AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma ...AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma cell line PANC-1 and explore their clinical significance.METHODS:The immunohistochemistry and Western blot were used to determine the expression of TLR9 protein in pancreatic cancer tissues,and immunofluorescence staining was performed to detect the TLR9 protein expression in pancreatic carcinoma cell line PANC-1.To assess the effects of CPG ODN2216 on the invasive property of Panc-1 cells,in vitro cell adhesion,wound-healing scrape,and invasion and cell colony formation were evaluated.RESULTS:TLR9 was highly expressed in pancreaticcancer tissues and PANC-1 cells.The percentage of positive cells expressing TLR9 protein in human pancreatic tissues,paracancerous tissues and normal tissues were 73.3%,33.3% and 20.0%,respectively,and the protein expression level of TLR9 was gradually descending(P < 0.05).In vitro tests in wound-healing scrape,cell adhesion,colony formation and matrigel invasion showed that the adhesion and motility of PANC-1 cells in CPG ODN 2216 treatment group were signif icantly lower than in the control group(P < 0.05).The cell growth assay showed that the proliferative ability of PANC-1 cells in treatment group was significantly decreased and CPG ODN2216 had an inhibitive effect in the growth of Panc-1 cells in a dose and time-dependent manner(P < 0.05).CONCLUSION:The gene of TLR9 is correlated with the invasive and metastatic potential of human pancreatic carcinoma,and CPG ODN2216 induces the inhibition of migration and invasion of Panc-1 cells.展开更多
AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformati...AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformation(non-HGT)primary cells cultured by non-highgrade transformation tissue in proliferation,metastasis,drug susceptibility,and genes.METHODS:LACC-HGT primary cells were established by tissue block culture,and the 4^(th)to 10^(th)generation primary cells were selected as research objects.The cells were preliminarily identified by immunofluorescent staining.The differences between non-HGT and LACC-HGT primary cells in terms of proliferation,metastasis,and drug susceptibility were compared by cell counting kit-8(CCK-8)assay,wound healing,and drug sensitivity experiments.Differentially expressed genes were screened using mRNA array.Gene expression was analyzed using real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS:LACC-HGT primary cells were successfully cultured by tissue block culture.Immunofluorescence staining results showed that cytokeratin(CK)and CK7 expression levels were positive in LACC-HGT primary cells.CCK-8 results showed that the proliferation ability of LACCHGT cells was significantly higher than that of non-HGT cells.Wound healing experiment showed that the migration ability of LACC-HGT cells was significantly higher than that of non-HGT cells.LACC-HGT cells were also less sensitive to cisplatin and paclitaxel than non-HGT cells.Compared with non-HGT cells,9566 differentially expressed genes were found in LACC-HGT primary cells,of which 5162 were upregulated and 4404 were down-regulated.The expression of N-acetylneuraminate pyruvate lyase(NPL),MARVEL domain containing 3(MARVELD3),syntabulin(SYBU),and allograft inflammatory factor 1(AIF1)was higher in LACCHGT cells than in non-HGT cells,whereas that of periostin(POSTN)was lower.CONCLUSION:LACC-HGT primary cells have faster proliferation,stronger migration ability,and poorer sensitivity to chemotherapy drugs than non-HGT primary cells.The expression of mRNAs in non-HGT and LACC-HGT primary cells are significantly different.These features are speculated to be the reasons why high-grade transformation tissues exhibit higher malignant degree and poorer prognosis than their counterparts.展开更多
Dopamine agonists （DA） are a first-line therapy for prolactinomas （PA）. However, nearly 10% of prolactinomas do not respond to DA therapy. A considerable number of studies have shown that estrogen plays an importa...Dopamine agonists （DA） are a first-line therapy for prolactinomas （PA）. However, nearly 10% of prolactinomas do not respond to DA therapy. A considerable number of studies have shown that estrogen plays an important role in the development of prolactinomas. However, the expression of estrogen receptors （ER） in prolactinomas has not been fully explored. Accordingly, we examined the levels of ESR1 and its subtypes A5-DeI-ESR1 and ESR2 mRNA in prolactinomas. In the present study,展开更多
<strong><em>Background. </em></strong>The active ingredient curcumin of traditional Chinese medicine was selected as the research object to investigate the possible mechanism of breast cancer m...<strong><em>Background. </em></strong>The active ingredient curcumin of traditional Chinese medicine was selected as the research object to investigate the possible mechanism of breast cancer metastatic bone pain in mouse walker 256 cells and the effect of curcumin on the NF-κB/TNF-α pathway in order to provide a new idea for clinical treatment of breast cancer metastatic bone pain. <strong><em>Methods.</em></strong> By establishing an animal model of breast cancer bone metastasis in walker 256 cells, the biological behavior of nude mice was observed on the 8th day after successful modeling. Meanwhile, the low dose group, middle dose group and high dose group of mice were given 15 mg/kg, 25 mg/kg, 50 mg/kg of curcumin solution intraperitoneally in 21 days, and the right cavity bone and spinal cord distended in mice (L4-L6) tissues were used to detect related factors, Immunohistochemical method was used to detect c-fos in spinal cord. Expression levels of RANK, NF-κB and TNF-α were detected by RT-PCR and Western blot. Meanwhile, serum levels of Cox2, il-6, leukotriene and PGE2 were detected.<strong><em> Results. </em></strong>Observing the biological behavior index of nude mice, we found that the mechanical pain and thermal pain threshold decreased (p < 0.05), and the cold pain and spontaneous pain scores increased significantly (p < 0.05). After group study, the expression of c-fos in the cancer pain model group was significantly higher than that in the normal control group (p < 0.05), and with the increase of curcumin dose, the expression of c-fos in the high dose group was significantly lower than that in the solvent model group (p < 0.05). The expression of RANK, NF-κB, TNF-α was higher than that of the normal control group and decreased gradually with the increase of curcumin dose, among which the expression of high dose group was significantly lower than that of solvent group (p < 0.05). RANK, NF-κB, TNF-α protein expression was higher than that of normal control group and gradually decreased with the increase of curcumin dose. The levels of Cox2, IL-6, leukotriene and PGE2 in serum decreased with the increase of curcumin dose, and the high dose group decreased significantly (p < 0.05). <em><strong>Conclusions. </strong></em>On the 8th day after the success of the animal model of breast cancer bone metastasis in Walker 256 cells, abnormal biological behaviors such as heat pain, cold pain sensation and spontaneous hyperalgesia were observed. Further studies have found that the increased expression of rank on osteoclasts induced up-regulated expression of NF-κB and c-fos, induced expression of TNF-α gene, and could induce synthesis and release of leukotriene, PGE2 through direct activation of cyclooxygenase, inflammatory media IL-6 cascade reaction, resulting in pathological pain and hypersensitivity. Traditional Chinese medicine active ingredient curcumin could reduce RANK expression of osteoclast, inhibit cell NF-κB and spinal cord c-fos activity, reduce TNF-α expression, inhibit Cox2 activity, and reduce the synthesis and release of inflammatory factors leukotriene and PGE2, thus exerting its analgesic effect, which provides new ideas and methods for clinical treatment of metastatic bone pain in breast cancer.展开更多
BACKGROUND: The high level of matrix metalloproteinase 9(MMP9) is thought to slow down the healing of diabetic foot ulcers. Whether it can influence the biological behaviors of skin fi broblasts and affect wound heali...BACKGROUND: The high level of matrix metalloproteinase 9(MMP9) is thought to slow down the healing of diabetic foot ulcers. Whether it can influence the biological behaviors of skin fi broblasts and affect wound healing is still unclear. The present study aimed to observe changes in the biological behaviors of rat dermal fi broblasts induced by high expression of MMP9 and to clarify the possible mechanisms of wound healing for diabetic foot.METHODS: A cell model of skin f ibroblast with high expression of MMP9 was established by coculture of high glucose(22.0 mmol/L) and homocysteine(100 μmol/L). A control group was incubated with normal glucose(5.5 mmol/L). Realtime PCR, ELISA and gelatin zymography were used to detect the MMP9 mRNA, protein expression and activity of MMP9. Flow cytometry, CCK-8, ELISA assay, scratch test and transwell were used to detect cell proliferation, viability, collagen(hydroxyproline) secretion, horizontal migration and vertical migration of cells. The data were expressed as mean±SD. P value less than 0.05 was considered statistically signif icant.RESULTS: The expression of MMP9 mRNA, protein levels and the activity of MMP9 were much higher in the high MMP9 group than in the control group(7.05±1.02 vs. 1.00±0.00, 206.9±33.6 pg/mL vs. 40.4±5.9 pg/mL, and 1.47±0.13 vs. 0.57±0.12, respectively, P<0.01). The proportion of S-phase cells, proliferation index, cell viability, collagen(hydroxyproline) secretion, horizontal migration rate and the number of vertical migration cells were lower in the high MMP9 group than in the control group(P<0.01).CONCLUSION: Fibroblasts with a high expression of MMP9 decreased proliferation, activity, secretion and migration of collagens, suggesting that MMP9 may inhibit the biological behaviors of fi broblasts.展开更多
