we review the history of the development of insulin preparations,the molecular structure of insulin,the biological processes initiated by insulin and insulin deficiency/resistance.The overview of side effects from insulin is also included in this review.We assume that future research could focus on refining insulin analogs for greater therapeutic precision,minimizing side effects,and extending benefits beyond glycemic control.Exploring insulin’s additional effects may unlock potential applications in treating multiple diseases.
基金supported by National Natural Science Foundation of China(No.23CAA01015)China Petroleum&Chemical Corporation Seed Plan(No.2550042076).
文摘Processive catalysis represents a transformative approach to molecular transformations,wherein a catalyst remains bound to its substrate and undergoes multiple reaction cycles before dissociating.
基金supported by the Research and Development Special Fund for Public Welfare Industry(Meteorology)(Grant No.2008416022)the National Natural Science Foundation of China(GrantNo.40730106)+2 种基金the National Basic Research Program of China("973program",Grant No.2010CB951802)the Ocean Public Welfare Scientific Research ProjectState Oceanic Administration of the People's Republic of China(Grant No.200905012-4)
文摘A basin-wide ocean general circulation model （OGCM） of the Pacific Ocean is employed to estimate the uptake and storage of anthropogenic CO2 using two different simulation approaches. The simulation （named BIO） makes use of a carbon model with biological processes and full thermodynamic equations to calculate surface water partial pressure of CO2, whereas the other simulation （named PTB） makes use of a perturbation approach to calculate surface water partial pressure of anthropogenic CO2. The results from the two simulations agree well with the estimates based on observation data in most important aspects of the vertical distribution as well as the total inventory of anthropogenic carbon. The storage of anthropogenic carbon from BIO is closer to the observation-based estimate than that from PTB. The Revelle factor in 1994 obtained in BIO is generally larger than that obtained in PTB in the whole Pacific, except for the subtropical South Pacific. This, to large extent, leads to the difference in the surface anthropogenic CO2 concentration between the two runs. The relative difference in the annual uptake between the two runs is almost constant during the integration processes after 1850. This is probably not caused by dissolved inorganic carbon （DIC）, but rather by a factor independent of time. In both runs, the rate of change in anthropogenic CO2 fluxes with time is consistent with the rate of change in the growth rate of atmospheric partial pressure of CO2.
基金supported by the National Natural Science Foundation of China,No.81370982,31170946Key Program,Grant No.81130080the Priority Academic Program Development of Jiangsu Higher Education Institutions in China
文摘Wallerian degeneration is a subject of major interest in neuroscience. A large number of genes are differentially regulated during the distinct stages of Wallerian degeneration： transcription factor activation, immune response, myelin cell differentiation and dedifferentiation. Although gene expression responses in the distal segment of the sciatic nerve after peripheral nerve injury are known, differences in gene expression between the proximal and distal segments remain unclear. In the present study in rats, we used microarrays to analyze changes in gene expression, biological processes and signaling pathways in the proximal and distal segments of sciatic nerves under- going Wallerian degeneration. More than 6,000 genes were differentially expressed and 20 types of expression tendencies were identified, mainly between proximal and distal segments at 7-14 days after injury. The differentially expressed genes were those involved in cell differentiation, cytokinesis, neuron differentiation, nerve development and axon regeneration. Furthermore, 11 biological processes were represented, related to responses to stimuli, cell apoptosis, inflammato- ry response, immune response, signal transduction, protein kinase activity, and cell proliferation. Using real-time quantitative PCR, western blot analysis and immunohistochemistry, microarray data were verified for four genes： aquaporin-4, interleukin 1 receptor-like 1, matrix metallopro- teinase-12 and periaxin. Our study identifies differential gene expression in the proximal and distal segments of a nerve during Wallerian degeneration, analyzes dynamic biological changes of these genes, and provides a useful platform for the detailed study of nerve injury and repair during Wallerian degeneration.
文摘Long noncoding RNAs(lncRNAs)participate in a variety of biological processes and diseases.However,the expression and function of lncRNAs after spinal cord injury has not been extensively analyzed.In this study of right side hemisection of the spinal cord at T10,we detected the expression of lncRNAs in the proximal tissue of T10 lamina at different time points and found 445 lncRNAs and 6522 mRNA were differentially expressed.We divided the differentially expressed lncRNAs into 26 expression trends and analyzed Profile 25 and Profile 2,the two expression trends with the most significant difference.Our results showed that the expression of 68 lncRNAs in Profile 25 rose first and remained high 3 days post-injury.There were 387 mRNAs co-expressed with the 68 lncRNAs in Profile 25.The co-expression network showed that the co-expressed genes were mainly enriched in cell division,inflammatory response,FcγR-mediated cell phagocytosis signaling pathway,cell cycle and apoptosis.The expression of 56 lncRNAs in Profile2 first declined and remained low after 3 days post-injury.There were 387 mRNAs co-expressed with the 56 lncRNAs in Profile 2.The co-expression network showed that the co-expressed genes were mainly enriched in the chemical synaptic transmission process and in the signaling pathway of neuroactive ligand-receptor interaction.The results provided the expression and regulatory network of the main lncRNAs after spinal cord injury and clarified their co-expressed gene enriched biological processes and signaling pathways.These findings provide a new direction for the clinical treatment of spinal cord injury.