BACKGROUND Insulin-like growth factor-1 receptor(IGF-1R)is over-expressed in hepatocellular carcinoma(HCC).However,the relationship between IGF-1R activation and HCC progression remains unidentified.AIM To investigate...BACKGROUND Insulin-like growth factor-1 receptor(IGF-1R)is over-expressed in hepatocellular carcinoma(HCC).However,the relationship between IGF-1R activation and HCC progression remains unidentified.AIM To investigate the effects of editing IGF-1R on the biological features of HCC cells.METHODS Immunohistochemistry analyzed the expressions of IGF-1R and P-glyco protein(P-gp)in HCC tissues and their distal non-cancerous tissues(non-Ca).IGF-1R was edited with Crispr/Cas9 system,screened specific sg RNAs,and then transfected into Hep G2 cells.CCK-8,scratch wound test detected cell proliferation,migration,invasion and transwell assays,respectively.Alterations of IGF-1R and P-gp were confirmed by Western blotting.Alterations of anti-cancer drug IC_(50)values were analyzed at the cell level.RESULTS The positive rates of IGF-1R(93.6%,χ~2=63.947)or P-gp(88.2%,χ~2=58.448)were significantly higher(P<0.001)in the HCC group than those(36.6%in IGF-1R or 26.9%in P-gp)in the non-Ca group.They were positively correlated between high IGF-1R and P-gp expression,and they were associated with hepatitis B virus infection and vascular invasion of HCC.Abnormal expressions of circulating IGF-1R and P-gp were confirmed and associated with HCC progression.Biological feature alterations of HCC cells transfected with specific sg RNA showed IGF-1R expression down-regulation,cell proliferation inhibition,cell invasion or migration potential decreasing,and enhancing susceptibility of Hep G2 cells to anti-cancer drugs.CONCLUSION Edited oncogenic IGF-1R was useful to inhibit biological behaviors of Hep G2 cells.展开更多
OBJECTIVE To observe the effects expression and activation on biological cancer stem cells. of blocking CD133 gene characteristic of the colon METHODS CD133＋ colon cancer stem cells （CCSCs） were separated from EpCA...OBJECTIVE To observe the effects expression and activation on biological cancer stem cells. of blocking CD133 gene characteristic of the colon METHODS CD133＋ colon cancer stem cells （CCSCs） were separated from EpCAMhigh CD44＋ CCSCs through fluorescenceactivated cell sorting （FACS）. The proliferation, the capability of spherical cell formation, neoplasia, and the expression of ABCG2 mRNA of CD133＋ CCSCs were observed after the CD133＋ CCSCs were infected with LV-CD133shRNA. CD133 negative cells were isolated from EpCAMhigh CD44＋ CCSCs with FACS, and the CD133 proteins in CD133- cells were detected with Western blot. RESULTS CD133＋ CCSCs were isolated from EpCAMhigh CD44＋ CCSCs using FACS, and they accounted for 89.2% in the stem cells. In the experimental group, after the CD133＋ CCSCs were knocked down by LV-CD133shRNA RNAi, the growth pattern of the cells in the stem cell culture changed into adherent growth from suspended growth, and couldn＇t generate spherical cells. Results of MTT assay showed that the CD133＋ CCSCs infected with LV-CD133shRNA grew slowly, compared to the cells in the control groups. There was a decrease in the cloning efficiency. The infected cells were transplanted into the BALB/c nude mice. During the observation, no neoplasia was found in the CD133＋ cells infected with LV-CD133shRNA. The level of ABCG2 mRNA expression was lowered greatly （P 〈 0.01）. CD133- cells were obtained from the EpCAMhigh CD44＋ CCSCs using FACS, in which the expression of CD133 protein was positive. CONCLUSION CD133 retains the biological characteristics of the colon cancer stem cells.展开更多
Objective:To investigate the effect of lentiviral stable high expression of circLIFR on the biological behavior of Hep3B hepatocellular carcinoma(HCC)cells.Methods:Hep3B cell lines were infected with lentiviral packag...Objective:To investigate the effect of lentiviral stable high expression of circLIFR on the biological behavior of Hep3B hepatocellular carcinoma(HCC)cells.Methods:Hep3B cell lines were infected with lentiviral packaging of circLIFR expression plasmids to construct stable circLIFR high expression HCC cells.The lentivirus infected with circLIFR high expression sequence was used as the circLIFR high expression group,the lentivirus infected with circLIFR high expression empty vector sequence was used as the negative control group,and the uninfected group was used as the blank control group.After that,circLIFR expression levels were detected by qPCR,and the back splice sites were identified by Sanger sequencing.The cell viability was examined by cell proliferation kit and invasive ability was determined by Transwell assay.Results:The qPCR and Sanger sequencing showed that the stable circLIFR expression of Hep3B cells was successfully established.The circLIFR high expression group had better cell proliferation viability than the negative control and blank control groups,and the differences were statistically significant(P<0.05).The number of cells crossing Matrigel gel in the negative control and blank control groups was(270.8±18.9)and(266.2±17.6),respectively,while the number of cells crossing Matrigel gel in the circLIFR high expression group was(396.6±32.9),and the differences were statistically significant(P<0.05).Conclusion:High circLIFR expression considerably promotes the proliferation and invasive ability of Hep3B cells.展开更多
Obiective:To investigate the effects of Xiaoxianxiong Tang on the proliferation,invasion,apoptosis,and other cell biological behaviors of non-small cell lung cancer A549 cells.Methods:Human lung adenocarcinoma A549 ce...Obiective:To investigate the effects of Xiaoxianxiong Tang on the proliferation,invasion,apoptosis,and other cell biological behaviors of non-small cell lung cancer A549 cells.Methods:Human lung adenocarcinoma A549 cells were cultured in vitro,and the IC50 concentration and effective time of administration of Xiaoxianxiong Tang were determined by the CCK-8 assay to detect the inhibitory effect of Xiaoxianxiong Tang on A549 cells proliferation.The effect of the Xiaoxianxiong Tang on apoptosis was determined by flow cytometry;the apoptosis-related protein was detected via Western blot;the metastasis-related protein mRNA was detected by RT-PCR.Results:Xiaoxianxiong Tang significantly inhibited the proliferation viability,the invasive ability,and the clonogenic ability of A549 cells compared with the control group(P<0.001).Moreover,Xiaoxianxiong Tang significantly promoted the apoptosis of A549 cells(P<0.001).Xiaoxianxiong Tang significantly up-regulated Bax and down-regulated Bcl2 expression in A549 cells compared with the control group(P<0.01).The mRNA expression of MMP2 and MMP9 was significantly down-regulated by Xiaoxianxiong Tang compared with the control group(P<0.05).Conclusion:Xiaoxianxiong Tang has the effect of regulating the biological behavior of A549 cells,and Xiaoxianxiong Tang significantly inhibites the proliferation viability,colony formation,and invasion ability of lung cancer A549 cells.展开更多
Objective To characterize and compare the different biological behaviors of two novel human osteosarcoma cell lines,Zos and Zos-M,established respectively from the primary site and the skip metastasis of an osteosarco...Objective To characterize and compare the different biological behaviors of two novel human osteosarcoma cell lines,Zos and Zos-M,established respectively from the primary site and the skip metastasis of an osteosarcoma patient.Methods Two展开更多
Background:Bladder cancer(BLCA)is the most prevalent malignancy in the urinary tract system,while ST3GAL5 is a protein-coding gene that catalyzes the formation of ganglioside monosialodihexosylganglioside 3(GM3)syntha...Background:Bladder cancer(BLCA)is the most prevalent malignancy in the urinary tract system,while ST3GAL5 is a protein-coding gene that catalyzes the formation of ganglioside monosialodihexosylganglioside 3(GM3)synthase.GM3 synthase has been reported to significantly influence the malignant process of various cancers.However,the expression profile and functional role of ST3GAL5 specifucally in BLCA remain to be elucidated.Methods:In order to investigate the relationship between ST3GAL5 expression and malignant biological behavior and prognosis in BLCA.The mRNA expression of ST3GAL5 and clinicopathological characteristics in BLCA were firstly evaluated by public databases.Next,immunohistochemical staining was performed to analyze the protein expression of ST3GAL5 in BLCA and paracancerous tissues,as well as the expression of various types of malignant biological behavior.Subsequently,the gene set enrichment analyses(GSEA)were performed for ST3GAL5 and all correlated genes in BLCA by sorting Pearson's Correlation Coefficient.GSEA was also used to validate the pathways affected by the different expression levels of ST3GAL5 in BLCA patients.Results:The mRNA and protein expression of ST3GAL5 in BLCA were significantly higher in low-grade and non-muscle-invasive BLCA(p<0.05).The results from bioinformatics databases indicated that upregulation of ST3GAL5 have a lower grade,lower pathological stage,less susceptibility to lymphatic metastasis,and lower mortality rates.Kaplan–Meier survival analysis demonstrated that upregulation of ST3GAL5 was associated with better survival in BLCA(p<0.05).Conclusion:Upregulation of ST3GAL5 may be related to tumor suppression in BLCA,and may be a potential prognostic and therapeutic marker for BLCA.展开更多
Mitochondria play a crucial role as organelles,managing several physiological processes such as redox balance,cell metabolism,and energy synthesis.Initially,the assumption was that mitochondria primarily resided in th...Mitochondria play a crucial role as organelles,managing several physiological processes such as redox balance,cell metabolism,and energy synthesis.Initially,the assumption was that mitochondria primarily resided in the host cells and could exclusively transmit from oocytes to offspring by a mechanism known as vertical inheritance of mitochondria.Recent scholarly works,however,suggest that certain cell types transmit their mitochondria to other developmental cell types via a mechanism referred to as intercellular or horizontal mitochondrial transfer.This review details the process of which mitochondria are transferred across cells and explains the impact of mitochondrial transfer between cells on the efficacy and functionality of cancer cells in various cancer forms.Specifically,we review the role of mitochondria transfer in regulating cellular metabolism restoration,excess reactive oxygen species(ROS)generation,proliferation,invasion,metastasis,mitophagy activation,mitochondrial DNA(mtDNA)inheritance,immune system modulation and therapeutic resistance in cancer.Additionally,we highlight the possibility of using intercellular mitochondria transfer as a therapeutic approach to treat cancer and enhance the efficacy of cancer treatments.展开更多
Objective:To develop an engineered therapeutic tool based on miR-214,analyze the effects of regulating miR-214 on the biological behavior of cervical cancer cells,and explore its underlying mechanisms.Methods:Two type...Objective:To develop an engineered therapeutic tool based on miR-214,analyze the effects of regulating miR-214 on the biological behavior of cervical cancer cells,and explore its underlying mechanisms.Methods:Two types of miR-214 nano-paper carriers were self-designed according to different ratios.The stability,cellular uptake rate,and targeting ability of different carriers were tested.Human cervical epithelial cells and C6330 He La human cervical cancer cells were cultured.The human cervical epithelial cells in the logarithmic growth phase were used as the blank group.The C6330 He La human cervical cancer cells in the logarithmic growth phase were randomly divided into five groups.Cells from each group were seeded in cell culture plates.The blank group and the model group received no treatment.The miR-214 inhibition group was transfected with miR-214 inhibitor.The miR-214 activation group was delivered miR-214 by high-performance nano-lipid carriers.The MEK3 knockout group was transfected with miR-214 mimic after MEK3 gene knockout.The relative expression levels of miR-214 in each group of cells at 24,48,and 72 h after transfection were detected by real-time fluorescence quantitative PCR.The cell proliferation ability was evaluated by measuring the absorbance(OD)value using the MTT colorimetric method.The cell migration ability was evaluated by the cell scratch assay,and the cell apoptosis rate was evaluated by cell apoptosis detection.Results:The particle size of the 4∶5∶1 ratio carrier was significantly smaller than that of the 7∶2∶1 ratio carrier,while the Zeta potential,encapsulation efficiency,fluorescence intensity,and relative expression level of miR-214 were significantly higher than those of the 7∶2∶1 ratio carrier(P<0.05).After miR-214 inhibition and activation treatment in cervical cancer cells,the relative expression levels of miR-214 in cervical cancer cells decreased and increased respectively,and MEK3 knockout did not affect the change in the relative expression level of miR-214.The OD value of the miR-214 inhibition group was significantly higher than that of the model group,and the OD values of the miR-214 activation group and the MEK3 knockout group were significantly lower than that of the model group(P<0.05).The OD value of the miR-214 activation group was significantly lower than that of the MEK3 knockout group(P<0.05).After miR-214 inhibition treatment in cervical cancer cells,the cell migration ability was significantly enhanced,and the cell apoptosis rate was significantly reduced.After miR-214 activation treatment,the cell migration ability was significantly reduced,and the cell apoptosis rate was significantly increased.However,after knocking out the MEK3 gene,the cell proliferation,migration,and apoptosis abilities all decreased.Conclusion:Activating miR-214 can positively affect the biological behavior of cervical cancer cells.In this study,a nano-paper carrier was self-designed through engineering means,which can achieve efficient delivery of miR-214 and provide a new technological platform for the treatment of cervical cancer.展开更多
Our previous studies revealed that second malevibration signal (SMVS) restrained the matingbehavior of N. lugens, the influences of threebiological features (density, age, and wingform) on SMVS’s inhibitory effect we...Our previous studies revealed that second malevibration signal (SMVS) restrained the matingbehavior of N. lugens, the influences of threebiological features (density, age, and wingform) on SMVS’s inhibitory effect were hereinstudied by playing back its record. The dura-tion of playback was 4 h. Except otherwisestatement, N. lugens tested were virginmacropterous males and females aged 4-6 d af-ter emergence, and the density was 5 pairs (5females and 5 males) of N. lugens per cage (4cm in diameter and 8 cm in height). The in-hibitory effect of SMVS was evaluated usingmating rate (i. e. the rate of females withspermatophore). The results were as follows:展开更多
AIM: To explore the biological behavior of gastric carcinoma micrometastasis (MM) with a marker of cytokeratin 18 (CK18) and to evaluate the clinical stage of gastric carcinoma and its prognosis. METHODS: Reverse tran...AIM: To explore the biological behavior of gastric carcinoma micrometastasis (MM) with a marker of cytokeratin 18 (CK18) and to evaluate the clinical stage of gastric carcinoma and its prognosis. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the expression of CK18 mRNA in 298 lymph nodes from 35 patients with gastric carcinoma and 20 lymph nodes from 10 patients with chronic peptic ulcer and gastric perforation diagnosed by pathological examination and surgery.CK18 mRNA expression of peripheral blood from 54 patients with gastric carcinoma and 10 healthy people were also examined.RESULTS: Expression of CK18 mRNA was not found in 10 patients with benign pathological changes.CK18 mRNA expression in gastric carcinoma tissues was strongly positive. In gastric carcinoma patients,pathological examination revealed that 99 of 298 (33.2%)lymph nodes were positive, while RT-PCR showed that 133 of 298 (44.6%) lymph nodes had expression of CK18 mRNA. The difference was significant (P＜0.05).Among the 199 negative lymph nodes identified by pathological examinations, 34 (17.1%) displayed positive expression of CK18 mRNA by RT-PCR. The positive expression of CK18 mRNA was associated with lymph node micrometastasis (LMM) of gastric carcinoma. CK18 mRNA was negatively expressed in all 10 healthycases and positively expressed in 38.9% of 54 blood specimens from gastric carcinoma patients. The positive rate was not correlated with tumor invasion of gastric carcinoma,but was significantly associated with TNM stage, lymph node metastasis (P=0.0290, P＜ 0.05) and tumor differentiation (P= 0.2956, P＜0.05).CONCLUSION: RT-PCR with CK18 mRNA as a molecular marker is highly sensitive and specific in detecting LMM of gastric carcinoma. It can benefit the diagnosis of MM and guide studies on biological behavior, clinical phase,and therapy as well as relapse monitoring.展开更多
Objective： To investigate the genotypes of well-differentiated non-cardiac gastric adenocarcinoma and their clinicopathological significance. Methods： Sixty-four cases of well-differentiated non-cardiac gastric ade...Objective： To investigate the genotypes of well-differentiated non-cardiac gastric adenocarcinoma and their clinicopathological significance. Methods： Sixty-four cases of well-differentiated non-cardiac gastric adenocarcinoma were included in this study. The expressions of intestinal phenotypic markers including CDX2, MUC2, Li-cadherin, CD10, Hepatocyte（Hep） and Villin, and gastric phenotypic markers including MUC5AC and pS2 were detected immunohistochemically. Based on the expressions of phenotypic markers, 64 cases can be divided into four phenotypes. Cases only expressing intestinal phenotypic markers were classified as intestinal phenotype; cases only expressing gastric phenotypic markers as gastric phenotype; cases expressing both intestinal and gastric phenotypic markers as gastrointestinal phenotype; and cases expressing neither intestinal nor gastric phenotypic marker as null phenotype. The association of phenotype and clinic-pathological parameters was analyzed. We also detected the expressions of markers related to the development and progression of cancer, including Rb, P53, c-Met, MIF, TGF-β-RII, β-catenin, CD44v6 and E-cadherin. Results： Of 64 cases, 33（51.6%） were intestinal type, 3（4.7%） were gastric type, 25（39.1%） were gastrointestinal type and 3（4.7%） were null type. Fifty-eight cases were either intestinal or gastrointestinal type, which accounted for 90.6% of all the cases. In addition, there was an association between phenotype and biological behaviors （invasion or metastasis）. The biological behaviors of intestinal and gastrointestinal type were better than gastric type. Compared with intestinal, gastric and gastrointestinal types, the biological behaviors of null type were the most aggressive. The biological behaviors of gastric carcinoma tended to be better as the number of expression of intestinal markers increased. Expression of markers related to the development and progression of cancer was not significantly correlated with phenotypes and biological behaviors of well-differentiated gastric carcinoma. Conclusion： Well-differentiated gastric adenocarcinomas are heterogeneous phenotypically. They can be divided into four phenotypes, namely intestinal, gastric, gastrointestinal and null types. Present findings show that well-differentiated gastric adenocarcinoma in the WHO classification is highly consistent with intestinal type gastric cancer in the Lauren classification. Expression of phenotypic marker is of certain clinic-pathologic significance.展开更多