文摘An anaerobic/oxic membrane bioreactor (A/O MBR) was used for treatment of dyeing wastewater from a woolen mill. COD and color of the wastewater were 54—473 mg/L and 40—400 dilution time (DT) respectively. The ratio of BOD 5/COD was less than 0.13. By the A/O MBR process, the average removal of COD, BOD 5, color and turbidity was 82%, 96%, 71% and 99%, respectively. The average COD, BOD 5, color and turbidity of effluent was 37 mg/L, 0.8 mg/L, 40 DT and 0.44 NUT respectively. The effluent COD met the local standard of reuse water in Beijing, China. The average COD volume load of the anaerobic biological tank was 0.0483 kgCOD/(m 3·d) and that of the aeration tank of the MBR was 0.3589 kgCOD/(m 3·d). The sludge load of the MBR was 0.19 kgCOD/(kg·MLSS·d) on average and the maximum of that was 0.4 kgCOD/(kg·MLSS·d). The flux of the A/O membrane bioreactor could be remained at larger than 50 L/(h·m 2·0.1MPa). The results indicated that A/O membrane bioreactor has technical feasibility for treatment of woolen mill wastewater.
基金supported by Key Research Projects of Ningxia Hui Autonomous Region of China,No.2018BCG01002(to HCX)the Natural Science Foundation of Ningxia Hui Autonomous Region of China,No.NZ17150(to PY)。
文摘Olfactory ensheathing cells(OECs) from the olfactory bulb(OB) and the olfactory mucosa(OM) have the capacity to repair nerve injury. However, the difference in the therapeutic effect between OB-derived OECs and OM-derived OECs remains unclear. In this study, we extracted OECs from OB and OM and compared the gene and protein expression profiles of the cells using transcriptomics and non-quantitative proteomics techniques. The results revealed that both OB-derived OECs and OM-derived OECs highly expressed genes and proteins that regulate cell growth, proliferation, apoptosis and vascular endothelial cell regeneration. The differentially expressed genes and proteins of OB-derived OECs play a key role in regulation of nerve regeneration and axon regeneration and extension, transmission of nerve impulses and response to axon injury. The differentially expressed genes and proteins of OM-derived OECs mainly participate in the positive regulation of inflammatory response, defense response, cytokine binding, cell migration and wound healing. These findings suggest that differentially expressed genes and proteins may explain why OB-derived OECs and OM-derived OECs exhibit different therapeutic roles. This study was approved by the Animal Ethics Committee of the General Hospital of Ningxia Medical University(approval No. 2017-073) on February 13, 2017.
基金supported by Postgraduate Research&Practice Innovation Program of Jiangsu Province,No.KYCX-2065(to XMC).
文摘The initial mechanical damage of a spinal cord injury(SCI)triggers a progressive secondary injury cascade,which is a complicated process integrating multiple systems and cells.It is crucial to explore the molecular and biological process alterations that occur after SCI for therapy development.The differences between the rostral and caudal regions around an SCI lesion have received little attention.Here,we analyzed the differentially expressed genes between rostral and caudal sites after injury to determine the biological processes in these two segments after SCI.We identified a set of differentially expressed genes,including Col3a1,Col1a1,Dcn,Fn1,Kcnk3,and Nrg1,between rostral and caudal regions at different time points following SCI.Functional enrichment analysis indicated that these genes were involved in response to mechanical stimulus,blood vessel development,and brain development.We then chose Col3a1,Col1a1,Dcn,Fn1,Kcnk3,and Nrg1 for quantitative real-time PCR and Fn1 for immunostaining validation.Our results indicate alterations in different biological events enriched in the rostral and caudal lesion areas,providing new insights into the pathology of SCI.
文摘Membrane bioreactor (MBR) used in water and waste water treatment is a developing technique for water pollution control and water reuse. This paper described a membrane bioreactor for treatment of waste water in a petrochemical complex. The experimental MBR was a lab scale one composed of an activated sludge bioreactor unit and an ultrafiltration membrane unit. The relationship of COD removal with MLSS and HRT in this MBR was studied. The effects of crossflow velocity, backwash interval and volume of flush liquid on the flux were discussed. The results showed that average removal of COD, oil, SS and turbidity in petrochemical waste water by the MBR was 91%, 86%, 92% and 99%, respectively. The average removal of NH 3 N and total phosphorous was 85% and 82% respectively. A coefficient of COD removal, k , was 0017—0080 L/(mg.d). The membrane flux maintained higher than 60 L/hm 2 bar for 34 days without chemical cleaning when the velocity of crossflow was 35—39 m/s and the backwash interval was 30 minutes and backwash duration at 20 seconds. The results indicated that it is feasible for MBR technology to be used in petrochemical waste water treatment. The treated water could be considered as a source of to make up water for industrial cooling system or to be reused for other purposes.