Objective:The aim of our study was to investigate the prevalence and clinical relevance of neuroendocrine(NE) differentiation in lung adenocarcinoma.Methods:Eighty-six adenocarcinoma paraffin-embedded specimens and ca...Objective:The aim of our study was to investigate the prevalence and clinical relevance of neuroendocrine(NE) differentiation in lung adenocarcinoma.Methods:Eighty-six adenocarcinoma paraffin-embedded specimens and cases which were followed up completely for 3 years,were obtained from 86 patients(35 men and 51 women) who underwent surgical resection for pathologically supported adenocarcinoma in the Cancer Hospital of Tianjin Medical University,from June 2005 to December 2006.Immunohistochemical EnVision two-step method was used to detect the expression of neuron-specific enolase(NSE),synaptophysin(SYN) and chromogranin A(CGA).All data were analyzed using SPSS statistics software and Kaplan-Meier survival curves were constructed,meanwhile,we conducted a Log-rank test.Results:All patients with lung adenocarcinoma,35 cases with NE differentiation(40.7%).The statistical analysis showed that the positive rate of NE differentiation in lung adenocarcinoma was significantly associated with cancer recurrence and histological differentiation.In addition,CGA,NSE and SYN positive rates were 27.9%,50.0%,43.0%,respectively.A statistically significant difference was found between positive expression of SYN and other clinicopathological parameters,such as pathological type,histological differentiation,lymph node metastasis,postoperative recurrence and 3-year survival rate(P = 0.001) and so on.Conclusion:NE differentiation can be used as a metastatic potentially indicator of biological behavior of lung adenocarcinoma,and combined detection of NSE and SYN markers may be recommended to examine NE differentiation of lung adenocarcinoma.Positive expression of SYN indicates poor prognosis.展开更多
Objective To investigate miR-183-5p targeting to forkhead box protein O1(FOXO1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung canc...Objective To investigate miR-183-5p targeting to forkhead box protein O1(FOXO1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)cells.Methods NSCLC tissues and adjacent normal tissues from 60 patients with NSCLC adenocarcinoma were obtained via pathological biopsy or intraoperative resection.Several cell lines were cultured in vitro,including the human normal lung epithelial cell line BEAS-2B and human NSCLC cell lines A549,SPCA-1,PC-9,and 95-D.miR-183-5p and FOXO1 mRNA expression in tissues and cells were detected by qRT-PCR;the corresponding correlations in NSCLC tissues were analyzed using the Pearson test,and the relationship between miR-183-5p expression and clinicopathological parameters was analyzed.The miR-183-5p-mediated regulation of FOXO1 was verified by bioinformatics prediction alongside double luciferase,RNA-binding protein immunoprecipitation(RIP)assay,and pull-down experiments.A549 cells were divided into control,anti-miR-NC,anti-miR-183-5p,miR-NC,miR-183-5p,miR-183-5p+pcDNA3.1,and miR-183-5p+pcDNA3.1-FOXO1 groups.Cell proliferation,invasion,migration,apoptosis,and cell cycle distribution were detected using an MTT assay,clone formation assay,Transwell assay,scratch test,and flow cytometry,respectively.The expression of EMT-related proteins in the cells was analyzed by western blotting.The effect of miR-185-3p silencing on the development of transplanted tumors was detected by analyzing tumor formation in nude mice.Results miR-183-5p expression was significantly higher in NSCLC tissues and cells than in adjacent normal tissues,whereas FOXO1 mRNA expression was significantly down-regulated.There was a significant negative correlation between miR-183-5p and FOXO1 mRNA in NSCLC tissues(P<0.05).Additionally,the expression of miR-183-5p was significantly correlated with tumor size,tumor differentiation,and tumor-node-metastasis stage in patients with NSCLC(P<0.05).miR-183-5p targeted and inhibited FOXO1 expression.Compared to the anti-miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the anti-miR-183-5p group,whereas the protein expression of E-cadherin andα-catenin and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion were significantly lower in the anti-miR-183-5p group(P<0.05).Compared to the miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells in the miR-183-5p group were significantly higher,whereas the E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly lower;furthermore,the frequency of colony formation and invasion were significantly higher in the miR-183-5p group(P<0.05).Compared with the miR-183-5p+pcDNA3.1 group,the OD value,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group,whereas E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion was significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group(P<0.05).Overall,silencing miR-185-3p inhibited the growth of transplanted tumors and promoted FOXO1 expression.Conclusion Overexpression of miR-183-5p can inhibit apoptosis and promote the proliferation,migration,invasion,and EMT,of NSCLC cells by down-regulating FOXO1 expression.展开更多
The in-vitro biological behavior of a newly-developed iron-containing hydroxyapatite/titanium（HA/Ti） composite was investigated by immersing the composites in the simulated body fluid（SBF） for up to 5 weeks.The ad...The in-vitro biological behavior of a newly-developed iron-containing hydroxyapatite/titanium（HA/Ti） composite was investigated by immersing the composites in the simulated body fluid（SBF） for up to 5 weeks.The addition of iron was observed to have a significant influence on the in-vitro biological behavior of the composites.The obtained results revealed that the stability of the composites in the physiological solution was markedly improved.The solubility decreased in the order of pure HA,HA/5（Ti-33Fe）,and HA/15（Ti33Fe）.Precipitation occurred on the surface of HA/5（Ti-33Fe） composite,showing a good combination of physiostability with bioactivity,while HA/15（Ti-33Fe） composite exhibited superior physiostability since there was no obvious change on the surface of HA/15（Ti-33Fe）.展开更多
BACKGROUND The coronavirus disease 2019(COVID-19)pandemic had a significant impact on the management of all diseases.Various diseases such as cancer have a higher risk of COVID-19-related death.Despite this fact,any d...BACKGROUND The coronavirus disease 2019(COVID-19)pandemic had a significant impact on the management of all diseases.Various diseases such as cancer have a higher risk of COVID-19-related death.Despite this fact,any delay or alteration in treatment of cancer may have fatal consequences.Hepatocellular carcinoma(HCC)is an aggressive liver cancer that requires multimodality treatment to improve survival.AIM To evaluate the impact of COVID-19 on the management of patients with HCC by determining changes in demographic,clinical and histopathological variables.METHODS Demographic,clinical and pathological variables of patients with HCC who had undergone liver transplantation between March 2020 and June 2021(Pandemic group,n=48)were retrospectively compared with that of the patients with HCC transplanted between November 2018 and March 2020(Pre-pandemic group,n=61).RESULTS The median age of the patients in the study was 56(interquartile range=15).Ninety-seven patients(89%)were male and 12 were female(11%).The most common etiology of liver disease was hepatitis B virus(n=52,47.7%).According to our results,there was a 21.3% drop in the number of patients transplanted for HCC.There was no difference in the demographic,clinical and pathological characteristics of the patients except blood alkaline phosphatase levels(P=0.029),lymphovascular invasion(P=0.019)and type of the liver graft that was transplanted(P=0.017).CONCLUSION It is important to develop a surveillance strategy for liver transplant centers.The liver transplantation for HCC is justified and safe provided that strict surveillance protocols are applied.展开更多
基金Supported by The National Natural Science Foundation of China, No. 30972898
文摘AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma cell line PANC-1 and explore their clinical significance.METHODS:The immunohistochemistry and Western blot were used to determine the expression of TLR9 protein in pancreatic cancer tissues,and immunofluorescence staining was performed to detect the TLR9 protein expression in pancreatic carcinoma cell line PANC-1.To assess the effects of CPG ODN2216 on the invasive property of Panc-1 cells,in vitro cell adhesion,wound-healing scrape,and invasion and cell colony formation were evaluated.RESULTS:TLR9 was highly expressed in pancreaticcancer tissues and PANC-1 cells.The percentage of positive cells expressing TLR9 protein in human pancreatic tissues,paracancerous tissues and normal tissues were 73.3%,33.3% and 20.0%,respectively,and the protein expression level of TLR9 was gradually descending(P < 0.05).In vitro tests in wound-healing scrape,cell adhesion,colony formation and matrigel invasion showed that the adhesion and motility of PANC-1 cells in CPG ODN 2216 treatment group were signif icantly lower than in the control group(P < 0.05).The cell growth assay showed that the proliferative ability of PANC-1 cells in treatment group was significantly decreased and CPG ODN2216 had an inhibitive effect in the growth of Panc-1 cells in a dose and time-dependent manner(P < 0.05).CONCLUSION:The gene of TLR9 is correlated with the invasive and metastatic potential of human pancreatic carcinoma,and CPG ODN2216 induces the inhibition of migration and invasion of Panc-1 cells.
基金Supported by the Tianjin Key Medical Discipline(Specialty)Construction Project(No.TJYXZDXK-037A)Tianjin Medical University Eye Hospital。
文摘AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformation(non-HGT)primary cells cultured by non-highgrade transformation tissue in proliferation,metastasis,drug susceptibility,and genes.METHODS:LACC-HGT primary cells were established by tissue block culture,and the 4^(th)to 10^(th)generation primary cells were selected as research objects.The cells were preliminarily identified by immunofluorescent staining.The differences between non-HGT and LACC-HGT primary cells in terms of proliferation,metastasis,and drug susceptibility were compared by cell counting kit-8(CCK-8)assay,wound healing,and drug sensitivity experiments.Differentially expressed genes were screened using mRNA array.Gene expression was analyzed using real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS:LACC-HGT primary cells were successfully cultured by tissue block culture.Immunofluorescence staining results showed that cytokeratin(CK)and CK7 expression levels were positive in LACC-HGT primary cells.CCK-8 results showed that the proliferation ability of LACCHGT cells was significantly higher than that of non-HGT cells.Wound healing experiment showed that the migration ability of LACC-HGT cells was significantly higher than that of non-HGT cells.LACC-HGT cells were also less sensitive to cisplatin and paclitaxel than non-HGT cells.Compared with non-HGT cells,9566 differentially expressed genes were found in LACC-HGT primary cells,of which 5162 were upregulated and 4404 were down-regulated.The expression of N-acetylneuraminate pyruvate lyase(NPL),MARVEL domain containing 3(MARVELD3),syntabulin(SYBU),and allograft inflammatory factor 1(AIF1)was higher in LACCHGT cells than in non-HGT cells,whereas that of periostin(POSTN)was lower.CONCLUSION:LACC-HGT primary cells have faster proliferation,stronger migration ability,and poorer sensitivity to chemotherapy drugs than non-HGT primary cells.The expression of mRNAs in non-HGT and LACC-HGT primary cells are significantly different.These features are speculated to be the reasons why high-grade transformation tissues exhibit higher malignant degree and poorer prognosis than their counterparts.
基金supported by the Research Special Fund for Public Welfare Industry of Health(201402008)
文摘Dopamine agonists （DA） are a first-line therapy for prolactinomas （PA）. However, nearly 10% of prolactinomas do not respond to DA therapy. A considerable number of studies have shown that estrogen plays an important role in the development of prolactinomas. However, the expression of estrogen receptors （ER） in prolactinomas has not been fully explored. Accordingly, we examined the levels of ESR1 and its subtypes A5-DeI-ESR1 and ESR2 mRNA in prolactinomas. In the present study,
文摘<strong><em>Background. </em></strong>The active ingredient curcumin of traditional Chinese medicine was selected as the research object to investigate the possible mechanism of breast cancer metastatic bone pain in mouse walker 256 cells and the effect of curcumin on the NF-κB/TNF-α pathway in order to provide a new idea for clinical treatment of breast cancer metastatic bone pain. <strong><em>Methods.</em></strong> By establishing an animal model of breast cancer bone metastasis in walker 256 cells, the biological behavior of nude mice was observed on the 8th day after successful modeling. Meanwhile, the low dose group, middle dose group and high dose group of mice were given 15 mg/kg, 25 mg/kg, 50 mg/kg of curcumin solution intraperitoneally in 21 days, and the right cavity bone and spinal cord distended in mice (L4-L6) tissues were used to detect related factors, Immunohistochemical method was used to detect c-fos in spinal cord. Expression levels of RANK, NF-κB and TNF-α were detected by RT-PCR and Western blot. Meanwhile, serum levels of Cox2, il-6, leukotriene and PGE2 were detected.<strong><em> Results. </em></strong>Observing the biological behavior index of nude mice, we found that the mechanical pain and thermal pain threshold decreased (p < 0.05), and the cold pain and spontaneous pain scores increased significantly (p < 0.05). After group study, the expression of c-fos in the cancer pain model group was significantly higher than that in the normal control group (p < 0.05), and with the increase of curcumin dose, the expression of c-fos in the high dose group was significantly lower than that in the solvent model group (p < 0.05). The expression of RANK, NF-κB, TNF-α was higher than that of the normal control group and decreased gradually with the increase of curcumin dose, among which the expression of high dose group was significantly lower than that of solvent group (p < 0.05). RANK, NF-κB, TNF-α protein expression was higher than that of normal control group and gradually decreased with the increase of curcumin dose. The levels of Cox2, IL-6, leukotriene and PGE2 in serum decreased with the increase of curcumin dose, and the high dose group decreased significantly (p < 0.05). <em><strong>Conclusions. </strong></em>On the 8th day after the success of the animal model of breast cancer bone metastasis in Walker 256 cells, abnormal biological behaviors such as heat pain, cold pain sensation and spontaneous hyperalgesia were observed. Further studies have found that the increased expression of rank on osteoclasts induced up-regulated expression of NF-κB and c-fos, induced expression of TNF-α gene, and could induce synthesis and release of leukotriene, PGE2 through direct activation of cyclooxygenase, inflammatory media IL-6 cascade reaction, resulting in pathological pain and hypersensitivity. Traditional Chinese medicine active ingredient curcumin could reduce RANK expression of osteoclast, inhibit cell NF-κB and spinal cord c-fos activity, reduce TNF-α expression, inhibit Cox2 activity, and reduce the synthesis and release of inflammatory factors leukotriene and PGE2, thus exerting its analgesic effect, which provides new ideas and methods for clinical treatment of metastatic bone pain in breast cancer.
基金supported by grants from the National Natural Science Foundation of China(81070660)the Science and Technology Project Foundation of Guangdong Province(2008A030201012)+1 种基金Medical Science and Technology Research Foundation of Guangdong Province(A2012183)the Science and Technology Project Foundation of Guangdong Province(2009B091300128)
文摘BACKGROUND: The high level of matrix metalloproteinase 9(MMP9) is thought to slow down the healing of diabetic foot ulcers. Whether it can influence the biological behaviors of skin fi broblasts and affect wound healing is still unclear. The present study aimed to observe changes in the biological behaviors of rat dermal fi broblasts induced by high expression of MMP9 and to clarify the possible mechanisms of wound healing for diabetic foot.METHODS: A cell model of skin f ibroblast with high expression of MMP9 was established by coculture of high glucose(22.0 mmol/L) and homocysteine(100 μmol/L). A control group was incubated with normal glucose(5.5 mmol/L). Realtime PCR, ELISA and gelatin zymography were used to detect the MMP9 mRNA, protein expression and activity of MMP9. Flow cytometry, CCK-8, ELISA assay, scratch test and transwell were used to detect cell proliferation, viability, collagen(hydroxyproline) secretion, horizontal migration and vertical migration of cells. The data were expressed as mean±SD. P value less than 0.05 was considered statistically signif icant.RESULTS: The expression of MMP9 mRNA, protein levels and the activity of MMP9 were much higher in the high MMP9 group than in the control group(7.05±1.02 vs. 1.00±0.00, 206.9±33.6 pg/mL vs. 40.4±5.9 pg/mL, and 1.47±0.13 vs. 0.57±0.12, respectively, P<0.01). The proportion of S-phase cells, proliferation index, cell viability, collagen(hydroxyproline) secretion, horizontal migration rate and the number of vertical migration cells were lower in the high MMP9 group than in the control group(P<0.01).CONCLUSION: Fibroblasts with a high expression of MMP9 decreased proliferation, activity, secretion and migration of collagens, suggesting that MMP9 may inhibit the biological behaviors of fi broblasts.
基金Supported by Projects of the National Natural Science Foundation of China,No.81873915,No.31872738 and No.81673241Key Plan of Nantong S&T Development,No.MS12020021Program of Medical School S&T of Nantong University,No.2018YFC0116902。
文摘BACKGROUND Insulin-like growth factor-1 receptor(IGF-1R)is over-expressed in hepatocellular carcinoma(HCC).However,the relationship between IGF-1R activation and HCC progression remains unidentified.AIM To investigate the effects of editing IGF-1R on the biological features of HCC cells.METHODS Immunohistochemistry analyzed the expressions of IGF-1R and P-glyco protein(P-gp)in HCC tissues and their distal non-cancerous tissues(non-Ca).IGF-1R was edited with Crispr/Cas9 system,screened specific sg RNAs,and then transfected into Hep G2 cells.CCK-8,scratch wound test detected cell proliferation,migration,invasion and transwell assays,respectively.Alterations of IGF-1R and P-gp were confirmed by Western blotting.Alterations of anti-cancer drug IC_(50)values were analyzed at the cell level.RESULTS The positive rates of IGF-1R(93.6%,χ~2=63.947)or P-gp(88.2%,χ~2=58.448)were significantly higher(P<0.001)in the HCC group than those(36.6%in IGF-1R or 26.9%in P-gp)in the non-Ca group.They were positively correlated between high IGF-1R and P-gp expression,and they were associated with hepatitis B virus infection and vascular invasion of HCC.Abnormal expressions of circulating IGF-1R and P-gp were confirmed and associated with HCC progression.Biological feature alterations of HCC cells transfected with specific sg RNA showed IGF-1R expression down-regulation,cell proliferation inhibition,cell invasion or migration potential decreasing,and enhancing susceptibility of Hep G2 cells to anti-cancer drugs.CONCLUSION Edited oncogenic IGF-1R was useful to inhibit biological behaviors of Hep G2 cells.
基金The work was supported by a grant from the National Natural Science Foundation of China （No.30970843）
文摘OBJECTIVE To observe the effects expression and activation on biological cancer stem cells. of blocking CD133 gene characteristic of the colon METHODS CD133＋ colon cancer stem cells （CCSCs） were separated from EpCAMhigh CD44＋ CCSCs through fluorescenceactivated cell sorting （FACS）. The proliferation, the capability of spherical cell formation, neoplasia, and the expression of ABCG2 mRNA of CD133＋ CCSCs were observed after the CD133＋ CCSCs were infected with LV-CD133shRNA. CD133 negative cells were isolated from EpCAMhigh CD44＋ CCSCs with FACS, and the CD133 proteins in CD133- cells were detected with Western blot. RESULTS CD133＋ CCSCs were isolated from EpCAMhigh CD44＋ CCSCs using FACS, and they accounted for 89.2% in the stem cells. In the experimental group, after the CD133＋ CCSCs were knocked down by LV-CD133shRNA RNAi, the growth pattern of the cells in the stem cell culture changed into adherent growth from suspended growth, and couldn＇t generate spherical cells. Results of MTT assay showed that the CD133＋ CCSCs infected with LV-CD133shRNA grew slowly, compared to the cells in the control groups. There was a decrease in the cloning efficiency. The infected cells were transplanted into the BALB/c nude mice. During the observation, no neoplasia was found in the CD133＋ cells infected with LV-CD133shRNA. The level of ABCG2 mRNA expression was lowered greatly （P 〈 0.01）. CD133- cells were obtained from the EpCAMhigh CD44＋ CCSCs using FACS, in which the expression of CD133 protein was positive. CONCLUSION CD133 retains the biological characteristics of the colon cancer stem cells.
基金Research and Development Key Project of Hainan Province(No.ZDYF2020134,ZDYF2022SHFZ283)the National Natural Science Foundation of China(No.81660489)+1 种基金the Special Research Fund of The Innovation Platform for Academicians of Hannan Province(No.YSPTZX202005)Innovative Research Project for Postgraduates of Hainan Province(No.Hys2020-355)。
文摘Objective:To investigate the effect of lentiviral stable high expression of circLIFR on the biological behavior of Hep3B hepatocellular carcinoma(HCC)cells.Methods:Hep3B cell lines were infected with lentiviral packaging of circLIFR expression plasmids to construct stable circLIFR high expression HCC cells.The lentivirus infected with circLIFR high expression sequence was used as the circLIFR high expression group,the lentivirus infected with circLIFR high expression empty vector sequence was used as the negative control group,and the uninfected group was used as the blank control group.After that,circLIFR expression levels were detected by qPCR,and the back splice sites were identified by Sanger sequencing.The cell viability was examined by cell proliferation kit and invasive ability was determined by Transwell assay.Results:The qPCR and Sanger sequencing showed that the stable circLIFR expression of Hep3B cells was successfully established.The circLIFR high expression group had better cell proliferation viability than the negative control and blank control groups,and the differences were statistically significant(P<0.05).The number of cells crossing Matrigel gel in the negative control and blank control groups was(270.8±18.9)and(266.2±17.6),respectively,while the number of cells crossing Matrigel gel in the circLIFR high expression group was(396.6±32.9),and the differences were statistically significant(P<0.05).Conclusion:High circLIFR expression considerably promotes the proliferation and invasive ability of Hep3B cells.
基金Natural Science Foundation of Hunan Province(2021JJ30017)Key Project of Traditional Chinese Medicine Research Program of Hunan Province(C2022001)+1 种基金Key Project of Education Department of Hunan Province(21A0226)Changsha City Natural Science Foundation Project(kq2208184)。
文摘Obiective:To investigate the effects of Xiaoxianxiong Tang on the proliferation,invasion,apoptosis,and other cell biological behaviors of non-small cell lung cancer A549 cells.Methods:Human lung adenocarcinoma A549 cells were cultured in vitro,and the IC50 concentration and effective time of administration of Xiaoxianxiong Tang were determined by the CCK-8 assay to detect the inhibitory effect of Xiaoxianxiong Tang on A549 cells proliferation.The effect of the Xiaoxianxiong Tang on apoptosis was determined by flow cytometry;the apoptosis-related protein was detected via Western blot;the metastasis-related protein mRNA was detected by RT-PCR.Results:Xiaoxianxiong Tang significantly inhibited the proliferation viability,the invasive ability,and the clonogenic ability of A549 cells compared with the control group(P<0.001).Moreover,Xiaoxianxiong Tang significantly promoted the apoptosis of A549 cells(P<0.001).Xiaoxianxiong Tang significantly up-regulated Bax and down-regulated Bcl2 expression in A549 cells compared with the control group(P<0.01).The mRNA expression of MMP2 and MMP9 was significantly down-regulated by Xiaoxianxiong Tang compared with the control group(P<0.05).Conclusion:Xiaoxianxiong Tang has the effect of regulating the biological behavior of A549 cells,and Xiaoxianxiong Tang significantly inhibites the proliferation viability,colony formation,and invasion ability of lung cancer A549 cells.
文摘Objective To characterize and compare the different biological behaviors of two novel human osteosarcoma cell lines,Zos and Zos-M,established respectively from the primary site and the skip metastasis of an osteosarcoma patient.Methods Two
基金Doctoral Fund Project of First Affiliated Hospital,School of Medicine,Shihezi University,China,Grant/Award Number:BS202110。
文摘Background:Bladder cancer(BLCA)is the most prevalent malignancy in the urinary tract system,while ST3GAL5 is a protein-coding gene that catalyzes the formation of ganglioside monosialodihexosylganglioside 3(GM3)synthase.GM3 synthase has been reported to significantly influence the malignant process of various cancers.However,the expression profile and functional role of ST3GAL5 specifucally in BLCA remain to be elucidated.Methods:In order to investigate the relationship between ST3GAL5 expression and malignant biological behavior and prognosis in BLCA.The mRNA expression of ST3GAL5 and clinicopathological characteristics in BLCA were firstly evaluated by public databases.Next,immunohistochemical staining was performed to analyze the protein expression of ST3GAL5 in BLCA and paracancerous tissues,as well as the expression of various types of malignant biological behavior.Subsequently,the gene set enrichment analyses(GSEA)were performed for ST3GAL5 and all correlated genes in BLCA by sorting Pearson's Correlation Coefficient.GSEA was also used to validate the pathways affected by the different expression levels of ST3GAL5 in BLCA patients.Results:The mRNA and protein expression of ST3GAL5 in BLCA were significantly higher in low-grade and non-muscle-invasive BLCA(p<0.05).The results from bioinformatics databases indicated that upregulation of ST3GAL5 have a lower grade,lower pathological stage,less susceptibility to lymphatic metastasis,and lower mortality rates.Kaplan–Meier survival analysis demonstrated that upregulation of ST3GAL5 was associated with better survival in BLCA(p<0.05).Conclusion:Upregulation of ST3GAL5 may be related to tumor suppression in BLCA,and may be a potential prognostic and therapeutic marker for BLCA.
基金supported by the National Natural Science Foundation of China(Grant No.:82272749)the Natural Science Foundation of Liaoning Province,China(Grant No.:2022-MS-190).
文摘Mitochondria play a crucial role as organelles,managing several physiological processes such as redox balance,cell metabolism,and energy synthesis.Initially,the assumption was that mitochondria primarily resided in the host cells and could exclusively transmit from oocytes to offspring by a mechanism known as vertical inheritance of mitochondria.Recent scholarly works,however,suggest that certain cell types transmit their mitochondria to other developmental cell types via a mechanism referred to as intercellular or horizontal mitochondrial transfer.This review details the process of which mitochondria are transferred across cells and explains the impact of mitochondrial transfer between cells on the efficacy and functionality of cancer cells in various cancer forms.Specifically,we review the role of mitochondria transfer in regulating cellular metabolism restoration,excess reactive oxygen species(ROS)generation,proliferation,invasion,metastasis,mitophagy activation,mitochondrial DNA(mtDNA)inheritance,immune system modulation and therapeutic resistance in cancer.Additionally,we highlight the possibility of using intercellular mitochondria transfer as a therapeutic approach to treat cancer and enhance the efficacy of cancer treatments.
文摘Objective:To develop an engineered therapeutic tool based on miR-214,analyze the effects of regulating miR-214 on the biological behavior of cervical cancer cells,and explore its underlying mechanisms.Methods:Two types of miR-214 nano-paper carriers were self-designed according to different ratios.The stability,cellular uptake rate,and targeting ability of different carriers were tested.Human cervical epithelial cells and C6330 He La human cervical cancer cells were cultured.The human cervical epithelial cells in the logarithmic growth phase were used as the blank group.The C6330 He La human cervical cancer cells in the logarithmic growth phase were randomly divided into five groups.Cells from each group were seeded in cell culture plates.The blank group and the model group received no treatment.The miR-214 inhibition group was transfected with miR-214 inhibitor.The miR-214 activation group was delivered miR-214 by high-performance nano-lipid carriers.The MEK3 knockout group was transfected with miR-214 mimic after MEK3 gene knockout.The relative expression levels of miR-214 in each group of cells at 24,48,and 72 h after transfection were detected by real-time fluorescence quantitative PCR.The cell proliferation ability was evaluated by measuring the absorbance(OD)value using the MTT colorimetric method.The cell migration ability was evaluated by the cell scratch assay,and the cell apoptosis rate was evaluated by cell apoptosis detection.Results:The particle size of the 4∶5∶1 ratio carrier was significantly smaller than that of the 7∶2∶1 ratio carrier,while the Zeta potential,encapsulation efficiency,fluorescence intensity,and relative expression level of miR-214 were significantly higher than those of the 7∶2∶1 ratio carrier(P<0.05).After miR-214 inhibition and activation treatment in cervical cancer cells,the relative expression levels of miR-214 in cervical cancer cells decreased and increased respectively,and MEK3 knockout did not affect the change in the relative expression level of miR-214.The OD value of the miR-214 inhibition group was significantly higher than that of the model group,and the OD values of the miR-214 activation group and the MEK3 knockout group were significantly lower than that of the model group(P<0.05).The OD value of the miR-214 activation group was significantly lower than that of the MEK3 knockout group(P<0.05).After miR-214 inhibition treatment in cervical cancer cells,the cell migration ability was significantly enhanced,and the cell apoptosis rate was significantly reduced.After miR-214 activation treatment,the cell migration ability was significantly reduced,and the cell apoptosis rate was significantly increased.However,after knocking out the MEK3 gene,the cell proliferation,migration,and apoptosis abilities all decreased.Conclusion:Activating miR-214 can positively affect the biological behavior of cervical cancer cells.In this study,a nano-paper carrier was self-designed through engineering means,which can achieve efficient delivery of miR-214 and provide a new technological platform for the treatment of cervical cancer.
文摘Our previous studies revealed that second malevibration signal (SMVS) restrained the matingbehavior of N. lugens, the influences of threebiological features (density, age, and wingform) on SMVS’s inhibitory effect were hereinstudied by playing back its record. The dura-tion of playback was 4 h. Except otherwisestatement, N. lugens tested were virginmacropterous males and females aged 4-6 d af-ter emergence, and the density was 5 pairs (5females and 5 males) of N. lugens per cage (4cm in diameter and 8 cm in height). The in-hibitory effect of SMVS was evaluated usingmating rate (i. e. the rate of females withspermatophore). The results were as follows:
基金Supported by the Natural Science Foundation of Jilin Province,No. 20010594
文摘AIM: To explore the biological behavior of gastric carcinoma micrometastasis (MM) with a marker of cytokeratin 18 (CK18) and to evaluate the clinical stage of gastric carcinoma and its prognosis. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the expression of CK18 mRNA in 298 lymph nodes from 35 patients with gastric carcinoma and 20 lymph nodes from 10 patients with chronic peptic ulcer and gastric perforation diagnosed by pathological examination and surgery.CK18 mRNA expression of peripheral blood from 54 patients with gastric carcinoma and 10 healthy people were also examined.RESULTS: Expression of CK18 mRNA was not found in 10 patients with benign pathological changes.CK18 mRNA expression in gastric carcinoma tissues was strongly positive. In gastric carcinoma patients,pathological examination revealed that 99 of 298 (33.2%)lymph nodes were positive, while RT-PCR showed that 133 of 298 (44.6%) lymph nodes had expression of CK18 mRNA. The difference was significant (P＜0.05).Among the 199 negative lymph nodes identified by pathological examinations, 34 (17.1%) displayed positive expression of CK18 mRNA by RT-PCR. The positive expression of CK18 mRNA was associated with lymph node micrometastasis (LMM) of gastric carcinoma. CK18 mRNA was negatively expressed in all 10 healthycases and positively expressed in 38.9% of 54 blood specimens from gastric carcinoma patients. The positive rate was not correlated with tumor invasion of gastric carcinoma,but was significantly associated with TNM stage, lymph node metastasis (P=0.0290, P＜ 0.05) and tumor differentiation (P= 0.2956, P＜0.05).CONCLUSION: RT-PCR with CK18 mRNA as a molecular marker is highly sensitive and specific in detecting LMM of gastric carcinoma. It can benefit the diagnosis of MM and guide studies on biological behavior, clinical phase,and therapy as well as relapse monitoring.
基金supported by grants from Beijing Municipal Science & Technology commission NOVA Program(No.2005B-44)the Key Technology Research and Development Program(No. 2002BA711A06)+1 种基金the National"973"Basic Research Program of China(No.1998051203)the National"863"High-Tech Res & Dev Program of China
文摘Objective： To investigate the genotypes of well-differentiated non-cardiac gastric adenocarcinoma and their clinicopathological significance. Methods： Sixty-four cases of well-differentiated non-cardiac gastric adenocarcinoma were included in this study. The expressions of intestinal phenotypic markers including CDX2, MUC2, Li-cadherin, CD10, Hepatocyte（Hep） and Villin, and gastric phenotypic markers including MUC5AC and pS2 were detected immunohistochemically. Based on the expressions of phenotypic markers, 64 cases can be divided into four phenotypes. Cases only expressing intestinal phenotypic markers were classified as intestinal phenotype; cases only expressing gastric phenotypic markers as gastric phenotype; cases expressing both intestinal and gastric phenotypic markers as gastrointestinal phenotype; and cases expressing neither intestinal nor gastric phenotypic marker as null phenotype. The association of phenotype and clinic-pathological parameters was analyzed. We also detected the expressions of markers related to the development and progression of cancer, including Rb, P53, c-Met, MIF, TGF-β-RII, β-catenin, CD44v6 and E-cadherin. Results： Of 64 cases, 33（51.6%） were intestinal type, 3（4.7%） were gastric type, 25（39.1%） were gastrointestinal type and 3（4.7%） were null type. Fifty-eight cases were either intestinal or gastrointestinal type, which accounted for 90.6% of all the cases. In addition, there was an association between phenotype and biological behaviors （invasion or metastasis）. The biological behaviors of intestinal and gastrointestinal type were better than gastric type. Compared with intestinal, gastric and gastrointestinal types, the biological behaviors of null type were the most aggressive. The biological behaviors of gastric carcinoma tended to be better as the number of expression of intestinal markers increased. Expression of markers related to the development and progression of cancer was not significantly correlated with phenotypes and biological behaviors of well-differentiated gastric carcinoma. Conclusion： Well-differentiated gastric adenocarcinomas are heterogeneous phenotypically. They can be divided into four phenotypes, namely intestinal, gastric, gastrointestinal and null types. Present findings show that well-differentiated gastric adenocarcinoma in the WHO classification is highly consistent with intestinal type gastric cancer in the Lauren classification. Expression of phenotypic marker is of certain clinic-pathologic significance.
文摘Objective:The aim of our study was to investigate the prevalence and clinical relevance of neuroendocrine(NE) differentiation in lung adenocarcinoma.Methods:Eighty-six adenocarcinoma paraffin-embedded specimens and cases which were followed up completely for 3 years,were obtained from 86 patients(35 men and 51 women) who underwent surgical resection for pathologically supported adenocarcinoma in the Cancer Hospital of Tianjin Medical University,from June 2005 to December 2006.Immunohistochemical EnVision two-step method was used to detect the expression of neuron-specific enolase(NSE),synaptophysin(SYN) and chromogranin A(CGA).All data were analyzed using SPSS statistics software and Kaplan-Meier survival curves were constructed,meanwhile,we conducted a Log-rank test.Results:All patients with lung adenocarcinoma,35 cases with NE differentiation(40.7%).The statistical analysis showed that the positive rate of NE differentiation in lung adenocarcinoma was significantly associated with cancer recurrence and histological differentiation.In addition,CGA,NSE and SYN positive rates were 27.9%,50.0%,43.0%,respectively.A statistically significant difference was found between positive expression of SYN and other clinicopathological parameters,such as pathological type,histological differentiation,lymph node metastasis,postoperative recurrence and 3-year survival rate(P = 0.001) and so on.Conclusion:NE differentiation can be used as a metastatic potentially indicator of biological behavior of lung adenocarcinoma,and combined detection of NSE and SYN markers may be recommended to examine NE differentiation of lung adenocarcinoma.Positive expression of SYN indicates poor prognosis.
文摘Objective To investigate miR-183-5p targeting to forkhead box protein O1(FOXO1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)cells.Methods NSCLC tissues and adjacent normal tissues from 60 patients with NSCLC adenocarcinoma were obtained via pathological biopsy or intraoperative resection.Several cell lines were cultured in vitro,including the human normal lung epithelial cell line BEAS-2B and human NSCLC cell lines A549,SPCA-1,PC-9,and 95-D.miR-183-5p and FOXO1 mRNA expression in tissues and cells were detected by qRT-PCR;the corresponding correlations in NSCLC tissues were analyzed using the Pearson test,and the relationship between miR-183-5p expression and clinicopathological parameters was analyzed.The miR-183-5p-mediated regulation of FOXO1 was verified by bioinformatics prediction alongside double luciferase,RNA-binding protein immunoprecipitation(RIP)assay,and pull-down experiments.A549 cells were divided into control,anti-miR-NC,anti-miR-183-5p,miR-NC,miR-183-5p,miR-183-5p+pcDNA3.1,and miR-183-5p+pcDNA3.1-FOXO1 groups.Cell proliferation,invasion,migration,apoptosis,and cell cycle distribution were detected using an MTT assay,clone formation assay,Transwell assay,scratch test,and flow cytometry,respectively.The expression of EMT-related proteins in the cells was analyzed by western blotting.The effect of miR-185-3p silencing on the development of transplanted tumors was detected by analyzing tumor formation in nude mice.Results miR-183-5p expression was significantly higher in NSCLC tissues and cells than in adjacent normal tissues,whereas FOXO1 mRNA expression was significantly down-regulated.There was a significant negative correlation between miR-183-5p and FOXO1 mRNA in NSCLC tissues(P<0.05).Additionally,the expression of miR-183-5p was significantly correlated with tumor size,tumor differentiation,and tumor-node-metastasis stage in patients with NSCLC(P<0.05).miR-183-5p targeted and inhibited FOXO1 expression.Compared to the anti-miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the anti-miR-183-5p group,whereas the protein expression of E-cadherin andα-catenin and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion were significantly lower in the anti-miR-183-5p group(P<0.05).Compared to the miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells in the miR-183-5p group were significantly higher,whereas the E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly lower;furthermore,the frequency of colony formation and invasion were significantly higher in the miR-183-5p group(P<0.05).Compared with the miR-183-5p+pcDNA3.1 group,the OD value,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group,whereas E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion was significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group(P<0.05).Overall,silencing miR-185-3p inhibited the growth of transplanted tumors and promoted FOXO1 expression.Conclusion Overexpression of miR-183-5p can inhibit apoptosis and promote the proliferation,migration,invasion,and EMT,of NSCLC cells by down-regulating FOXO1 expression.
基金supported by the Fundamental Research Funds for the Central Universities (N090602001)the Natural Sciences and Engineering Research Council of Canada (NSERC)
文摘The in-vitro biological behavior of a newly-developed iron-containing hydroxyapatite/titanium（HA/Ti） composite was investigated by immersing the composites in the simulated body fluid（SBF） for up to 5 weeks.The addition of iron was observed to have a significant influence on the in-vitro biological behavior of the composites.The obtained results revealed that the stability of the composites in the physiological solution was markedly improved.The solubility decreased in the order of pure HA,HA/5（Ti-33Fe）,and HA/15（Ti33Fe）.Precipitation occurred on the surface of HA/5（Ti-33Fe） composite,showing a good combination of physiostability with bioactivity,while HA/15（Ti-33Fe） composite exhibited superior physiostability since there was no obvious change on the surface of HA/15（Ti-33Fe）.
文摘BACKGROUND The coronavirus disease 2019(COVID-19)pandemic had a significant impact on the management of all diseases.Various diseases such as cancer have a higher risk of COVID-19-related death.Despite this fact,any delay or alteration in treatment of cancer may have fatal consequences.Hepatocellular carcinoma(HCC)is an aggressive liver cancer that requires multimodality treatment to improve survival.AIM To evaluate the impact of COVID-19 on the management of patients with HCC by determining changes in demographic,clinical and histopathological variables.METHODS Demographic,clinical and pathological variables of patients with HCC who had undergone liver transplantation between March 2020 and June 2021(Pandemic group,n=48)were retrospectively compared with that of the patients with HCC transplanted between November 2018 and March 2020(Pre-pandemic group,n=61).RESULTS The median age of the patients in the study was 56(interquartile range=15).Ninety-seven patients(89%)were male and 12 were female(11%).The most common etiology of liver disease was hepatitis B virus(n=52,47.7%).According to our results,there was a 21.3% drop in the number of patients transplanted for HCC.There was no difference in the demographic,clinical and pathological characteristics of the patients except blood alkaline phosphatase levels(P=0.029),lymphovascular invasion(P=0.019)and type of the liver graft that was transplanted(P=0.017).CONCLUSION It is important to develop a surveillance strategy for liver transplant centers.The liver transplantation for HCC is justified and safe provided that strict surveillance protocols are applied.
